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The map label for this gene is ruvC

Identifier: 126696498

GI number: 126696498

Start: 972690

End: 973163

Strand: Reverse

Name: ruvC

Synonym: P9301_11601

Alternate gene names: 126696498

Gene position: 973163-972690 (Counterclockwise)

Preceding gene: 126696499

Following gene: 126696497

Centisome position: 59.27

GC content: 34.81

Gene sequence:

>474_bases
GTGAGAATAATTGGGATTGACCCTGGATTAGCTAGAGTTGGTTATGGAATAATTGAGATAGAAAATGAAAGAAAGATATT
ATTGGATTGCGGCGTTATTGAGACAGGTAAAGATAAAAAAGAAGAAGATAGACTTTATGAGATATTCCAAGATCTTAATG
AATTAATAAATCATTGGAATCCAACTTCAGCTGCAGTAGAAAAATTTTTCTTTTACAGGTCAAGTACCACAATTAGTGTC
GTGCAGGCCAGAGGCGTGATTATGATGGTATTGGCCTCTAAAAAAATTAATGTTAGTGAGTATTCACCTGCTCAGATAAA
ATTAACAATTGCTGGGTCTGGAAAGGCATCTAAGAAAGATATTCTTGATGCTGTTATGTATAACTTAGATCTAAACAAAC
CTCCAAAACCTGATGATTCAGCAGATGCATTGGCAATAGCACTCACAAAACTAAATGAGGATGGCTTTAACTGA

Upstream 100 bases:

>100_bases
ATTCAGGTGAAAGAGTTATTCAAGCTTTTTGTAAAGTATTTGATTTAGATGAGAAAGAAAATCTTTCAAAATTTCAATTG
TCTGCTGAAGCTTAATAAAA

Downstream 100 bases:

>100_bases
AAATTTAATATGACGATCTTTCAAAATAAGAAATTGGAAAGGGATACGTTTAGAAAATTAAGAGATGAAATTTCTTTTAA
TCAAAGAGAAAATGTTGAAA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC

Number of amino acids: Translated: 157; Mature: 157

Protein sequence:

>157_residues
MRIIGIDPGLARVGYGIIEIENERKILLDCGVIETGKDKKEEDRLYEIFQDLNELINHWNPTSAAVEKFFFYRSSTTISV
VQARGVIMMVLASKKINVSEYSPAQIKLTIAGSGKASKKDILDAVMYNLDLNKPPKPDDSADALAIALTKLNEDGFN

Sequences:

>Translated_157_residues
MRIIGIDPGLARVGYGIIEIENERKILLDCGVIETGKDKKEEDRLYEIFQDLNELINHWNPTSAAVEKFFFYRSSTTISV
VQARGVIMMVLASKKINVSEYSPAQIKLTIAGSGKASKKDILDAVMYNLDLNKPPKPDDSADALAIALTKLNEDGFN
>Mature_157_residues
MRIIGIDPGLARVGYGIIEIENERKILLDCGVIETGKDKKEEDRLYEIFQDLNELINHWNPTSAAVEKFFFYRSSTTISV
VQARGVIMMVLASKKINVSEYSPAQIKLTIAGSGKASKKDILDAVMYNLDLNKPPKPDDSADALAIALTKLNEDGFN

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family

Homologues:

Organism=Escherichia coli, GI1788170, Length=135, Percent_Identity=31.8518518518519, Blast_Score=69, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVC_PROM0 (A3PDF8)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091384.1
- ProteinModelPortal:   A3PDF8
- SMR:   A3PDF8
- STRING:   A3PDF8
- GeneID:   4912043
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_11601
- eggNOG:   COG0817
- HOGENOM:   HBG437552
- OMA:   RTGWGVI
- ProtClustDB:   PRK00039
- BioCyc:   PMAR167546:P9301ORF_1181-MONOMER
- HAMAP:   MF_00034
- InterPro:   IPR012337
- InterPro:   IPR002176
- PRINTS:   PR00696

Pfam domain/function: PF02075 RuvC; SSF53098 RNaseH_fold

EC number: =3.1.22.4

Molecular weight: Translated: 17420; Mature: 17420

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIIGIDPGLARVGYGIIEIENERKILLDCGVIETGKDKKEEDRLYEIFQDLNELINHWN
CEEEECCCCHHHHCCEEEEECCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
PTSAAVEKFFFYRSSTTISVVQARGVIMMVLASKKINVSEYSPAQIKLTIAGSGKASKKD
CHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHH
ILDAVMYNLDLNKPPKPDDSADALAIALTKLNEDGFN
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCC
>Mature Secondary Structure
MRIIGIDPGLARVGYGIIEIENERKILLDCGVIETGKDKKEEDRLYEIFQDLNELINHWN
CEEEECCCCHHHHCCEEEEECCCCEEEEECCCEECCCCCCHHHHHHHHHHHHHHHHHCCC
PTSAAVEKFFFYRSSTTISVVQARGVIMMVLASKKINVSEYSPAQIKLTIAGSGKASKKD
CHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHH
ILDAVMYNLDLNKPPKPDDSADALAIALTKLNEDGFN
HHHHHHHHCCCCCCCCCCCCCCCEEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA