Click here to switch to the map view.

The map label for this gene is potF [H]

Identifier: 13471137

GI number: 13471137

Start: 851649

End: 852746

Strand: Reverse

Name: potF [H]

Synonym: mll1024

Alternate gene names: 13471137

Gene position: 852746-851649 (Counterclockwise)

Preceding gene: 13471139

Following gene: 13471135

Centisome position: 12.12

GC content: 56.65

Gene sequence:

>1098_bases
ATGATCCGCAAGGCGCTTCTTTGGTCGGCTGGATCGTTACTTGCCGGGCTCATGACCCTGTCCGCGCAAGCTGCCGACAA
GGTGGTCAACCTTTACGACTGGTCGGACTACCTGGATCCCACGGTCATCGACGACTTCACCAAGGAAACCGGTATAAAGG
TCGTGTACGACGTTTTTGACTCCAACGAGATCCTCGAGACCAAGCTGCTTACTGGCGGCTCCGGCTACGACGTCGTCGTG
CCGAGCGCCAGTTTCCTGGCCCGCCAGGTCGAAGCCGGCGTCTTTCAGAAGCTGGACAAATCGAAGTTGCCCAACATCGC
CAACCTGTGGGACGTCATCGAGCAGCGCACCGCCAAGTACGACCCGGACAACCAATATTCCGTGAACTACATGTGGGGCA
CGGTGGGTTTGGGGTACAACACCTCGAAGATCAAAGCTGCGCTTGGTGTCGACAGACTCGACAGTTGGGACGTCTTCTTC
GATCCCGCGAAGCTGAAGAAGCTTGCCAGTTGCGGAGTCTATGTGGTCGATTCGCCCACCGAAATCCTGCCCATCACGAT
GAATTATCTTGGACTGAACCCGGAGAGCACCTCGCCGGAAGACTTCGGTAAGGCCGAAGAGGCGTTGATGCGTATCCGCC
CCGACGTCCGCAAAATCGAGTCGTCCGGGTACATCAACGCTTTGGCGGACGGCGATATTTGCCTCGCGATTGGCTGGTCG
GGCGACGTCCTGAAGGCGCGCGATCGCGCCGCTCAAGCCGGTAACGGCATCACAATCGCCTACTCGGTACCTCGGGAAGG
CACCGTCATGTGGTTCGACCAACTGGCAATCCCAAAAGATGCACCGCACGCGGACGAAGCGACTGCGTTCATCAACTACT
TGATGAAGCCTGAAGTCGCCGCCAAGAACACGAATTATGTGTTTTTCGCCAATGGCAACAAAGCTTCACAAAAGTTCGTT
GAAAGAAGCATTCTTGGCGACCCGGCGATCTATCCAGACGATGCCACCCTAAAGAAGCTTTTCACAGTCTTTCCGTACGA
TCAGAAGACGCAGCGCGTGGCTACGCGCACCTGGACGAAAATCGTGACGGGTCACTGA

Upstream 100 bases:

>100_bases
GCGGGGCACATTCCCTTCATCTGTGCTAATATAGCAACCATGCGCGGCCGTGTACAAAGTACGGCATTCGCATGTTGGGA
CTGCAACCAATGGGGAGCAC

Downstream 100 bases:

>100_bases
TTGGCGGCAGTTTCGCTTCGGAGTTCATCGCTCATCCGTCAATCCGCAATGCCTGCTTTCCGCAGGCCCTCCAGCCAGTG
CTCCAAGTCCTCTTGGCGGC

Product: polyamine transport protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MIRKALLWSAGSLLAGLMTLSAQAADKVVNLYDWSDYLDPTVIDDFTKETGIKVVYDVFDSNEILETKLLTGGSGYDVVV
PSASFLARQVEAGVFQKLDKSKLPNIANLWDVIEQRTAKYDPDNQYSVNYMWGTVGLGYNTSKIKAALGVDRLDSWDVFF
DPAKLKKLASCGVYVVDSPTEILPITMNYLGLNPESTSPEDFGKAEEALMRIRPDVRKIESSGYINALADGDICLAIGWS
GDVLKARDRAAQAGNGITIAYSVPREGTVMWFDQLAIPKDAPHADEATAFINYLMKPEVAAKNTNYVFFANGNKASQKFV
ERSILGDPAIYPDDATLKKLFTVFPYDQKTQRVATRTWTKIVTGH

Sequences:

>Translated_365_residues
MIRKALLWSAGSLLAGLMTLSAQAADKVVNLYDWSDYLDPTVIDDFTKETGIKVVYDVFDSNEILETKLLTGGSGYDVVV
PSASFLARQVEAGVFQKLDKSKLPNIANLWDVIEQRTAKYDPDNQYSVNYMWGTVGLGYNTSKIKAALGVDRLDSWDVFF
DPAKLKKLASCGVYVVDSPTEILPITMNYLGLNPESTSPEDFGKAEEALMRIRPDVRKIESSGYINALADGDICLAIGWS
GDVLKARDRAAQAGNGITIAYSVPREGTVMWFDQLAIPKDAPHADEATAFINYLMKPEVAAKNTNYVFFANGNKASQKFV
ERSILGDPAIYPDDATLKKLFTVFPYDQKTQRVATRTWTKIVTGH
>Mature_365_residues
MIRKALLWSAGSLLAGLMTLSAQAADKVVNLYDWSDYLDPTVIDDFTKETGIKVVYDVFDSNEILETKLLTGGSGYDVVV
PSASFLARQVEAGVFQKLDKSKLPNIANLWDVIEQRTAKYDPDNQYSVNYMWGTVGLGYNTSKIKAALGVDRLDSWDVFF
DPAKLKKLASCGVYVVDSPTEILPITMNYLGLNPESTSPEDFGKAEEALMRIRPDVRKIESSGYINALADGDICLAIGWS
GDVLKARDRAAQAGNGITIAYSVPREGTVMWFDQLAIPKDAPHADEATAFINYLMKPEVAAKNTNYVFFANGNKASQKFV
ERSILGDPAIYPDDATLKKLFTVFPYDQKTQRVATRTWTKIVTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=362, Percent_Identity=49.4475138121547, Blast_Score=374, Evalue=1e-105,
Organism=Escherichia coli, GI1787367, Length=322, Percent_Identity=32.2981366459627, Blast_Score=178, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40316; Mature: 40316

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRKALLWSAGSLLAGLMTLSAQAADKVVNLYDWSDYLDPTVIDDFTKETGIKVVYDVFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHCCCEEEEEECC
SNEILETKLLTGGSGYDVVVPSASFLARQVEAGVFQKLDKSKLPNIANLWDVIEQRTAKY
CCCEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DPDNQYSVNYMWGTVGLGYNTSKIKAALGVDRLDSWDVFFDPAKLKKLASCGVYVVDSPT
CCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEECHHHHHHHHHCCEEEECCCC
EILPITMNYLGLNPESTSPEDFGKAEEALMRIRPDVRKIESSGYINALADGDICLAIGWS
CEEEEEEEEEECCCCCCCCHHCCHHHHHHHHHCCHHHHHHCCCCEEEECCCCEEEEECCC
GDVLKARDRAAQAGNGITIAYSVPREGTVMWFDQLAIPKDAPHADEATAFINYLMKPEVA
CCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCC
AKNTNYVFFANGNKASQKFVERSILGDPAIYPDDATLKKLFTVFPYDQKTQRVATRTWTK
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
IVTGH
HCCCC
>Mature Secondary Structure
MIRKALLWSAGSLLAGLMTLSAQAADKVVNLYDWSDYLDPTVIDDFTKETGIKVVYDVFD
CCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCHHHCCCHHHHHHHHHCCCEEEEEECC
SNEILETKLLTGGSGYDVVVPSASFLARQVEAGVFQKLDKSKLPNIANLWDVIEQRTAKY
CCCEEEEEEEECCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
DPDNQYSVNYMWGTVGLGYNTSKIKAALGVDRLDSWDVFFDPAKLKKLASCGVYVVDSPT
CCCCCEEEEEEEEEEECCCCHHHHHHHHCCCCCCCCCEEECHHHHHHHHHCCEEEECCCC
EILPITMNYLGLNPESTSPEDFGKAEEALMRIRPDVRKIESSGYINALADGDICLAIGWS
CEEEEEEEEEECCCCCCCCHHCCHHHHHHHHHCCHHHHHHCCCCEEEECCCCEEEEECCC
GDVLKARDRAAQAGNGITIAYSVPREGTVMWFDQLAIPKDAPHADEATAFINYLMKPEVA
CCHHHHHHHHHHCCCCEEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHCCCCC
AKNTNYVFFANGNKASQKFVERSILGDPAIYPDDATLKKLFTVFPYDQKTQRVATRTWTK
CCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHH
IVTGH
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]