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The map label for this gene is panB

Identifier: 126696834

GI number: 126696834

Start: 1266131

End: 1266904

Strand: Direct

Name: panB

Synonym: P9301_14961

Alternate gene names: 126696834

Gene position: 1266131-1266904 (Clockwise)

Preceding gene: 126696833

Following gene: 126696836

Centisome position: 77.11

GC content: 35.01

Gene sequence:

>774_bases
ATGTTACCTTCAGACCTAGTTAATTATAAAAAAAAATCTCGCAAAATCATTGCACTAACTGCTTGGGACTCCATATCAGG
ATCTATTGCAGAACAAGCAAATGTTGATCTCGTACTAGTAGGAGATTCATTAGCAATGGTCTGCTTAGGATACCAATCAA
CATTGCCAATAACTTTAGAAAATATAATTTATCATACTAATGCTGTTTCAAGAGGATTTAAGAAAAAAATTGAGGAACAA
CCTCTAGTCGTTTCAGATATGCCTTTTATGACTTACCAATGTGGCGAGGATAAGGCTGTTGAGTATGCAGGGAAAATCAT
TCAGAGCACTTATGCAAAAGCTGTAAAAGTGGAAGGAGCTGAACCAGAAATACAAAAAGTTATTTCTAGATTAATAAGAA
TGGGAATCCCTGTTATGGGTCATATAGGTCTTACACCACAAAGCTATCTAAATATTGGATTAAGAAAACAAGGAGAAAGC
TTGGCAAGCCAAGAAAAAATTAAGAAAGAGGCTTCAATTCTTGAAGAATTAGGATGTTTTTCAATAGTTCTTGAACATAT
TCCTGATTTGCTTGCTAAGGAAATACAAAATTCATTAACAATTCCCACAATAGGTATCGGCGCAGGTAATTATTGCGATG
GGCAAGTAAGAGTTACAGCAGATTTGCTAGGCCTCAATGATGATCAACCCCCATTTTGCCAACCAATTATCCAAGGAAAG
AAATTATTTAAGGATAAATTAAAAGAATGGGTAGACTCTGAAAGGCTTAGTTAA

Upstream 100 bases:

>100_bases
CTACCTTCCGGTCCTGACCAGGTTTAGGCGTTAAAACCGCGAAAGGCCGAGTCAAAATTATATTAGCAATTCTTGATTAA
AAAAGATACATAAAATTATT

Downstream 100 bases:

>100_bases
TCTCATCCCACCACTTAAACATAGAGACAAGAATTTGATTACTAAGTTCCATGCCATTAGGCTCACTTAAAAAGAATCTA
TTCCCCTTTCTTACTAGTAG

Product: 3-methyl-2-oxobutanoate hydroxymethyltransferase

Products: NA

Alternate protein names: Ketopantoate hydroxymethyltransferase; KPHMT

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MLPSDLVNYKKKSRKIIALTAWDSISGSIAEQANVDLVLVGDSLAMVCLGYQSTLPITLENIIYHTNAVSRGFKKKIEEQ
PLVVSDMPFMTYQCGEDKAVEYAGKIIQSTYAKAVKVEGAEPEIQKVISRLIRMGIPVMGHIGLTPQSYLNIGLRKQGES
LASQEKIKKEASILEELGCFSIVLEHIPDLLAKEIQNSLTIPTIGIGAGNYCDGQVRVTADLLGLNDDQPPFCQPIIQGK
KLFKDKLKEWVDSERLS

Sequences:

>Translated_257_residues
MLPSDLVNYKKKSRKIIALTAWDSISGSIAEQANVDLVLVGDSLAMVCLGYQSTLPITLENIIYHTNAVSRGFKKKIEEQ
PLVVSDMPFMTYQCGEDKAVEYAGKIIQSTYAKAVKVEGAEPEIQKVISRLIRMGIPVMGHIGLTPQSYLNIGLRKQGES
LASQEKIKKEASILEELGCFSIVLEHIPDLLAKEIQNSLTIPTIGIGAGNYCDGQVRVTADLLGLNDDQPPFCQPIIQGK
KLFKDKLKEWVDSERLS
>Mature_257_residues
MLPSDLVNYKKKSRKIIALTAWDSISGSIAEQANVDLVLVGDSLAMVCLGYQSTLPITLENIIYHTNAVSRGFKKKIEEQ
PLVVSDMPFMTYQCGEDKAVEYAGKIIQSTYAKAVKVEGAEPEIQKVISRLIRMGIPVMGHIGLTPQSYLNIGLRKQGES
LASQEKIKKEASILEELGCFSIVLEHIPDLLAKEIQNSLTIPTIGIGAGNYCDGQVRVTADLLGLNDDQPPFCQPIIQGK
KLFKDKLKEWVDSERLS

Specific function: Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is tranferred onto alpha-ketoisovalerate to form ketopantoate

COG id: COG0413

COG function: function code H; Ketopantoate hydroxymethyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the panB family

Homologues:

Organism=Escherichia coli, GI1786326, Length=230, Percent_Identity=39.1304347826087, Blast_Score=145, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6319653, Length=242, Percent_Identity=40.0826446280992, Blast_Score=158, Evalue=1e-39,

Paralogues:

None

Copy number: 1080 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PANB_PROM0 (A3PEE4)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091720.1
- ProteinModelPortal:   A3PEE4
- SMR:   A3PEE4
- STRING:   A3PEE4
- GeneID:   4912833
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_14961
- eggNOG:   COG0413
- HOGENOM:   HBG299908
- OMA:   YATPEQT
- ProtClustDB:   PRK00311
- BioCyc:   PMAR167546:P9301ORF_1523-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00156
- InterPro:   IPR003700
- InterPro:   IPR015813
- Gene3D:   G3DSA:3.20.20.60
- PANTHER:   PTHR20881
- PIRSF:   PIRSF000388
- TIGRFAMs:   TIGR00222

Pfam domain/function: PF02548 Pantoate_transf; SSF51621 Pyrv/PenolPyrv_Kinase_cat

EC number: =2.1.2.11

Molecular weight: Translated: 28321; Mature: 28321

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: ACT_SITE 185-185 BINDING 86-86 BINDING 116-116

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPSDLVNYKKKSRKIIALTAWDSISGSIAEQANVDLVLVGDSLAMVCLGYQSTLPITLE
CCCHHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCEEEECCCHHHHHHCCCCCCCEEHH
NIIYHTNAVSRGFKKKIEEQPLVVSDMPFMTYQCGEDKAVEYAGKIIQSTYAKAVKVEGA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCC
EPEIQKVISRLIRMGIPVMGHIGLTPQSYLNIGLRKQGESLASQEKIKKEASILEELGCF
CHHHHHHHHHHHHHCCCCEECCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
SIVLEHIPDLLAKEIQNSLTIPTIGIGAGNYCDGQVRVTADLLGLNDDQPPFCQPIIQGK
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
KLFKDKLKEWVDSERLS
HHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLPSDLVNYKKKSRKIIALTAWDSISGSIAEQANVDLVLVGDSLAMVCLGYQSTLPITLE
CCCHHHHHHHHHCCEEEEEEECCCCCCCHHHHCCCCEEEECCCHHHHHHCCCCCCCEEHH
NIIYHTNAVSRGFKKKIEEQPLVVSDMPFMTYQCGEDKAVEYAGKIIQSTYAKAVKVEGA
HHHHHHHHHHHHHHHHHHCCCEEEECCCCEEEECCCCHHHHHHHHHHHHHHHHHEECCCC
EPEIQKVISRLIRMGIPVMGHIGLTPQSYLNIGLRKQGESLASQEKIKKEASILEELGCF
CHHHHHHHHHHHHHCCCCEECCCCCHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHHHHHH
SIVLEHIPDLLAKEIQNSLTIPTIGIGAGNYCDGQVRVTADLLGLNDDQPPFCQPIIQGK
HHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCCEEEEEEEEECCCCCCCCHHHHHHHHH
KLFKDKLKEWVDSERLS
HHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA