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The map label for this gene is lipA

Identifier: 13470626

GI number: 13470626

Start: 277610

End: 278575

Strand: Direct

Name: lipA

Synonym: mlr0392

Alternate gene names: 13470626

Gene position: 277610-278575 (Clockwise)

Preceding gene: 13470625

Following gene: 13470627

Centisome position: 3.95

GC content: 62.84

Gene sequence:

>966_bases
ATGGTTACTGTCCTCGACGCGATCGCCAACGCACCACGGTTGCGGCACCCCGAGAAGGCGCACAAGCCCGACCAGGAGGT
GTTGCGCAAGCCGGACTGGATCCGCGTCAAGGCGCCGGTCTCGAAGGGCTATGCCGAGACGCGCGAGATCGTCAAATCCC
ACAAGCTGGTGACGGTCTGCGAAGAGGCCGGTTGCCCCAACATCGGCGAGTGCTGGGAAAAGAAGCACGCCACCTTCATG
ATCATGGGCGAGATCTGCACGCGCGCTTGCGCCTTCTGCAATGTCGCCACCGGCATTCCGACCGCGCTGGACCCCGATGA
GCCGGCCCGCGTCGCGCATGCCGTCAAGCAGATGGGGCTCAGCCACGTCGTCATCACCTCGGTCGACCGCGACGATCTTG
CCGATGGCGGTGCGCAGCATTTTGCCGATGTCATCCGGGCGATCCGCGCGGCAACGCCGTCGACCACGATCGAAATCCTG
ACGCCCGATTTCCTGCGCAAGGACGGCGCGCTTGAGATCGTCGTGGCGGCCAGGCCCGACGTCTTCAACCACAATCTCGA
AACCGTGCCGTCGAACTATCTGAAGGTCCGGCCGGGCGCCCGCTATTTCCACTCGATCCGGCTGTTGCAGCGGGTCAAGG
AACTCGATCCGTCGATCTTCACCAAATCCGGCATCATGGTTGGCCTGGGCGAGGAGCGAAATGAAATCCTGCAGCTGATG
GACGATCTGCGCTCGGCCAATGTCGACTTCATGACCATCGGCCAATATCTGCAGCCTTCGAAAAAGCACCATCCGGTGAT
CCGCTTCGTGACGCCCGAAGAGTTCAAGTCCTTCGAGACGATCGGCCGGACCAAGGGTTTCCTGCTTGTGGCATCGAGCC
CGCTGACGCGTTCATCGCACCATGCCGGCGACGATTTCGCCCGGCTGCGCGCGGCGCGGGAAGCGCAGCTCCAGAAATCG
GTCTAG

Upstream 100 bases:

>100_bases
GGCATGCGCCGCTGCAGCCTTTTTCTTTGTGCCGAAAAGTCCTAGATGACGATAAACTGACGATCGCCGAGGGGCGGTCA
CGAGAAAAGCCAGGGTCCAG

Downstream 100 bases:

>100_bases
CGCTGGTTGAATGCCGAAATTCGAAGCCACCCGCCGCGTCGCCCATACGCCACAGCAGATGTTCGCGCTGGTTGCCGATG
TCGAGGCCTATCCGCAGTTC

Product: lipoyl synthase

Products: NA

Alternate protein names: Lip-syn; LS; Lipoate synthase; Lipoic acid synthase; Sulfur insertion protein lipA

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MVTVLDAIANAPRLRHPEKAHKPDQEVLRKPDWIRVKAPVSKGYAETREIVKSHKLVTVCEEAGCPNIGECWEKKHATFM
IMGEICTRACAFCNVATGIPTALDPDEPARVAHAVKQMGLSHVVITSVDRDDLADGGAQHFADVIRAIRAATPSTTIEIL
TPDFLRKDGALEIVVAARPDVFNHNLETVPSNYLKVRPGARYFHSIRLLQRVKELDPSIFTKSGIMVGLGEERNEILQLM
DDLRSANVDFMTIGQYLQPSKKHHPVIRFVTPEEFKSFETIGRTKGFLLVASSPLTRSSHHAGDDFARLRAAREAQLQKS
V

Sequences:

>Translated_321_residues
MVTVLDAIANAPRLRHPEKAHKPDQEVLRKPDWIRVKAPVSKGYAETREIVKSHKLVTVCEEAGCPNIGECWEKKHATFM
IMGEICTRACAFCNVATGIPTALDPDEPARVAHAVKQMGLSHVVITSVDRDDLADGGAQHFADVIRAIRAATPSTTIEIL
TPDFLRKDGALEIVVAARPDVFNHNLETVPSNYLKVRPGARYFHSIRLLQRVKELDPSIFTKSGIMVGLGEERNEILQLM
DDLRSANVDFMTIGQYLQPSKKHHPVIRFVTPEEFKSFETIGRTKGFLLVASSPLTRSSHHAGDDFARLRAAREAQLQKS
V
>Mature_321_residues
MVTVLDAIANAPRLRHPEKAHKPDQEVLRKPDWIRVKAPVSKGYAETREIVKSHKLVTVCEEAGCPNIGECWEKKHATFM
IMGEICTRACAFCNVATGIPTALDPDEPARVAHAVKQMGLSHVVITSVDRDDLADGGAQHFADVIRAIRAATPSTTIEIL
TPDFLRKDGALEIVVAARPDVFNHNLETVPSNYLKVRPGARYFHSIRLLQRVKELDPSIFTKSGIMVGLGEERNEILQLM
DDLRSANVDFMTIGQYLQPSKKHHPVIRFVTPEEFKSFETIGRTKGFLLVASSPLTRSSHHAGDDFARLRAAREAQLQKS
V

Specific function: Catalyzes the radical-mediated insertion of two sulfur atoms into the C-6 and C-8 positions of the octanoyl moiety bound to the lipoyl domains of lipoate-dependent enzymes, thereby converting the octanoylated domains into lipoylated derivatives

COG id: COG0320

COG function: function code H; Lipoate synthase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Lipoyl synthase family

Homologues:

Organism=Homo sapiens, GI37577166, Length=295, Percent_Identity=43.728813559322, Blast_Score=261, Evalue=5e-70,
Organism=Homo sapiens, GI37577164, Length=248, Percent_Identity=43.9516129032258, Blast_Score=221, Evalue=6e-58,
Organism=Escherichia coli, GI1786846, Length=280, Percent_Identity=52.8571428571429, Blast_Score=299, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI32564533, Length=258, Percent_Identity=45.3488372093023, Blast_Score=235, Evalue=3e-62,
Organism=Saccharomyces cerevisiae, GI6324770, Length=296, Percent_Identity=44.2567567567568, Blast_Score=256, Evalue=3e-69,
Organism=Drosophila melanogaster, GI221513272, Length=296, Percent_Identity=44.5945945945946, Blast_Score=262, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPA_RHILO (Q98MY2)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102195.1
- ProteinModelPortal:   Q98MY2
- GeneID:   1224858
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0392
- NMPDR:   fig|266835.1.peg.300
- HOGENOM:   HBG284542
- OMA:   TTIEVLI
- ProtClustDB:   PRK05481
- BRENDA:   2.8.1.8
- GO:   GO:0005737
- HAMAP:   MF_00206
- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR003698
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- PIRSF:   PIRSF005963
- SMART:   SM00729
- TIGRFAMs:   TIGR00510

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.8.1.8

Molecular weight: Translated: 35684; Mature: 35684

Theoretical pI: Translated: 8.39; Mature: 8.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTVLDAIANAPRLRHPEKAHKPDQEVLRKPDWIRVKAPVSKGYAETREIVKSHKLVTVC
CEEHHHHHCCCCCCCCCHHHCCCCHHHHCCCCCEEEECCHHCCHHHHHHHHHHCCCEEEH
EEAGCPNIGECWEKKHATFMIMGEICTRACAFCNVATGIPTALDPDEPARVAHAVKQMGL
HHCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
SHVVITSVDRDDLADGGAQHFADVIRAIRAATPSTTIEILTPDFLRKDGALEIVVAARPD
CEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEEECCC
VFNHNLETVPSNYLKVRPGARYFHSIRLLQRVKELDPSIFTKSGIMVGLGEERNEILQLM
HHCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHHHHHH
DDLRSANVDFMTIGQYLQPSKKHHPVIRFVTPEEFKSFETIGRTKGFLLVASSPLTRSSH
HHHHHCCCCCEEHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCEEEEECCCCCCCCC
HAGDDFARLRAAREAQLQKSV
CCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVTVLDAIANAPRLRHPEKAHKPDQEVLRKPDWIRVKAPVSKGYAETREIVKSHKLVTVC
CEEHHHHHCCCCCCCCCHHHCCCCHHHHCCCCCEEEECCHHCCHHHHHHHHHHCCCEEEH
EEAGCPNIGECWEKKHATFMIMGEICTRACAFCNVATGIPTALDPDEPARVAHAVKQMGL
HHCCCCCHHHHHHHHCCEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHCCC
SHVVITSVDRDDLADGGAQHFADVIRAIRAATPSTTIEILTPDFLRKDGALEIVVAARPD
CEEEEEECCHHHHCCCCHHHHHHHHHHHHHCCCCCEEEEECHHHHCCCCCEEEEEEECCC
VFNHNLETVPSNYLKVRPGARYFHSIRLLQRVKELDPSIFTKSGIMVGLGEERNEILQLM
HHCCCHHHCCCCCEEECCCHHHHHHHHHHHHHHHCCHHHEECCCEEEECCCCHHHHHHHH
DDLRSANVDFMTIGQYLQPSKKHHPVIRFVTPEEFKSFETIGRTKGFLLVASSPLTRSSH
HHHHHCCCCCEEHHHHHCCCCCCCCEEEEECHHHHHHHHHHCCCCCEEEEECCCCCCCCC
HAGDDFARLRAAREAQLQKSV
CCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11214968