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The map label for this gene is polA

Identifier: 13357976

GI number: 13357976

Start: 477118

End: 477987

Strand: Reverse

Name: polA

Synonym: UU414

Alternate gene names: 13357976

Gene position: 477987-477118 (Counterclockwise)

Preceding gene: 13357977

Following gene: 13357975

Centisome position: 63.59

GC content: 26.67

Gene sequence:

>870_bases
ATGAAAAAAGCAATTGTTATTGATGGAAATTCATTGATTTATCGAGCCTTTCACGCTACTTATAAACAAGCTGAATGAGC
AGTTGAAAAACAATTAATGCCTACTAATGCCATTAAATTAGTAGCCTCAATGATTTTTAAGATTTTAAATCAAGAACAAT
TTACTTATGGTTTAATTGCTATGGACGCTTCTAAACAAACTTTTCGTGCTCAAGAATATGCTGCTTACAAAGCGACACGT
AAACCTATGGATGCAAAACTAATTGTTCAATTACCCTATTTAAAAAAACTATTCGTTGCTATGGGTTTTCGAATAATTTC
ACAACCTGGAATTGAAGCTGACGATTTTGTTGGTTCTTTTAGTAATTTAATGAATAAGTTTGATGTTGATACAACTATTT
ATAGTTCAGATCGTGATATGTTGCAATTAATTAACAAAAACACAAAACTTAAACTCTTAAAAACAGGGACAAGTGTTATG
CAAGAAATTAATTTAACAAATTTTTCATTATTAAATAATGGCATTTTACCGTATCAAATTATTGAATATAAGGGTTTAGT
GGGTGATAGTAGTGATAATTTAATTGGCGTGAAGGGAATCGGCCCTAAAACAGCTATTAATTTACTTTTAAAATATGATA
ATATAGAAAATATTTATACTAATTTAGAAAAAATAGCACCTAGTGTTAAAAATAAATTAATTGAACACAAAGATATGGCT
TTTTTATCAAAAAAATTAGCGGCGATTCAAACGGATTTATTGTTTGATGAAAAATTAGAAAATTTTGTTTTAAAACCATA
TAATACTCAAGAATTAGATGAGTTATTCACATCTTTAAAAATAAATAATATGCAAAATTATTATAAATAA

Upstream 100 bases:

>100_bases
TTTAATTGATGAAATTAGTATTGGAGGTATTTATGTTTTTGGAGTTAAATTATTAAATCATTTTAATTTTATTGTAAATA
TTAAGCAAAGGATTTAGATT

Downstream 100 bases:

>100_bases
TTTATCCATATTATAATCTGCTATATAAGGGGGTTTAAAAAATGCCAGAGCTACCAGAAGTACAAACGATTGTTGATTAT
TTAAATCATCATGTTCTCGA

Product: DNA polymerase I

Products: N Pyrophosphate; DNA(N). [C]

Alternate protein names: NA

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MKKAIVIDGNSLIYRAFHATYKQAEWAVEKQLMPTNAIKLVASMIFKILNQEQFTYGLIAMDASKQTFRAQEYAAYKATR
KPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFSNLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVM
QEINLTNFSLLNNGILPYQIIEYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMA
FLSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK

Sequences:

>Translated_289_residues
MKKAIVIDGNSLIYRAFHATYKQAE*AVEKQLMPTNAIKLVASMIFKILNQEQFTYGLIAMDASKQTFRAQEYAAYKATR
KPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFSNLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVM
QEINLTNFSLLNNGILPYQIIEYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMA
FLSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK
>Mature_289_residues
MKKAIVIDGNSLIYRAFHATYKQAE*AVEKQLMPTNAIKLVASMIFKILNQEQFTYGLIAMDASKQTFRAQEYAAYKATR
KPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFSNLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVM
QEINLTNFSLLNNGILPYQIIEYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMA
FLSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK

Specific function: 5'-3' exonuclease acting preferentially on double- stranded DNA [H]

COG id: COG0258

COG function: function code L; 5'-3' exonuclease (including N-terminal domain of PolI)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 5'-3' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1790294, Length=278, Percent_Identity=32.7338129496403, Blast_Score=141, Evalue=5e-35,
Organism=Escherichia coli, GI226510970, Length=233, Percent_Identity=30.9012875536481, Blast_Score=122, Evalue=3e-29,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020046
- InterPro:   IPR020045
- InterPro:   IPR002421
- InterPro:   IPR020047
- InterPro:   IPR008918
- InterPro:   IPR003583 [H]

Pfam domain/function: PF01367 5_3_exonuc; PF02739 5_3_exonuc_N [H]

EC number: 2.7.7.7 [C]

Molecular weight: Translated: 32741; Mature: 32741

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKAIVIDGNSLIYRAFHATYKQAEAVEKQLMPTNAIKLVASMIFKILNQEQFTYGLIAM
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
DASKQTFRAQEYAAYKATRKPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFS
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVMQEINLTNFSLLNNGILPYQII
HHHHHHCCCCEEECCCHHHHHHHCCCCEEEEEECCHHHHHHHCCCHHHHHHCCCCCEEEE
EYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMAF
EECCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCC
>Mature Secondary Structure
MKKAIVIDGNSLIYRAFHATYKQAEAVEKQLMPTNAIKLVASMIFKILNQEQFTYGLIAM
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCEEEEEEE
DASKQTFRAQEYAAYKATRKPMDAKLIVQLPYLKKLFVAMGFRIISQPGIEADDFVGSFS
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
NLMNKFDVDTTIYSSDRDMLQLINKNTKLKLLKTGTSVMQEINLTNFSLLNNGILPYQII
HHHHHHCCCCEEECCCHHHHHHHCCCCEEEEEECCHHHHHHHCCCHHHHHHCCCCCEEEE
EYKGLVGDSSDNLIGVKGIGPKTAINLLLKYDNIENIYTNLEKIAPSVKNKLIEHKDMAF
EECCCCCCCCCCEEEECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSKKLAAIQTDLLFDEKLENFVLKPYNTQELDELFTSLKINNMQNYYK
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: Dimethyl sulfoxide epsilon,; Polymerase alpha accessory factors; Proliferating cell nuclear antigen; Replication factor A; Replication factor C; Thiol [C]

Metal ions: K+; Mg2+; Mn2+; Na+; Zn2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.0063 {dATP}} [C]

Substrates: N Deoxynucleoside Triphosphate; DNA [C]

Specific reaction: N Deoxynucleoside Triphosphate = N Pyrophosphate + DNA(N). Protein + DNA = Protein-DNA [C]

General reaction: Nucleotidyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2', 3'-Dideoxythymidine5'-triphosphate polymerase beta, gamma, delta, epsilon; Aphidicolin polymerase alpha; Ara-ATP; Ara-CTP; Benzyl oxycarbonyl -Leu-Leu-al; Carbonyl diphosphonate delta, alpha,; Dideoxynucleoside 5'-triphosphate;

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11048724 [H]