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The map label for this gene is lpd [H]

Identifier: 13470624

GI number: 13470624

Start: 275670

End: 277115

Strand: Direct

Name: lpd [H]

Synonym: mlr0388

Alternate gene names: 13470624

Gene position: 275670-277115 (Clockwise)

Preceding gene: 13470623

Following gene: 13470625

Centisome position: 3.92

GC content: 62.79

Gene sequence:

>1446_bases
GTGGCTGAGAACTACGACGTCATCATCATCGGCTCGGGCCCCGGCGGCTATGTCACGGCGGTGCGTTCCGCCCAGCTCGG
CTTCAAGACGGCGATTGTCGAGCGCGAGCATCTCGGCGGCATCTGCCTCAACTGGGGCTGCATCCCGACCAAGGCGCTGC
TGCGTTCGGCCGAGATCATGCACTATTCCGACCATCTGAAGGACTATGGCCTGAAGCTCGATGGCAAGGTCAGCGTCGAC
ACGTCGGCCGTGGTCGACCGCTCGCGCAAAGTCTCGCTGCGGCTCAATGGCGGCGTTGCCTTCCTGATGAAGAAGAACAA
GGTCGACGTCATCTGGGGCGAAGCCAAGCTGTCGAAGCCCGGTGAGGTCGTCGTCTCCAAGACGGCCAAGACGCCGATGG
AGCCGCAGCCGCCGGTACCGAAGGGCGTCAAGGGCGAGGGCACCTATACCGCCAAGCACATCATCCTGGCGACCGGCGCG
CGGCCGCGCGCGCTGCCGGGCATCGAGCCGGACGGCAAGCTCATCTGGACCTATTTCGAGGCCATGGTGCCGAAGGAAAT
GCCGAAGTCGCTGCTGGTGATGGGCTCGGGCGCCATCGGCATCGAATTTGCCTCCTTCTACCGCACCATGGGCGCCGACG
TGACGGTGGTCGAACTGTTGCCGGCGGTGATGCCGGTCGAGGATGCCGAGGTCTCGAAATTCGCGCAAAAACAGTTCGAG
AAGCAAGGCATGAAGATCATTCTCGAAGCCAAGGTGACCAAGGTCGAGAAGGGCGCCAACTCGGTCACCGCGCATGTCGA
GATGAAGGACGGCAAGGTCGAGAAGATCACCGCCGACCGCATGATCTCGGCCGTCGGTGTCCAGGGCAATATCGAGAATC
TCGGCCTTGAAGCGCTCGGCGTGAAGACCGACCGCGGCTGCATTGTCGTCGACGGCTATGGCAAGACCAATGTACCAGGC
ATCTACGCCATCGGCGATGTCGCCGGCCCGCCCATGCTTGCCCACAAGGCCGAGCATGAGGGCGTCGTGTGCGTGGAAAA
GATCGCTGGTTTCCCCGGCGTGCACGCCATCGACAAGCTCAAGATCCCGGGCTGCACCTACTGCAATCCGCAGGTCGCCT
CCGTCGGCCTGACGGAAGCCAAGGCCAAGGCCGAAGGCAAGGATATCCGCGTCGGCCGCTTCCAGTTCGCGGCTAACGGC
AAGGCGATCGCGCTTGGCGAGGACCAGGGCTTCATCAAGACCATCTTCGACAAGAAGACCGGTCAGCTGCTCGGCGCCCA
TATGGTCGGCGCCGAGGTGACCGAACTGATCCAGGGCTTTGTCGTGGCGATGAACCTCGAGACGACCGAGGAAGAGCTGA
TGCACACCATCTTCCCGCATCCGACGCTGTCGGAGATGATGAAGGAAAGCGTGCTCGACGCCTATGGCCGCGCCTTGAAC
GCATGA

Upstream 100 bases:

>100_bases
GACGGCGTCCATCTCGATGCCGAAAACACGCGAAACATCGGCAAGGCGCTGACATCGGTCGTGCGCGTCATGCTGGAATT
GTGATTGAAGGAGAAAATCC

Downstream 100 bases:

>100_bases
TAGATTCGCAGCAGGAGACTCAGCCGCGAAAGCCGCCGGATCTCGGCGGCAAGGCAGTTCGTTTTCGCATGCATTTCTAC
GCGCGCAAGGAGAAGGCATA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of pyruvate and 2-oxoglutarate dehydrogenases complexes [H]

Number of amino acids: Translated: 481; Mature: 480

Protein sequence:

>481_residues
MAENYDVIIIGSGPGGYVTAVRSAQLGFKTAIVEREHLGGICLNWGCIPTKALLRSAEIMHYSDHLKDYGLKLDGKVSVD
TSAVVDRSRKVSLRLNGGVAFLMKKNKVDVIWGEAKLSKPGEVVVSKTAKTPMEPQPPVPKGVKGEGTYTAKHIILATGA
RPRALPGIEPDGKLIWTYFEAMVPKEMPKSLLVMGSGAIGIEFASFYRTMGADVTVVELLPAVMPVEDAEVSKFAQKQFE
KQGMKIILEAKVTKVEKGANSVTAHVEMKDGKVEKITADRMISAVGVQGNIENLGLEALGVKTDRGCIVVDGYGKTNVPG
IYAIGDVAGPPMLAHKAEHEGVVCVEKIAGFPGVHAIDKLKIPGCTYCNPQVASVGLTEAKAKAEGKDIRVGRFQFAANG
KAIALGEDQGFIKTIFDKKTGQLLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTLSEMMKESVLDAYGRALN
A

Sequences:

>Translated_481_residues
MAENYDVIIIGSGPGGYVTAVRSAQLGFKTAIVEREHLGGICLNWGCIPTKALLRSAEIMHYSDHLKDYGLKLDGKVSVD
TSAVVDRSRKVSLRLNGGVAFLMKKNKVDVIWGEAKLSKPGEVVVSKTAKTPMEPQPPVPKGVKGEGTYTAKHIILATGA
RPRALPGIEPDGKLIWTYFEAMVPKEMPKSLLVMGSGAIGIEFASFYRTMGADVTVVELLPAVMPVEDAEVSKFAQKQFE
KQGMKIILEAKVTKVEKGANSVTAHVEMKDGKVEKITADRMISAVGVQGNIENLGLEALGVKTDRGCIVVDGYGKTNVPG
IYAIGDVAGPPMLAHKAEHEGVVCVEKIAGFPGVHAIDKLKIPGCTYCNPQVASVGLTEAKAKAEGKDIRVGRFQFAANG
KAIALGEDQGFIKTIFDKKTGQLLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTLSEMMKESVLDAYGRALN
A
>Mature_480_residues
AENYDVIIIGSGPGGYVTAVRSAQLGFKTAIVEREHLGGICLNWGCIPTKALLRSAEIMHYSDHLKDYGLKLDGKVSVDT
SAVVDRSRKVSLRLNGGVAFLMKKNKVDVIWGEAKLSKPGEVVVSKTAKTPMEPQPPVPKGVKGEGTYTAKHIILATGAR
PRALPGIEPDGKLIWTYFEAMVPKEMPKSLLVMGSGAIGIEFASFYRTMGADVTVVELLPAVMPVEDAEVSKFAQKQFEK
QGMKIILEAKVTKVEKGANSVTAHVEMKDGKVEKITADRMISAVGVQGNIENLGLEALGVKTDRGCIVVDGYGKTNVPGI
YAIGDVAGPPMLAHKAEHEGVVCVEKIAGFPGVHAIDKLKIPGCTYCNPQVASVGLTEAKAKAEGKDIRVGRFQFAANGK
AIALGEDQGFIKTIFDKKTGQLLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTLSEMMKESVLDAYGRALNA

Specific function: Lipoamide dehydrogenase is a component of the alpha- ketoacid dehydrogenase complexes [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=484, Percent_Identity=38.8429752066116, Blast_Score=301, Evalue=6e-82,
Organism=Homo sapiens, GI50301238, Length=483, Percent_Identity=28.9855072463768, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI22035672, Length=486, Percent_Identity=29.2181069958848, Blast_Score=125, Evalue=8e-29,
Organism=Homo sapiens, GI291045266, Length=493, Percent_Identity=26.9776876267748, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI33519430, Length=466, Percent_Identity=25.9656652360515, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI33519428, Length=466, Percent_Identity=25.9656652360515, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI33519426, Length=466, Percent_Identity=25.9656652360515, Blast_Score=112, Evalue=1e-24,
Organism=Homo sapiens, GI148277065, Length=466, Percent_Identity=25.9656652360515, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI148277071, Length=450, Percent_Identity=26.2222222222222, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI291045268, Length=486, Percent_Identity=25.5144032921811, Blast_Score=95, Evalue=1e-19,
Organism=Escherichia coli, GI1786307, Length=461, Percent_Identity=37.9609544468547, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI87082354, Length=487, Percent_Identity=28.952772073922, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789915, Length=478, Percent_Identity=31.3807531380753, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI87081717, Length=473, Percent_Identity=27.6955602536998, Blast_Score=159, Evalue=5e-40,
Organism=Escherichia coli, GI1788892, Length=188, Percent_Identity=29.2553191489362, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI32565766, Length=493, Percent_Identity=38.5395537525355, Blast_Score=310, Evalue=1e-84,
Organism=Caenorhabditis elegans, GI17557007, Length=486, Percent_Identity=25.9259259259259, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=463, Percent_Identity=26.9978401727862, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71983419, Length=463, Percent_Identity=26.9978401727862, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI71982272, Length=493, Percent_Identity=25.1521298174442, Blast_Score=99, Evalue=6e-21,
Organism=Caenorhabditis elegans, GI17559934, Length=192, Percent_Identity=31.25, Blast_Score=67, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6321091, Length=486, Percent_Identity=36.4197530864198, Blast_Score=283, Evalue=3e-77,
Organism=Saccharomyces cerevisiae, GI6325166, Length=485, Percent_Identity=29.2783505154639, Blast_Score=176, Evalue=1e-44,
Organism=Saccharomyces cerevisiae, GI6325240, Length=493, Percent_Identity=27.1805273833671, Blast_Score=164, Evalue=3e-41,
Organism=Drosophila melanogaster, GI21358499, Length=482, Percent_Identity=40.4564315352697, Blast_Score=312, Evalue=3e-85,
Organism=Drosophila melanogaster, GI24640549, Length=483, Percent_Identity=25.6728778467909, Blast_Score=119, Evalue=6e-27,
Organism=Drosophila melanogaster, GI24640551, Length=498, Percent_Identity=26.3052208835341, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24640553, Length=483, Percent_Identity=25.6728778467909, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI17737741, Length=503, Percent_Identity=25.2485089463221, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 51499; Mature: 51367

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAENYDVIIIGSGPGGYVTAVRSAQLGFKTAIVEREHLGGICLNWGCIPTKALLRSAEIM
CCCCEEEEEEECCCCCEEEEEEHHHCCHHHHEEEHHHCCCEEEECCCCCHHHHHHHHHHH
HYSDHLKDYGLKLDGKVSVDTSAVVDRSRKVSLRLNGGVAFLMKKNKVDVIWGEAKLSKP
HHHHHHHHCCEEECCEEECCHHHHHCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCCC
GEVVVSKTAKTPMEPQPPVPKGVKGEGTYTAKHIILATGARPRALPGIEPDGKLIWTYFE
CCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEHHH
AMVPKEMPKSLLVMGSGAIGIEFASFYRTMGADVTVVELLPAVMPVEDAEVSKFAQKQFE
HHCCHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHH
KQGMKIILEAKVTKVEKGANSVTAHVEMKDGKVEKITADRMISAVGVQGNIENLGLEALG
HCCCEEEEEEEHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCCHHHCCEEEEE
VKTDRGCIVVDGYGKTNVPGIYAIGDVAGPPMLAHKAEHEGVVCVEKIAGFPGVHAIDKL
EECCCCEEEEECCCCCCCCEEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCHHHEE
KIPGCTYCNPQVASVGLTEAKAKAEGKDIRVGRFQFAANGKAIALGEDQGFIKTIFDKKT
CCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHCCCC
GQLLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTLSEMMKESVLDAYGRALN
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
A
C
>Mature Secondary Structure 
AENYDVIIIGSGPGGYVTAVRSAQLGFKTAIVEREHLGGICLNWGCIPTKALLRSAEIM
CCCEEEEEEECCCCCEEEEEEHHHCCHHHHEEEHHHCCCEEEECCCCCHHHHHHHHHHH
HYSDHLKDYGLKLDGKVSVDTSAVVDRSRKVSLRLNGGVAFLMKKNKVDVIWGEAKLSKP
HHHHHHHHCCEEECCEEECCHHHHHCCCEEEEEEECCCEEEEEECCCEEEEECCCCCCCC
GEVVVSKTAKTPMEPQPPVPKGVKGEGTYTAKHIILATGARPRALPGIEPDGKLIWTYFE
CCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEEEEEECCCCCCCCCCCCCCCEEEEHHH
AMVPKEMPKSLLVMGSGAIGIEFASFYRTMGADVTVVELLPAVMPVEDAEVSKFAQKQFE
HHCCHHCCCEEEEEECCCCHHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHH
KQGMKIILEAKVTKVEKGANSVTAHVEMKDGKVEKITADRMISAVGVQGNIENLGLEALG
HCCCEEEEEEEHHHHHCCCCCEEEEEEECCCCEEEEEHHHHHHHHCCCCCHHHCCEEEEE
VKTDRGCIVVDGYGKTNVPGIYAIGDVAGPPMLAHKAEHEGVVCVEKIAGFPGVHAIDKL
EECCCCEEEEECCCCCCCCEEEEECCCCCCCHHHCCCCCCCEEEEHHHCCCCCCCHHHEE
KIPGCTYCNPQVASVGLTEAKAKAEGKDIRVGRFQFAANGKAIALGEDQGFIKTIFDKKT
CCCCCCCCCCCHHCCCCHHHHHCCCCCCEEEEEEEEECCCCEEEEECCCCHHHHHHCCCC
GQLLGAHMVGAEVTELIQGFVVAMNLETTEEELMHTIFPHPTLSEMMKESVLDAYGRALN
HHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9515924 [H]