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The map label for this gene is hdhA [C]

Identifier: 126696777

GI number: 126696777

Start: 1209844

End: 1210551

Strand: Reverse

Name: hdhA [C]

Synonym: P9301_14391

Alternate gene names: 126696777

Gene position: 1210551-1209844 (Counterclockwise)

Preceding gene: 126696778

Following gene: 126696775

Centisome position: 73.73

GC content: 27.82

Gene sequence:

>708_bases
GTGGAAGACCTAAAAAAAGTACTTATAGTAGGTGTTAATTCTAAAATTGGCTCGGCAGTTCTTGATCTTTATTGGAAACA
AAAATATATTTGTTATGGAACATACAATAAAAATGCTCCGCCTAAGGATGTAAAAAATAAATGCAATAATATTTTAAGAT
GTGATTTAACGCAACTTTTTGGAATAAATGATTTACTAGCTTTTAGCAATAAATTTAAACCTTACAATATTATTTATTTA
CCAGGATATGTGGACAATAAAAGTTTATCTAAAAATGATCTACAAAGTATGCATAATTCTTTTAATGTAAATGTCTTTGC
ATATTGGTTATTAATTAGTGGTTGTATTCCTTATATGAAAAAAAAAGGGTTTGGAAGGTTTTTGTCTTTATCATCAATTG
GATCTAAATTTGGAGGTGGTGAAGATAAATTCAACTATACAACTAGTAAAAGAATGCTTGAGTTTTTCCCAAAAGATTTT
AAGAATATTGCAAGAGATAACATATTCTTGAACAATATAATTTGTGGCGCTACGAATACACCAATTTTAAAAAAGAAAAA
TAATGAGTCTATTGATCAAAGAGCAACATTGATTCCAGTAGGAAGATTGGCTGACCCAAAAGAAATTGCGAAATGTTGTT
ATCAAATATGTTCTTTAGATAATACTTTTCAAACTCTTTCAAATATCACAATAGCAGGTGGTGAATAA

Upstream 100 bases:

>100_bases
ACTTACTTGAGTCGAAAAGGGAAGTTTCTGAAGCATGCAAAAAATGTGATGTACCTGGGGATTTCATGGGTTTAGAACAA
GCCAAAGAATGGAGGAAGTT

Downstream 100 bases:

>100_bases
CTAAAGTTTTATAGTTTGGAATAAGTTGGTTTTATTTTGTTACTATTATGAAAATAATTTTTAAATTTAATTACTTAATT
GAAGTTGAATAAAAAATAAA

Product: hypothetical protein

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: 3-Oxoacyl-[Acyl-Carrier-Protein] Reductase; Short-Chain Dehydrogenase/Reductase SDR; Acetyoacetyl CoA Reductase; Short-Chain Dehydrogenase/Reductase Family Oxidoreductase; Acetoacetyl-CoA Reductase; 3-Oxoacyl-[Acyl Carrier Protein] Reductase

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MEDLKKVLIVGVNSKIGSAVLDLYWKQKYICYGTYNKNAPPKDVKNKCNNILRCDLTQLFGINDLLAFSNKFKPYNIIYL
PGYVDNKSLSKNDLQSMHNSFNVNVFAYWLLISGCIPYMKKKGFGRFLSLSSIGSKFGGGEDKFNYTTSKRMLEFFPKDF
KNIARDNIFLNNIICGATNTPILKKKNNESIDQRATLIPVGRLADPKEIAKCCYQICSLDNTFQTLSNITIAGGE

Sequences:

>Translated_235_residues
MEDLKKVLIVGVNSKIGSAVLDLYWKQKYICYGTYNKNAPPKDVKNKCNNILRCDLTQLFGINDLLAFSNKFKPYNIIYL
PGYVDNKSLSKNDLQSMHNSFNVNVFAYWLLISGCIPYMKKKGFGRFLSLSSIGSKFGGGEDKFNYTTSKRMLEFFPKDF
KNIARDNIFLNNIICGATNTPILKKKNNESIDQRATLIPVGRLADPKEIAKCCYQICSLDNTFQTLSNITIAGGE
>Mature_235_residues
MEDLKKVLIVGVNSKIGSAVLDLYWKQKYICYGTYNKNAPPKDVKNKCNNILRCDLTQLFGINDLLAFSNKFKPYNIIYL
PGYVDNKSLSKNDLQSMHNSFNVNVFAYWLLISGCIPYMKKKGFGRFLSLSSIGSKFGGGEDKFNYTTSKRMLEFFPKDF
KNIARDNIFLNNIICGATNTPILKKKNNESIDQRATLIPVGRLADPKEIAKCCYQICSLDNTFQTLSNITIAGGE

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 26554; Mature: 26554

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEDLKKVLIVGVNSKIGSAVLDLYWKQKYICYGTYNKNAPPKDVKNKCNNILRCDLTQLF
CCCHHEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHEEEECHHHHH
GINDLLAFSNKFKPYNIIYLPGYVDNKSLSKNDLQSMHNSFNVNVFAYWLLISGCIPYMK
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KKGFGRFLSLSSIGSKFGGGEDKFNYTTSKRMLEFFPKDFKNIARDNIFLNNIICGATNT
HCCCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCC
PILKKKNNESIDQRATLIPVGRLADPKEIAKCCYQICSLDNTFQTLSNITIAGGE
CCEECCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCEEECCCC
>Mature Secondary Structure
MEDLKKVLIVGVNSKIGSAVLDLYWKQKYICYGTYNKNAPPKDVKNKCNNILRCDLTQLF
CCCHHEEEEEECCCHHHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHHHEEEECHHHHH
GINDLLAFSNKFKPYNIIYLPGYVDNKSLSKNDLQSMHNSFNVNVFAYWLLISGCIPYMK
CHHHHHHHCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KKGFGRFLSLSSIGSKFGGGEDKFNYTTSKRMLEFFPKDFKNIARDNIFLNNIICGATNT
HCCCCCEEEHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEEEECCCCC
PILKKKNNESIDQRATLIPVGRLADPKEIAKCCYQICSLDNTFQTLSNITIAGGE
CCEECCCCCCHHHHHEEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHCEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA