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The map label for this gene is ndhB

Identifier: 126695797

GI number: 126695797

Start: 398369

End: 399889

Strand: Reverse

Name: ndhB

Synonym: P9301_04591

Alternate gene names: 126695797

Gene position: 399889-398369 (Counterclockwise)

Preceding gene: 126695803

Following gene: 126695796

Centisome position: 24.36

GC content: 36.88

Gene sequence:

>1521_bases
GTGCCCAACGAAATCTTTACAATTAATTTAAATGCTCAAGCCATAATTCCAGAGGCTTTTATTTTACTAGGTATTGTTGG
AACACTTCTTGTAGATTTAGCTGGAGAAAAAACTGCATCAAAATGGGCCCCATCAATTTGCTATTTATCAATTGGGAGCT
CTCTTCTTAGTTTGACCTTGCAATGGAATAATCCTGTAGAAAGCGCATTCCTTGGGTCCTTTAATTCAGATAATTTAGCG
ATAGCATTTAGAGCAATAATAGCTTTATCAACTTTAGTTTCTTTACTCATAAGCTGGCGTTATACAGAGCAAAGTGGAAG
CCCTATTGGAGAGTTCGCAGCAATAGTTCTTTCGGCAACTCTTGGGGCAATGCTTCTGTGTGGATCTACTGACCTTATAA
GTGTATTTATATCTCTTGAGACTCTATCAGTAGCGAGCTATTTACTTTCTGGTTATCTCAAAAGAGATCCAAGAAGTTCA
GAAGCAGCTTTAAAATACTTACTTGTTGGTTCAGCTGCAGCTGCAGTCTATTTGTATGGCTCCTCTTTTCTGTATGGATT
AAGTGGTTCAACAAACTTAACAACAATTGGTTTAGAGATTATCAATAAGCCTTCATTCATTACCTCTTTAGCTCTTGTAT
TTGTTTTATCAACTGTTGCATTTAAAATAGCTGCTGTTCCTTTTCATCAATGGACTCCTGATGTATATGAGGGATCACCT
ACACCTGTAGTTGCTTTTTTATCTGTTGGTTCGAAAACAGCAGGGTTTGCATTCGCAATAAGAATATTAAGCACTACTTT
CTCTTCTTTCGATGAGGAATGGAAACTTTTATTTACTATTTTGGCCATTTTGAGCATGGCTCTAGGAAATGTTGTAGCTC
TAGCTCAAACCTCAATGAAAAGGATGCTAGCTTACAGTTCTATTGGTCAAGCTGGATTCGTAATGATTGGAATAGTATCT
GGAACACAAGATGGTTTATCAGCTGCTGTATTATATTTGGCAGCATATTTGTTTATGAATTTGGGGGCATTTTCATGTGT
AATACTTTTCTCTTTAAGAACTGGTTCTGATAGGATTCTTGATTACTCAGGACTTTACCAAAAAGATCCTCTCATTACAT
TAGGCTTAAGCCTTTGTCTTCTATCTCTTGGAGGTTTACCTCCAATGTTAGGATTTTTTGGCAAGATATATTTGTTCTTT
GCAGGTTGGGCAAATCATCAGTATCTATTGGTAATCGTTGGATTAGTAACTTCAGTTATATCCATTTATTACTACATTTC
AGTGATCAAAATGATGGTAGTTAAAGAACCACAGGAAGCTTCTGAAATAGTCAAATCATATCCTGAAGTTAATTGGGGAA
TTGCGGGGCTGCCTCCCTTGAGAGTTGCACTTTATACTTGCGTTGCGGTAACTGCTCTTGGAGGAATCCTCTCTAATCCT
CTTTTTAAATTAGCTAACACAGCAGTTTCAGAAACTCCTTTCCTACAAGATGTTATTGCTGCAGCAAATAATATTTCCTA
G

Upstream 100 bases:

>100_bases
TTTGAAAAATTTCAAAAATCATACCCACAAATTATAATCAAGTTAAGATTAACTCTTAGACCCTTACAATCAAACATCTA
ATTTAATCCAATTTAATAAA

Downstream 100 bases:

>100_bases
AAGAATTTTTCAATAAATGTCTAAGAAAGTCGCAAGTTTAGAAAAAATATCTAAAACATATGGAAAAGATGATCTAACTG
TTAAAGCCTTAGACAGTATA

Product: NAD(P)H-quinone oxidoreductase subunit 2

Products: NAD+; ubiquinol

Alternate protein names: NAD(P)H dehydrogenase subunit 2; NADH-plastoquinone oxidoreductase subunit 2; NDH-1, subunit 2

Number of amino acids: Translated: 506; Mature: 505

Protein sequence:

>506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPSICYLSIGSSLLSLTLQWNNPVESAFLGSFNSDNLA
IAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLTTIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVIVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEVNWGIAGLPPLRVALYTCVAVTALGGILSNP
LFKLANTAVSETPFLQDVIAAANNIS

Sequences:

>Translated_506_residues
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPSICYLSIGSSLLSLTLQWNNPVESAFLGSFNSDNLA
IAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSS
EAALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLTTIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVS
GTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFF
AGWANHQYLLVIVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEVNWGIAGLPPLRVALYTCVAVTALGGILSNP
LFKLANTAVSETPFLQDVIAAANNIS
>Mature_505_residues
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPSICYLSIGSSLLSLTLQWNNPVESAFLGSFNSDNLAI
AFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSATLGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSSE
AALKYLLVGSAAAAVYLYGSSFLYGLSGSTNLTTIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSPT
PVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMKRMLAYSSIGQAGFVMIGIVSG
TQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRILDYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFA
GWANHQYLLVIVGLVTSVISIYYYISVIKMMVVKEPQEASEIVKSYPEVNWGIAGLPPLRVALYTCVAVTALGGILSNPL
FKLANTAVSETPFLQDVIAAANNIS

Specific function: NDH-1 shuttles electrons from an unknown electron donor, via FMN and iron-sulfur (Fe-S) centers, to quinones in the respiratory and/or the photosynthetic chain. The immediate electron acceptor for the enzyme in this species is believed to be plastoquinone

COG id: COG1007

COG function: function code C; NADH:ubiquinone oxidoreductase subunit 2 (chain N)

Gene ontology:

Cell location: Cellular thylakoid membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 2 family

Homologues:

Organism=Homo sapiens, GI251831117, Length=367, Percent_Identity=24.5231607629428, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI145693160, Length=386, Percent_Identity=33.419689119171, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788829, Length=446, Percent_Identity=24.4394618834081, Blast_Score=86, Evalue=6e-18,
Organism=Escherichia coli, GI1788831, Length=303, Percent_Identity=27.3927392739274, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1788614, Length=336, Percent_Identity=26.4880952380952, Blast_Score=75, Evalue=1e-14,
Organism=Escherichia coli, GI1788613, Length=360, Percent_Identity=24.4444444444444, Blast_Score=74, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NU2C_PROM0 (A3PBF7)

Other databases:

- EMBL:   CP000576
- RefSeq:   YP_001090683.1
- STRING:   A3PBF7
- GeneID:   4910969
- GenomeReviews:   CP000576_GR
- KEGG:   pmg:P9301_04591
- eggNOG:   COG1007
- HOGENOM:   HBG747830
- OMA:   MDMNTLL
- ProtClustDB:   PRK02504
- BioCyc:   PMAR167546:P9301ORF_0470-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_00445
- InterPro:   IPR010096
- InterPro:   IPR001750
- TIGRFAMs:   TIGR01770

Pfam domain/function: PF00361 Oxidored_q1

EC number: 1.6.5.3

Molecular weight: Translated: 54017; Mature: 53886

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1ca4c000)-; HASH(0x1bb40350)-; HASH(0x1cb0b8ac)-; HASH(0x19cec320)-; HASH(0x1cd71204)-; HASH(0x1d224ff4)-; HASH(0x1ce2b6b8)-; HASH(0x1c729ea4)-; HASH(0x1ccc85b0)-; HASH(0x1d20c350)-; HASH(0x1c83ba04)-; HASH(0x1d233d48)-; HASH(0x1b7c3308)-;

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPSICYLSIGSSLLSLTL
CCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEHHHCCHHEEEEE
QWNNPVESAFLGSFNSDNLAIAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSAT
ECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAVYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEEEC
SSFLYGLSGSTNLTTIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRIL
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCEE
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVIVGLVTSVI
ECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEVNWGIAGLPPLRVALYTCVAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCH
LFKLANTAVSETPFLQDVIAAANNIS
HHHHHHHHHCCCHHHHHHHHHHCCCH
>Mature Secondary Structure 
PNEIFTINLNAQAIIPEAFILLGIVGTLLVDLAGEKTASKWAPSICYLSIGSSLLSLTL
CCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEHHHCCHHEEEEE
QWNNPVESAFLGSFNSDNLAIAFRAIIALSTLVSLLISWRYTEQSGSPIGEFAAIVLSAT
ECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHH
LGAMLLCGSTDLISVFISLETLSVASYLLSGYLKRDPRSSEAALKYLLVGSAAAAVYLYG
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHEEEEEC
SSFLYGLSGSTNLTTIGLEIINKPSFITSLALVFVLSTVAFKIAAVPFHQWTPDVYEGSP
CCEEEECCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
TPVVAFLSVGSKTAGFAFAIRILSTTFSSFDEEWKLLFTILAILSMALGNVVALAQTSMK
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RMLAYSSIGQAGFVMIGIVSGTQDGLSAAVLYLAAYLFMNLGAFSCVILFSLRTGSDRIL
HHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCHHHHHHHEEECCCCCCEE
DYSGLYQKDPLITLGLSLCLLSLGGLPPMLGFFGKIYLFFAGWANHQYLLVIVGLVTSVI
ECCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
SIYYYISVIKMMVVKEPQEASEIVKSYPEVNWGIAGLPPLRVALYTCVAVTALGGILSNP
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCH
LFKLANTAVSETPFLQDVIAAANNIS
HHHHHHHHHCCCHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; H+; ubiquinone

Specific reaction: NADH + H+ + ubiquinone = NAD+ + ubiquinol

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA