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The map label for this gene is amiC [H]

Identifier: 126695980

GI number: 126695980

Start: 571091

End: 572176

Strand: Direct

Name: amiC [H]

Synonym: P9301_06421

Alternate gene names: 126695980

Gene position: 571091-572176 (Clockwise)

Preceding gene: 126695979

Following gene: 126695981

Centisome position: 34.78

GC content: 32.87

Gene sequence:

>1086_bases
ATGATAAAGTTTTTAGATAATAAACTTTTTCGTTATTTATCCGTTTTTTTATTTTTAAATTCTTTAATCCTTCCAGTTAA
ATCGTCAAGTGCTCTGGCGGCGTGGGTCCTAAAGAATAATGGGGTTTTAGAATTAAGAACTAAATCAAATACAAATTTAA
AAGCATACTTTCAGAAGTCTAACCAAATATCTGGGGATAGATTCTGGGTAGATTTTCCAGGAGAATTAAAAAATCCCAGA
ATAATAAAAGGTAATGGCCCTATTAAAGAAATTAGATTAGGTAAACCAAATAAGGGTAAAACAAGATTAGTAATTGAATT
TAAAGAAGAAACTTATTTAGAACCTTTGACTTGGAAAATGGTTGGCTTAGATCAAAATAGATGGAGAATCAAACTATTTA
ACCCAAAATATTCATTTAAAAAGATTGGCGAAGGTTTAGTTGAAAAGAAAAGAGGAAATATCAAAGCAAATCAAAATTTT
ATTCATAAGAAGAAAAACAATTATGGTTACTTGAAACTACCAGAGGTAAAACGAAACAAGTTTGAGGTTGTAATCGATCC
AGGGCATGGAGGACCTGATCCAGGAGCAATAGGCATTGGTGGTATTAGAGAAACAGATGTTGTTCTAGAGGTTTCAAAAA
TAGTTAAAAATTTACTTTCTGAGAAAGGTGTCAAGGTTAGGTTGACTAGAACAAATGAAGTCGATTTGGATTTACCTCCA
AGAGTTTCCATTGCTAATAATACGGATGCAGATATCTTTGTAAGTATTCATGCAAATGCCTCAAGAGGTAAAAGAAGGGA
CATAAATGGATTGGAAACCTTTTATTACAGAGGGTGGAGAGGAAGGTTACTCGCAAAAAGAATTCAAAAACAAATCTTAA
TAGTTTCCCCTGGAAGTCCTGATCGAGGAGTTAAACAAGGTCGATTTTATGTGATTAAAAATACTAGGATGCCTGCAGTA
CTTGTAGAAATTGGATTTTTAACGGGAAGATTAGATGCAAGAAGGTTAGAAAAAATAACGCATCGAAAAAGATTAGCCTA
TGCAATTGCAAAAGGCATCCTCGAATATCTTGATAAAGTAGGGTGA

Upstream 100 bases:

>100_bases
AGGGAGCTGCAATAGCTCCTGCTGATAAAGAAAGAGTAAAGAAAATAAGGGAGCAGATGCCAAGCCTTAAACATAGGAAA
AATAAGTTGTTTTCAAAATA

Downstream 100 bases:

>100_bases
AAATTAAAGTAGGTATATTTGATAGCGGAATAGGTGGTTTTACTATCCTTAATTCTTTACTAAAAATACGTAAAGATGTA
GAAGTTTTTTATTTGGCGGA

Product: cell wall hydrolase/autolysin

Products: NA

Alternate protein names: Cell wall hydrolase; Cell wall-associated polypeptide CWBP49; CWBP49; Major autolysin [H]

Number of amino acids: Translated: 361; Mature: 361

Protein sequence:

>361_residues
MIKFLDNKLFRYLSVFLFLNSLILPVKSSSALAAWVLKNNGVLELRTKSNTNLKAYFQKSNQISGDRFWVDFPGELKNPR
IIKGNGPIKEIRLGKPNKGKTRLVIEFKEETYLEPLTWKMVGLDQNRWRIKLFNPKYSFKKIGEGLVEKKRGNIKANQNF
IHKKKNNYGYLKLPEVKRNKFEVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVKNLLSEKGVKVRLTRTNEVDLDLPP
RVSIANNTDADIFVSIHANASRGKRRDINGLETFYYRGWRGRLLAKRIQKQILIVSPGSPDRGVKQGRFYVIKNTRMPAV
LVEIGFLTGRLDARRLEKITHRKRLAYAIAKGILEYLDKVG

Sequences:

>Translated_361_residues
MIKFLDNKLFRYLSVFLFLNSLILPVKSSSALAAWVLKNNGVLELRTKSNTNLKAYFQKSNQISGDRFWVDFPGELKNPR
IIKGNGPIKEIRLGKPNKGKTRLVIEFKEETYLEPLTWKMVGLDQNRWRIKLFNPKYSFKKIGEGLVEKKRGNIKANQNF
IHKKKNNYGYLKLPEVKRNKFEVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVKNLLSEKGVKVRLTRTNEVDLDLPP
RVSIANNTDADIFVSIHANASRGKRRDINGLETFYYRGWRGRLLAKRIQKQILIVSPGSPDRGVKQGRFYVIKNTRMPAV
LVEIGFLTGRLDARRLEKITHRKRLAYAIAKGILEYLDKVG
>Mature_361_residues
MIKFLDNKLFRYLSVFLFLNSLILPVKSSSALAAWVLKNNGVLELRTKSNTNLKAYFQKSNQISGDRFWVDFPGELKNPR
IIKGNGPIKEIRLGKPNKGKTRLVIEFKEETYLEPLTWKMVGLDQNRWRIKLFNPKYSFKKIGEGLVEKKRGNIKANQNF
IHKKKNNYGYLKLPEVKRNKFEVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVKNLLSEKGVKVRLTRTNEVDLDLPP
RVSIANNTDADIFVSIHANASRGKRRDINGLETFYYRGWRGRLLAKRIQKQILIVSPGSPDRGVKQGRFYVIKNTRMPAV
LVEIGFLTGRLDARRLEKITHRKRLAYAIAKGILEYLDKVG

Specific function: Autolysins are involved in some important biological processes such as cell separation, cell-wall turnover, competence for genetic transformation, formation of the flagella - in particular of its basal body - and sporulation. Has a high affinity for teich

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Secreted, cell wall [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1788776, Length=238, Percent_Identity=30.672268907563, Blast_Score=96, Evalue=4e-21,
Organism=Escherichia coli, GI87082163, Length=219, Percent_Identity=29.2237442922374, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1790611, Length=347, Percent_Identity=24.4956772334294, Blast_Score=76, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007253
- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3; PF04122 CW_binding_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 41195; Mature: 41195

Theoretical pI: Translated: 10.97; Mature: 10.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKFLDNKLFRYLSVFLFLNSLILPVKSSSALAAWVLKNNGVLELRTKSNTNLKAYFQKS
CCEEHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCEEEEEECCCCCEEEEEECC
NQISGDRFWVDFPGELKNPRIIKGNGPIKEIRLGKPNKGKTRLVIEFKEETYLEPLTWKM
CCCCCCEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEE
VGLDQNRWRIKLFNPKYSFKKIGEGLVEKKRGNIKANQNFIHKKKNNYGYLKLPEVKRNK
EECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEECCCCHHEECCCCCCEEECCCCCCCC
FEVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVKNLLSEKGVKVRLTRTNEVDLDLPP
EEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
RVSIANNTDADIFVSIHANASRGKRRDINGLETFYYRGWRGRLLAKRIQKQILIVSPGSP
CEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHEECCCCHHHHHHHHCCEEEEECCCCC
DRGVKQGRFYVIKNTRMPAVLVEIGFLTGRLDARRLEKITHRKRLAYAIAKGILEYLDKV
CCCCCCCEEEEEECCCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
G
C
>Mature Secondary Structure
MIKFLDNKLFRYLSVFLFLNSLILPVKSSSALAAWVLKNNGVLELRTKSNTNLKAYFQKS
CCEEHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEEEECCCEEEEEECCCCCEEEEEECC
NQISGDRFWVDFPGELKNPRIIKGNGPIKEIRLGKPNKGKTRLVIEFKEETYLEPLTWKM
CCCCCCEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCEEEEEEECCCCCCCCCEEEE
VGLDQNRWRIKLFNPKYSFKKIGEGLVEKKRGNIKANQNFIHKKKNNYGYLKLPEVKRNK
EECCCCCEEEEEECCCCCHHHHHHHHHHHHCCCEECCCCHHEECCCCCCEEECCCCCCCC
FEVVIDPGHGGPDPGAIGIGGIRETDVVLEVSKIVKNLLSEKGVKVRLTRTNEVDLDLPP
EEEEEECCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCC
RVSIANNTDADIFVSIHANASRGKRRDINGLETFYYRGWRGRLLAKRIQKQILIVSPGSP
CEEECCCCCCEEEEEEECCCCCCCCCCCCCHHHHEECCCCHHHHHHHHCCEEEEECCCCC
DRGVKQGRFYVIKNTRMPAVLVEIGFLTGRLDARRLEKITHRKRLAYAIAKGILEYLDKV
CCCCCCCEEEEEECCCCCEEEEEEEHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
G
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1357079; 1682302; 9384377 [H]