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The map label for this gene is nadE [H]

Identifier: 13470971

GI number: 13470971

Start: 648643

End: 650319

Strand: Reverse

Name: nadE [H]

Synonym: mll0818

Alternate gene names: 13470971

Gene position: 650319-648643 (Counterclockwise)

Preceding gene: 13470972

Following gene: 13470970

Centisome position: 9.24

GC content: 62.49

Gene sequence:

>1677_bases
ATGACCAAGAAGCCCGACATACTGCGCATCGCGATCGCCCAGCTCAATCCGACCGTCGGCGATGTCGCCGGCAATCTCGC
CAAGGCCCGCGAGGCGAGGGCGGATGCCGCGCGCCAGGGCGCCGATCTCGTGCTCTACACCGAGCTTTTCCTTGCCGGTT
ACCCACCTGAAGATCTGGTGCTGAAGCCGGCCTTCCTGAAGGCTTGCGAAAAGGCGGCGCAGGAGTTTGCCGCCGATACA
TCGGATGGCGGCCCCGGCGTGATCATCGGCACGCCGCTGAAGCGCAAGAGCGGCACGCACAATTCGATCATCGTCGCTGA
TGGCGGCAAGATCATCGCCGAACGCTACAAGCTCGACCTGCCGAATTATGGCGAGTTCGACGAGAAGCGCGTGTTCCAGG
CTGGACCCGAGATCCAGGGACCTGTCAATTTCCGTGGCGTGCGCATCGGAATTCCGATTTGCGAGGACATCTGGGGCGAT
GTCGGCATCTGCGAATCGCTGGCGGAAAGCGGGGCGGAAATCCTGCTGGTGCCGAACGGCTCGCCTTACTATCGCGCCAA
AATCGATGTCCGCTACCAGGTCGTCATTCGCCAGATCATCGAATGCGGGCTGCCGATCACCTATGCCAATCAGCTTGGCG
GCCAGGATGAGCTGATCTTCGACGGCGCGTCGTTTGCCATCGGCGCCGACAAGACATTGGCTTTCCAGATGAGTCAGTTC
GAGGAAGCGGTCGACGTCGTCACATGGAAGCGCGGGGAGGACGGCTGGGTCTGTTCGGAAGGGCCGATGTCGAAGATCCC
GGAGCGGGAGGAGGCCGACTATCGCGCCTGCATGCTGGGCCTGCGCGACTACGTCAACAAGAACGGCTTCAAGAATGTGG
TGCTCGGCCTGTCCGGCGGCATCGATTCGGCGATCTGTGCCGCCCTTGCCGTCGACGCGCTCGGCGAAGAGCGGCTGCGC
GCGGTGATGATGCCCTATCGCTACACCTCGAAGGATTCGCTCAAGGACGCCGAGGATTGCGCCCGGGCGCTCGGCTGCCG
CTATGACATCGTGCCGATCTTCGAGCCGGTCGAAGGTTTCCTGCATGCGCTGACGCAGCTCTTCGAGGGCACCAAGGAAG
GCATCACCGAGGAAAACCTGCAGAGCCGCGCGCGCGGCACCATCCTGATGGCGATCTCCAACAAGTTCGGCTCGATGGTG
GTTACGACAGGCAACAAGAGCGAAATGTCGGTTGGCTATGCGACGCTCTATGGCGACATGAATGGCGGCTTCAACCCGAT
CAAGGATCTCTACAAGATGCAGGTCTACGCGCTGTCGCGCTGGCGCAACAGCCATGTGCCGCCGGGCGCGCTCGGACCCT
CGGGCGAGGTCATTCCGAAGAACATCATCGACAAGGCGCCGTCGGCGGAATTGCGCGAGAACCAGACGGACCAGGATTCG
CTGCCGCCCTATCCGGTGCTGGACGACATCCTCGAATGCCTGGTCGAGAACGAGATGGGCGTCGACGACATTGTCGCGCG
CGGCCATGACCGGGCGACGGTGACGCGCATCGAACACCTGCTCTACATCGCCGAATACAAGCGCCGGCAGGCGGCACCAG
GGGTGAAGATCACCCGGAAGAATTTCGGCCGCGACCGCCGCTATCCCATCACCAACCGCTTCAGGGATCGTGGGTAA

Upstream 100 bases:

>100_bases
TCCCAACACGACTGATGACGAATGACGTCTGTCATTCGGTCGGAGTTGGTCCAAGCGAACGCCATTGCGAGGCGAATTTT
GCCGCGCTAAGTCAGCAGGC

Downstream 100 bases:

>100_bases
GCGCGTTCGTCCGCTGGGCGAAAATACGCGGTGCCGCGGGGCGAGGATACAGCATGCCGGACTGTGAGATCAGCTTCGAC
CTCGCGCGGATCGACTTTCG

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 558; Mature: 557

Protein sequence:

>558_residues
MTKKPDILRIAIAQLNPTVGDVAGNLAKAREARADAARQGADLVLYTELFLAGYPPEDLVLKPAFLKACEKAAQEFAADT
SDGGPGVIIGTPLKRKSGTHNSIIVADGGKIIAERYKLDLPNYGEFDEKRVFQAGPEIQGPVNFRGVRIGIPICEDIWGD
VGICESLAESGAEILLVPNGSPYYRAKIDVRYQVVIRQIIECGLPITYANQLGGQDELIFDGASFAIGADKTLAFQMSQF
EEAVDVVTWKRGEDGWVCSEGPMSKIPEREEADYRACMLGLRDYVNKNGFKNVVLGLSGGIDSAICAALAVDALGEERLR
AVMMPYRYTSKDSLKDAEDCARALGCRYDIVPIFEPVEGFLHALTQLFEGTKEGITEENLQSRARGTILMAISNKFGSMV
VTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALSRWRNSHVPPGALGPSGEVIPKNIIDKAPSAELRENQTDQDS
LPPYPVLDDILECLVENEMGVDDIVARGHDRATVTRIEHLLYIAEYKRRQAAPGVKITRKNFGRDRRYPITNRFRDRG

Sequences:

>Translated_558_residues
MTKKPDILRIAIAQLNPTVGDVAGNLAKAREARADAARQGADLVLYTELFLAGYPPEDLVLKPAFLKACEKAAQEFAADT
SDGGPGVIIGTPLKRKSGTHNSIIVADGGKIIAERYKLDLPNYGEFDEKRVFQAGPEIQGPVNFRGVRIGIPICEDIWGD
VGICESLAESGAEILLVPNGSPYYRAKIDVRYQVVIRQIIECGLPITYANQLGGQDELIFDGASFAIGADKTLAFQMSQF
EEAVDVVTWKRGEDGWVCSEGPMSKIPEREEADYRACMLGLRDYVNKNGFKNVVLGLSGGIDSAICAALAVDALGEERLR
AVMMPYRYTSKDSLKDAEDCARALGCRYDIVPIFEPVEGFLHALTQLFEGTKEGITEENLQSRARGTILMAISNKFGSMV
VTTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALSRWRNSHVPPGALGPSGEVIPKNIIDKAPSAELRENQTDQDS
LPPYPVLDDILECLVENEMGVDDIVARGHDRATVTRIEHLLYIAEYKRRQAAPGVKITRKNFGRDRRYPITNRFRDRG
>Mature_557_residues
TKKPDILRIAIAQLNPTVGDVAGNLAKAREARADAARQGADLVLYTELFLAGYPPEDLVLKPAFLKACEKAAQEFAADTS
DGGPGVIIGTPLKRKSGTHNSIIVADGGKIIAERYKLDLPNYGEFDEKRVFQAGPEIQGPVNFRGVRIGIPICEDIWGDV
GICESLAESGAEILLVPNGSPYYRAKIDVRYQVVIRQIIECGLPITYANQLGGQDELIFDGASFAIGADKTLAFQMSQFE
EAVDVVTWKRGEDGWVCSEGPMSKIPEREEADYRACMLGLRDYVNKNGFKNVVLGLSGGIDSAICAALAVDALGEERLRA
VMMPYRYTSKDSLKDAEDCARALGCRYDIVPIFEPVEGFLHALTQLFEGTKEGITEENLQSRARGTILMAISNKFGSMVV
TTGNKSEMSVGYATLYGDMNGGFNPIKDLYKMQVYALSRWRNSHVPPGALGPSGEVIPKNIIDKAPSAELRENQTDQDSL
PPYPVLDDILECLVENEMGVDDIVARGHDRATVTRIEHLLYIAEYKRRQAAPGVKITRKNFGRDRRYPITNRFRDRG

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Saccharomyces cerevisiae, GI6321865, Length=627, Percent_Identity=23.6044657097289, Blast_Score=86, Evalue=1e-17,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 61314; Mature: 61182

Theoretical pI: Translated: 5.08; Mature: 5.08

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKPDILRIAIAQLNPTVGDVAGNLAKAREARADAARQGADLVLYTELFLAGYPPEDLV
CCCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHCE
LKPAFLKACEKAAQEFAADTSDGGPGVIIGTPLKRKSGTHNSIIVADGGKIIAERYKLDL
ECHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCEEEHHHCCCC
PNYGEFDEKRVFQAGPEIQGPVNFRGVRIGIPICEDIWGDVGICESLAESGAEILLVPNG
CCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHCCCEEEEEECC
SPYYRAKIDVRYQVVIRQIIECGLPITYANQLGGQDELIFDGASFAIGADKTLAFQMSQF
CCEEEEEECHHHHHHHHHHHHCCCCEEEHHHCCCCCCEEECCCCEEECCCCHHHHHHHHH
EEAVDVVTWKRGEDGWVCSEGPMSKIPEREEADYRACMLGLRDYVNKNGFKNVVLGLSGG
HHHHHHHEEECCCCCEEECCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCC
IDSAICAALAVDALGEERLRAVMMPYRYTSKDSLKDAEDCARALGCRYDIVPIFEPVEGF
HHHHHHHHHHHHHHCHHHHHHHHCCCEECCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHH
LHALTQLFEGTKEGITEENLQSRARGTILMAISNKFGSMVVTTGNKSEMSVGYATLYGDM
HHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEECC
NGGFNPIKDLYKMQVYALSRWRNSHVPPGALGPSGEVIPKNIIDKAPSAELRENQTDQDS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCCCCCC
LPPYPVLDDILECLVENEMGVDDIVARGHDRATVTRIEHLLYIAEYKRRQAAPGVKITRK
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
NFGRDRRYPITNRFRDRG
HCCCCCCCCCHHHHCCCC
>Mature Secondary Structure 
TKKPDILRIAIAQLNPTVGDVAGNLAKAREARADAARQGADLVLYTELFLAGYPPEDLV
CCCCCEEEEEEHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEHHHHCCCCCHHCE
LKPAFLKACEKAAQEFAADTSDGGPGVIIGTPLKRKSGTHNSIIVADGGKIIAERYKLDL
ECHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCCCCCCCCEEEEECCCCEEEHHHCCCC
PNYGEFDEKRVFQAGPEIQGPVNFRGVRIGIPICEDIWGDVGICESLAESGAEILLVPNG
CCCCCCCHHHHHHCCCCCCCCCCEEEEEECCCHHHHHCCCHHHHHHHHHCCCEEEEEECC
SPYYRAKIDVRYQVVIRQIIECGLPITYANQLGGQDELIFDGASFAIGADKTLAFQMSQF
CCEEEEEECHHHHHHHHHHHHCCCCEEEHHHCCCCCCEEECCCCEEECCCCHHHHHHHHH
EEAVDVVTWKRGEDGWVCSEGPMSKIPEREEADYRACMLGLRDYVNKNGFKNVVLGLSGG
HHHHHHHEEECCCCCEEECCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEECCC
IDSAICAALAVDALGEERLRAVMMPYRYTSKDSLKDAEDCARALGCRYDIVPIFEPVEGF
HHHHHHHHHHHHHHCHHHHHHHHCCCEECCCCCCCCHHHHHHHHCCCCCEEEHHHHHHHH
LHALTQLFEGTKEGITEENLQSRARGTILMAISNKFGSMVVTTGNKSEMSVGYATLYGDM
HHHHHHHHHHHHHCCCHHHHHHHHCCEEEEEEECCCCCEEEEECCCCCCEEEEEEEEECC
NGGFNPIKDLYKMQVYALSRWRNSHVPPGALGPSGEVIPKNIIDKAPSAELRENQTDQDS
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHCCCCCCHHHCCCCCCCC
LPPYPVLDDILECLVENEMGVDDIVARGHDRATVTRIEHLLYIAEYKRRQAAPGVKITRK
CCCCHHHHHHHHHHHCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEEHH
NFGRDRRYPITNRFRDRG
HCCCCCCCCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]