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The map label for this gene is ycdT [H]

Identifier: 13471023

GI number: 13471023

Start: 712240

End: 713415

Strand: Reverse

Name: ycdT [H]

Synonym: mll0886

Alternate gene names: 13471023

Gene position: 713415-712240 (Counterclockwise)

Preceding gene: 13471024

Following gene: 13471019

Centisome position: 10.14

GC content: 64.71

Gene sequence:

>1176_bases
ATGCTGGATTTCAGTTCGCTCCTGCTCGCGGCCGCGCTGTCGGGCACATGCCTGGCCGTCACCATGTTTGCCATCTGGCT
CACCGCGCCGCGGGCGCGTTTCGTGCTGACGGTGGCGTGCGGCATCCTCGTGCTTGTCGCGCATGTGATCCTGTTCTGGC
GTTACACCAAGGAGCCCGATCCGCTGCTTTGCCAGATCGCGCTGGCGCTGCTCAGCCTGGGCTTCCTGATTATCTGCCTT
TCGGCCATGCAGTATCTCGGCGTACCGGATCATAGACGAGTGATCGTGCCGACGCTTGCCGCCATGGCAGTCTGCGCCGC
CTTTACCTTCCTCGGCCTTGACGGTGTCGGCTTCATCGTCACCTACGCGACGGTGACCGTGCTGCTGTCGACGATCGGGG
CCATGTTCTGGGTTAATGGCAGCCACGACCGCCGGATCCTGCTGGTGGTTTCGTTCCTGAGTGGCACCTGCGCGGTGTCG
TTTGCCCTTTGCGGCGTGGTTCTGATGGCGAAGGGGCAATGGGCGCTCGGGGCGGCGCCTGACAACTGGGCCGAACGTCT
GAATTCCGTCGTGGCGGTGGCCTGCATGACGGGTCTGGGCGCCCTGACGCTCTCGCTGCACCATTTGCAGGCGCAGATCG
AACTGAAGGCCGAGACGATGACCGATCCATTGACCGGGCTGATGAACCGTCGTGGGTTGATGTCCCTCCACGGCGAGCGG
CCCTTCGGTCCGTTCATGGCGGTCGTCATGTTCGATCTCGATCACTTCAAGAGGACGAACGACATCTACGGTCACCCGGT
CGGGGACCAGGTGCTGTGCCGCTTCGCCGCCGTCATCCGGAAATATGCCAGGACCGGCGTCGACGCCTTTCGCCTGGGCG
GCGAGGAGTTCGCCATCGTCATGTCGCGGATGACGGAAGAGCGCGCCCATGATCTCGCCAGCAAGATCGGCGTCGCCTTC
GGCACCGAGATCGTTCCTACCCCGCTCGGCCCATTGCGCAGCACGGTCAGCGGCGGCATCGGCTTTGGCGGCGCTGACGG
CAGCAGCCTCGACGAGGTCCTGGCCGAGGCGGACGCCGCGCTCTATGCGGCCAAGCGCGCCGGCCGAAACTGCGTTGTCA
GCCGCAAAAGAATGGGCAAGGCCGAACCGGTCAAGCCGGCCTTGCGCTCTGCGTAG

Upstream 100 bases:

>100_bases
CGCAGCTTGGGTCGAAAGACCTGCTGAAAGCCATGTTCGAGGGCTGCCGGTGACCTAGAATGCGGCGTAACCGCCGACGC
TGGAGAGGCCCCCGCCCGAT

Downstream 100 bases:

>100_bases
GCCGAGAGGCTGCTATTCGGCTGCTCCCAGATAATCCGACAAAGGCGGGCAGGAGCAGACCAGATTGCGGTCGCCGGCGA
CATTGTCGATGCGCGACACC

Product: hypothetical protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MLDFSSLLLAAALSGTCLAVTMFAIWLTAPRARFVLTVACGILVLVAHVILFWRYTKEPDPLLCQIALALLSLGFLIICL
SAMQYLGVPDHRRVIVPTLAAMAVCAAFTFLGLDGVGFIVTYATVTVLLSTIGAMFWVNGSHDRRILLVVSFLSGTCAVS
FALCGVVLMAKGQWALGAAPDNWAERLNSVVAVACMTGLGALTLSLHHLQAQIELKAETMTDPLTGLMNRRGLMSLHGER
PFGPFMAVVMFDLDHFKRTNDIYGHPVGDQVLCRFAAVIRKYARTGVDAFRLGGEEFAIVMSRMTEERAHDLASKIGVAF
GTEIVPTPLGPLRSTVSGGIGFGGADGSSLDEVLAEADAALYAAKRAGRNCVVSRKRMGKAEPVKPALRSA

Sequences:

>Translated_391_residues
MLDFSSLLLAAALSGTCLAVTMFAIWLTAPRARFVLTVACGILVLVAHVILFWRYTKEPDPLLCQIALALLSLGFLIICL
SAMQYLGVPDHRRVIVPTLAAMAVCAAFTFLGLDGVGFIVTYATVTVLLSTIGAMFWVNGSHDRRILLVVSFLSGTCAVS
FALCGVVLMAKGQWALGAAPDNWAERLNSVVAVACMTGLGALTLSLHHLQAQIELKAETMTDPLTGLMNRRGLMSLHGER
PFGPFMAVVMFDLDHFKRTNDIYGHPVGDQVLCRFAAVIRKYARTGVDAFRLGGEEFAIVMSRMTEERAHDLASKIGVAF
GTEIVPTPLGPLRSTVSGGIGFGGADGSSLDEVLAEADAALYAAKRAGRNCVVSRKRMGKAEPVKPALRSA
>Mature_391_residues
MLDFSSLLLAAALSGTCLAVTMFAIWLTAPRARFVLTVACGILVLVAHVILFWRYTKEPDPLLCQIALALLSLGFLIICL
SAMQYLGVPDHRRVIVPTLAAMAVCAAFTFLGLDGVGFIVTYATVTVLLSTIGAMFWVNGSHDRRILLVVSFLSGTCAVS
FALCGVVLMAKGQWALGAAPDNWAERLNSVVAVACMTGLGALTLSLHHLQAQIELKAETMTDPLTGLMNRRGLMSLHGER
PFGPFMAVVMFDLDHFKRTNDIYGHPVGDQVLCRFAAVIRKYARTGVDAFRLGGEEFAIVMSRMTEERAHDLASKIGVAF
GTEIVPTPLGPLRSTVSGGIGFGGADGSSLDEVLAEADAALYAAKRAGRNCVVSRKRMGKAEPVKPALRSA

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=167, Percent_Identity=35.9281437125749, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI87081881, Length=173, Percent_Identity=36.4161849710983, Blast_Score=92, Evalue=8e-20,
Organism=Escherichia coli, GI1786584, Length=231, Percent_Identity=29.8701298701299, Blast_Score=85, Evalue=7e-18,
Organism=Escherichia coli, GI1787802, Length=245, Percent_Identity=28.1632653061224, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI145693134, Length=172, Percent_Identity=31.9767441860465, Blast_Score=78, Evalue=1e-15,
Organism=Escherichia coli, GI87082007, Length=171, Percent_Identity=33.3333333333333, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787816, Length=154, Percent_Identity=32.4675324675325, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1788085, Length=187, Percent_Identity=27.807486631016, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=31.547619047619, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788956, Length=169, Percent_Identity=31.3609467455621, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1788381, Length=144, Percent_Identity=31.9444444444444, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 41818; Mature: 41818

Theoretical pI: Translated: 8.42; Mature: 8.42

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDFSSLLLAAALSGTCLAVTMFAIWLTAPRARFVLTVACGILVLVAHVILFWRYTKEPD
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLLCQIALALLSLGFLIICLSAMQYLGVPDHRRVIVPTLAAMAVCAAFTFLGLDGVGFIV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHH
TYATVTVLLSTIGAMFWVNGSHDRRILLVVSFLSGTCAVSFALCGVVLMAKGQWALGAAP
HHHHHHHHHHHHHHHEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
DNWAERLNSVVAVACMTGLGALTLSLHHLQAQIELKAETMTDPLTGLMNRRGLMSLHGER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCHHHHHHHCCCCHHCCCCC
PFGPFMAVVMFDLDHFKRTNDIYGHPVGDQVLCRFAAVIRKYARTGVDAFRLGGEEFAIV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
MSRMTEERAHDLASKIGVAFGTEIVPTPLGPLRSTVSGGIGFGGADGSSLDEVLAEADAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LYAAKRAGRNCVVSRKRMGKAEPVKPALRSA
HHHHHHCCCCHHHHHHHCCCCCCCCHHHCCC
>Mature Secondary Structure
MLDFSSLLLAAALSGTCLAVTMFAIWLTAPRARFVLTVACGILVLVAHVILFWRYTKEPD
CCCHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
PLLCQIALALLSLGFLIICLSAMQYLGVPDHRRVIVPTLAAMAVCAAFTFLGLDGVGFIV
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCHHHHHH
TYATVTVLLSTIGAMFWVNGSHDRRILLVVSFLSGTCAVSFALCGVVLMAKGQWALGAAP
HHHHHHHHHHHHHHHEEECCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHCCCEEECCCC
DNWAERLNSVVAVACMTGLGALTLSLHHLQAQIELKAETMTDPLTGLMNRRGLMSLHGER
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEHHHHHCCHHHHHHHCCCCHHCCCCC
PFGPFMAVVMFDLDHFKRTNDIYGHPVGDQVLCRFAAVIRKYARTGVDAFRLGGEEFAIV
CCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCCHHHHHH
MSRMTEERAHDLASKIGVAFGTEIVPTPLGPLRSTVSGGIGFGGADGSSLDEVLAEADAA
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHH
LYAAKRAGRNCVVSRKRMGKAEPVKPALRSA
HHHHHHCCCCHHHHHHHCCCCCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]