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The map label for this gene is eno

Identifier: 13470615

GI number: 13470615

Start: 267476

End: 268750

Strand: Direct

Name: eno

Synonym: mlr0378

Alternate gene names: 13470615

Gene position: 267476-268750 (Clockwise)

Preceding gene: 13470613

Following gene: 13470618

Centisome position: 3.8

GC content: 62.67

Gene sequence:

>1275_bases
ATGACCGCCATCATCGACATTGTCGGACGTGAAATCCTGGACAGCCGTGGAAACCCGACCGTCGAGGTCGATGTTGTACT
TGAAGACGGTTCGATGGGCCGCGCCGCGGTGCCGTCGGGCGCCTCGACCGGCGCCCATGAAGCCGTCGAGCTTCGCGACG
GCGGCGCCCGCTATCTCGGCAAGGGCGTGCTCAAGGCCGTAGAGGCGGTCAATGGCGAGCTGTTCGAAGCGATCGGCGGC
ATGGAAGCCGAAAACCAGATCCATATCGATCAGACCATGATCGAGCTTGATGGCACGCCCAACAAGAGCCGGCTCGGCGC
CAATGCCATTCTCGGCGTTTCGCTGGCGGTGGCCAAGGCCGCGGCCGATGCCGCCGGCCTGCCGCTCTATCGCTATGTCG
GCGGCACCAAGGCGCACATTCTGCCTGTGCCGATGATGAACATCATCAATGGCGGCGCCCATGCCGACAATCCGATCGAC
TTCCAGGAATTCATGATCCTGCCGGTCGGCGCGCCGACTTTGCGTGAAGGCGTGCGCTGGGGCTCGGAAATCTTCCACAC
GCTGCGCAAGAAGCTGAAGGATGCCGGCCACAACACCAATGTCGGCGACGAGGGCGGCTTTGCCCCGAACCTGAAGAGCG
CGCCGGTGGCGCTCGATTTCATCATGGAATCGATCGAGAAGGCCGGCTTCAAGCCGGGCGAGGAAATCGCGCTCGGCCTC
GATTGCGCCGCGACCGAGTTCTTCAAGGACGGCAATTACGTCTATGAAGGCGAGAAGAAGACGCGCGATCCCAAGGCCCA
GGCCAAGTACCTTGCCAAGCTCGCCGCCGACTATCCGATCGTCTCGATCGAGGACGGCCTTGCCGAGGACGACTGGGAAG
GCTGGAAATATCTGACCGACTTGATCGGCAAGAAGACACAGCTGGTCGGCGACGACCTTTTCGTCACCAACACGGCGCGG
CTGCGCGACGGCATCCGCATGGGCGTTGCCAATTCGATCCTGGTCAAGGTCAACCAGATCGGCTCGCTGACGGAAACGCT
CGATGCCGTCGAGACCGCGCACAAGGCTGGCTACACCGCCGTCATGTCGCACCGTTCGGGCGAGACCGAGGATTCGACCA
TCGCCGATCTCGCCGTCGCCACCAATTGCGGGCAGATCAAGACCGGCTCGCTGTCGCGCTCCGACCGCATGGCCAAATAC
AACCAGCTGATCCGCATCGAGGAAGAGCTTGGCAAGCAGGCGCGTTACGCCGGCAAGTCGGTGATCAAGGGCTGA

Upstream 100 bases:

>100_bases
ATCGGGCCGGCGGCACTATTCCGCCATGGCCGGATTGCCTTTTGCGTCGCTTTGGCTAAGACGGGCGCGACCTTTCGTCA
GATCAATCGGGGACATCGCA

Downstream 100 bases:

>100_bases
TCCGGAGCAAATTTCCAGGGAATGTGTAGGGCGGGTCATTCGCCCACCGCCAACGCGCGTCACACGGCCTCATGTGACCG
CGCTGCGCACCCTCGGTCCT

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTGAHEAVELRDGGARYLGKGVLKAVEAVNGELFEAIGG
MEAENQIHIDQTMIELDGTPNKSRLGANAILGVSLAVAKAAADAAGLPLYRYVGGTKAHILPVPMMNIINGGAHADNPID
FQEFMILPVGAPTLREGVRWGSEIFHTLRKKLKDAGHNTNVGDEGGFAPNLKSAPVALDFIMESIEKAGFKPGEEIALGL
DCAATEFFKDGNYVYEGEKKTRDPKAQAKYLAKLAADYPIVSIEDGLAEDDWEGWKYLTDLIGKKTQLVGDDLFVTNTAR
LRDGIRMGVANSILVKVNQIGSLTETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRMAKY
NQLIRIEEELGKQARYAGKSVIKG

Sequences:

>Translated_424_residues
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTGAHEAVELRDGGARYLGKGVLKAVEAVNGELFEAIGG
MEAENQIHIDQTMIELDGTPNKSRLGANAILGVSLAVAKAAADAAGLPLYRYVGGTKAHILPVPMMNIINGGAHADNPID
FQEFMILPVGAPTLREGVRWGSEIFHTLRKKLKDAGHNTNVGDEGGFAPNLKSAPVALDFIMESIEKAGFKPGEEIALGL
DCAATEFFKDGNYVYEGEKKTRDPKAQAKYLAKLAADYPIVSIEDGLAEDDWEGWKYLTDLIGKKTQLVGDDLFVTNTAR
LRDGIRMGVANSILVKVNQIGSLTETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRMAKY
NQLIRIEEELGKQARYAGKSVIKG
>Mature_423_residues
TAIIDIVGREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTGAHEAVELRDGGARYLGKGVLKAVEAVNGELFEAIGGM
EAENQIHIDQTMIELDGTPNKSRLGANAILGVSLAVAKAAADAAGLPLYRYVGGTKAHILPVPMMNIINGGAHADNPIDF
QEFMILPVGAPTLREGVRWGSEIFHTLRKKLKDAGHNTNVGDEGGFAPNLKSAPVALDFIMESIEKAGFKPGEEIALGLD
CAATEFFKDGNYVYEGEKKTRDPKAQAKYLAKLAADYPIVSIEDGLAEDDWEGWKYLTDLIGKKTQLVGDDLFVTNTARL
RDGIRMGVANSILVKVNQIGSLTETLDAVETAHKAGYTAVMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRMAKYN
QLIRIEEELGKQARYAGKSVIKG

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=435, Percent_Identity=51.4942528735632, Blast_Score=412, Evalue=1e-115,
Organism=Homo sapiens, GI4503571, Length=435, Percent_Identity=49.6551724137931, Blast_Score=398, Evalue=1e-111,
Organism=Homo sapiens, GI301897477, Length=434, Percent_Identity=50, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI301897469, Length=434, Percent_Identity=50, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI301897479, Length=432, Percent_Identity=45.1388888888889, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI169201331, Length=335, Percent_Identity=25.0746268656716, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI169201757, Length=335, Percent_Identity=25.0746268656716, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI239744207, Length=335, Percent_Identity=25.0746268656716, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1789141, Length=430, Percent_Identity=62.3255813953488, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI71995829, Length=437, Percent_Identity=51.0297482837529, Blast_Score=395, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI17536383, Length=437, Percent_Identity=51.0297482837529, Blast_Score=394, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI32563855, Length=193, Percent_Identity=47.1502590673575, Blast_Score=172, Evalue=2e-43,
Organism=Saccharomyces cerevisiae, GI6324974, Length=432, Percent_Identity=48.8425925925926, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324969, Length=432, Percent_Identity=48.8425925925926, Blast_Score=381, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6323985, Length=432, Percent_Identity=48.3796296296296, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6321693, Length=441, Percent_Identity=47.3922902494331, Blast_Score=375, Evalue=1e-105,
Organism=Saccharomyces cerevisiae, GI6321968, Length=441, Percent_Identity=46.7120181405896, Blast_Score=357, Evalue=1e-99,
Organism=Drosophila melanogaster, GI24580918, Length=436, Percent_Identity=51.8348623853211, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580916, Length=436, Percent_Identity=51.8348623853211, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580920, Length=436, Percent_Identity=51.8348623853211, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24580914, Length=436, Percent_Identity=51.8348623853211, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI281360527, Length=436, Percent_Identity=51.8348623853211, Blast_Score=378, Evalue=1e-105,
Organism=Drosophila melanogaster, GI17137654, Length=436, Percent_Identity=51.8348623853211, Blast_Score=378, Evalue=1e-105,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_RHILO (Q98MZ3)

Other databases:

- EMBL:   BA000012
- RefSeq:   NP_102184.1
- ProteinModelPortal:   Q98MZ3
- SMR:   Q98MZ3
- GeneID:   1224847
- GenomeReviews:   BA000012_GR
- KEGG:   mlo:mlr0378
- NMPDR:   fig|266835.1.peg.289
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BRENDA:   4.2.1.11
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 45271; Mature: 45140

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 204-204 ACT_SITE 336-336 BINDING 154-154 BINDING 163-163 BINDING 284-284 BINDING 311-311 BINDING 336-336 BINDING 387-387

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAIIDIVGREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTGAHEAVELRDGGARYLG
CCHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVLKAVEAVNGELFEAIGGMEAENQIHIDQTMIELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AADAAGLPLYRYVGGTKAHILPVPMMNIINGGAHADNPIDFQEFMILPVGAPTLREGVRW
HHHHCCCCHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHHH
GSEIFHTLRKKLKDAGHNTNVGDEGGFAPNLKSAPVALDFIMESIEKAGFKPGEEIALGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEEC
DCAATEFFKDGNYVYEGEKKTRDPKAQAKYLAKLAADYPIVSIEDGLAEDDWEGWKYLTD
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
LIGKKTQLVGDDLFVTNTARLRDGIRMGVANSILVKVNQIGSLTETLDAVETAHKAGYTA
HHCCHHHHCCCCEEEECHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRMAKYNQLIRIEEELGKQARYAGKS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
VIKG
HCCC
>Mature Secondary Structure 
TAIIDIVGREILDSRGNPTVEVDVVLEDGSMGRAAVPSGASTGAHEAVELRDGGARYLG
CHHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCHHHHH
KGVLKAVEAVNGELFEAIGGMEAENQIHIDQTMIELDGTPNKSRLGANAILGVSLAVAKA
HHHHHHHHHHCHHHHHHHCCCCCCCEEEEEEEEEEECCCCCHHHCCCHHHHHHHHHHHHH
AADAAGLPLYRYVGGTKAHILPVPMMNIINGGAHADNPIDFQEFMILPVGAPTLREGVRW
HHHHCCCCHHHHCCCCCEEEECCCHHHHHCCCCCCCCCCCHHHEEEEECCCHHHHHHHHH
GSEIFHTLRKKLKDAGHNTNVGDEGGFAPNLKSAPVALDFIMESIEKAGFKPGEEIALGL
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHEEEEC
DCAATEFFKDGNYVYEGEKKTRDPKAQAKYLAKLAADYPIVSIEDGLAEDDWEGWKYLTD
HHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCHHHHHHHH
LIGKKTQLVGDDLFVTNTARLRDGIRMGVANSILVKVNQIGSLTETLDAVETAHKAGYTA
HHCCHHHHCCCCEEEECHHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCHH
VMSHRSGETEDSTIADLAVATNCGQIKTGSLSRSDRMAKYNQLIRIEEELGKQARYAGKS
EEECCCCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
VIKG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11214968