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The map label for this gene is gspE [C]

Identifier: 13471029

GI number: 13471029

Start: 718856

End: 720277

Strand: Reverse

Name: gspE [C]

Synonym: mll0895

Alternate gene names: 13471029

Gene position: 720277-718856 (Counterclockwise)

Preceding gene: 13471030

Following gene: 13471028

Centisome position: 10.24

GC content: 62.59

Gene sequence:

>1422_bases
ATGACCAGCCGTTTTTCCACCCTGCAGAACCGCGACGCGCGTCCGCAGCGCTCGGCGGAGTCAACGCCGGTCGCGCATCA
TGCGGTCGTCATCCCGACCAGCCGGAAGGCTTTGCCGGCAAAGCCCGATGTCGCGCCGGCAAAGAACGCCAACAAGGTGC
TCGATGCGCGCGTGCGCATTCACCGCATGCTGCTCGAGGAAATCAACCTGGTGGCGCTGGAGCGTTTGCCCAAGGATGAG
ATGCGCCGTCAGGTGCATGATTTCGTTTCGGAAAAGACCCGCCAGGAGCGGATGGCGATCAACACCGCCGAACTCGAAGC
GCTGGTCGACGATATCGTCGACGAGATGGTCGGTCTTGGCCCGCTCGAGCCGTTGCTCAAGGATCCCGAAATCAACGACA
TCCTGATCAACGGCCACCAGAACTGCTTCATCGAAAAGAAGGGCAAGTTGCAGCAGGTCCATATCCCGTTCAAGGATGAG
GCGCATCTGCTTAGAATTATTAACAAAATCGTCGCCGCGGTTGGCCGTCGCGTCGACGAATCGCAGCCGATGGTCGATGC
CCGTATGCTGGACGGCTCGCGCTTCAACGCGGCCATCCGCCCGGTTGGCGTCGACGGGCCGCTGGTGTCGATCCGCAAAT
TCTCCAAGAACAAGCTCGGCCTGCACAAGCTGGTCGAATTCGGCGCCATCACCCAGAACATGGCCGAAGTGCTGGCGGCG
GCCGTGCACGCCCGCAAGACGACGATCATTTCGGGCGGCACCGGCACCGGCAAGACGACGATGCTCAACGCGTTGTCGGC
CTTCATTCCCGAAGACGAGCGGTTGATCACCATCGAGGACGCGGCCGAACTTCAGCTGCAGCAGCCGCATGTCGCGCGCA
TGGAAACGCGCCCCGCCAACATCGAGGGCCATGGCGAACTCAAGCAGCGCGATCTCGTCAAAAACGCGCTGCGCATGCGT
CCCGACCGCGTCATCCTCGGCGAGTGCCGCGGCGAGGAGGCTTTCGACATGCTGCAGGCGATGAACACCGGCCATGAAGG
GTCGATGGCGACCATCCACGCCAACACGCCGCGCGATGCCATTTCGCGCCTCGAACAGATGCTGGGCATGACCGGCATGC
CGATGACGGTGCAGTCGATCCGCAGTCAGATCGCCAGCGCCATCGACATCATCGTGCAACTGACCCGGCTTTCCGACGGC
AAGCGCAAGGTGACGAGCGTTGCCGAGGTGACCGGCATGGAAGGCGACGTCATCCAGATGCAGGAGATATTCCGCTTCGT
GCGCACCGGCATGGACGCCGATGGCGGCATTCTCGGTTATTTCGAGGCGACCGGCATCCGGCCGCGCTTCCTGGAGGATC
TGCGCGCCATGGGCATCGAGTTTCCCGGACGGTATTTCGAACCCGGTCGGCCGCAGGAGTAG

Upstream 100 bases:

>100_bases
ATCTGAAGAAGATCATCCTGCCGGAGGAGGCGGTCGCGACCGGAGCCAAATCGAAGTCGCTGTTCGGCCTCGGCAAGGGC
TTCTTGAAAAGGAAGGCCGG

Downstream 100 bases:

>100_bases
CCAGGTGTTCGACGGGCTGAGCGCAATCTATGTCGTCTATGCCGGAGCAGCACTTACCGGCATCATGATCGCCGAGGCCT
GCTATCTCCTCTATGCCGGC

Product: secretory protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MTSRFSTLQNRDARPQRSAESTPVAHHAVVIPTSRKALPAKPDVAPAKNANKVLDARVRIHRMLLEEINLVALERLPKDE
MRRQVHDFVSEKTRQERMAINTAELEALVDDIVDEMVGLGPLEPLLKDPEINDILINGHQNCFIEKKGKLQQVHIPFKDE
AHLLRIINKIVAAVGRRVDESQPMVDARMLDGSRFNAAIRPVGVDGPLVSIRKFSKNKLGLHKLVEFGAITQNMAEVLAA
AVHARKTTIISGGTGTGKTTMLNALSAFIPEDERLITIEDAAELQLQQPHVARMETRPANIEGHGELKQRDLVKNALRMR
PDRVILGECRGEEAFDMLQAMNTGHEGSMATIHANTPRDAISRLEQMLGMTGMPMTVQSIRSQIASAIDIIVQLTRLSDG
KRKVTSVAEVTGMEGDVIQMQEIFRFVRTGMDADGGILGYFEATGIRPRFLEDLRAMGIEFPGRYFEPGRPQE

Sequences:

>Translated_473_residues
MTSRFSTLQNRDARPQRSAESTPVAHHAVVIPTSRKALPAKPDVAPAKNANKVLDARVRIHRMLLEEINLVALERLPKDE
MRRQVHDFVSEKTRQERMAINTAELEALVDDIVDEMVGLGPLEPLLKDPEINDILINGHQNCFIEKKGKLQQVHIPFKDE
AHLLRIINKIVAAVGRRVDESQPMVDARMLDGSRFNAAIRPVGVDGPLVSIRKFSKNKLGLHKLVEFGAITQNMAEVLAA
AVHARKTTIISGGTGTGKTTMLNALSAFIPEDERLITIEDAAELQLQQPHVARMETRPANIEGHGELKQRDLVKNALRMR
PDRVILGECRGEEAFDMLQAMNTGHEGSMATIHANTPRDAISRLEQMLGMTGMPMTVQSIRSQIASAIDIIVQLTRLSDG
KRKVTSVAEVTGMEGDVIQMQEIFRFVRTGMDADGGILGYFEATGIRPRFLEDLRAMGIEFPGRYFEPGRPQE
>Mature_472_residues
TSRFSTLQNRDARPQRSAESTPVAHHAVVIPTSRKALPAKPDVAPAKNANKVLDARVRIHRMLLEEINLVALERLPKDEM
RRQVHDFVSEKTRQERMAINTAELEALVDDIVDEMVGLGPLEPLLKDPEINDILINGHQNCFIEKKGKLQQVHIPFKDEA
HLLRIINKIVAAVGRRVDESQPMVDARMLDGSRFNAAIRPVGVDGPLVSIRKFSKNKLGLHKLVEFGAITQNMAEVLAAA
VHARKTTIISGGTGTGKTTMLNALSAFIPEDERLITIEDAAELQLQQPHVARMETRPANIEGHGELKQRDLVKNALRMRP
DRVILGECRGEEAFDMLQAMNTGHEGSMATIHANTPRDAISRLEQMLGMTGMPMTVQSIRSQIASAIDIIVQLTRLSDGK
RKVTSVAEVTGMEGDVIQMQEIFRFVRTGMDADGGILGYFEATGIRPRFLEDLRAMGIEFPGRYFEPGRPQE

Specific function: Involved In A General Secretion Pathway (Gsp) For The Export Of Proteins (By Similarity). [C]

COG id: COG4962

COG function: function code U; Flp pilus assembly protein, ATPase CpaF

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022399
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 52562; Mature: 52430

Theoretical pI: Translated: 7.48; Mature: 7.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTSRFSTLQNRDARPQRSAESTPVAHHAVVIPTSRKALPAKPDVAPAKNANKVLDARVRI
CCCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
HRMLLEEINLVALERLPKDEMRRQVHDFVSEKTRQERMAINTAELEALVDDIVDEMVGLG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLEPLLKDPEINDILINGHQNCFIEKKGKLQQVHIPFKDEAHLLRIINKIVAAVGRRVDE
CCCHHHCCCCCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
SQPMVDARMLDGSRFNAAIRPVGVDGPLVSIRKFSKNKLGLHKLVEFGAITQNMAEVLAA
CCCCHHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AVHARKTTIISGGTGTGKTTMLNALSAFIPEDERLITIEDAAELQLQQPHVARMETRPAN
HHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHEECCCCHHHHCCCCCC
IEGHGELKQRDLVKNALRMRPDRVILGECRGEEAFDMLQAMNTGHEGSMATIHANTPRDA
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHH
ISRLEQMLGMTGMPMTVQSIRSQIASAIDIIVQLTRLSDGKRKVTSVAEVTGMEGDVIQM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
QEIFRFVRTGMDADGGILGYFEATGIRPRFLEDLRAMGIEFPGRYFEPGRPQE
HHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TSRFSTLQNRDARPQRSAESTPVAHHAVVIPTSRKALPAKPDVAPAKNANKVLDARVRI
CCHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHH
HRMLLEEINLVALERLPKDEMRRQVHDFVSEKTRQERMAINTAELEALVDDIVDEMVGLG
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PLEPLLKDPEINDILINGHQNCFIEKKGKLQQVHIPFKDEAHLLRIINKIVAAVGRRVDE
CCCHHHCCCCCCEEEEECCCCEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCC
SQPMVDARMLDGSRFNAAIRPVGVDGPLVSIRKFSKNKLGLHKLVEFGAITQNMAEVLAA
CCCCHHHHHCCCCCCCCEEEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHH
AVHARKTTIISGGTGTGKTTMLNALSAFIPEDERLITIEDAAELQLQQPHVARMETRPAN
HHHHHHEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEECCHHHHEECCCCHHHHCCCCCC
IEGHGELKQRDLVKNALRMRPDRVILGECRGEEAFDMLQAMNTGHEGSMATIHANTPRDA
CCCCCCHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCCEEEEECCCCHHH
ISRLEQMLGMTGMPMTVQSIRSQIASAIDIIVQLTRLSDGKRKVTSVAEVTGMEGDVIQM
HHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHH
QEIFRFVRTGMDADGGILGYFEATGIRPRFLEDLRAMGIEFPGRYFEPGRPQE
HHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12218036; 9634230 [H]