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The map label for this gene is sseA [H]

Identifier: 13470599

GI number: 13470599

Start: 251420

End: 252301

Strand: Direct

Name: sseA [H]

Synonym: mlr0355

Alternate gene names: 13470599

Gene position: 251420-252301 (Clockwise)

Preceding gene: 13470598

Following gene: 13470600

Centisome position: 3.57

GC content: 65.87

Gene sequence:

>882_bases
ATGGCCCAGGACAGTCCTTTCACCGTCGATGCGGACTGGCTGCAGGCGCGCCTGGGCGAGCCCGGCCTGACCATCGTGGA
CGCATCCTGGTACCTGCCGGCGCAAAAGCGCGACGCGCGCGCCGAATATAATGCAGCGCACATCCCGGGCGCCCGTTTCC
TGGATCAGGACGCGGTGTCGGATCCCGACGCTGCTTTGCCGCACACGCTGCCTTCGCCTCAGCATTTCGCGCAATATGTT
GGTTCGATGGGCGTGTCCGCCGATGACACCATTGTCGTCTATGATGGTCCCGGCTTTTTCTCGGCACCCCGGGCATGGTG
GATGTTCCGCATTATGGGTGTTTTCCAGACCTATATCCTGGATGGCGGATTCGACGCCTGGAAAACGGCCGGCCGGCCGG
TAACGGCCGAGCCGACGAAGATCGCGCCAAGCGTGTTCCATGCCGATTTCGACGCCGGCCGCGTCGCCAGCCTCGCCGAC
ATGCGCCGGATCGTCGAGACCAGCGCCAGCCAGATTGCCGACGCCCGTGGACCCGGCCGCTTCACCGGCGCCGAGCCCGA
GCCCCGCGCGGGTATCCGCTCCGGCCATATGCCGGGAGCGCGCAACGTACCGTACTCCGCCCTGTCGGAAAACGGCATGC
TGTTGTCGAAGGACCGTTTGCGCAAGGTGATCGAGGAGGCCGGCATTGACCTGTCGAAGCCCGTCGTCACATCTTGCGGC
TCCGGTGTCACCGCCGCGGTGGTTACGCTGGCGCTGGAGACGCTTGGCCACACCGACAACAGGCTCTATGACGGCTCATG
GACGGAATGGGGCGGGCTCTCCGACACGCCTGTCGTGACCGGCCCCGCGACGCCCGGTCCCGCTAAGACCGGCAAGGAAT
GA

Upstream 100 bases:

>100_bases
GAAGAAGGGCCGCGAGAACAGGCGCTTCCGCATCCGCTTCGGCCCGATGCCGGCAGCCTGAACACGCAACACTTGCTTAC
CCGGGATAGAGAGAACCAGA

Downstream 100 bases:

>100_bases
ACGCGATGGAAAACGGTGTGCTGCAGGACATTGAAGTCACGGTGACTTTTCTCGAAATGGATGCCCCTCCCGCCGTCTCC
CCGCCAGTGCCCTACAACCG

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 293; Mature: 292

Protein sequence:

>293_residues
MAQDSPFTVDADWLQARLGEPGLTIVDASWYLPAQKRDARAEYNAAHIPGARFLDQDAVSDPDAALPHTLPSPQHFAQYV
GSMGVSADDTIVVYDGPGFFSAPRAWWMFRIMGVFQTYILDGGFDAWKTAGRPVTAEPTKIAPSVFHADFDAGRVASLAD
MRRIVETSASQIADARGPGRFTGAEPEPRAGIRSGHMPGARNVPYSALSENGMLLSKDRLRKVIEEAGIDLSKPVVTSCG
SGVTAAVVTLALETLGHTDNRLYDGSWTEWGGLSDTPVVTGPATPGPAKTGKE

Sequences:

>Translated_293_residues
MAQDSPFTVDADWLQARLGEPGLTIVDASWYLPAQKRDARAEYNAAHIPGARFLDQDAVSDPDAALPHTLPSPQHFAQYV
GSMGVSADDTIVVYDGPGFFSAPRAWWMFRIMGVFQTYILDGGFDAWKTAGRPVTAEPTKIAPSVFHADFDAGRVASLAD
MRRIVETSASQIADARGPGRFTGAEPEPRAGIRSGHMPGARNVPYSALSENGMLLSKDRLRKVIEEAGIDLSKPVVTSCG
SGVTAAVVTLALETLGHTDNRLYDGSWTEWGGLSDTPVVTGPATPGPAKTGKE
>Mature_292_residues
AQDSPFTVDADWLQARLGEPGLTIVDASWYLPAQKRDARAEYNAAHIPGARFLDQDAVSDPDAALPHTLPSPQHFAQYVG
SMGVSADDTIVVYDGPGFFSAPRAWWMFRIMGVFQTYILDGGFDAWKTAGRPVTAEPTKIAPSVFHADFDAGRVASLADM
RRIVETSASQIADARGPGRFTGAEPEPRAGIRSGHMPGARNVPYSALSENGMLLSKDRLRKVIEEAGIDLSKPVVTSCGS
GVTAAVVTLALETLGHTDNRLYDGSWTEWGGLSDTPVVTGPATPGPAKTGKE

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=270, Percent_Identity=43.3333333333333, Blast_Score=232, Evalue=3e-61,
Organism=Homo sapiens, GI61835204, Length=270, Percent_Identity=43.3333333333333, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI194473681, Length=270, Percent_Identity=43.3333333333333, Blast_Score=231, Evalue=4e-61,
Organism=Homo sapiens, GI17402865, Length=270, Percent_Identity=42.5925925925926, Blast_Score=214, Evalue=8e-56,
Organism=Escherichia coli, GI87082121, Length=271, Percent_Identity=42.4354243542435, Blast_Score=220, Evalue=8e-59,
Organism=Escherichia coli, GI87081967, Length=286, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71997283, Length=245, Percent_Identity=29.7959183673469, Blast_Score=107, Evalue=8e-24,
Organism=Caenorhabditis elegans, GI17561888, Length=289, Percent_Identity=28.0276816608997, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17559150, Length=273, Percent_Identity=26.3736263736264, Blast_Score=91, Evalue=7e-19,
Organism=Caenorhabditis elegans, GI115534702, Length=266, Percent_Identity=26.3157894736842, Blast_Score=89, Evalue=4e-18,
Organism=Caenorhabditis elegans, GI17543836, Length=294, Percent_Identity=27.2108843537415, Blast_Score=81, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI17543838, Length=294, Percent_Identity=27.2108843537415, Blast_Score=81, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6324825, Length=268, Percent_Identity=35.4477611940299, Blast_Score=160, Evalue=3e-40,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 31271; Mature: 31140

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: PS00380 RHODANESE_1 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQDSPFTVDADWLQARLGEPGLTIVDASWYLPAQKRDARAEYNAAHIPGARFLDQDAVS
CCCCCCCEEEHHHHHHHCCCCCCEEEECCEECCCHHCCCHHHCCCCCCCCHHHHCCCCCC
DPDAALPHTLPSPQHFAQYVGSMGVSADDTIVVYDGPGFFSAPRAWWMFRIMGVFQTYIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
DGGFDAWKTAGRPVTAEPTKIAPSVFHADFDAGRVASLADMRRIVETSASQIADARGPGR
CCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FTGAEPEPRAGIRSGHMPGARNVPYSALSENGMLLSKDRLRKVIEEAGIDLSKPVVTSCG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECHHHHHHHHHHHCCCCCCCHHHHCC
SGVTAAVVTLALETLGHTDNRLYDGSWTEWGGLSDTPVVTGPATPGPAKTGKE
CCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure 
AQDSPFTVDADWLQARLGEPGLTIVDASWYLPAQKRDARAEYNAAHIPGARFLDQDAVS
CCCCCCEEEHHHHHHHCCCCCCEEEECCEECCCHHCCCHHHCCCCCCCCHHHHCCCCCC
DPDAALPHTLPSPQHFAQYVGSMGVSADDTIVVYDGPGFFSAPRAWWMFRIMGVFQTYIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
DGGFDAWKTAGRPVTAEPTKIAPSVFHADFDAGRVASLADMRRIVETSASQIADARGPGR
CCCCHHHHHCCCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
FTGAEPEPRAGIRSGHMPGARNVPYSALSENGMLLSKDRLRKVIEEAGIDLSKPVVTSCG
CCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCEEECHHHHHHHHHHHCCCCCCCHHHHCC
SGVTAAVVTLALETLGHTDNRLYDGSWTEWGGLSDTPVVTGPATPGPAKTGKE
CCHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]