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The map label for this gene is lasT [C]

Identifier: 126696500

GI number: 126696500

Start: 974375

End: 975127

Strand: Reverse

Name: lasT [C]

Synonym: P9301_11621

Alternate gene names: 126696500

Gene position: 975127-974375 (Counterclockwise)

Preceding gene: 126696501

Following gene: 126696499

Centisome position: 59.39

GC content: 28.15

Gene sequence:

>753_bases
ATGATTTTGGGAAAAAATTTTTCTAATTTAAAGGTAATTTTAGTTGAACCAAATGGCCCCTTAAATGTAGGAAGCGTTGC
TAGATTATGCAGTAATTTTGAAGTTGATGAATTAAGAATTGTTTCTCCAAAATGCGATATATTTTCTTTAGAAGCAAGAA
AAATGGCTCTTAAAGGTCAAAAATTTCTTAAACATTGTAGGGTTTTTGATGATCTTCAAAAAGCAGTTTTTGATTGTGAT
TTGGTTCTAGCCTCTTGTGGGAGGATTGATGTAAATAAAGATTCTTTTTTTGTATCTTCTGAAGATATATTTGATTGGAC
TTTATCCTTTAAGAAGATAAATAATTTAGCAATTATATTTGGAAGAGAAGATAGAGGTTTAACTAACAATGAATTGCTTC
TAGCAAATAAAACTTTTAATATTCCAACTTCTCAGGATAATCCTTCATTAAATCTTTCCCACGCTGTTTCAATAGTTTTA
TATGAATTAAATAAGTCTTCTAAAAAGAATTTAAATAATGAATCAAGAGCTTTTAACTTAGCATCATCGAAAGCAGTGCA
TGATACATTTATGGAGATAGAGGAAATGCTTATGCGAGTTGGATATCTTTTAAAACATACCTCTAAGGCAAAAATCAGTA
AATTTAAGAATTTTACTTTAAGGGCAAATACATCAATGCACGAAATAAATGTTTTAAGAGGAATTGTCCATCAAATAAAC
TGGTTTTTGAACAATTCAAAAAAAAATAAGTAA

Upstream 100 bases:

>100_bases
TAATAAAACAAGTTACTGGAGGCCTGACTCCTCCAATGCCAAGTTTTGAAATTGATCCTGTAAATATGTCCAATTTATTA
AAATATCTTCATAGTCTTGA

Downstream 100 bases:

>100_bases
GTATAAATTTGATTAGTTGTTAATTACTTCTTGTTTAAATTTGATAGGGATATTTACTAAAAACATTTTCTGAAGTAGCA
TCTATTGATTATTTGAATAT

Product: tRNA/rRNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 250

Protein sequence:

>250_residues
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEARKMALKGQKFLKHCRVFDDLQKAVFDCD
LVLASCGRIDVNKDSFFVSSEDIFDWTLSFKKINNLAIIFGREDRGLTNNELLLANKTFNIPTSQDNPSLNLSHAVSIVL
YELNKSSKKNLNNESRAFNLASSKAVHDTFMEIEEMLMRVGYLLKHTSKAKISKFKNFTLRANTSMHEINVLRGIVHQIN
WFLNNSKKNK

Sequences:

>Translated_250_residues
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEARKMALKGQKFLKHCRVFDDLQKAVFDCD
LVLASCGRIDVNKDSFFVSSEDIFDWTLSFKKINNLAIIFGREDRGLTNNELLLANKTFNIPTSQDNPSLNLSHAVSIVL
YELNKSSKKNLNNESRAFNLASSKAVHDTFMEIEEMLMRVGYLLKHTSKAKISKFKNFTLRANTSMHEINVLRGIVHQIN
WFLNNSKKNK
>Mature_250_residues
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEARKMALKGQKFLKHCRVFDDLQKAVFDCD
LVLASCGRIDVNKDSFFVSSEDIFDWTLSFKKINNLAIIFGREDRGLTNNELLLANKTFNIPTSQDNPSLNLSHAVSIVL
YELNKSSKKNLNNESRAFNLASSKAVHDTFMEIEEMLMRVGYLLKHTSKAKISKFKNFTLRANTSMHEINVLRGIVHQIN
WFLNNSKKNK

Specific function: Unknown

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1790865, Length=206, Percent_Identity=27.6699029126214, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1788881, Length=239, Percent_Identity=24.6861924686192, Blast_Score=79, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28418; Mature: 28418

Theoretical pI: Translated: 9.87; Mature: 9.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEARKMALKGQ
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEHHHHHHHHHHH
KFLKHCRVFDDLQKAVFDCDLVLASCGRIDVNKDSFFVSSEDIFDWTLSFKKINNLAIIF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCEEEEEEEEECCCEEEEE
GREDRGLTNNELLLANKTFNIPTSQDNPSLNLSHAVSIVLYELNKSSKKNLNNESRAFNL
ECCCCCCCCCEEEEEECEECCCCCCCCCCCCHHHHEEEEEEECCCCHHHCCCCCHHEEEC
ASSKAVHDTFMEIEEMLMRVGYLLKHTSKAKISKFKNFTLRANTSMHEINVLRGIVHQIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
WFLNNSKKNK
HHHCCCCCCC
>Mature Secondary Structure
MILGKNFSNLKVILVEPNGPLNVGSVARLCSNFEVDELRIVSPKCDIFSLEARKMALKGQ
CCCCCCCCCEEEEEECCCCCCCHHHHHHHHCCCCCCCEEEECCCCCEEEHHHHHHHHHHH
KFLKHCRVFDDLQKAVFDCDLVLASCGRIDVNKDSFFVSSEDIFDWTLSFKKINNLAIIF
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEECCCCEEEEEEEEECCCEEEEE
GREDRGLTNNELLLANKTFNIPTSQDNPSLNLSHAVSIVLYELNKSSKKNLNNESRAFNL
ECCCCCCCCCEEEEEECEECCCCCCCCCCCCHHHHEEEEEEECCCCHHHCCCCCHHEEEC
ASSKAVHDTFMEIEEMLMRVGYLLKHTSKAKISKFKNFTLRANTSMHEINVLRGIVHQIN
HHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCEEEEECCCHHHHHHHHHHHHHHH
WFLNNSKKNK
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]