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The map label for this gene is tpiA

Identifier: 126696379

GI number: 126696379

Start: 901889

End: 902614

Strand: Reverse

Name: tpiA

Synonym: P9301_10411

Alternate gene names: 126696379

Gene position: 902614-901889 (Counterclockwise)

Preceding gene: 126696380

Following gene: 126696378

Centisome position: 54.97

GC content: 34.99

Gene sequence:

>726_bases
TTGAGAAAATCTGTAATTGCTGGTAATTGGAAAATGCACATGACTTGTGCTGAAGCTAAATCCTATTTAGAAGAGTTTAT
TCCTTTAATAAAAAACATTAAAGACGATCGTAAAGTTGTTATTGCTCCACCTTTTACGGCTATTTCAACCTTTTCTGATC
ATTCTGATTTTGAATATTTAGATATTTCTAGTCAAAATATTCATTGGGAAGATCAAGGAGCGTTTACGGCAGAAATATCC
CCCAAAATGCTTCTTGAACATGGTGTCTCATATGCAATCGTTGGACATAGTGAACCAAGAAAATATTTTAGTGAAAGTGA
TGAGCAAATTAACAAAAGAGCAGTTTTTGCTCAATCAAGTGGACTTACTCCAATAGTTTGTGTAGGAGAAACATTAGAAC
AAAGAGAGAGAGGAGAGGCTGATAGAGTTATTACTAGACAGGTTGAACAAGGATTAGAAAATACAGATCCATCTAATTTA
ATTGTTGCCTATGAACCAATATGGGCTATTGGCACTGGTAAAACGTGTGAGGCAGAAGACGCTAATAAGATATGTTCTTT
GATTCGGAAATTAATAGGTTTTGATGATGTAATTATTCAATATGGAGGATCTGTTAAGCCTAATAATATCGACGAAATCA
TGTCAATGAGTGATATCGATGGGGTGCTAGTCGGAGGGGCTTCATTAGATCCGAATAGTTTCGCGAGAATTGCAAATTAT
CAATAA

Upstream 100 bases:

>100_bases
AAGGGGGATAAAGTAATATTTCTAAAAAATGAATTAATTTTTGAATAGTTAGCTTATTCAACTCACTTTGTATTAGTATA
TTTTTCTTGGAGATAGTATT

Downstream 100 bases:

>100_bases
GAAAAATAAATGGCCAAAAGGCTGGGGCCAAAAAACTTCAATTATGGGAGTTATAAATTTAACTCCTGATTCATTTAGTG
ATGGTGGGGAAATAAACTCT

Product: triosephosphate isomerase

Products: NA

Alternate protein names: TIM; Triose-phosphate isomerase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MRKSVIAGNWKMHMTCAEAKSYLEEFIPLIKNIKDDRKVVIAPPFTAISTFSDHSDFEYLDISSQNIHWEDQGAFTAEIS
PKMLLEHGVSYAIVGHSEPRKYFSESDEQINKRAVFAQSSGLTPIVCVGETLEQRERGEADRVITRQVEQGLENTDPSNL
IVAYEPIWAIGTGKTCEAEDANKICSLIRKLIGFDDVIIQYGGSVKPNNIDEIMSMSDIDGVLVGGASLDPNSFARIANY
Q

Sequences:

>Translated_241_residues
MRKSVIAGNWKMHMTCAEAKSYLEEFIPLIKNIKDDRKVVIAPPFTAISTFSDHSDFEYLDISSQNIHWEDQGAFTAEIS
PKMLLEHGVSYAIVGHSEPRKYFSESDEQINKRAVFAQSSGLTPIVCVGETLEQRERGEADRVITRQVEQGLENTDPSNL
IVAYEPIWAIGTGKTCEAEDANKICSLIRKLIGFDDVIIQYGGSVKPNNIDEIMSMSDIDGVLVGGASLDPNSFARIANY
Q
>Mature_241_residues
MRKSVIAGNWKMHMTCAEAKSYLEEFIPLIKNIKDDRKVVIAPPFTAISTFSDHSDFEYLDISSQNIHWEDQGAFTAEIS
PKMLLEHGVSYAIVGHSEPRKYFSESDEQINKRAVFAQSSGLTPIVCVGETLEQRERGEADRVITRQVEQGLENTDPSNL
IVAYEPIWAIGTGKTCEAEDANKICSLIRKLIGFDDVIIQYGGSVKPNNIDEIMSMSDIDGVLVGGASLDPNSFARIANY
Q

Specific function: Plays an important role in several metabolic pathways. [C]

COG id: COG0149

COG function: function code G; Triosephosphate isomerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the triosephosphate isomerase family

Homologues:

Organism=Homo sapiens, GI226529917, Length=248, Percent_Identity=39.1129032258064, Blast_Score=157, Evalue=1e-38,
Organism=Homo sapiens, GI4507645, Length=250, Percent_Identity=38.8, Blast_Score=156, Evalue=1e-38,
Organism=Escherichia coli, GI1790353, Length=252, Percent_Identity=37.6984126984127, Blast_Score=167, Evalue=7e-43,
Organism=Caenorhabditis elegans, GI17536593, Length=246, Percent_Identity=38.2113821138211, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6320255, Length=246, Percent_Identity=36.1788617886179, Blast_Score=144, Evalue=2e-35,
Organism=Drosophila melanogaster, GI28572008, Length=247, Percent_Identity=40.080971659919, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI28572006, Length=247, Percent_Identity=40.080971659919, Blast_Score=155, Evalue=2e-38,
Organism=Drosophila melanogaster, GI28572004, Length=239, Percent_Identity=40.1673640167364, Blast_Score=155, Evalue=3e-38,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TPIS_PROM0 (A3PD39)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001091265.1
- ProteinModelPortal:   A3PD39
- SMR:   A3PD39
- STRING:   A3PD39
- GeneID:   4912002
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_10411
- eggNOG:   COG0149
- HOGENOM:   HBG708281
- OMA:   DIRSVQT
- ProtClustDB:   PRK00042
- BioCyc:   PMAR167546:P9301ORF_1060-MONOMER
- GO:   GO:0005737
- GO:   GO:0006094
- GO:   GO:0006096
- HAMAP:   MF_00147_B
- InterPro:   IPR013785
- InterPro:   IPR022896
- InterPro:   IPR000652
- InterPro:   IPR020861
- Gene3D:   G3DSA:3.20.20.70
- PANTHER:   PTHR21139
- TIGRFAMs:   TIGR00419

Pfam domain/function: PF00121 TIM; SSF51351 Triophos_ismrse

EC number: =5.3.1.1

Molecular weight: Translated: 26779; Mature: 26779

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00171 TIM_1; PS51440 TIM_2

Important sites: ACT_SITE 96-96 ACT_SITE 165-165 BINDING 9-9 BINDING 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKSVIAGNWKMHMTCAEAKSYLEEFIPLIKNIKDDRKVVIAPPFTAISTFSDHSDFEYL
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCEEE
DISSQNIHWEDQGAFTAEISPKMLLEHGVSYAIVGHSEPRKYFSESDEQINKRAVFAQSS
ECCCCCCEECCCCCEEEECCHHHHHHCCCCEEEEECCCHHHHHCCCHHHHHHHEEEECCC
GLTPIVCVGETLEQRERGEADRVITRQVEQGLENTDPSNLIVAYEPIWAIGTGKTCEAED
CCCEEEECCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCH
ANKICSLIRKLIGFDDVIIQYGGSVKPNNIDEIMSMSDIDGVLVGGASLDPNSFARIANY
HHHHHHHHHHHHCCCCEEHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHCCC
Q
C
>Mature Secondary Structure
MRKSVIAGNWKMHMTCAEAKSYLEEFIPLIKNIKDDRKVVIAPPFTAISTFSDHSDFEYL
CCCCEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHHHCCCCCCCCEEE
DISSQNIHWEDQGAFTAEISPKMLLEHGVSYAIVGHSEPRKYFSESDEQINKRAVFAQSS
ECCCCCCEECCCCCEEEECCHHHHHHCCCCEEEEECCCHHHHHCCCHHHHHHHEEEECCC
GLTPIVCVGETLEQRERGEADRVITRQVEQGLENTDPSNLIVAYEPIWAIGTGKTCEAED
CCCEEEECCCHHHHHHCCCHHHHHHHHHHHCCCCCCCCCEEEEECCEEEECCCCCCCCCH
ANKICSLIRKLIGFDDVIIQYGGSVKPNNIDEIMSMSDIDGVLVGGASLDPNSFARIANY
HHHHHHHHHHHHCCCCEEHCCCCCCCCCCHHHHHHHCCCCEEEEECCCCCHHHHHHHCCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA