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The map label for this gene is ampL

Identifier: 13358070

GI number: 13358070

Start: 625203

End: 626570

Strand: Reverse

Name: ampL

Synonym: UU507

Alternate gene names: 13358070

Gene position: 626570-625203 (Counterclockwise)

Preceding gene: 13358071

Following gene: 13358066

Centisome position: 83.35

GC content: 29.82

Gene sequence:

>1368_bases
ATGACATTAAATGAAAAAAAAGAATTTGTTTATGAATTAAAAGCTGTTGAAAAAAATCACAACCTAAAAGGTTTTGAAGA
AAATACAACTGAACATGTTGTTTTTGGAGGTTTATATCAAAAACAACAATACTTAATTTTAACACCAGATTTTGATTCTA
ATGAACTATCAAGATCATTGATTAGTTTTTTAGAAAAATCACCTAAATCTGTTAGTGTTGATTTAAATTCATTTCTATCA
CTTGTCCCAGAATCAAGACATGCATCTTTATTAAATGTTGTTATTAGTGCATTAGAATATGTTGAAGTAACGCCATTTTC
ACTAAAATCTAAAATTGAAACAAAAAATATACATAATTTAGTAGTTGATCAAAAATATCATGATTTAATTAATAAATTAC
ATGTAATTGCTCAATCACAAACAATTACAAGAACATTACAAGATACGCCAGCTAATTTAATGACACCAGGTGATTTTGAA
GAACGAATTAAAGAATTATTTAAAGACTTGCCAGAAGTTAAAGTAAGTGTTTTATATCGTAAAGATTTAGAAGCTAAAGG
AATGAACGCTCATGTTGGTGTTGGTAAAGCTGCAGTAAGTGATAAAGCACAGCCTCGTTTAGTTGTTGTTGAATATAATA
ATAATCCAGAAACAAATGAAAAATATGCTTTTGTTGGTAAGGGTGTATGTTTTGATTCAGGTGGATATAATGTAAAAACG
GGATCACATATGCGTTGAATGAAATTTGATATGTCTGGTTCTGCTATTGTAAGTATGACAGTGAGAGCTTTAGCTTTAAA
TAAAGAAAAAGTTAATGTTGTGGCTGTATGTCCATTAGTATTAAATTTATTAGCTCCAGAAGGTCAAAAACCAGATGATA
TAGTGAAATCATATAATGGTAAAACAATTGAATTAGATAACACTGATGCAGAAGGACGTTTAATTTTAGCAGATGCACTA
ACATATGCTGTTCGTGATTTAAAAGCTTCTAAATTATTTGATGTTGCAACTTTAACAGGTGCTATGATTTATGCTTTAGG
TGATACATATTCTGGTGTATGAGCAACAAATAACGACATCTGAAATGAAGTAGTTGTAGCAGCTGATTATGCTGGTGAAT
TAGTTTGAAGATTACCATTCCACAATGATTTCTTAAAAATGTTAAATTCAAATGTTGCTGATATTGCTAACTCAGTATCT
GATCCTCGTGGTGGTTCTTCACGTGCAGCATGTTTCTTAAAAGAATTTACTGAAGGCGTTCCATATGCTCATTTTGACAT
TGCTATTACTGCAGATGTTGGTCATAAAGGAACTGGTGTAATGTTAAGAACATTTTATCGTATTGCTCAAAATCAAAAAT
TTAATTAG

Upstream 100 bases:

>100_bases
ATATTAAAACTAACAAGGCACATAATTTAGATAAAAGCATTATAATAATATAAGAATATTAAAATTAAAACTATCAATAT
TAAAGAAGGAACGGAATTTT

Downstream 100 bases:

>100_bases
TTTAAATTTAGATAAAGAAAAAGAAGTGAATTTAATTCGCTTCTTTTTTATTTGTATCATAATTTACGAGTGTCTACGTA
TAATTTTGTAGAAGAAGAGG

Product: leucyl aminopeptidase

Products: NA

Alternate protein names: Leucine aminopeptidase; LAP; Leucyl aminopeptidase [H]

Number of amino acids: Translated: 455; Mature: 454

Protein sequence:

>455_residues
MTLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSLISFLEKSPKSVSVDLNSFLS
LVPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNLVVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFE
ERIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKT
GSHMRWMKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGKTIELDNTDAEGRLILADAL
TYAVRDLKASKLFDVATLTGAMIYALGDTYSGVWATNNDIWNEVVVAADYAGELVWRLPFHNDFLKMLNSNVADIANSVS
DPRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN

Sequences:

>Translated_455_residues
MTLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSLISFLEKSPKSVSVDLNSFLS
LVPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNLVVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFE
ERIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKT
GSHMR*MKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGKTIELDNTDAEGRLILADAL
TYAVRDLKASKLFDVATLTGAMIYALGDTYSGV*ATNNDI*NEVVVAADYAGELV*RLPFHNDFLKMLNSNVADIANSVS
DPRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN
>Mature_454_residues
TLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSLISFLEKSPKSVSVDLNSFLSL
VPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNLVVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFEE
RIKELFKDLPEVKVSVLYRKDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKTG
SHMR*MKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGKTIELDNTDAEGRLILADALT
YAVRDLKASKLFDVATLTGAMIYALGDTYSGV*ATNNDI*NEVVVAADYAGELV*RLPFHNDFLKMLNSNVADIANSVSD
PRGGSSRAACFLKEFTEGVPYAHFDIAITADVGHKGTGVMLRTFYRIAQNQKFN

Specific function: Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides [H]

COG id: COG0260

COG function: function code E; Leucyl aminopeptidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M17 family [H]

Homologues:

Organism=Homo sapiens, GI41393561, Length=298, Percent_Identity=38.9261744966443, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI47155554, Length=317, Percent_Identity=36.2776025236593, Blast_Score=154, Evalue=2e-37,
Organism=Escherichia coli, GI1790710, Length=307, Percent_Identity=34.2019543973941, Blast_Score=166, Evalue=3e-42,
Organism=Escherichia coli, GI87082123, Length=321, Percent_Identity=33.3333333333333, Blast_Score=119, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17556903, Length=343, Percent_Identity=37.0262390670554, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17565172, Length=214, Percent_Identity=35.0467289719626, Blast_Score=99, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24661038, Length=291, Percent_Identity=33.6769759450172, Blast_Score=147, Evalue=1e-35,
Organism=Drosophila melanogaster, GI21355725, Length=291, Percent_Identity=33.3333333333333, Blast_Score=146, Evalue=3e-35,
Organism=Drosophila melanogaster, GI21357381, Length=317, Percent_Identity=33.1230283911672, Blast_Score=146, Evalue=4e-35,
Organism=Drosophila melanogaster, GI221379063, Length=317, Percent_Identity=33.1230283911672, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI221379062, Length=317, Percent_Identity=33.1230283911672, Blast_Score=145, Evalue=5e-35,
Organism=Drosophila melanogaster, GI20129969, Length=370, Percent_Identity=25.1351351351351, Blast_Score=112, Evalue=7e-25,
Organism=Drosophila melanogaster, GI24662227, Length=311, Percent_Identity=28.2958199356913, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI161077148, Length=305, Percent_Identity=27.8688524590164, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI20130057, Length=305, Percent_Identity=27.8688524590164, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI19922386, Length=301, Percent_Identity=28.2392026578073, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129963, Length=292, Percent_Identity=29.7945205479452, Blast_Score=99, Evalue=4e-21,
Organism=Drosophila melanogaster, GI24646701, Length=374, Percent_Identity=26.7379679144385, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24646703, Length=374, Percent_Identity=26.7379679144385, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21358201, Length=374, Percent_Identity=26.7379679144385, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21355645, Length=291, Percent_Identity=25.4295532646048, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24662223, Length=291, Percent_Identity=25.4295532646048, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011356
- InterPro:   IPR000819
- InterPro:   IPR023042 [H]

Pfam domain/function: PF00883 Peptidase_M17 [H]

EC number: =3.4.11.1; =3.4.11.10 [H]

Molecular weight: Translated: 49812; Mature: 49681

Theoretical pI: Translated: 6.45; Mature: 6.45

Prosite motif: PS00631 CYTOSOL_AP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSL
CCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEECCCEEEEECCCCCCHHHHHHH
ISFLEKSPKSVSVDLNSFLSLVPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNL
HHHHHCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHH
VVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFEERIKELFKDLPEVKVSVLYR
HHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHEEEHHEE
KDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKT
CCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCEEECCCCCEECC
GSHMRMKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGK
CCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHCCCC
TIELDNTDAEGRLILADALTYAVRDLKASKLFDVATLTGAMIYALGDTYSGVATNNDINE
EEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCE
VVVAADYAGELVRLPFHNDFLKMLNSNVADIANSVSDPRGGSSRAACFLKEFTEGVPYAH
EEEEECCCCCEEECCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEE
FDIAITADVGHKGTGVMLRTFYRIAQNQKFN
EEEEEEECCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
TLNEKKEFVYELKAVEKNHNLKGFEENTTEHVVFGGLYQKQQYLILTPDFDSNELSRSL
CCCHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCEECCCEEEEECCCCCCHHHHHHH
ISFLEKSPKSVSVDLNSFLSLVPESRHASLLNVVISALEYVEVTPFSLKSKIETKNIHNL
HHHHHCCCCEEEEEHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHH
VVDQKYHDLINKLHVIAQSQTITRTLQDTPANLMTPGDFEERIKELFKDLPEVKVSVLYR
HHCHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCHHHEEEHHEE
KDLEAKGMNAHVGVGKAAVSDKAQPRLVVVEYNNNPETNEKYAFVGKGVCFDSGGYNVKT
CCCCCCCCCCCCCCCCHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCEEECCCCCEECC
GSHMRMKFDMSGSAIVSMTVRALALNKEKVNVVAVCPLVLNLLAPEGQKPDDIVKSYNGK
CCEEEEEEECCCCHHHHHHHHHHHCCCCCCEEEEEHHHHHHHHCCCCCCCHHHHHHCCCC
TIELDNTDAEGRLILADALTYAVRDLKASKLFDVATLTGAMIYALGDTYSGVATNNDINE
EEEECCCCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCE
VVVAADYAGELVRLPFHNDFLKMLNSNVADIANSVSDPRGGSSRAACFLKEFTEGVPYAH
EEEEECCCCCEEECCCCHHHHHHHCCCHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEE
FDIAITADVGHKGTGVMLRTFYRIAQNQKFN
EEEEEEECCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]