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The map label for this gene is sdhA [C]

Identifier: 126696603

GI number: 126696603

Start: 1075597

End: 1077309

Strand: Reverse

Name: sdhA [C]

Synonym: P9301_12651

Alternate gene names: 126696603

Gene position: 1077309-1075597 (Counterclockwise)

Preceding gene: 126696606

Following gene: 126696600

Centisome position: 65.61

GC content: 33.92

Gene sequence:

>1713_bases
GTGATTGGATGCGGTGGAGCTGGTTTAAGATCTGCTATTGAAATAAAAAAATCTGGTCTCGATGTATGTATTCTAGGAAA
AAGACCGAAGACTGATGCTCATACCGTCTTAGCGGCAGGAGGCATAAATGCTGCTTTAGGCAACTTAGATAAAGAAGATT
CATGGGAACAGCATTTTATTGATACTTATTTAGAGGGTTATGGCATTGGGGATCCTTTAAAGGTTGAAATAATGGCTAAA
GAATCACCATCACTAGTAAAAGAAATAGATAAATGGGGAGCTAACTTTGCAAAATTAAAAAATGGTGAACTAGATCAAAG
ATTTTTTGGTGCGCACAAATATCGCAGAACTTGTTATTCAGGTGATTTTACTGGATTATCAATTTTAAAAACTCTCTTAA
AAAAATCAGATGATTTAAAGATTCCCATCTATGACAATCAATATGTAACTGAATTATTGATTAGGGAAAATACATGCTTT
GGAGCAATGTCATTTAATTTGTCTACTTCAGAGAGGACTGTGCACTTAGCAGATGCGGTTATTTTATGTACAGGAGGGCA
TACAAGATTATGGAAAAAAAGTTCATCTAGAAAAAATGAAAATACAGGTGATGGTTATTATTTAAGTCTAAAAGGAGGAT
GTGAATTAATAGATATGGAAATGGTGCAATTCCACCCTTCTGGAATGGTCTTACCCGAAGAAATTGAGGGTACTTTGGTT
ACTGAAGCGGTAAGAGGTGAGGGTGGAAAGTTAATAAATAATAAAGGCGAAAGATTTATGAGAGAATATGATCCGAAAAG
GATGGAATTATCTACGAGAGACAGAGTAGCAATTGCTAACTATACAGAAATAATTGAAGGCAGAGGAACCAAAAATGGTG
GTGTTTTTCTTGATATAAGCCATAAAAGTAAAGAGTTCATTATTGAAAAACTTCCAAGCATATACAGACAATTTCTTGAA
GCTCAAATGCTTGACATTTCAAAATCACCAATGGAAGTTGCGCCAACTGCTCACTATTCTATGGGCGGAATTTTAGTTAA
TCCTGAGGATTTATCCACTTCTGTAGAGGGTCTTTTTGCGGCTGGTGAAGTTGCAGGAGGACTTCATGGAGCTAATCGTT
TGGGTGGTAATTCGTTAGCAGAAATTATTATTTTTGGAAGACGTGCTGGTATTGCAGCCTCAAAATATTCAAAAAATTTA
GCTCAGCAAATGAGATCAAATAAAGCCATCGCCATTGCCCATCAAAATATTAATAAGTTTATTAAAAATGGTAATGAATT
GGTGAGGCCTCTTCAAAATGAATTGCGATTAATAATGTGGAAATATTGTGGGGTGATTAAAAACGAGACATTACTTTTAA
AGGGGTTATCAAAAATAGAAAGTATCAAAACAAAACTTAGTGATGTTGATGTAAGAATTGATCAGTATAATTGCGAAGAT
CTAGCATTGATCTTTGATTTACAATCTTCTTTGTTTAGTGCAAAAGCAACAATTATTTCGGCATTACAAAGAAATGAGAG
TAGGGGCGCACATCAAAGAAGTGATTTTCCCTTACTTGACCCCTTATTTAAATTTAATTGTGTAGTAAGCATGGATGATG
ATAATAATTTGAAAATCTCTAAAGTACCTCTAAAAGAATTAAATAAGGAACAAAAAATAATTGTTAAAAATGCAAAAAGA
GAAGAGGATATTAGAAATAAACTGCTTGAGTAA

Upstream 100 bases:

>100_bases
ATTATTTATGTCTTTCGAAAGCAAAGAGTTAAAATTAAATTACTAAATTAACTTAAAATTGCCTGTTAAAGAGCACCTCA
CTCAGATATCAAATGTTCTC

Downstream 100 bases:

>100_bases
TAGGGATATTTAATCCTTAATTAAATAGTTAATTACGTTCTTTATAAAAAAAATTAGATTAATGTAAAAATAAATTCTTG
ATCACTATCTGACCAAGTTT

Product: succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 570; Mature: 570

Protein sequence:

>570_residues
MIGCGGAGLRSAIEIKKSGLDVCILGKRPKTDAHTVLAAGGINAALGNLDKEDSWEQHFIDTYLEGYGIGDPLKVEIMAK
ESPSLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCF
GAMSFNLSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHPSGMVLPEEIEGTLV
TEAVRGEGGKLINNKGERFMREYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDISHKSKEFIIEKLPSIYRQFLE
AQMLDISKSPMEVAPTAHYSMGGILVNPEDLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNL
AQQMRSNKAIAIAHQNINKFIKNGNELVRPLQNELRLIMWKYCGVIKNETLLLKGLSKIESIKTKLSDVDVRIDQYNCED
LALIFDLQSSLFSAKATIISALQRNESRGAHQRSDFPLLDPLFKFNCVVSMDDDNNLKISKVPLKELNKEQKIIVKNAKR
EEDIRNKLLE

Sequences:

>Translated_570_residues
MIGCGGAGLRSAIEIKKSGLDVCILGKRPKTDAHTVLAAGGINAALGNLDKEDSWEQHFIDTYLEGYGIGDPLKVEIMAK
ESPSLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCF
GAMSFNLSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHPSGMVLPEEIEGTLV
TEAVRGEGGKLINNKGERFMREYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDISHKSKEFIIEKLPSIYRQFLE
AQMLDISKSPMEVAPTAHYSMGGILVNPEDLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNL
AQQMRSNKAIAIAHQNINKFIKNGNELVRPLQNELRLIMWKYCGVIKNETLLLKGLSKIESIKTKLSDVDVRIDQYNCED
LALIFDLQSSLFSAKATIISALQRNESRGAHQRSDFPLLDPLFKFNCVVSMDDDNNLKISKVPLKELNKEQKIIVKNAKR
EEDIRNKLLE
>Mature_570_residues
MIGCGGAGLRSAIEIKKSGLDVCILGKRPKTDAHTVLAAGGINAALGNLDKEDSWEQHFIDTYLEGYGIGDPLKVEIMAK
ESPSLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYSGDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCF
GAMSFNLSTSERTVHLADAVILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHPSGMVLPEEIEGTLV
TEAVRGEGGKLINNKGERFMREYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDISHKSKEFIIEKLPSIYRQFLE
AQMLDISKSPMEVAPTAHYSMGGILVNPEDLSTSVEGLFAAGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNL
AQQMRSNKAIAIAHQNINKFIKNGNELVRPLQNELRLIMWKYCGVIKNETLLLKGLSKIESIKTKLSDVDVRIDQYNCED
LALIFDLQSSLFSAKATIISALQRNESRGAHQRSDFPLLDPLFKFNCVVSMDDDNNLKISKVPLKELNKEQKIIVKNAKR
EEDIRNKLLE

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=533, Percent_Identity=33.3958724202627, Blast_Score=255, Evalue=7e-68,
Organism=Escherichia coli, GI1786942, Length=537, Percent_Identity=34.8230912476723, Blast_Score=254, Evalue=9e-69,
Organism=Escherichia coli, GI1790597, Length=519, Percent_Identity=33.5260115606936, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788928, Length=536, Percent_Identity=31.5298507462687, Blast_Score=233, Evalue=2e-62,
Organism=Caenorhabditis elegans, GI17505833, Length=528, Percent_Identity=33.1439393939394, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI17550100, Length=542, Percent_Identity=34.5018450184502, Blast_Score=271, Evalue=5e-73,
Organism=Caenorhabditis elegans, GI71986328, Length=479, Percent_Identity=27.348643006263, Blast_Score=105, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6322701, Length=534, Percent_Identity=34.4569288389513, Blast_Score=267, Evalue=4e-72,
Organism=Saccharomyces cerevisiae, GI6322416, Length=537, Percent_Identity=34.0782122905028, Blast_Score=254, Evalue=2e-68,
Organism=Saccharomyces cerevisiae, GI6322511, Length=469, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6320788, Length=471, Percent_Identity=28.2377919320594, Blast_Score=110, Evalue=5e-25,
Organism=Drosophila melanogaster, GI17137288, Length=534, Percent_Identity=33.1460674157303, Blast_Score=275, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24655642, Length=534, Percent_Identity=33.1460674157303, Blast_Score=275, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24655647, Length=534, Percent_Identity=33.1460674157303, Blast_Score=275, Evalue=9e-74,
Organism=Drosophila melanogaster, GI24663005, Length=531, Percent_Identity=33.7099811676083, Blast_Score=259, Evalue=2e-69,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 63393; Mature: 63393

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIGCGGAGLRSAIEIKKSGLDVCILGKRPKTDAHTVLAAGGINAALGNLDKEDSWEQHFI
CCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHCCCCCCCCHHHHHH
DTYLEGYGIGDPLKVEIMAKESPSLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYS
HHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCC
GDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCFGAMSFNLSTSERTVHLADAV
CCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEE
ILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHPSGMVLPEEIEGTLV
EEEECCCCEEECCCCCCCCCCCCCCEEEEECCCCEEEEEHEEEECCCCCCCHHHCCCEEE
TEAVRGEGGKLINNKGERFMREYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDIS
EHHHHCCCCCEECCHHHHHHHHCCCCCEEECCCCCEEECCHHHHHCCCCCCCCCEEEEEC
HKSKEFIIEKLPSIYRQFLEAQMLDISKSPMEVAPTAHYSMGGILVNPEDLSTSVEGLFA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECHHHHCHHHHHHHH
AGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNLAQQMRSNKAIAIAHQNINKF
HHHHHCCCCCCCCCCCCHHEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
IKNGNELVRPLQNELRLIMWKYCGVIKNETLLLKGLSKIESIKTKLSDVDVRIDQYNCED
HHCCHHHHHHHHHHHHHHHHHHHCHHCCCCCEEHHHHHHHHHHHHHHHCEEEEECCCCCC
LALIFDLQSSLFSAKATIISALQRNESRGAHQRSDFPLLDPLFKFNCVVSMDDDNNLKIS
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCEEEE
KVPLKELNKEQKIIVKNAKREEDIRNKLLE
ECCHHHCCCCCEEEEECCCHHHHHHHHHCC
>Mature Secondary Structure
MIGCGGAGLRSAIEIKKSGLDVCILGKRPKTDAHTVLAAGGINAALGNLDKEDSWEQHFI
CCCCCCCCHHHHHHHHHCCCCEEEECCCCCCCCCEEEEECCCHHHHCCCCCCCCHHHHHH
DTYLEGYGIGDPLKVEIMAKESPSLVKEIDKWGANFAKLKNGELDQRFFGAHKYRRTCYS
HHHHHCCCCCCCEEEEEEECCCCHHHHHHHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCC
GDFTGLSILKTLLKKSDDLKIPIYDNQYVTELLIRENTCFGAMSFNLSTSERTVHLADAV
CCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEEE
ILCTGGHTRLWKKSSSRKNENTGDGYYLSLKGGCELIDMEMVQFHPSGMVLPEEIEGTLV
EEEECCCCEEECCCCCCCCCCCCCCEEEEECCCCEEEEEHEEEECCCCCCCHHHCCCEEE
TEAVRGEGGKLINNKGERFMREYDPKRMELSTRDRVAIANYTEIIEGRGTKNGGVFLDIS
EHHHHCCCCCEECCHHHHHHHHCCCCCEEECCCCCEEECCHHHHHCCCCCCCCCEEEEEC
HKSKEFIIEKLPSIYRQFLEAQMLDISKSPMEVAPTAHYSMGGILVNPEDLSTSVEGLFA
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEEECHHHHCHHHHHHHH
AGEVAGGLHGANRLGGNSLAEIIIFGRRAGIAASKYSKNLAQQMRSNKAIAIAHQNINKF
HHHHHCCCCCCCCCCCCHHEEHEEEECCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHH
IKNGNELVRPLQNELRLIMWKYCGVIKNETLLLKGLSKIESIKTKLSDVDVRIDQYNCED
HHCCHHHHHHHHHHHHHHHHHHHCHHCCCCCEEHHHHHHHHHHHHHHHCEEEEECCCCCC
LALIFDLQSSLFSAKATIISALQRNESRGAHQRSDFPLLDPLFKFNCVVSMDDDNNLKIS
EEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCEEEEEECCCCCEEEE
KVPLKELNKEQKIIVKNAKREEDIRNKLLE
ECCHHHCCCCCEEEEECCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.02 {succinate}} 0.3 {ferricyanide}} 0.4 {fumarate}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]