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The map label for this gene is cpdB [H]

Identifier: 13470608

GI number: 13470608

Start: 259897

End: 261876

Strand: Direct

Name: cpdB [H]

Synonym: mlr0366

Alternate gene names: 13470608

Gene position: 259897-261876 (Clockwise)

Preceding gene: 13470607

Following gene: 13470610

Centisome position: 3.69

GC content: 61.36

Gene sequence:

>1980_bases
ATGTCGCAACTGCTCCATCCCGTCTCCCGCCGCGGTTTCCTCGCTGGCGCCGCAGCCACAGGGGCGCTGATCGTGCTTCA
TCCCTTCTCCGCCCGGGCGCAGGCCAACCAGGCGCATCTTCGGATCATGGAAACCACCGACATCCATGTGAACGTTCTGC
CTTACGATTACTACGCCGACAAAGCCAACGACACGATGGGTCTGTCGCGCACTGCATCGCTGATCGACGCGGTGCGCAAG
GAAGCCGTCAATTCGATGCTGATCGACAATGGCGACCTGCTGCAAGGCAACCCGATGGGCGACTACATCGCCTACGAGAA
AGGCCTGAAGGACGGCGATCTGCATCCGGTCATGAAAGGCATGAACCTGCTGGGCTACGAGTGCTCGACACTCGGCAACC
ACGAGTTCAACTATGGCTTGTCTTTCCTGGACAAGGTGCTGGCCGGCGCCAATTTCCCCTTCGTCTGCGCCAATCTGATC
CGCGGCACCGAGCTTGCCGCCAACCCGCGCGACGACAAGCTCTATCTCAAGCCCTACGTGATCCTCGACAGGAAGATCAA
GGACGGTTCGGGTGCCGAGCAGCCGATCCGGATCGGCATTATCGGCTTCGTACCGCCGCAGATCATGGTCTGGGACCTCA
AGAATCTCGAAGGCAACGTCAAGACGCGCGACATCGTCGAGGCGGCCACGGCCTGGGTGCCACAGATGAAGGAAGAGGGC
GCCGACATCGTCATCGCGCTCTCGCACTCCGGCATCGATGTGAAGCAGGGCGACATGATGGAGAACGCCTCGTTCTTTGT
TGCCGGCGTCGATGGCATCGATGCGGTTTTCACCGGCCACCAGCATCTGGTGTTCCCCGGCAAGAAGGACTTTCAGTCGC
TCGAAGGCGTCGACACGCAGAAAGGCACGCTGCAGGGCAAGCCCGCCGTGATGGGCGGTTTCTGGGGCTCGCATATGGGG
CTGATCGACCTGATGCTCGAGCGTGACGGATCGAAATGGAAGGTCGTCTCCGCGACTTCCGAGGCGCGGCCGATCTTCGA
GCGCGTCGACAACAAGGCCAAGCCGACGGTTTCCGACGACAAGCGCATCATCGCCGCCCTCGAGCAGGACCACCAGGCGA
CCCTGGCCTATGTGCGCCGTCCGGTCGGCAAGACCTCCGCGCCGCTCTATTCCTATTTCGCGCTGGTCGCCGACGATCCG
TCGGTGCAGGTCGTCAGCCAGGCGCAGACCTGGTATCTGAAGGATATTCTCAAGAGCACGCAGTGGAAGGACGTGCCGCT
GCTGTCGGCCGCCGCTCCCTTCAAGGCGGGCGGGCGCAACGGCGCCGACTACTACACCGACGTGCCGGCGGGCGACATCG
CCATCAAGAACGTCGCCGACCTCTATCTCTATCCCAACACCGTGCGCGCCGTCGAAATCACCGGAGCGCAGGTCAAGGAA
TGGCTGGAAATGTCGGCCGGCATCTTCAACAGGATCGAGGCAGGGAAGGCTGACCAGGCACTCATCAACACCGATTTCCC
GTCCTACAATTTCGACGTCATCGACGGCGTCTCCTACAAGATAGATCTGTCGCAGCCGCCGAAATACGATGCCAAGGGTG
GGGTGGCGAATGCCGGCGCCAACCGCATCGTCGACTTGATGTTCGGCGGCAAACCGATTGATCCCAATCAGAAATTCGTC
GTCGCGACCAACAATTACCGGGCCGGCGGCGGCGGCAATTTCCCCGACATCAATGCCTCGAAGATCATCTATGAGGCGCC
GGACACCAACCGCGACGTCATCGTTCGCTACATTGTGAGCCAGGGCACGATCAACCCGTCGGCCGACGGCAACTGGTCGT
TCGGACCGCTGCCGGGAACCAGCGTCGTATTCGAGACAGGCGTCAAGGCAAAGGACTTCATCGCCGATGTGAAGTCGCTG
ATGATCGAGCCGGCGGGCGAGGGCGAAGCGGGGTTCGCAAAGTATCGCATCCTTCTCTGA

Upstream 100 bases:

>100_bases
GATGTGCGGATGCGCGCGACTTCTTGAGTGGACTTGAGCTGATTTCGGCGCGAAGTTCGCCTCGGGGCAGGGCGTGTCAG
CTTGTCAATGAGGAGAAAAC

Downstream 100 bases:

>100_bases
TCCGCATCGCACGGCATCAGTTCGCCGGCGTCAGCGGCAAGGCCGTCGCGGGAGCTGGCGGGCTGACCGGCAGTGTCGGC
TCGAGGGACGTACCGGTCGG

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 659; Mature: 658

Protein sequence:

>659_residues
MSQLLHPVSRRGFLAGAAATGALIVLHPFSARAQANQAHLRIMETTDIHVNVLPYDYYADKANDTMGLSRTASLIDAVRK
EAVNSMLIDNGDLLQGNPMGDYIAYEKGLKDGDLHPVMKGMNLLGYECSTLGNHEFNYGLSFLDKVLAGANFPFVCANLI
RGTELAANPRDDKLYLKPYVILDRKIKDGSGAEQPIRIGIIGFVPPQIMVWDLKNLEGNVKTRDIVEAATAWVPQMKEEG
ADIVIALSHSGIDVKQGDMMENASFFVAGVDGIDAVFTGHQHLVFPGKKDFQSLEGVDTQKGTLQGKPAVMGGFWGSHMG
LIDLMLERDGSKWKVVSATSEARPIFERVDNKAKPTVSDDKRIIAALEQDHQATLAYVRRPVGKTSAPLYSYFALVADDP
SVQVVSQAQTWYLKDILKSTQWKDVPLLSAAAPFKAGGRNGADYYTDVPAGDIAIKNVADLYLYPNTVRAVEITGAQVKE
WLEMSAGIFNRIEAGKADQALINTDFPSYNFDVIDGVSYKIDLSQPPKYDAKGGVANAGANRIVDLMFGGKPIDPNQKFV
VATNNYRAGGGGNFPDINASKIIYEAPDTNRDVIVRYIVSQGTINPSADGNWSFGPLPGTSVVFETGVKAKDFIADVKSL
MIEPAGEGEAGFAKYRILL

Sequences:

>Translated_659_residues
MSQLLHPVSRRGFLAGAAATGALIVLHPFSARAQANQAHLRIMETTDIHVNVLPYDYYADKANDTMGLSRTASLIDAVRK
EAVNSMLIDNGDLLQGNPMGDYIAYEKGLKDGDLHPVMKGMNLLGYECSTLGNHEFNYGLSFLDKVLAGANFPFVCANLI
RGTELAANPRDDKLYLKPYVILDRKIKDGSGAEQPIRIGIIGFVPPQIMVWDLKNLEGNVKTRDIVEAATAWVPQMKEEG
ADIVIALSHSGIDVKQGDMMENASFFVAGVDGIDAVFTGHQHLVFPGKKDFQSLEGVDTQKGTLQGKPAVMGGFWGSHMG
LIDLMLERDGSKWKVVSATSEARPIFERVDNKAKPTVSDDKRIIAALEQDHQATLAYVRRPVGKTSAPLYSYFALVADDP
SVQVVSQAQTWYLKDILKSTQWKDVPLLSAAAPFKAGGRNGADYYTDVPAGDIAIKNVADLYLYPNTVRAVEITGAQVKE
WLEMSAGIFNRIEAGKADQALINTDFPSYNFDVIDGVSYKIDLSQPPKYDAKGGVANAGANRIVDLMFGGKPIDPNQKFV
VATNNYRAGGGGNFPDINASKIIYEAPDTNRDVIVRYIVSQGTINPSADGNWSFGPLPGTSVVFETGVKAKDFIADVKSL
MIEPAGEGEAGFAKYRILL
>Mature_658_residues
SQLLHPVSRRGFLAGAAATGALIVLHPFSARAQANQAHLRIMETTDIHVNVLPYDYYADKANDTMGLSRTASLIDAVRKE
AVNSMLIDNGDLLQGNPMGDYIAYEKGLKDGDLHPVMKGMNLLGYECSTLGNHEFNYGLSFLDKVLAGANFPFVCANLIR
GTELAANPRDDKLYLKPYVILDRKIKDGSGAEQPIRIGIIGFVPPQIMVWDLKNLEGNVKTRDIVEAATAWVPQMKEEGA
DIVIALSHSGIDVKQGDMMENASFFVAGVDGIDAVFTGHQHLVFPGKKDFQSLEGVDTQKGTLQGKPAVMGGFWGSHMGL
IDLMLERDGSKWKVVSATSEARPIFERVDNKAKPTVSDDKRIIAALEQDHQATLAYVRRPVGKTSAPLYSYFALVADDPS
VQVVSQAQTWYLKDILKSTQWKDVPLLSAAAPFKAGGRNGADYYTDVPAGDIAIKNVADLYLYPNTVRAVEITGAQVKEW
LEMSAGIFNRIEAGKADQALINTDFPSYNFDVIDGVSYKIDLSQPPKYDAKGGVANAGANRIVDLMFGGKPIDPNQKFVV
ATNNYRAGGGGNFPDINASKIIYEAPDTNRDVIVRYIVSQGTINPSADGNWSFGPLPGTSVVFETGVKAKDFIADVKSLM
IEPAGEGEAGFAKYRILL

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=645, Percent_Identity=47.2868217054264, Blast_Score=579, Evalue=1e-166,
Organism=Escherichia coli, GI1786687, Length=587, Percent_Identity=22.3168654173765, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 71578; Mature: 71447

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLLHPVSRRGFLAGAAATGALIVLHPFSARAQANQAHLRIMETTDIHVNVLPYDYYAD
CCHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCCEEEEEEEECCEEEEEEEEHHCCC
KANDTMGLSRTASLIDAVRKEAVNSMLIDNGDLLQGNPMGDYIAYEKGLKDGDLHPVMKG
CCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEECCCCCCCHHHHHCCCCCCCCHHHHHC
MNLLGYECSTLGNHEFNYGLSFLDKVLAGANFPFVCANLIRGTELAANPRDDKLYLKPYV
CHHCCEEHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEE
ILDRKIKDGSGAEQPIRIGIIGFVPPQIMVWDLKNLEGNVKTRDIVEAATAWVPQMKEEG
EEEEEECCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
ADIVIALSHSGIDVKQGDMMENASFFVAGVDGIDAVFTGHQHLVFPGKKDFQSLEGVDTQ
CCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCEEECCEEEECCCCHHHHHHCCCCCC
KGTLQGKPAVMGGFWGSHMGLIDLMLERDGSKWKVVSATSEARPIFERVDNKAKPTVSDD
CCCCCCCCCEEECCCCCHHHHEEHEEECCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCC
KRIIAALEQDHQATLAYVRRPVGKTSAPLYSYFALVADDPSVQVVSQAQTWYLKDILKST
HHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCEEEEHHHHHHHHHHHHHCC
QWKDVPLLSAAAPFKAGGRNGADYYTDVPAGDIAIKNVADLYLYPNTVRAVEITGAQVKE
CCCCCCEEECCCCCCCCCCCCCCEEECCCCCCEECCCCEEEEEECCCEEEEEECHHHHHH
WLEMSAGIFNRIEAGKADQALINTDFPSYNFDVIDGVSYKIDLSQPPKYDAKGGVANAGA
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCC
NRIVDLMFGGKPIDPNQKFVVATNNYRAGGGGNFPDINASKIIYEAPDTNRDVIVRYIVS
CCEEEEEECCCCCCCCCCEEEEECCEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEC
QGTINPSADGNWSFGPLPGTSVVFETGVKAKDFIADVKSLMIEPAGEGEAGFAKYRILL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure 
SQLLHPVSRRGFLAGAAATGALIVLHPFSARAQANQAHLRIMETTDIHVNVLPYDYYAD
CHHHHHHHHCCEEEECCCCCEEEEEECCCCCCCCCCEEEEEEEECCEEEEEEEEHHCCC
KANDTMGLSRTASLIDAVRKEAVNSMLIDNGDLLQGNPMGDYIAYEKGLKDGDLHPVMKG
CCCCCCCHHHHHHHHHHHHHHHHHHEEECCCCEECCCCCCCHHHHHCCCCCCCCHHHHHC
MNLLGYECSTLGNHEFNYGLSFLDKVLAGANFPFVCANLIRGTELAANPRDDKLYLKPYV
CHHCCEEHHCCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCCCEEEEEEEE
ILDRKIKDGSGAEQPIRIGIIGFVPPQIMVWDLKNLEGNVKTRDIVEAATAWVPQMKEEG
EEEEEECCCCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCC
ADIVIALSHSGIDVKQGDMMENASFFVAGVDGIDAVFTGHQHLVFPGKKDFQSLEGVDTQ
CCEEEEEECCCCCCCCCCCCCCCCEEEEECCCCCCEEECCEEEECCCCHHHHHHCCCCCC
KGTLQGKPAVMGGFWGSHMGLIDLMLERDGSKWKVVSATSEARPIFERVDNKAKPTVSDD
CCCCCCCCCEEECCCCCHHHHEEHEEECCCCEEEEEECCCCCCHHHHHCCCCCCCCCCCC
KRIIAALEQDHQATLAYVRRPVGKTSAPLYSYFALVADDPSVQVVSQAQTWYLKDILKST
HHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHEEEEEECCCCEEEEHHHHHHHHHHHHHCC
QWKDVPLLSAAAPFKAGGRNGADYYTDVPAGDIAIKNVADLYLYPNTVRAVEITGAQVKE
CCCCCCEEECCCCCCCCCCCCCCEEECCCCCCEECCCCEEEEEECCCEEEEEECHHHHHH
WLEMSAGIFNRIEAGKADQALINTDFPSYNFDVIDGVSYKIDLSQPPKYDAKGGVANAGA
HHHHHHHHHHHHCCCCCCCEEEECCCCCCCCEEECCEEEEEECCCCCCCCCCCCCCCCCC
NRIVDLMFGGKPIDPNQKFVVATNNYRAGGGGNFPDINASKIIYEAPDTNRDVIVRYIVS
CCEEEEEECCCCCCCCCCEEEEECCEECCCCCCCCCCCCCEEEEECCCCCCCEEEEEEEC
QGTINPSADGNWSFGPLPGTSVVFETGVKAKDFIADVKSLMIEPAGEGEAGFAKYRILL
CCCCCCCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]