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The map label for this gene is fusA

Identifier: 13358087

GI number: 13358087

Start: 648562

End: 650628

Strand: Reverse

Name: fusA

Synonym: UU523

Alternate gene names: 13358087

Gene position: 650628-648562 (Counterclockwise)

Preceding gene: 13358088

Following gene: 13358086

Centisome position: 86.55

GC content: 33.77

Gene sequence:

>2067_bases
ATGTCTAAAGAATTAAAATTATTTCGTAACTTTGGAATCATGGCACACATTGATGCTGGTAAAACAACAACCTCTGAACG
GATTTTATATCATACAGGAAAGAATCATAAAATCGGAGAGACTCACGATGGTGCTGCAACAATGGATTGAATGGCACAAG
AAAAAGAACGTGGAATTACCATTACATCAGCAGCAACTTATGCAAAATGAAAAGGTCATAGTTTAAATTTAATTGATACA
CCGGGACACGTTGATTTTACTGTTGAAGTAGAACGTTCTTTGCGTGTTTTAGATGGAGCTGTCGCTGTTCTAGATGGTCA
AAATGGAGTTGAACCACAAACTGAAACAGTCTGAAGACAAGCTACTAAATATAATGTTCCAAGAATCGTTTTTGTTAATA
AGATGGATAAAACAGGTGCTGATTTTTATTACTCAATTGAGACAATGAAGAATCGTTTAGGTGTTAAAGCCACAGCTATT
CAAATTCCAATTGGTGCTGAAGCCGATTTTGTTGGAAGTATTGATTTAATTGAAATGAAAGCTTATATTTATGATGGAAA
AGCTGATGAAGAATATAAAATTGAAGATATTCCAGCTGATTACTTAACAAAAGCACAAGTAATGCGTTCACAAATGATCG
ATGATGTAGCTGTTTTTGATGATGAAGTGATGGAAAAATACTTATCTGGTGAAGAACTAAGTCATGAAGATATTAAAAAA
TGTATTCGTAAAGGAGTAATTTCAACTGAATTATACCCAGTATTATGTGGTACAGCTTTTAAAAACAAAGGTGTTAAAAA
ATTATTAGACGCAGTTGTTGATTTCTTACCTTCACCAATTGATGTTCCACCAATTAAAGGTGTTGATGATCATGGTAATC
CAATTGAATATCATAATGATCCAAATGAACCATTTGCTGCCCTTGCCTTCAAAGTCGCAACTGATCCTTTTGTTGGACGT
TTAACATATATTCGTGTATATAGTGGTAAACTTGACAAAGGTACTTATATTTACAATGCAACAAAAGATAAAAAAGAAAG
AATTTCTCGTCTTGTAAAAATGCATTCAAACAACCGTGATGAGATTGATTCAATTAGTGCTGGTGATATTTGTGCTGTAA
TTGGATTAAAAGACACTACTACTGGTGATACAATTTGTGATGAAAAAAAACCAGTTATTTTAGAACAAATGGTATTTGCA
GAACCTGTTATTTCTTTATCTGTTGAACCAAAAACTAAAGCAGATCAAGAAAAGATGAGTTTAGCTTTATCTAAATTAGC
TGAAGAAGATCCAACTTTTAGAACATACACAAATGAAGAAACAGGTCAAACTATTATTGCTGGAATGGGTGAATTACATT
TAGATGTTTTGGTTGATCGTATGCGTCGTGAATTTAATGTTCAAGTTAATGTAGGTGCTCCTCAAGTTAGTTATCGTGAG
ACATTTACTGAAGTTGCTGATGCTGAAGGTAAGTATATTAAGCAATCTGGTGGACGTGGTCAATATGGGCATGTCTGAAT
TAAATTTGAACCTAATCATGATAAAGGATTTGAATTTGTTGACAACATTGTAGGTGGTAAAGTACCAAAAGAATATATTA
AAGAAGTTGAAAACGGCTTGATTGAGGCATTAACATCAGGGCCAATTGCTGGTTATCAAACAATTGACGTTAAAGCAACT
ATTTTTGATGGTTCATATCATGATGTTGATTCATCTGGTATGGCTTATAAAATTGCTGCATCTCTTGCTTTTAAAGAAGC
AGCAAAAGTATGCAAACCAGTTTTATTAGAACCTATTATGTCAGTTGATGTAACTACACCTGATGATTATTTTGGGACGG
TAATGGGCGATATTTCAAAACGTCGTGGTGTAATTGAAGGACAAGAACAACGTGGTAATGCACAAGCTATTAAAGCTAAA
GTTCCGCTATCAGAAATGTTTGGATATGCAACGGATTTACGTTCAAATACACAAGGACGTGGTCAATATATTATGCAATT
CAGTCACTATGCACAAGCACCTAAGTCTGTTACTGAAGAAGTAATGGTAGCACGTGCAAAAAAATAA

Upstream 100 bases:

>100_bases
CAGCAATTAAGAAAAAAGAAGATACTCATAAAATGGCAGAAGCAAATAAAGCTTTTGCACACCTACGTTGATAAAATTTG
TTTTAAGGAATTGTTAATAT

Downstream 100 bases:

>100_bases
TTATTTTAACAATTAACAATAAAGTATTTAATTAATAATCTAAAAAGTTAAAATGTTATAATTATAAGGTATAATACCTT
TTATTATGTATTCAAAAATT

Product: elongation factor G

Products: GDP; phosphate

Alternate protein names: EF-G [H]

Number of amino acids: Translated: 688; Mature: 687

Protein sequence:

>688_residues
MSKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMDWMAQEKERGITITSAATYAKWKGHSLNLIDT
PGHVDFTVEVERSLRVLDGAVAVLDGQNGVEPQTETVWRQATKYNVPRIVFVNKMDKTGADFYYSIETMKNRLGVKATAI
QIPIGAEADFVGSIDLIEMKAYIYDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKK
CIRKGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNEPFAALAFKVATDPFVGR
LTYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEIDSISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFA
EPVISLSVEPKTKADQEKMSLALSKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRE
TFTEVADAEGKYIKQSGGRGQYGHVWIKFEPNHDKGFEFVDNIVGGKVPKEYIKEVENGLIEALTSGPIAGYQTIDVKAT
IFDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDVTTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAK
VPLSEMFGYATDLRSNTQGRGQYIMQFSHYAQAPKSVTEEVMVARAKK

Sequences:

>Translated_688_residues
MSKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMD*MAQEKERGITITSAATYAK*KGHSLNLIDT
PGHVDFTVEVERSLRVLDGAVAVLDGQNGVEPQTETV*RQATKYNVPRIVFVNKMDKTGADFYYSIETMKNRLGVKATAI
QIPIGAEADFVGSIDLIEMKAYIYDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKK
CIRKGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNEPFAALAFKVATDPFVGR
LTYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEIDSISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFA
EPVISLSVEPKTKADQEKMSLALSKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRE
TFTEVADAEGKYIKQSGGRGQYGHV*IKFEPNHDKGFEFVDNIVGGKVPKEYIKEVENGLIEALTSGPIAGYQTIDVKAT
IFDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDVTTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAK
VPLSEMFGYATDLRSNTQGRGQYIMQFSHYAQAPKSVTEEVMVARAKK
>Mature_687_residues
SKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMD*MAQEKERGITITSAATYAK*KGHSLNLIDTP
GHVDFTVEVERSLRVLDGAVAVLDGQNGVEPQTETV*RQATKYNVPRIVFVNKMDKTGADFYYSIETMKNRLGVKATAIQ
IPIGAEADFVGSIDLIEMKAYIYDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKKC
IRKGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNEPFAALAFKVATDPFVGRL
TYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEIDSISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFAE
PVISLSVEPKTKADQEKMSLALSKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRET
FTEVADAEGKYIKQSGGRGQYGHV*IKFEPNHDKGFEFVDNIVGGKVPKEYIKEVENGLIEALTSGPIAGYQTIDVKATI
FDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDVTTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAKV
PLSEMFGYATDLRSNTQGRGQYIMQFSHYAQAPKSVTEEVMVARAKK

Specific function: Catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and

COG id: COG0480

COG function: function code J; Translation elongation factors (GTPases)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-G/EF-2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=688, Percent_Identity=41.1337209302326, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI19923640, Length=706, Percent_Identity=37.5354107648725, Blast_Score=432, Evalue=1e-121,
Organism=Homo sapiens, GI25306283, Length=444, Percent_Identity=40.990990990991, Blast_Score=301, Evalue=2e-81,
Organism=Homo sapiens, GI25306287, Length=283, Percent_Identity=48.4098939929329, Blast_Score=248, Evalue=2e-65,
Organism=Homo sapiens, GI157426893, Length=542, Percent_Identity=25.4612546125461, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI4503483, Length=146, Percent_Identity=39.041095890411, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI94966754, Length=143, Percent_Identity=36.3636363636364, Blast_Score=87, Evalue=5e-17,
Organism=Homo sapiens, GI94966752, Length=88, Percent_Identity=36.3636363636364, Blast_Score=72, Evalue=2e-12,
Organism=Homo sapiens, GI310132016, Length=124, Percent_Identity=34.6774193548387, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=124, Percent_Identity=34.6774193548387, Blast_Score=71, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=124, Percent_Identity=34.6774193548387, Blast_Score=71, Evalue=3e-12,
Organism=Escherichia coli, GI1789738, Length=695, Percent_Identity=57.841726618705, Blast_Score=810, Evalue=0.0,
Organism=Escherichia coli, GI1790835, Length=500, Percent_Identity=27, Blast_Score=149, Evalue=5e-37,
Organism=Escherichia coli, GI48994988, Length=484, Percent_Identity=26.0330578512397, Blast_Score=112, Evalue=1e-25,
Organism=Escherichia coli, GI1788922, Length=159, Percent_Identity=39.622641509434, Blast_Score=95, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=682, Percent_Identity=39.7360703812317, Blast_Score=489, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=714, Percent_Identity=28.4313725490196, Blast_Score=276, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI17506493, Length=441, Percent_Identity=25.8503401360544, Blast_Score=109, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=146, Percent_Identity=42.4657534246575, Blast_Score=100, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17552882, Length=135, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=7e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=692, Percent_Identity=43.2080924855491, Blast_Score=551, Evalue=1e-157,
Organism=Saccharomyces cerevisiae, GI6322359, Length=775, Percent_Identity=31.8709677419355, Blast_Score=355, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6324707, Length=815, Percent_Identity=24.6625766871166, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6320593, Length=815, Percent_Identity=24.6625766871166, Blast_Score=171, Evalue=5e-43,
Organism=Saccharomyces cerevisiae, GI6323320, Length=143, Percent_Identity=38.4615384615385, Blast_Score=81, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324166, Length=145, Percent_Identity=36.551724137931, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=695, Percent_Identity=41.1510791366906, Blast_Score=542, Evalue=1e-154,
Organism=Drosophila melanogaster, GI221458488, Length=727, Percent_Identity=32.4621733149931, Blast_Score=341, Evalue=8e-94,
Organism=Drosophila melanogaster, GI78706572, Length=152, Percent_Identity=38.8157894736842, Blast_Score=92, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24585711, Length=151, Percent_Identity=39.7350993377483, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585713, Length=151, Percent_Identity=39.7350993377483, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24585709, Length=150, Percent_Identity=40, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28574573, Length=139, Percent_Identity=35.2517985611511, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=30.3703703703704, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2520 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 7984 Molecules/Cell In: Growth Phase, Gl

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR000795
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR005225
- InterPro:   IPR004540
- InterPro:   IPR000640
- InterPro:   IPR005517
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF03764 EFG_IV; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 75614; Mature: 75483

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMDMAQEKERGITI
CCCHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEE
TSAATYAKKGHSLNLIDTPGHVDFTVEVERSLRVLDGAVAVLDGQNGVEPQTETVRQATK
EEHHHHHHCCCEEEEEECCCCEEEEEEHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHH
YNVPRIVFVNKMDKTGADFYYSIETMKNRLGVKATAIQIPIGAEADFVGSIDLIEMKAYI
CCCCEEEEEECCCCCCCCEEEEHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEEEEEE
YDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKKCIR
ECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNE
CCCCCCCHHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PFAALAFKVATDPFVGRLTYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEID
CHHHEEEEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHC
SISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFAEPVISLSVEPKTKADQEKMSLAL
CCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
SKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRETFT
HHHHCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHH
EVADAEGKYIKQSGGRGQYGHVIKFEPNHDKGFEFVDNIVGGKVPKEYIKEVENGLIEAL
HHHCCCCCEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TSGPIAGYQTIDVKATIFDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDV
HCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEC
TTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAKVPLSEMFGYATDLRSNTQGRGQYI
CCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCHHH
MQFSHYAQAPKSVTEEVMVARAKK
HHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKELKLFRNFGIMAHIDAGKTTTSERILYHTGKNHKIGETHDGAATMDMAQEKERGITI
CCHHHHHHCCCCEEEECCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCEEE
TSAATYAKKGHSLNLIDTPGHVDFTVEVERSLRVLDGAVAVLDGQNGVEPQTETVRQATK
EEHHHHHHCCCEEEEEECCCCEEEEEEHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHH
YNVPRIVFVNKMDKTGADFYYSIETMKNRLGVKATAIQIPIGAEADFVGSIDLIEMKAYI
CCCCEEEEEECCCCCCCCEEEEHHHHHHHCCCEEEEEEEECCCCCCCCCCEEEEEEEEEE
YDGKADEEYKIEDIPADYLTKAQVMRSQMIDDVAVFDDEVMEKYLSGEELSHEDIKKCIR
ECCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHH
KGVISTELYPVLCGTAFKNKGVKKLLDAVVDFLPSPIDVPPIKGVDDHGNPIEYHNDPNE
CCCCCCCHHHHHHCHHHHCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PFAALAFKVATDPFVGRLTYIRVYSGKLDKGTYIYNATKDKKERISRLVKMHSNNRDEID
CHHHEEEEECCCCCCCCEEEEEEECCCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCHHC
SISAGDICAVIGLKDTTTGDTICDEKKPVILEQMVFAEPVISLSVEPKTKADQEKMSLAL
CCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHH
SKLAEEDPTFRTYTNEETGQTIIAGMGELHLDVLVDRMRREFNVQVNVGAPQVSYRETFT
HHHHCCCCCEEEECCCCCCCEEECCCCHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHH
EVADAEGKYIKQSGGRGQYGHVIKFEPNHDKGFEFVDNIVGGKVPKEYIKEVENGLIEAL
HHHCCCCCEECCCCCCCCCCCEEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHH
TSGPIAGYQTIDVKATIFDGSYHDVDSSGMAYKIAASLAFKEAAKVCKPVLLEPIMSVDV
HCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHEEC
TTPDDYFGTVMGDISKRRGVIEGQEQRGNAQAIKAKVPLSEMFGYATDLRSNTQGRGQYI
CCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEEEECCHHHHHHHHHHHCCCCCCCCHHH
MQFSHYAQAPKSVTEEVMVARAKK
HHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA