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The map label for this gene is mraW

Identifier: 13357947

GI number: 13357947

Start: 443585

End: 444511

Strand: Direct

Name: mraW

Synonym: UU386

Alternate gene names: 13357947

Gene position: 443585-444511 (Clockwise)

Preceding gene: 13357946

Following gene: 13357948

Centisome position: 59.01

GC content: 24.6

Gene sequence:

>927_bases
ATGGAATTTAATCAACACATTACCGTTTTATTAAACGAAACAATCGAATTATTAAATATTAAACCAGATGGCATTTATGT
TGATTGTACATTTGGTCGTGGCGGACATTCACAACTCATCTTAAAAAAACTCTCAAAAAAAGGTAAATTAATTTGTTTAG
ATCAAGATCAAGAAGCAATTAATTTTGCAAATAATTTATTTAAAAATAATACAAATGTAATTGTTATTAAAACAAACTTT
AAAAATTTAAAATCAGCATTAAGTGCACATAAAATTTTTTATGTTGATGGCTTTATCTTTGATTTAGGTTTATCAAGTCC
ACAACTTGATGATCCAAAAAGAGGTTTTAGTTATCATAAGAATGCTTGATTAGATATGCGAATGGATCAATCGCAAAACT
TAAATGCTCACTATATTGTTAATAATTATTCTTTTGCAAAATTAGTAAGTATTTTTAAACGATATGGAGAAATAAAATAT
CCAAAAATTATTGCTGATGCAATTGTTAAAGAACGCAGTATTAAAGAGATTAATACAACATTGGAATTAGTTGAAATCAT
TAAAAAATATTCTCCTAAAAAAAATTTATTTGAAAAAAAACATCCTGCACGTTTATTTTTTCAAGCAATTAGAATAGAAG
TTAATGATGAATTAAACATTTTAGAAAAAGCATTTAATGATGCTATTTCAATGTTAAATCCCTTAGGTGTTGTGGCAATT
ATTAGCTTTCATAGTCTTGAAGATAAAATTGTTAAAAAGGTCTTTAATAATTATGCAAAAATTAAATTACCTAAAGAAGT
CCCAATAAATAATTATGTAAATAAATATAGTTTATTGAATCAAAAAATTATGCCAAGCACCCAGGAACTAAATGATAACA
ATCGTTCACGATCATCTATTTTAAGGGGATTAATTAGAAATTATTAA

Upstream 100 bases:

>100_bases
ACAAATGATCGATATGAAATTTGGGCTAAAGAAGTTTATGATGATTTTAGCTTAAATCAAGAAAGTACGCTAAGTGATTT
AGCTGAAAAATTATTAATTA

Downstream 100 bases:

>100_bases
AAATGGAGGATTTTATTTATGAACAAACCAATGTGTTTGCTTCGTTTAGATAATTATTATTTTACATTGTTGGTATTTAT
TTATAATGATGTAACTAAAG

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNF
KNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNAWLDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKY
PKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAI
ISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY

Sequences:

>Translated_308_residues
MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNF
KNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNA*LDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKY
PKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAI
ISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY
>Mature_308_residues
MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAINFANNLFKNNTNVIVIKTNF
KNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHKNA*LDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKY
PKIIADAIVKERSIKEINTTLELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAI
ISFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSILRGLIRNY

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=336, Percent_Identity=34.8214285714286, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI165377202, Length=192, Percent_Identity=38.5416666666667, Blast_Score=110, Evalue=1e-24,
Organism=Escherichia coli, GI1786270, Length=309, Percent_Identity=41.1003236245955, Blast_Score=235, Evalue=4e-63,
Organism=Drosophila melanogaster, GI62472493, Length=331, Percent_Identity=34.1389728096677, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI28571637, Length=331, Percent_Identity=34.1389728096677, Blast_Score=149, Evalue=3e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35354; Mature: 35354

Theoretical pI: Translated: 10.07; Mature: 10.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAI
CCCCCHHHHEECCCEEHEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHH
NFANNLFKNNTNVIVIKTNFKNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHK
HHHHHHHCCCCCEEEEEECHHHHHHHHHHCEEEEEECEEEECCCCCCCCCCCCCCHHHHH
NALDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKYPKIIADAIVKERSIKEINTTL
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAII
HHHHHHHHCCCCHHHHHHCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCHHHHHHHH
SFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSIL
HHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHH
RGLIRNY
HHHHHCC
>Mature Secondary Structure
MEFNQHITVLLNETIELLNIKPDGIYVDCTFGRGGHSQLILKKLSKKGKLICLDQDQEAI
CCCCCHHHHEECCCEEHEEECCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEECCCHHHH
NFANNLFKNNTNVIVIKTNFKNLKSALSAHKIFYVDGFIFDLGLSSPQLDDPKRGFSYHK
HHHHHHHCCCCCEEEEEECHHHHHHHHHHCEEEEEECEEEECCCCCCCCCCCCCCHHHHH
NALDMRMDQSQNLNAHYIVNNYSFAKLVSIFKRYGEIKYPKIIADAIVKERSIKEINTTL
HHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
ELVEIIKKYSPKKNLFEKKHPARLFFQAIRIEVNDELNILEKAFNDAISMLNPLGVVAII
HHHHHHHHCCCCHHHHHHCCHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHCHHHHHHHH
SFHSLEDKIVKKVFNNYAKIKLPKEVPINNYVNKYSLLNQKIMPSTQELNDNNRSRSSIL
HHHHHHHHHHHHHHCCHHEEECCCCCCCHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHH
RGLIRNY
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA