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The map label for this gene is purA

Identifier: 126695871

GI number: 126695871

Start: 463866

End: 465176

Strand: Reverse

Name: purA

Synonym: P9301_05331

Alternate gene names: 126695871

Gene position: 465176-463866 (Counterclockwise)

Preceding gene: 126695872

Following gene: 126695870

Centisome position: 28.33

GC content: 38.14

Gene sequence:

>1311_bases
TTGGCCAATGTTGTTGTAATCGGCGCCCAATGGGGTGACGAGGGAAAAGGTAAAATAACGGATTTACTTAGTCGTTCGGC
CGATGTTGTCGTTCGCTATCAAGGTGGAGTAAATGCAGGTCATACGATAGTCGTAGACGATAAAGTCTTAAAATTACATT
TAATTCCCTCAGGGATACTTTATAAAAATACTTCTTGTCTGATTGGTTCGGGAACTGTTGTAGATCCAAAAATCTTGCTT
AAAGAAATTGACATGTTAATTGATAATGGTATTGATATCTCAGGGTTAAAAATTTCATCAACATCACATGTAACAATGCC
CTACCACCGAATATTAGATGAAGCGATGGAGGCTGATAGAGGTTCAAACAAAATAGGGACAACAGGTCGTGGGATTGGCC
CAACTTATGCGGATAAATCACAAAGAAATGGCATTAGGATAAGAGACTTGCTCAATAAGGAAAGGCTAAGTGATGTGATT
GAAATTCCATTAAGAGAAAAAAACGGTCTACTAGAAAAAATCTATGGCATTAAACCACTTAAATTAGAAGATATTGTTGA
AGAATATCTTGACTATGGGGAAAGATTATCAAAACATGTTGTTGACTGTACGAGGACTATCCATGCAGCCTCAAAAAACA
AGAAGAATATTCTTTTCGAAGGCGCTCAAGGGACTCTACTTGACTTAGACCATGGGACTTATCCTTTTGTTACCTCATCA
AACCCTATATCAGGTGGGGCATGTATTGGAGCTGGAGTTGGTCCAACTTTAATTGATAGAGTCATAGGTGTCGCAAAAGC
TTATACCACAAGAGTAGGTGAAGGGCCATTCCCAACTGAATTACAAGGAAGTATTAATGATCAACTCTGCGATAGAGGCA
GTGAATTTGGAACCACTACTGGGAGAAGGAGAAGATGTGGGTGGTTTGATGGAGTTATTGGTAAATATGCTGTATCTGTG
AATGGTCTTGATTGTTTAGCCGTTACGAAACTTGATGTGTTAGATGAATTAGATGAGATTCAGGTTTGCATTGCATATGA
TCTCGATGGAGAGAAAATAGACTACTTTCCTACAAATTCAGATGAATTAAAAAAATGTAAGCCAATCTTCAAAAAATTAA
AAGGTTGGCAATGTTCAACTGCAGATTGCAGAAAACTATCTGATCTCCCAGAGAATGCCATGAATTATCTAAGATTTTTA
GCTGAATTAATGGAGGTTCCAATTGCCATTGTCTCGTTGGGGGCGAATAGAGATCAAACTATAGTAATTGAAGACCCTAT
ACATGGTCCTAAAAGAGCACTGCTAAGGTAA

Upstream 100 bases:

>100_bases
GGTTCTAAGAGAAAGTTGATACAATTCATTTACTTTTTAAAAGTAAAACAAGATTTTTGAATATATTAAATATTCGCACA
AAAATAATGCTCTTCTAAAA

Downstream 100 bases:

>100_bases
TTTAGTTCCAAATTAATGAAGGAATCCTTTAGACATTCTGAACATAAAAAAGTTGATCTCATTGGTCTGGGCAACGCAAT
AGTAGATATTATTGTAAATA

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNTSCLIGSGTVVDPKILL
KEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVI
EIPLREKNGLLEKIYGIKPLKLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSV
NGLDCLAVTKLDVLDELDEIQVCIAYDLDGEKIDYFPTNSDELKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Sequences:

>Translated_436_residues
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNTSCLIGSGTVVDPKILL
KEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVI
EIPLREKNGLLEKIYGIKPLKLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSV
NGLDCLAVTKLDVLDELDEIQVCIAYDLDGEKIDYFPTNSDELKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFL
AELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR
>Mature_435_residues
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGILYKNTSCLIGSGTVVDPKILLK
EIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADRGSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVIE
IPLREKNGLLEKIYGIKPLKLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSSN
PISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTTGRRRRCGWFDGVIGKYAVSVN
GLDCLAVTKLDVLDELDEIQVCIAYDLDGEKIDYFPTNSDELKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFLA
ELMEVPIAIVSLGANRDQTIVIEDPIHGPKRALLR

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family

Homologues:

Organism=Homo sapiens, GI34577063, Length=426, Percent_Identity=42.2535211267606, Blast_Score=338, Evalue=8e-93,
Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=44.131455399061, Blast_Score=332, Evalue=4e-91,
Organism=Homo sapiens, GI40316944, Length=397, Percent_Identity=42.3173803526448, Blast_Score=290, Evalue=3e-78,
Organism=Escherichia coli, GI1790620, Length=427, Percent_Identity=46.3700234192037, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI25146555, Length=432, Percent_Identity=42.5925925925926, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI25146553, Length=432, Percent_Identity=42.5925925925926, Blast_Score=311, Evalue=5e-85,
Organism=Saccharomyces cerevisiae, GI6324109, Length=431, Percent_Identity=43.6194895591647, Blast_Score=335, Evalue=1e-92,
Organism=Drosophila melanogaster, GI21356033, Length=425, Percent_Identity=42.5882352941177, Blast_Score=338, Evalue=4e-93,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PURA_PROM0 (A3PBN1)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090757.1
- ProteinModelPortal:   A3PBN1
- SMR:   A3PBN1
- STRING:   A3PBN1
- GeneID:   4912247
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_05331
- eggNOG:   COG0104
- HOGENOM:   HBG658237
- OMA:   DYVVRYQ
- ProtClustDB:   PRK01117
- BioCyc:   PMAR167546:P9301ORF_0546-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00011
- InterPro:   IPR018220
- InterPro:   IPR001114
- PANTHER:   PTHR11846
- SMART:   SM00788
- TIGRFAMs:   TIGR00184

Pfam domain/function: PF00709 Adenylsucc_synt

EC number: =6.3.4.4

Molecular weight: Translated: 47725; Mature: 47594

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: ACT_SITE 13-13 ACT_SITE 41-41 BINDING 128-128 BINDING 142-142 BINDING 223-223 BINDING 238-238 BINDING 302-302 BINDING 304-304

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YKNTSCLIGSGTVVDPKILLKEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADR
EECCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVIEIPLREKNGLLEKIYGIKPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
KLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVSVNGLDCLAVTKLDVLDELDEIQVCIAYDLDGEKIDYFPTNS
CCCCCCCHHHHHHHHEEEEECCCEEEEEHHHHHHHHHCCEEEEEEEECCCCEEEECCCCH
DELKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC
>Mature Secondary Structure 
ANVVVIGAQWGDEGKGKITDLLSRSADVVVRYQGGVNAGHTIVVDDKVLKLHLIPSGIL
CCEEEEEECCCCCCCCHHHHHHCCCCCEEEEECCCCCCCCEEEEECCEEEEEECCCCEE
YKNTSCLIGSGTVVDPKILLKEIDMLIDNGIDISGLKISSTSHVTMPYHRILDEAMEADR
EECCEEEEECCCEECHHHHHHHHHHHHHCCCCCCCEEECCCCCEECHHHHHHHHHHHHCC
GSNKIGTTGRGIGPTYADKSQRNGIRIRDLLNKERLSDVIEIPLREKNGLLEKIYGIKPL
CCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCHHHHHHHHHCCCCCCCCHHHHHHCCCCC
KLEDIVEEYLDYGERLSKHVVDCTRTIHAASKNKKNILFEGAQGTLLDLDHGTYPFVTSS
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEECCCCCCCEEECC
NPISGGACIGAGVGPTLIDRVIGVAKAYTTRVGEGPFPTELQGSINDQLCDRGSEFGTTT
CCCCCCCEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
GRRRRCGWFDGVIGKYAVSVNGLDCLAVTKLDVLDELDEIQVCIAYDLDGEKIDYFPTNS
CCCCCCCHHHHHHHHEEEEECCCEEEEEHHHHHHHHHCCEEEEEEEECCCCEEEECCCCH
DELKKCKPIFKKLKGWQCSTADCRKLSDLPENAMNYLRFLAELMEVPIAIVSLGANRDQT
HHHHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEEECCCCCCE
IVIEDPIHGPKRALLR
EEEECCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA