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The map label for this gene is phoB [H]

Identifier: 126696581

GI number: 126696581

Start: 1055841

End: 1056569

Strand: Reverse

Name: phoB [H]

Synonym: P9301_12431

Alternate gene names: 126696581

Gene position: 1056569-1055841 (Counterclockwise)

Preceding gene: 126696582

Following gene: 126696580

Centisome position: 64.35

GC content: 30.32

Gene sequence:

>729_bases
ATGGCTATTAAAGATCATAAAAGTTTGCAAGACTCAAGAATTCTTCTTGTAGAGGATGATAAGAGTATTAGGCTTACAGT
CAGTGAAACCTTGAGCAGCGAAGGTTTTAGAGTATCAAGTTTCAAAGACGGTTTAAGTGCCTTAGATTTTATTAATAATG
ATATTAAAAAAGATGTTGACCTAATAATTCTAGATTTAATGTTGCCAGGACTAAATGGATTAGAGTTATGTAGAAAAATA
AGAAATGATGAAGACTATACACCCATACTAATTTTGAGTGCTAAAGATAATGAATCAGACAGGGTTCTTGGATTAGAGGT
TGGTGCAGATGATTATTTAACAAAACCTTTTGGTTTAAATGAATTAATTGCTAGATCTAGAGCCTTAATAAGAAGATCTA
AACGTAATAAAAAAAATATAGAAAAATCACACACTGTAATCGAGTTTAATCACATAAAAATGTTTCTGGAAGAATGTAGG
GTAACTTCTTTTGATAAAGAAATAACATTATCTCCAAAAGAATTTAAATTATTAGAGTTATTTATGAAAAATCCAAAAAG
GGTATGGTCAAGAGATTTAATTCTGGAAAAAATTTGGGAAATTGACTTTATTGGTGATACTAAAACTGTAGATGTTCATG
TTAGGTGGCTCAGAGAGAAACTAGAAGAAGATCCCTCAGCTCCAAAGTTTCTTAAAACTGTAAGAGGTTTTGGATATAAG
TTTGGATGA

Upstream 100 bases:

>100_bases
TAAGATTAAATGAAAAAAATAATAATTCAATGTAAATTAATTATGAAATAATTAGATCAAATAATTTTGATTATATAAAA
TAAAATTGTTGGAACTTTCA

Downstream 100 bases:

>100_bases
AAATGAAAACTCTACAAGAAGTATTATTGTTTTTACATCAGAAGTGGAAAATAAAAGTCAAGCATTTTTCTCAATCAAAA
GAAAAAAAATTTGAAGTTGA

Product: two-component response regulator, phosphate

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 241

Protein sequence:

>242_residues
MAIKDHKSLQDSRILLVEDDKSIRLTVSETLSSEGFRVSSFKDGLSALDFINNDIKKDVDLIILDLMLPGLNGLELCRKI
RNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLNELIARSRALIRRSKRNKKNIEKSHTVIEFNHIKMFLEECR
VTSFDKEITLSPKEFKLLELFMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
FG

Sequences:

>Translated_242_residues
MAIKDHKSLQDSRILLVEDDKSIRLTVSETLSSEGFRVSSFKDGLSALDFINNDIKKDVDLIILDLMLPGLNGLELCRKI
RNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLNELIARSRALIRRSKRNKKNIEKSHTVIEFNHIKMFLEECR
VTSFDKEITLSPKEFKLLELFMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
FG
>Mature_241_residues
AIKDHKSLQDSRILLVEDDKSIRLTVSETLSSEGFRVSSFKDGLSALDFINNDIKKDVDLIILDLMLPGLNGLELCRKIR
NDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLNELIARSRALIRRSKRNKKNIEKSHTVIEFNHIKMFLEECRV
TSFDKEITLSPKEFKLLELFMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYKF
G

Specific function: Member of the two-component regulatory system sphR/sphS. Response regulator. Involved in inducible production of alkaline phosphatase in response to phosphate limitation as it is directly involved in the regulation of phoA transcription in response to pho

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1786599, Length=232, Percent_Identity=39.6551724137931, Blast_Score=157, Evalue=5e-40,
Organism=Escherichia coli, GI1790860, Length=233, Percent_Identity=37.3390557939914, Blast_Score=155, Evalue=2e-39,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=33.9055793991416, Blast_Score=153, Evalue=9e-39,
Organism=Escherichia coli, GI87082012, Length=229, Percent_Identity=37.9912663755458, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=40.0881057268723, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1789402, Length=232, Percent_Identity=41.3793103448276, Blast_Score=150, Evalue=7e-38,
Organism=Escherichia coli, GI1786911, Length=230, Percent_Identity=33.0434782608696, Blast_Score=136, Evalue=1e-33,
Organism=Escherichia coli, GI1790863, Length=236, Percent_Identity=33.8983050847458, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=33.6206896551724, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1790552, Length=227, Percent_Identity=33.920704845815, Blast_Score=117, Evalue=6e-28,
Organism=Escherichia coli, GI2367329, Length=232, Percent_Identity=34.9137931034483, Blast_Score=114, Evalue=4e-27,
Organism=Escherichia coli, GI1787375, Length=229, Percent_Identity=31.8777292576419, Blast_Score=112, Evalue=2e-26,
Organism=Escherichia coli, GI1787229, Length=230, Percent_Identity=31.7391304347826, Blast_Score=101, Evalue=5e-23,
Organism=Escherichia coli, GI145693140, Length=235, Percent_Identity=28.0851063829787, Blast_Score=95, Evalue=5e-21,
Organism=Escherichia coli, GI1790299, Length=116, Percent_Identity=30.1724137931034, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1788550, Length=146, Percent_Identity=31.5068493150685, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787487, Length=110, Percent_Identity=36.3636363636364, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 28059; Mature: 27928

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKDHKSLQDSRILLVEDDKSIRLTVSETLSSEGFRVSSFKDGLSALDFINNDIKKDVD
CCCCCCCCCCCCEEEEEECCCCEEEEEEHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHH
LIILDLMLPGLNGLELCRKIRNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLN
HHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHCCCCCCHH
ELIARSRALIRRSKRNKKNIEKSHTVIEFNHIKMFLEECRVTSFDKEITLSPKEFKLLEL
HHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHH
FMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
HHHCHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
FG
CC
>Mature Secondary Structure 
AIKDHKSLQDSRILLVEDDKSIRLTVSETLSSEGFRVSSFKDGLSALDFINNDIKKDVD
CCCCCCCCCCCEEEEEECCCCEEEEEEHHHCCCCEEECHHHHHHHHHHHHHHHHHHHHH
LIILDLMLPGLNGLELCRKIRNDEDYTPILILSAKDNESDRVLGLEVGADDYLTKPFGLN
HHHHHHHHCCCCHHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEECCCHHCCCCCCHH
ELIARSRALIRRSKRNKKNIEKSHTVIEFNHIKMFLEECRVTSFDKEITLSPKEFKLLEL
HHHHHHHHHHHHHHHHHHHHHHHCCEEEHHHHHHHHHHHCCCCCCCEEECCCHHHHHHHH
FMKNPKRVWSRDLILEKIWEIDFIGDTKTVDVHVRWLREKLEEDPSAPKFLKTVRGFGYK
HHHCHHHHHHHHHHHHHHHHEEECCCCEEEEHHHHHHHHHHHCCCCCHHHHHHHHHCCCC
FG
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8497200; 8157591 [H]