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The map label for this gene is cobM [H]

Identifier: 126695822

GI number: 126695822

Start: 423789

End: 424544

Strand: Reverse

Name: cobM [H]

Synonym: P9301_04841

Alternate gene names: 126695822

Gene position: 424544-423789 (Counterclockwise)

Preceding gene: 126695823

Following gene: 126695821

Centisome position: 25.86

GC content: 32.94

Gene sequence:

>756_bases
ATGGATAATAAAATTTCCTTTATTGGTGTTGGTCCAGGCGATCCAGAATTATTAACTTTAAAAGCATTAAAAAAGATAAA
AATTGCAGATGTCATTATTTGGACTGATTCTCTAATACCTGAAAAGATTTTAGATTTTTCGAAAGAAGGTTCTGAAAAAA
TAAAAACGAGTTCGCTCAACTTAGAGCAGATCACCTCAATTATGATAGAAAAATTTCAGGCAGGGAAAACTGTTGTAAGG
TTGCATGATGGAGACCCTTGCCTTTTTGGAGCAATTAGAGAGCAAATAGAAATTTTAGAAACCGAAAAAATTGAAATCGA
GGTTGTTCCTGGAGTAAGTGCTTTTCAAGTTGCTGCAGCATATCATAAGGCTGAATTAACAATCCCTGACGTAACACAAA
CAATAATTTTAACTAGAGCAGGAGGGCGAACAGGCATGCCCGAAAAAGAATCTCTGAAAGATCTTGCGAAACACAATTCC
TCTATATGTCTTTATCTAAGTGCTAGGCATGTGAAAAGATCTCAAGAAACTTTACTAGAGTTTTACCCTCCAGAGACTAA
AGTGATTGTTGGATTTAGAGTATCTTGGGATGATGGTTGGACATCATTAATAGAATTAAAAGATATGGAAAAATTTTCTA
TTGAGAAAAAACTAATTAGAACAACCATTTACATAATTAGCCCAGCAATTAATAATTCTCAAAAAAGATCTAATCTTTAT
AATCCATCTTATAAGCATCTCTTTAGGAATAAATAA

Upstream 100 bases:

>100_bases
ATTAGGATGGCTCAATTTATAAGTATTTTTCTATTTTCTTCTGGATTAATTGGAATATTTTTTTTAAGATTGAGAACATA
TATTGGGAAAAATAGAAAGA

Downstream 100 bases:

>100_bases
TCTTAAAGTTGATAGAAAAATATTAATTACTATAATTAGTAAAAATTTAATAACTTTGAAAGAAATAAAATCTAACCCAG
AAGACAATACCAAAAGAAAA

Product: putative precorrin-4 C11-methyltransferase

Products: S-adenosyl-L-homocysteine; Precorrin 5

Alternate protein names: Cobalt-precorrin-3 methylase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MDNKISFIGVGPGDPELLTLKALKKIKIADVIIWTDSLIPEKILDFSKEGSEKIKTSSLNLEQITSIMIEKFQAGKTVVR
LHDGDPCLFGAIREQIEILETEKIEIEVVPGVSAFQVAAAYHKAELTIPDVTQTIILTRAGGRTGMPEKESLKDLAKHNS
SICLYLSARHVKRSQETLLEFYPPETKVIVGFRVSWDDGWTSLIELKDMEKFSIEKKLIRTTIYIISPAINNSQKRSNLY
NPSYKHLFRNK

Sequences:

>Translated_251_residues
MDNKISFIGVGPGDPELLTLKALKKIKIADVIIWTDSLIPEKILDFSKEGSEKIKTSSLNLEQITSIMIEKFQAGKTVVR
LHDGDPCLFGAIREQIEILETEKIEIEVVPGVSAFQVAAAYHKAELTIPDVTQTIILTRAGGRTGMPEKESLKDLAKHNS
SICLYLSARHVKRSQETLLEFYPPETKVIVGFRVSWDDGWTSLIELKDMEKFSIEKKLIRTTIYIISPAINNSQKRSNLY
NPSYKHLFRNK
>Mature_251_residues
MDNKISFIGVGPGDPELLTLKALKKIKIADVIIWTDSLIPEKILDFSKEGSEKIKTSSLNLEQITSIMIEKFQAGKTVVR
LHDGDPCLFGAIREQIEILETEKIEIEVVPGVSAFQVAAAYHKAELTIPDVTQTIILTRAGGRTGMPEKESLKDLAKHNS
SICLYLSARHVKRSQETLLEFYPPETKVIVGFRVSWDDGWTSLIELKDMEKFSIEKKLIRTTIYIISPAINNSQKRSNLY
NPSYKHLFRNK

Specific function: Catalyzes the methylation of C-11 in cobalt-precorrin-4 to form cobalt-precorrin-5 [H]

COG id: COG2875

COG function: function code H; Precorrin-4 methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=188, Percent_Identity=31.3829787234043, Blast_Score=97, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6322922, Length=149, Percent_Identity=29.5302013422819, Blast_Score=70, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006362
- InterPro:   IPR003043 [H]

Pfam domain/function: PF00590 TP_methylase [H]

EC number: 2.1.1.133

Molecular weight: Translated: 28345; Mature: 28345

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDNKISFIGVGPGDPELLTLKALKKIKIADVIIWTDSLIPEKILDFSKEGSEKIKTSSLN
CCCCEEEEEECCCCCCEEEHHHHHHEEEEEEEEEECCCCHHHHHHCCCCCCHHHEECCCC
LEQITSIMIEKFQAGKTVVRLHDGDPCLFGAIREQIEILETEKIEIEVVPGVSAFQVAAA
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHH
YHKAELTIPDVTQTIILTRAGGRTGMPEKESLKDLAKHNSSICLYLSARHVKRSQETLLE
HHHCEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
FYPPETKVIVGFRVSWDDGWTSLIELKDMEKFSIEKKLIRTTIYIISPAINNSQKRSNLY
HCCCCCEEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHEEEEEECCCCCCHHHCCCC
NPSYKHLFRNK
CCHHHHHHCCC
>Mature Secondary Structure
MDNKISFIGVGPGDPELLTLKALKKIKIADVIIWTDSLIPEKILDFSKEGSEKIKTSSLN
CCCCEEEEEECCCCCCEEEHHHHHHEEEEEEEEEECCCCHHHHHHCCCCCCHHHEECCCC
LEQITSIMIEKFQAGKTVVRLHDGDPCLFGAIREQIEILETEKIEIEVVPGVSAFQVAAA
HHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCHHHHHHHH
YHKAELTIPDVTQTIILTRAGGRTGMPEKESLKDLAKHNSSICLYLSARHVKRSQETLLE
HHHCEEECCCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHH
FYPPETKVIVGFRVSWDDGWTSLIELKDMEKFSIEKKLIRTTIYIISPAINNSQKRSNLY
HCCCCCEEEEEEEEECCCCCHHHHHHHCCHHHHHHHHHHHHEEEEEECCCCCCHHHCCCC
NPSYKHLFRNK
CCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-adenosyl-L-methionine; Precorrin 4

Specific reaction: S-adenosyl-L-methionine + precorrin-4 = S-adenosyl-L-homocysteine + precorrin-5

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]