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The map label for this gene is aes [H]

Identifier: 126447855

GI number: 126447855

Start: 760776

End: 761660

Strand: Direct

Name: aes [H]

Synonym: BMA10247_A0826

Alternate gene names: 126447855

Gene position: 760776-761660 (Clockwise)

Preceding gene: 126446884

Following gene: 126445936

Centisome position: 32.34

GC content: 71.53

Gene sequence:

>885_bases
ATGGACGCGCGACGCCGCGCAACACACGTCATGGACGCATTCGATTTCCGCCGCCAAATTTCCAGCACGAGCGCCGAAAG
CGCGCGCGTGCGGCCGGCGCTCGCGGTGTCGGACGTCGTGATCGGCGGCTACGCGCAGGACATCGCGCTGCGTCTGTATC
GGCGCCCCGATAAATCCTCGCTGCCGGTGGTGCTTTATTTCCACGGCGGCGGCTTCGTGCGCGGTTCGCTCGACGACGCC
GAGCGCGCCGCGCGCCATCTCGCCGAGCACGTGCCGGCGCTCGTCGTGTCGGTCGATTATTCGCTCGCGCCGCGGCACCC
GTTTCCGGCCGCGCCGGAAGATGCGTACTGCGCGGCGCGCTGGGCCGAGGCCCGCGCGCGCGCGTTCGGCGGCAATCCGC
GCAAGATCGGCGTGGCCGGCCACGACGCGGGCGGCCAGCTCGCGAACTGCCTGGCGTTCATCGCGCGCGATCGCGGCGAG
ATTCGCATCGCCGCGCAGGCGCTGATCGGCCCGATGCTCGATCCGAGCCTCACGCGGCTCGCCGACGCGACGCGTCTCGC
GTCGTCCGACACGAGCGCGCAGGAGTGCGCCGCGTGCTACCGCGCATACCTGCCGCAGCCGTCGCAGCGGATGCATCCGT
ACGCGGCGCCGCTCGAATCGGTGCGCCTCGCGGGGCTGCCGCCGACGCTCGTCGCGACCGCGCAGAACGACGTGCTGCAC
GTCGAGGCGGAAAAGTACGCGAGCTGTCTGATCGACGCGGGCGTGCCGACGCAGGTGGTGCGCTATCGGGACGTGTCGCA
TGCGGCGCTCGCCGATCACGCGAGCGCGCTCGAAGAGGTGGCGCGTTTCTTCCAGTTCCGCTTCAGCGCGGGTGCACCGG
GCTGA

Upstream 100 bases:

>100_bases
GCGACGCAGCCGACGATTGTTCAGGGGACGTGAATTGTGTTGCCGGATTATAGGCATTTATCCCGATGTCTGCCGGACCC
AGAATGCGATCCATCTCGCA

Downstream 100 bases:

>100_bases
CGCCGTTTTCATCCGAATTCAGAACAACCGACAACCTGGGGGTTTTTTCATGGCTATCTTGCGTACTTCCCGTTCGCGGA
TCGCGGCCGCGGCGTTCGCG

Product: putative carboxylesterase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDKSSLPVVLYFHGGGFVRGSLDDA
ERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDRGE
IRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPLESVRLAGLPPTLVATAQNDVLH
VEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG

Sequences:

>Translated_294_residues
MDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDKSSLPVVLYFHGGGFVRGSLDDA
ERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDRGE
IRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPLESVRLAGLPPTLVATAQNDVLH
VEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG
>Mature_294_residues
MDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDKSSLPVVLYFHGGGFVRGSLDDA
ERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAARWAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDRGE
IRIAAQALIGPMLDPSLTRLADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPLESVRLAGLPPTLVATAQNDVLH
VEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=291, Percent_Identity=28.1786941580756, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI68299767, Length=291, Percent_Identity=26.4604810996564, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI21328446, Length=109, Percent_Identity=36.697247706422, Blast_Score=71, Evalue=1e-12,
Organism=Escherichia coli, GI1786682, Length=242, Percent_Identity=30.5785123966942, Blast_Score=83, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI72001146, Length=147, Percent_Identity=31.9727891156463, Blast_Score=68, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 31657; Mature: 31657

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDKSS
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
LPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAAR
CCEEEEEECCCEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHH
WAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDRGEIRIAAQALIGPMLDPSLTRL
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHH
ADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPLESVRLAGLPPTLVATAQNDVLH
HHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHHHEECCCCCCEEEECCCCEEE
VEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG
EEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MDARRRATHVMDAFDFRRQISSTSAESARVRPALAVSDVVIGGYAQDIALRLYRRPDKSS
CCCHHHHHHHHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
LPVVLYFHGGGFVRGSLDDAERAARHLAEHVPALVVSVDYSLAPRHPFPAAPEDAYCAAR
CCEEEEEECCCEEECCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCCCCCHHHHHH
WAEARARAFGGNPRKIGVAGHDAGGQLANCLAFIARDRGEIRIAAQALIGPMLDPSLTRL
HHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCEEEEHHHHHCCCCCHHHHHH
ADATRLASSDTSAQECAACYRAYLPQPSQRMHPYAAPLESVRLAGLPPTLVATAQNDVLH
HHHHHHHCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCHHHHEECCCCCCEEEECCCCEEE
VEAEKYASCLIDAGVPTQVVRYRDVSHAALADHASALEEVARFFQFRFSAGAPG
EEHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA