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The map label for this gene is icaB [H]

Identifier: 126447513

GI number: 126447513

Start: 2274658

End: 2275482

Strand: Direct

Name: icaB [H]

Synonym: BMA10247_A2342

Alternate gene names: 126447513

Gene position: 2274658-2275482 (Clockwise)

Preceding gene: 126446205

Following gene: 126446466

Centisome position: 96.68

GC content: 70.79

Gene sequence:

>825_bases
TTGGCAAGCGCCGCGCGCCCCGCGTTCGCCGGCGCGAACGCGCCCGCCGCCGAACCGCCCACCGATGCGCAGCGCCCCGC
GATCCTCGTCTATCACCGCTTCTCGACGTCCGCGCCGCCCGATTCGATGACCGTGCGCGTCAGCACGTTCGGCGCGCAGC
TCGCGTTCCTGCGCGCGCACGGTTACACGTTCGTGCCGCTGCGCGACGTCGTCGCGTGGGCGGCTTCGCCGTCCGCGCCG
CTGCCGGACAAGGCGATCGCGATCACCGTCGACGACGGCCACCGCTCGGTGTACGAACTGCTGCGCCCGATCGTGCTGCG
CGAGCGGCTGCCCGTCACGCTGTTCATCTATCCGTCCGCGATCTCGAACGCGTCGTATGCGATGACATGGGACGAACTGC
GCGCGTTGCGCGCCACCGGCCGCTTCGATATCGAATCGCACACGTGGTGGCATCCGAACTTCCGCACCGAGCGGCGGCGC
CTCGCACCCGATGCGTTCCGCCGCTTCGCCGCGACCCAGTTCGCCCATTCGCGCGCGCGGCTCGAGCGCGAAATCGGCGG
GCCGGTCGATCTGCTCGCATGGCCGTTCGGCCTCTACGACGATGAGCTGACGTCGCTCGCCGCGCAGGCGGGCTACGTCG
CGGGCTTCACGCTCGACGCGCGCAAGGTTCGTCGCGGCGACGCGCCGCTCGCGCTGCCGCGCTTCCTGATCGTCGACAGT
TGCACGCCGGCCGTGCTGGCGCGGATGCTCGGCGAACGCGGCGACGCGCGTGCCGATTCGCACGCCGATTTACACGTCGA
TTCACACGCGGAGCAATCGCGATGA

Upstream 100 bases:

>100_bases
GGCCCGAACACGTTTCGAACAACCCAAGGAACGGCCCTTGCGACATCCAGGCTCCCGGCTCACCCGATTCCGGCTCGCGG
CGGCGGCCTGCTGTCTCGCA

Downstream 100 bases:

>100_bases
GCACGATCGACGGCCGTCCCCGCACACGGCGCGCGCGCGGCGTTCCACCTAAGCGTCGGCACGAAGACAAGTGCATGCCG
GTCGTGCTCGCATGGACGCT

Product: polysaccharide deacetylase family protein

Products: NA

Alternate protein names: PNAG N-deacetylase; Poly-beta-1,6-GlcNAc N-deacetylase; Biofilm polysaccharide intercellular adhesin deacetylase; Biofilm PIA deacetylase; Intercellular adhesion protein B [H]

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MASAARPAFAGANAPAAEPPTDAQRPAILVYHRFSTSAPPDSMTVRVSTFGAQLAFLRAHGYTFVPLRDVVAWAASPSAP
LPDKAIAITVDDGHRSVYELLRPIVLRERLPVTLFIYPSAISNASYAMTWDELRALRATGRFDIESHTWWHPNFRTERRR
LAPDAFRRFAATQFAHSRARLEREIGGPVDLLAWPFGLYDDELTSLAAQAGYVAGFTLDARKVRRGDAPLALPRFLIVDS
CTPAVLARMLGERGDARADSHADLHVDSHAEQSR

Sequences:

>Translated_274_residues
MASAARPAFAGANAPAAEPPTDAQRPAILVYHRFSTSAPPDSMTVRVSTFGAQLAFLRAHGYTFVPLRDVVAWAASPSAP
LPDKAIAITVDDGHRSVYELLRPIVLRERLPVTLFIYPSAISNASYAMTWDELRALRATGRFDIESHTWWHPNFRTERRR
LAPDAFRRFAATQFAHSRARLEREIGGPVDLLAWPFGLYDDELTSLAAQAGYVAGFTLDARKVRRGDAPLALPRFLIVDS
CTPAVLARMLGERGDARADSHADLHVDSHAEQSR
>Mature_273_residues
ASAARPAFAGANAPAAEPPTDAQRPAILVYHRFSTSAPPDSMTVRVSTFGAQLAFLRAHGYTFVPLRDVVAWAASPSAPL
PDKAIAITVDDGHRSVYELLRPIVLRERLPVTLFIYPSAISNASYAMTWDELRALRATGRFDIESHTWWHPNFRTERRRL
APDAFRRFAATQFAHSRARLEREIGGPVDLLAWPFGLYDDELTSLAAQAGYVAGFTLDARKVRRGDAPLALPRFLIVDSC
TPAVLARMLGERGDARADSHADLHVDSHAEQSR

Specific function: Catalyzes the N-deacetylation of poly-beta-1,6-N-acetyl- D-glucosamine (PNAG, also referred to as PIA), a biofilm adhesin polysaccharide. N-deacetylation is crucial for attachment of the polysaccharide to the bacterial cell surface; it leads to the introd

COG id: COG0726

COG function: function code G; Predicted xylanase/chitin deacetylase

Gene ontology:

Cell location: Secreted, cell wall. Note=Attached to the cell surface [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide deacetylase family [H]

Homologues:

Organism=Escherichia coli, GI1786322, Length=240, Percent_Identity=27.5, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011330
- InterPro:   IPR002509 [H]

Pfam domain/function: PF01522 Polysacc_deac_1 [H]

EC number: NA

Molecular weight: Translated: 30227; Mature: 30096

Theoretical pI: Translated: 9.00; Mature: 9.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASAARPAFAGANAPAAEPPTDAQRPAILVYHRFSTSAPPDSMTVRVSTFGAQLAFLRAH
CCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCEEEEEECC
GYTFVPLRDVVAWAASPSAPLPDKAIAITVDDGHRSVYELLRPIVLRERLPVTLFIYPSA
CEEEEEHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
ISNASYAMTWDELRALRATGRFDIESHTWWHPNFRTERRRLAPDAFRRFAATQFAHSRAR
CCCCEEEEEHHHHHHHHHCCCEECCCCCEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
LEREIGGPVDLLAWPFGLYDDELTSLAAQAGYVAGFTLDARKVRRGDAPLALPRFLIVDS
HHHHHCCCEEHHHCCCCCCHHHHHHHHHHCCEEEEEEECHHHHHCCCCCCCCCCEEEECC
CTPAVLARMLGERGDARADSHADLHVDSHAEQSR
CCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCC
>Mature Secondary Structure 
ASAARPAFAGANAPAAEPPTDAQRPAILVYHRFSTSAPPDSMTVRVSTFGAQLAFLRAH
CCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCEEEEEEECCCEEEEEECC
GYTFVPLRDVVAWAASPSAPLPDKAIAITVDDGHRSVYELLRPIVLRERLPVTLFIYPSA
CEEEEEHHHHHHHHCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCC
ISNASYAMTWDELRALRATGRFDIESHTWWHPNFRTERRRLAPDAFRRFAATQFAHSRAR
CCCCEEEEEHHHHHHHHHCCCEECCCCCEECCCCHHHHHHCCHHHHHHHHHHHHHHHHHH
LEREIGGPVDLLAWPFGLYDDELTSLAAQAGYVAGFTLDARKVRRGDAPLALPRFLIVDS
HHHHHCCCEEHHHCCCCCCHHHHHHHHHHCCEEEEEEECHHHHHCCCCCCCCCCEEEECC
CTPAVLARMLGERGDARADSHADLHVDSHAEQSR
CCHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA