Click here to switch to the map view.

The map label for this gene is gcvH

Identifier: 13471021

GI number: 13471021

Start: 709022

End: 709399

Strand: Direct

Name: gcvH

Synonym: mlr0884

Alternate gene names: 13471021

Gene position: 709022-709399 (Clockwise)

Preceding gene: 13471020

Following gene: 13471022

Centisome position: 10.08

GC content: 63.49

Gene sequence:

>378_bases
ATGAACCTCATGGCAAAGACCTATTTCACATCAGATCACGAATGGCTCCGCGTTGAGGGCGGCATCGCCACCGTCGGCAT
CACCGACTACGCGCAGGAGCAGCTCGGCGACCTCGTCTTCGTCGAACTGCCGGAAACGGGCAAGAAGCTGACCAAGGGCG
ACACCGCCGTCGTGGTCGAATCCGTCAAGGCGGCTTCCGACGTTTACGCGCCGGTCGACGGCGAGATCACCGAGGCCAAC
GGCACGCTGTCTTCCGACCCGTCGCTGGTCAACTCGGCGGCAACCGGCGCCGGCTGGCTGTGGAAGATGAGGCTTGCCGA
CGAAAGCCAGCTCGCCGGCCTCATGGATGAGGCCGCCTACAAAGCCCATATCGCCTGA

Upstream 100 bases:

>100_bases
TGGCAAAGCCCGGCACACGGGTGTTCGCCGAGGTACGCGGCACGAAGATCCCCGTCGATATCAGTTCCCTGCCCTTCACC
CCTCATCGCTACCGCAAAGG

Downstream 100 bases:

>100_bases
AATCAGGACTAGAGATGATGACCACAGCACTTACCCCCTTCTCGGCCCGTCATATCGGTCCTGGCGTGAACGATGTCAGA
GCCATGCTTGCGGTGATCGG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 125

Protein sequence:

>125_residues
MNLMAKTYFTSDHEWLRVEGGIATVGITDYAQEQLGDLVFVELPETGKKLTKGDTAVVVESVKAASDVYAPVDGEITEAN
GTLSSDPSLVNSAATGAGWLWKMRLADESQLAGLMDEAAYKAHIA

Sequences:

>Translated_125_residues
MNLMAKTYFTSDHEWLRVEGGIATVGITDYAQEQLGDLVFVELPETGKKLTKGDTAVVVESVKAASDVYAPVDGEITEAN
GTLSSDPSLVNSAATGAGWLWKMRLADESQLAGLMDEAAYKAHIA
>Mature_125_residues
MNLMAKTYFTSDHEWLRVEGGIATVGITDYAQEQLGDLVFVELPETGKKLTKGDTAVVVESVKAASDVYAPVDGEITEAN
GTLSSDPSLVNSAATGAGWLWKMRLADESQLAGLMDEAAYKAHIA

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain

Homologues:

Organism=Homo sapiens, GI89057342, Length=122, Percent_Identity=50, Blast_Score=129, Evalue=8e-31,
Organism=Homo sapiens, GI49574537, Length=122, Percent_Identity=50, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1789271, Length=122, Percent_Identity=53.2786885245902, Blast_Score=132, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17507493, Length=112, Percent_Identity=48.2142857142857, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17551294, Length=112, Percent_Identity=49.1071428571429, Blast_Score=123, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6319272, Length=117, Percent_Identity=43.5897435897436, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI17865652, Length=126, Percent_Identity=46.8253968253968, Blast_Score=115, Evalue=6e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCSH_RHILO (Q98LT7)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102590.1
- ProteinModelPortal:   Q98LT7
- SMR:   Q98LT7
- GeneID:   1225253
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0884
- NMPDR:   fig|266835.1.peg.695
- HOGENOM:   HBG693789
- OMA:   TSDHEWL
- ProtClustDB:   PRK01202
- GO:   GO:0005739
- HAMAP:   MF_00272
- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053
- PANTHER:   PTHR11715
- TIGRFAMs:   TIGR00527

Pfam domain/function: PF01597 GCV_H; SSF51230 Hybrid_motif

EC number: NA

Molecular weight: Translated: 13322; Mature: 13322

Theoretical pI: Translated: 4.17; Mature: 4.17

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLMAKTYFTSDHEWLRVEGGIATVGITDYAQEQLGDLVFVELPETGKKLTKGDTAVVVE
CCCCCCEEECCCCCEEEEECCEEEEECCHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEH
SVKAASDVYAPVDGEITEANGTLSSDPSLVNSAATGAGWLWKMRLADESQLAGLMDEAAY
HHHHHCCCCCCCCCEEECCCCCCCCCCCHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHH
KAHIA
HCCCC
>Mature Secondary Structure
MNLMAKTYFTSDHEWLRVEGGIATVGITDYAQEQLGDLVFVELPETGKKLTKGDTAVVVE
CCCCCCEEECCCCCEEEEECCEEEEECCHHHHHHHCCEEEEECCCCCCCCCCCCCEEEEH
SVKAASDVYAPVDGEITEANGTLSSDPSLVNSAATGAGWLWKMRLADESQLAGLMDEAAY
HHHHHCCCCCCCCCEEECCCCCCCCCCCHHHCCCCCCCEEEEEEECCCHHHHHHHHHHHH
KAHIA
HCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968