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The map label for this gene is trmD

Identifier: 13358132

GI number: 13358132

Start: 707907

End: 708593

Strand: Reverse

Name: trmD

Synonym: UU567

Alternate gene names: 13358132

Gene position: 708593-707907 (Counterclockwise)

Preceding gene: 13358133

Following gene: 13358131

Centisome position: 94.26

GC content: 29.69

Gene sequence:

>687_bases
ATGAAAATATCGATTTTATCTTTATTTCCTGAATTATATGAAACATGGATAAAGCATTCAATTATTAGTAATGCTATTAA
AAATAACCAAGTTATAATAGAAGTTATCGATTTTCGTTTATATACAAATGATAAACATAAAAAAGTTGATGATTACCAAT
ATGGTGGCGGAGCAGGTATGGTTTTAAGGATTGAACCAATTGTTGCTGCCATTCGTGCAATTCGTTCTTCTAATTCATAT
GTGATTTTAACAACACCAAAAGGTCAAGTTTTTAATCAAGAATTAGCAAACAATTTTGTTAGTAAATATGATCATATCAT
CATTATTGCTGGTCATTATGAAGGTTTTGATGAACGTATTAATTATTACATTGACGCCCAATATAGTATCGGTGATTTTG
TCTTAACAGGGGGGGAATTACCAAGTATGGTAATTTCTGATGCTGTTATTCGTTTGTTAGATGGCGTTATTTCTCCTTCT
AGTTTAGAAACTGAATCATTTAATAACTACTTATTAGATTATCCTGTTTACACACGCCCTCTTGTTTTTGAAGGGCATAA
AGTACCGGATATACTATTATCTGGTCATCATAAAAATATTGCTGATTTTCGTAAACAGCAACAAGAGACGATTACTAAAA
AGAATCGTCCTGATTTATATCAAAAATATTTAAATAACAAAAAGTAG

Upstream 100 bases:

>100_bases
CTTATCACAAGAAGGTATTTGAAAAGAATTTATTGCTTTAAAAGATGCAAATAAAAAACGTAAAGCTGCTTTAAGTAAAG
CTAAATAATTTATAACATTA

Downstream 100 bases:

>100_bases
GTGTAAAAAATGGCTTTATTTAAGATTAACAAAGGCGAAATTATGAATTTTGTTAATTCAACACAATTAAAAACAGATAT
TCCTAATTTTGATTCTGGTG

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGMVLRIEPIVAAIRAIRSSNSY
VILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERINYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPS
SLETESFNNYLLDYPVYTRPLVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK

Sequences:

>Translated_228_residues
MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGMVLRIEPIVAAIRAIRSSNSY
VILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERINYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPS
SLETESFNNYLLDYPVYTRPLVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK
>Mature_228_residues
MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGMVLRIEPIVAAIRAIRSSNSY
VILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERINYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPS
SLETESFNNYLLDYPVYTRPLVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=230, Percent_Identity=40.8695652173913, Blast_Score=165, Evalue=2e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_UREP2 (B1AJK8)

Other databases:

- EMBL:   CP000942
- RefSeq:   YP_001752652.1
- ProteinModelPortal:   B1AJK8
- SMR:   B1AJK8
- GeneID:   6155387
- GenomeReviews:   CP000942_GR
- KEGG:   upa:UPA3_0606
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   UPAR505682:UPA3_0606-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 26134; Mature: 26134

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGM
CEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCEEECCCCEE
VLRIEPIVAAIRAIRSSNSYVILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERI
EEEEHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHCCE
NYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPSSLETESFNNYLLDYPVYTRP
EEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
LVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK
EEECCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MKISILSLFPELYETWIKHSIISNAIKNNQVIIEVIDFRLYTNDKHKKVDDYQYGGGAGM
CEEHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEEECCCCCCCCCEEECCCCEE
VLRIEPIVAAIRAIRSSNSYVILTTPKGQVFNQELANNFVSKYDHIIIIAGHYEGFDERI
EEEEHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCEEEEEEECCCCHHCCE
NYYIDAQYSIGDFVLTGGELPSMVISDAVIRLLDGVISPSSLETESFNNYLLDYPVYTRP
EEEEEEEECCCCEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCC
LVFEGHKVPDILLSGHHKNIADFRKQQQETITKKNRPDLYQKYLNNKK
EEECCCCCCHHEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA