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The map label for this gene is lig [H]

Identifier: 126696121

GI number: 126696121

Start: 680210

End: 681850

Strand: Reverse

Name: lig [H]

Synonym: P9301_07831

Alternate gene names: 126696121

Gene position: 681850-680210 (Counterclockwise)

Preceding gene: 126696122

Following gene: 126696120

Centisome position: 41.53

GC content: 27.18

Gene sequence:

>1641_bases
ATGAGCTTAAAAAAGTTTTCAGAATTATTTGGCGATCTAGATTCAATTAATAGTACAAATAATAAAATTGAAGTTTTAAA
GAATTATTTTTTATCTAATGATCCAATAGATAACTCATGGGCAATATATTTGCTAACTGGAAAAAGTAATAAGAGATTTA
TTAGTGGAAGATATTTGAAAAATCTTTTTTCTCACATATATGAATATCCTCAATGGTTAATTGATACATGTTATTTAAAA
GTTGGTGATTCTGCTGAGGTAATAACATTATTACTTAAAAATAAAACTAATTCTAGAAAAAAGAAATTGTCAAATATAAG
TCTCACTGAATTACTAAGCGAAACAATACCTGAATTATCAAAACTGAATGACGAGCAGAAAAATTTAGAAATTAAAAATC
TTTGGGAAAAATTACCTGAAGATAACCATCTAATTTTTAATAAAATTCTTACAGGAACTTTTAGAGTAGGAGTCTCTATC
GGATTAATTACAAAATCGATATCAAAACTAACTCATATTGAGGAAGAGATTATTTCTCATAGGTTGATGGGTGATTTTAA
ACCTTCAATTGATTCATATGAATTTTTAATTAACAAGAATATCAATCTTCAAGAGTTAAATTCCAAACCATTTCCATTTC
TTCTAGCAAATACTATTGAAGATAAAATCTTCAAAAATTCAATAAATGATTTTCAATTTGAATGGAAATACGACGGTATA
AGGATGCAATTAATTAAAAGATCAGGCAATGTTTCGTTATGGACAAGAGGGCAAGAATTAGTAAATGAATCTTTCCCAGA
ATTAGTAGAGAAAATGTCAAATATAAAAGATGATTTTGTTCTTGATGGGGAATTATTAGTTTGGAATTTTAAAGAACAAA
TTGCCTTTGATTTTTCTTTACTTCAAAAAAGAATAAATAGAAAGTCTCCTACTAAATCAATCCAAATAAAATATCCAATT
ATTTTTATTGCTTATGATCTTTTAGAGATTAATGGGAGAGATATAAGAGAAATTAAATTAGAAAATAGAAGAATTGAGTT
AGAAAAATATTTTTCAAAATGGCAAATTAAAACTGAGAATAATATCTCTGATATTTTCAAAATATGTGATTTAATCTTTC
CAAAAGATTGGCCTGATGCTTTAGCCTATAAAGAAAAATCTCGAGAAAATAATACTGAAGGATTAATAATTAAGAAAAAG
ACTTCTATATACTCCTCTGGTAGAAAGAAAGGTATTTGGTGGAAATATAAAGTTGATCCTATGCAACTGGATGCTGTTCT
AATTTACGCTAAGGGCGGTAGCGGTAGAAGGGCTGGTCTGTATACAGATTACAGTTTTGCATTATGGAAAGACAAAGAAT
TAATTAAATTTGCAAGTGCATATTCTGGGTTAACGAATATTGAGATTAAAGAGCTAGATAAATGGATAAGAAAAAATACA
ATAGAAAAATTTGGTCCTGTTCGATCGTTAAAACCAGAAATGGTATTCGAAATATCTTTTGAGAAAATACAAATTTCTAA
TCGTCATAAATCAGGCATAGCGGTACGATTTCCAAGAATAACAAAATGGAGAAAAGATAAAAAAATTAAAGATGCAGATA
GCTTAGAGAATGCTTATGAACTAATGAAAAAAATATCATGA

Upstream 100 bases:

>100_bases
AAGCAAAAAATGTATTTTTTCATCATGGAGATAGTGAAGCCTTAAGTAAATATTTAGTTGAAAAGGAATCAATAAATGTC
CTTTTATTTGGAAAATAAAT

Downstream 100 bases:

>100_bases
AAAATATTACGAAAAATAATAAGCAAAATAATTTAATTTCTAAAATTAAACAGTTTTTCTCCACAAATGGATGGGAGCCA
ATACCCTATCAGATCGAATC

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 546; Mature: 545

Protein sequence:

>546_residues
MSLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDPIDNSWAIYLLTGKSNKRFISGRYLKNLFSHIYEYPQWLIDTCYLK
VGDSAEVITLLLKNKTNSRKKKLSNISLTELLSETIPELSKLNDEQKNLEIKNLWEKLPEDNHLIFNKILTGTFRVGVSI
GLITKSISKLTHIEEEIISHRLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSNIKDDFVLDGELLVWNFKEQIAFDFSLLQKRINRKSPTKSIQIKYPI
IFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKTENNISDIFKICDLIFPKDWPDALAYKEKSRENNTEGLIIKKK
TSIYSSGRKKGIWWKYKVDPMQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKIKDADSLENAYELMKKIS

Sequences:

>Translated_546_residues
MSLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDPIDNSWAIYLLTGKSNKRFISGRYLKNLFSHIYEYPQWLIDTCYLK
VGDSAEVITLLLKNKTNSRKKKLSNISLTELLSETIPELSKLNDEQKNLEIKNLWEKLPEDNHLIFNKILTGTFRVGVSI
GLITKSISKLTHIEEEIISHRLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSNIKDDFVLDGELLVWNFKEQIAFDFSLLQKRINRKSPTKSIQIKYPI
IFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKTENNISDIFKICDLIFPKDWPDALAYKEKSRENNTEGLIIKKK
TSIYSSGRKKGIWWKYKVDPMQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKIKDADSLENAYELMKKIS
>Mature_545_residues
SLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDPIDNSWAIYLLTGKSNKRFISGRYLKNLFSHIYEYPQWLIDTCYLKV
GDSAEVITLLLKNKTNSRKKKLSNISLTELLSETIPELSKLNDEQKNLEIKNLWEKLPEDNHLIFNKILTGTFRVGVSIG
LITKSISKLTHIEEEIISHRLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGIR
MQLIKRSGNVSLWTRGQELVNESFPELVEKMSNIKDDFVLDGELLVWNFKEQIAFDFSLLQKRINRKSPTKSIQIKYPII
FIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKTENNISDIFKICDLIFPKDWPDALAYKEKSRENNTEGLIIKKKT
SIYSSGRKKGIWWKYKVDPMQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNTI
EKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKIKDADSLENAYELMKKIS

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=349, Percent_Identity=25.7879656160458, Blast_Score=94, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI71985269, Length=370, Percent_Identity=28.1081081081081, Blast_Score=112, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6320038, Length=347, Percent_Identity=21.9020172910663, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24762424, Length=499, Percent_Identity=22.4448897795591, Blast_Score=98, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 64017; Mature: 63886

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDPIDNSWAIYLLTGKSNKRFISGRYLK
CCHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEECHHHHH
NLFSHIYEYPQWLIDTCYLKVGDSAEVITLLLKNKTNSRKKKLSNISLTELLSETIPELS
HHHHHHHHHHHHHHHHHHEEECCCHHEEHEEHHCCCCHHHHHHCCCHHHHHHHHHHHHHH
KLNDEQKNLEIKNLWEKLPEDNHLIFNKILTGTFRVGVSIGLITKSISKLTHIEEEIISH
HCCCHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
RLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
HHHCCCCCCCCCEEEEEECCCCCEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEECCE
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSNIKDDFVLDGELLVWNFKEQIAFDFSL
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECHHHHHHHHHH
LQKRINRKSPTKSIQIKYPIIFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKTEN
HHHHHCCCCCCCEEEEECCEEEEEEEHHHCCCCCEEEEEECCCEEHHHHHHHHEEEECCC
NISDIFKICDLIFPKDWPDALAYKEKSRENNTEGLIIKKKTSIYSSGRKKGIWWKYKVDP
CHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCEEEEECCC
MQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
EEEEEEEEEEECCCCCCCCEEECCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKIKDADSLENAYE
HHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHH
LMKKIS
HHHHCC
>Mature Secondary Structure 
SLKKFSELFGDLDSINSTNNKIEVLKNYFLSNDPIDNSWAIYLLTGKSNKRFISGRYLK
CHHHHHHHHCCHHHCCCCCCHHHHHHHHHCCCCCCCCCEEEEEEECCCCCEEECHHHHH
NLFSHIYEYPQWLIDTCYLKVGDSAEVITLLLKNKTNSRKKKLSNISLTELLSETIPELS
HHHHHHHHHHHHHHHHHHEEECCCHHEEHEEHHCCCCHHHHHHCCCHHHHHHHHHHHHHH
KLNDEQKNLEIKNLWEKLPEDNHLIFNKILTGTFRVGVSIGLITKSISKLTHIEEEIISH
HCCCHHHCCHHHHHHHHCCCCCCEEHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHH
RLMGDFKPSIDSYEFLINKNINLQELNSKPFPFLLANTIEDKIFKNSINDFQFEWKYDGI
HHHCCCCCCCCCEEEEEECCCCCEECCCCCCCEEEHHHHHHHHHHCCCCCCEEEEEECCE
RMQLIKRSGNVSLWTRGQELVNESFPELVEKMSNIKDDFVLDGELLVWNFKEQIAFDFSL
EEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHCCCCCEEECCEEEEEECHHHHHHHHHH
LQKRINRKSPTKSIQIKYPIIFIAYDLLEINGRDIREIKLENRRIELEKYFSKWQIKTEN
HHHHHCCCCCCCEEEEECCEEEEEEEHHHCCCCCEEEEEECCCEEHHHHHHHHEEEECCC
NISDIFKICDLIFPKDWPDALAYKEKSRENNTEGLIIKKKTSIYSSGRKKGIWWKYKVDP
CHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCEEEEEECHHHHHCCCCCCCEEEEECCC
MQLDAVLIYAKGGSGRRAGLYTDYSFALWKDKELIKFASAYSGLTNIEIKELDKWIRKNT
EEEEEEEEEEECCCCCCCCEEECCEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHH
IEKFGPVRSLKPEMVFEISFEKIQISNRHKSGIAVRFPRITKWRKDKKIKDADSLENAYE
HHHHCCCCCCCCCEEEEEEEEEEEECCCCCCCEEEECCCHHHHHCCCCCCCHHHHHHHHH
LMKKIS
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA