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The map label for this gene is prfA [H]

Identifier: 126447218

GI number: 126447218

Start: 1868957

End: 1870039

Strand: Direct

Name: prfA [H]

Synonym: BMA10247_A1943

Alternate gene names: 126447218

Gene position: 1868957-1870039 (Clockwise)

Preceding gene: 126447754

Following gene: 126446694

Centisome position: 79.44

GC content: 67.31

Gene sequence:

>1083_bases
ATGAAGACGAGCATGCAAAGCAAGCTCGACCAGCTCACAACCCGGCTGGCCGAACTGAACGACCTGTTGAGCCGCGAGAA
CGTCACCGCGGACCTCGACCAGTATCGCAAGCTGACGCGCGAGCACGCGGAGATCGGCCCCGTCGTCGAGCACTACGCGC
AGTGGCGCCAGGCGCGCGCCGACGAACTCGCCGCGCAGGAACTGCTCGCCGACGCCTCGATGCGCGATTTCGCCGAGGAC
GAGCTGCGCGGCGCGCGCGACCGGATGGGCCGCCTCGCGGCCGAGCTGCAGACGATGCTGCTGCCGAAGGACCCGAACGA
CGAGCGCAACATCTTCGTCGAAATCCGCGCGGGCACGGGCGGCGACGAATCGGCACTGTTCGCGGGCAACCTGCTGCGGA
TGTACCTGCGCTACGCGGAGCGGCAGCGCTGGCAGGTCGAGATGATGTCGGAGAGCCCGTCGGATCTCGGCGGCTACAAG
GAAGTGATCGTGCGGATCGCGGGCTACGGCGCGTACTCGCGCCTGAAGTTCGAATCGGGCGGCCACCGCGTGCAGCGCGT
GCCCGCGACCGAGACGCAGGGCCGCATCCATACGTCCGCGTGCACGGTTGCGGTGATGCCCGAGGCCGACGAGATCGGCG
AAGTCGAGATCAATCCGGCCGATTTGCGGATCGACACGTTCCGCGCGTCGGGCGCGGGCGGCCAGCACATCAACAAGACC
GATTCGGCGGTGCGCGTCACGCACATCCCGACGGGAATCGTCGTCGAGTGCCAGGACGACCGCTCGCAGCACAAGAACAA
GGATCGCGCGCTGAAGGTGCTCGCCGCGCGGATCAAGGACAAGCAGTATCACGAGCAGCACGCGAAGGAGGCAGCCACGC
GCAAGAGCCTGATCGGCTCGGGCGACCGCTCCGAGCGGATCCGCACGTACAACTTCCCGCAGGGCCGGATGACCGATCAC
CGGATCAACCTGACGCTGTACAAGCTCGAGCAGATCATGGACGGCGATCTCGACGAGCTGATCGCCGCGCTCGTGAGCGA
GCACCAGGCCGAGCTGCTCGCCTCGCTCGGCGACGCCGAATGA

Upstream 100 bases:

>100_bases
CGCGCCCCGCTCGTCGGACACGTCGGACCGTTAGCGGCGCCCGCCGGCCCGCCTCTCCTTCCCCTTTTCGAATCCTTCCC
TGGGAGCATCGCTCCGCACC

Downstream 100 bases:

>100_bases
CGCCGCCCGCCCCTCGCACGCTCACTCGCGTTCGCGCCACCCGATGAACACGACGAAACCCTCGCCCGCCACCGCCGCCG
AGCTGCTGCGCGCGTCGCCG

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARADELAAQELLADASMRDFAED
ELRGARDRMGRLAAELQTMLLPKDPNDERNIFVEIRAGTGGDESALFAGNLLRMYLRYAERQRWQVEMMSESPSDLGGYK
EVIVRIAGYGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE

Sequences:

>Translated_360_residues
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARADELAAQELLADASMRDFAED
ELRGARDRMGRLAAELQTMLLPKDPNDERNIFVEIRAGTGGDESALFAGNLLRMYLRYAERQRWQVEMMSESPSDLGGYK
EVIVRIAGYGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE
>Mature_360_residues
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARADELAAQELLADASMRDFAED
ELRGARDRMGRLAAELQTMLLPKDPNDERNIFVEIRAGTGGDESALFAGNLLRMYLRYAERQRWQVEMMSESPSDLGGYK
EVIVRIAGYGAYSRLKFESGGHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGSGDRSERIRTYNFPQGRMTDH
RINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=284, Percent_Identity=49.6478873239437, Blast_Score=280, Evalue=2e-75,
Organism=Homo sapiens, GI34577120, Length=371, Percent_Identity=38.0053908355795, Blast_Score=249, Evalue=2e-66,
Organism=Homo sapiens, GI166795305, Length=189, Percent_Identity=47.6190476190476, Blast_Score=184, Evalue=1e-46,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=60.2777777777778, Blast_Score=441, Evalue=1e-125,
Organism=Escherichia coli, GI2367172, Length=331, Percent_Identity=38.0664652567976, Blast_Score=219, Evalue=2e-58,
Organism=Caenorhabditis elegans, GI17542784, Length=281, Percent_Identity=36.2989323843416, Blast_Score=179, Evalue=2e-45,
Organism=Saccharomyces cerevisiae, GI6321295, Length=353, Percent_Identity=40.5099150141643, Blast_Score=244, Evalue=2e-65,
Organism=Drosophila melanogaster, GI19921226, Length=292, Percent_Identity=43.1506849315069, Blast_Score=221, Evalue=7e-58,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40484; Mature: 40484

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: PS00850 GLY_RADICAL_1 ; PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DELAAQELLADASMRDFAEDELRGARDRMGRLAAELQTMLLPKDPNDERNIFVEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGNLLRMYLRYAERQRWQVEMMSESPSDLGGYKEVIVRIAGYGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE
CCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKTSMQSKLDQLTTRLAELNDLLSRENVTADLDQYRKLTREHAEIGPVVEHYAQWRQARA
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
DELAAQELLADASMRDFAEDELRGARDRMGRLAAELQTMLLPKDPNDERNIFVEIRAGTG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCC
GDESALFAGNLLRMYLRYAERQRWQVEMMSESPSDLGGYKEVIVRIAGYGAYSRLKFESG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHEEEECCC
GHRVQRVPATETQGRIHTSACTVAVMPEADEIGEVEINPADLRIDTFRASGAGGQHINKT
CCEEEECCCCCCCCCEEECEEEEEECCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCC
DSAVRVTHIPTGIVVECQDDRSQHKNKDRALKVLAARIKDKQYHEQHAKEAATRKSLIGS
CCEEEEEECCCCEEEEECCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GDRSERIRTYNFPQGRMTDHRINLTLYKLEQIMDGDLDELIAALVSEHQAELLASLGDAE
CCCCCCCEEECCCCCCCCCCEEEEHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA