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The map label for this gene is pfs

Identifier: 13358033

GI number: 13358033

Start: 536299

End: 536655

Strand: Direct

Name: pfs

Synonym: UU470

Alternate gene names: 13358033

Gene position: 536299-536655 (Clockwise)

Preceding gene: 13358032

Following gene: 13358034

Centisome position: 71.34

GC content: 25.21

Gene sequence:

>357_bases
ATGATTGGTTTAATTGTGGCATTAAATAGTGAAATTAAAACCTTTTTTAAACAAATTAAAAAGAAGGTTTATCAAATTAA
TAATATTGATTTTTACTTGTGCACTCATCAAAATATAGAATTTGTTTTAGTTTTTACGGATGTAGGAAAAACTAATGCGA
GTTTTATTACTGCATTATTAATTAATAATTTTAAACCTAAAGTGATTTTAAACGTTGGTAGTTGTGGTGCACTAAATGAT
CAGTTACAGGTTTTAGATATTGCCATTATTGATCAATGCCAATACTTAGATGTTAACGTTAGCGCTTTTGGTTATTTAAA
AAATCAAATACCGCGTTTAGATAATTTTTTATTTTAG

Upstream 100 bases:

>100_bases
TAACATTGATTATTATCGAAAATTAGTTTTTGAAGATCTAGATAAGGCATTTATTGAAGTTTATCAATACCAACAAAGAC
AAAGAAAATAAGGTAAAAAT

Downstream 100 bases:

>100_bases
ATAAAAGTTATAATCAACAAATCAAAAACCAGTTAATTAAGAAAAAACTAAAATGTTGAATTGCAAATGTTGGAAGCAGT
GACACATTTATTAATCGTGA

Product: purine nucleoside phosphorylase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase [H]

Number of amino acids: Translated: 118; Mature: 118

Protein sequence:

>118_residues
MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALLINNFKPKVILNVGSCGALND
QLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDNFLF

Sequences:

>Translated_118_residues
MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALLINNFKPKVILNVGSCGALND
QLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDNFLF
>Mature_118_residues
MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALLINNFKPKVILNVGSCGALND
QLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDNFLF

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=111, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 13408; Mature: 13408

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALL
CEEEEEEECHHHHHHHHHHHHHHEEECCEEEEEEECCCEEEEEEEECCCCCCHHHHHEEH
INNFKPKVILNVGSCGALNDQLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDNFLF
HCCCCCEEEEEECCCCCCCCCEEEEEEEEECCEEEEEECHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIGLIVALNSEIKTFFKQIKKKVYQINNIDFYLCTHQNIEFVLVFTDVGKTNASFITALL
CEEEEEEECHHHHHHHHHHHHHHEEECCEEEEEEECCCEEEEEEEECCCCCCHHHHHEEH
INNFKPKVILNVGSCGALNDQLQVLDIAIIDQCQYLDVNVSAFGYLKNQIPRLDNFLF
HCCCCCEEEEEECCCCCCCCCEEEEEEEEECCEEEEEECHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA