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The map label for this gene is gid

Identifier: 126696561

GI number: 126696561

Start: 1038600

End: 1040012

Strand: Reverse

Name: gid

Synonym: P9301_12231

Alternate gene names: 126696561

Gene position: 1040012-1038600 (Counterclockwise)

Preceding gene: 126696562

Following gene: 126696560

Centisome position: 63.34

GC content: 33.9

Gene sequence:

>1413_bases
TTGTTAGATAAAGAAGTATTAGTTATTGGAGCTGGTCTTGCAGGATCTGAAGCTGCTTGGCAATTAGCAAATTCTGGTGT
ACCAGTTAAGTTAGTTGAAATGAGACCTATTAAATCAACTCCAGCTCATCATACGAGTGAATTTGGAGAATTGGTTTGTA
GTAATAGTTTTGGAGCTTTAAGTCCTGATAGAGCTGCAGGTTTATTGCAAAAAGAACTTAGGTTTTTTAAATCTTTGATT
GTTCAAACAGCTGATAAATTTGCTGTTCCAGCCGGAGGTGCTTTGGCGGTTGATAGATCTAAATTTAGTTACGCTTTGAC
TGAACAATTATCAAATCATCCTTTAGTCGAAATTAAGAGATTTGAACAACTAGATATCCCAAGTGAAGAAAATATAACTA
TCCTTGCAACTGGGCCATTAACTGCCGATGATTTATCCTATAAAATCCAAGATTTTACTGGTATCGATGATTGTCATTTT
TTTGACGCGGCTAGCCCCATAATTTATGGAGATTCTATTGATCAAGAGATTGTATTTAAAGCTAGTAGATACGACAAAGG
AGATCCTGCATATCTAAATTGCCCTATGGATGAAAATAAATACATCCATTTCAGGAATGAACTTATAGAAGGAGAACAAG
CTAATTTAAAAGACTTTGAGAAAGAATCAGCTAATTTCTTTGAGGCTTGCTTACCAATTGAAGAAATTGCTAGAAGAGGA
GTTGATACAATGAGATATGGGCCATTGAAATCTATTGGTTTGTGGAATCCAAAATGGGGAGATTTATTTGATAGGGAAAA
TAGGTTAAAAAAGCGACCTCATGCAGTTGTCCAATTAAGGAAAGAAGATTTAGAAGGGAAATTACTAAATATGGTAGGTT
TTCAAACTAACCTCAAATGGTCTGAGCAAAAAAGAATATTTAGGATGATTCCTGGTTTAGAAAAGGCCGAGTTTGTACGT
TTTGGAGTAATGCATAGAAATACTTTTTTAGAATCTCCAAAATTACTTTTACCAACATTGCAATTTATGAAAAGAGAAAA
CCTTTTTGCAGCGGGTCAAATAACTGGGACGGAAGGTTATGCAGCAGCAGCTGCAGGAGGCTTGCTTGCAGGAATAAATG
CATCCTTATTAGCTAAGGGTAAAAAACCAGTAAGTTTTCCTGATGAATCAATGATTGGTTCTCTAATGAATTTTATTAGT
AACAAAAATCAAATATTATCTAATCAGAAAAAGAATAAATTCCAACCAATGCCTGCTTCATTTGGTTTAGTTCCAGAACT
AACTAAAAGAATAAAAGATAAAAGATTAAGGTACAAAGCTTATCAAGAAAGATCTACAGAAGCCTTGAATGACTTTAAAA
ATAAACTAGATTCTTGTTTTGATAAAGACCACTTACTCAGCAAAATTTACTAA

Upstream 100 bases:

>100_bases
TGCACCCATTATCGCTGCTTTAGCTTGGGTTATATTCAATATACAAAAACCAGCAAGAGAGCAATTCAATAGGGACTTTT
TGGGCAAGGATTAATCTAAT

Downstream 100 bases:

>100_bases
GATAAATTATCAAGGATCAAAAAATGGAATTAAATAAGGAAAACTTCGATGCAATTATTATTGGCTCAGGAATAGGAGGG
TTAGTAACTGCTTCACAATT

Product: tRNA (uracil-5-)-methyltransferase Gid

Products: NA

Alternate protein names: Folate-dependent tRNA (uracil-5-)-methyltransferase; Folate-dependent tRNA(M-5-U54)-methyltransferase

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MLDKEVLVIGAGLAGSEAAWQLANSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGALSPDRAAGLLQKELRFFKSLI
VQTADKFAVPAGGALAVDRSKFSYALTEQLSNHPLVEIKRFEQLDIPSEENITILATGPLTADDLSYKIQDFTGIDDCHF
FDAASPIIYGDSIDQEIVFKASRYDKGDPAYLNCPMDENKYIHFRNELIEGEQANLKDFEKESANFFEACLPIEEIARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRMIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFMKRENLFAAGQITGTEGYAAAAAGGLLAGINASLLAKGKKPVSFPDESMIGSLMNFIS
NKNQILSNQKKNKFQPMPASFGLVPELTKRIKDKRLRYKAYQERSTEALNDFKNKLDSCFDKDHLLSKIY

Sequences:

>Translated_470_residues
MLDKEVLVIGAGLAGSEAAWQLANSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGALSPDRAAGLLQKELRFFKSLI
VQTADKFAVPAGGALAVDRSKFSYALTEQLSNHPLVEIKRFEQLDIPSEENITILATGPLTADDLSYKIQDFTGIDDCHF
FDAASPIIYGDSIDQEIVFKASRYDKGDPAYLNCPMDENKYIHFRNELIEGEQANLKDFEKESANFFEACLPIEEIARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRMIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFMKRENLFAAGQITGTEGYAAAAAGGLLAGINASLLAKGKKPVSFPDESMIGSLMNFIS
NKNQILSNQKKNKFQPMPASFGLVPELTKRIKDKRLRYKAYQERSTEALNDFKNKLDSCFDKDHLLSKIY
>Mature_470_residues
MLDKEVLVIGAGLAGSEAAWQLANSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGALSPDRAAGLLQKELRFFKSLI
VQTADKFAVPAGGALAVDRSKFSYALTEQLSNHPLVEIKRFEQLDIPSEENITILATGPLTADDLSYKIQDFTGIDDCHF
FDAASPIIYGDSIDQEIVFKASRYDKGDPAYLNCPMDENKYIHFRNELIEGEQANLKDFEKESANFFEACLPIEEIARRG
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKWSEQKRIFRMIPGLEKAEFVR
FGVMHRNTFLESPKLLLPTLQFMKRENLFAAGQITGTEGYAAAAAGGLLAGINASLLAKGKKPVSFPDESMIGSLMNFIS
NKNQILSNQKKNKFQPMPASFGLVPELTKRIKDKRLRYKAYQERSTEALNDFKNKLDSCFDKDHLLSKIY

Specific function: Catalyzes the folate-dependent formation of 5-methyl- uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG1206

COG function: function code J; NAD(FAD)-utilizing enzyme possibly involved in translation

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family. TrmFO subfamily

Homologues:

Organism=Homo sapiens, GI74024895, Length=135, Percent_Identity=36.2962962962963, Blast_Score=71, Evalue=2e-12,
Organism=Escherichia coli, GI2367273, Length=412, Percent_Identity=24.5145631067961, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17534255, Length=433, Percent_Identity=24.9422632794457, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6321202, Length=519, Percent_Identity=24.0847784200385, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMFO_PROM0 (A3PDM1)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091447.1
- ProteinModelPortal:   A3PDM1
- SMR:   A3PDM1
- STRING:   A3PDM1
- GeneID:   4911344
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_12231
- eggNOG:   COG1206
- HOGENOM:   HBG285041
- OMA:   FEGCLPI
- ProtClustDB:   PRK05335
- BioCyc:   PMAR167546:P9301ORF_1244-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01037
- InterPro:   IPR004417
- InterPro:   IPR002218
- InterPro:   IPR020595
- TIGRFAMs:   TIGR00137

Pfam domain/function: PF01134 GIDA

EC number: =2.1.1.74

Molecular weight: Translated: 52800; Mature: 52800

Theoretical pI: Translated: 8.19; Mature: 8.19

Prosite motif: PS01280 GIDA_1; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKEVLVIGAGLAGSEAAWQLANSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGAL
CCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
SPDRAAGLLQKELRFFKSLIVQTADKFAVPAGGALAVDRSKFSYALTEQLSNHPLVEIKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEH
FEQLDIPSEENITILATGPLTADDLSYKIQDFTGIDDCHFFDAASPIIYGDSIDQEIVFK
HHHCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCHHHCCCCCEEECCCCCHHHHEE
ASRYDKGDPAYLNCPMDENKYIHFRNELIEGEQANLKDFEKESANFFEACLPIEEIARRG
HHCCCCCCCCEEECCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCC
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCCCH
SEQKRIFRMIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFMKRENLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECEEECCCCC
AAAAAGGLLAGINASLLAKGKKPVSFPDESMIGSLMNFISNKNQILSNQKKNKFQPMPAS
HHHHHCCHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCC
FGLVPELTKRIKDKRLRYKAYQERSTEALNDFKNKLDSCFDKDHLLSKIY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC
>Mature Secondary Structure
MLDKEVLVIGAGLAGSEAAWQLANSGVPVKLVEMRPIKSTPAHHTSEFGELVCSNSFGAL
CCCCEEEEEECCCCCCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCC
SPDRAAGLLQKELRFFKSLIVQTADKFAVPAGGALAVDRSKFSYALTEQLSNHPLVEIKR
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEH
FEQLDIPSEENITILATGPLTADDLSYKIQDFTGIDDCHFFDAASPIIYGDSIDQEIVFK
HHHCCCCCCCCEEEEECCCCCCCCCCEEECCCCCCCCCHHHCCCCCEEECCCCCHHHHEE
ASRYDKGDPAYLNCPMDENKYIHFRNELIEGEQANLKDFEKESANFFEACLPIEEIARRG
HHCCCCCCCCEEECCCCCCCEEEEHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHCC
VDTMRYGPLKSIGLWNPKWGDLFDRENRLKKRPHAVVQLRKEDLEGKLLNMVGFQTNLKW
CCHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHCCCCCCCH
SEQKRIFRMIPGLEKAEFVRFGVMHRNTFLESPKLLLPTLQFMKRENLFAAGQITGTEGY
HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEECEEECCCCC
AAAAAGGLLAGINASLLAKGKKPVSFPDESMIGSLMNFISNKNQILSNQKKNKFQPMPAS
HHHHHCCHHHCCCHHHHCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCCC
FGLVPELTKRIKDKRLRYKAYQERSTEALNDFKNKLDSCFDKDHLLSKIY
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA