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The map label for this gene is gacA [H]

Identifier: 126447142

GI number: 126447142

Start: 708879

End: 709523

Strand: Direct

Name: gacA [H]

Synonym: BMA10247_A0766

Alternate gene names: 126447142

Gene position: 708879-709523 (Clockwise)

Preceding gene: 126446805

Following gene: 126447365

Centisome position: 30.13

GC content: 69.61

Gene sequence:

>645_bases
GTGCTCGTCGTCGACAGCCATCCGATCGTGCGCGACGGCATCGAGGTCGTGCTCGGCAAGGCGTATGCGGGAATCGACGT
GGACGGCGCGGCCGACATGGCCGAGGCGAGCGACCGGTGCGGTCGCGATCGTCCGGATCTCGTGCTGTTGGACGTGATGC
TGCCGGGCGAGGATCCGCTGCCGGCGATGGGCGAGATCCTGCGCGCGCACCCGGGCCTGCGGTTCGTCGTCTTCACCGCG
CTCGGCTCGGAGGCGCGCGCGGCGGACGCGATCCGCTTCGGCGCCTCCGGCTATCTGCTGAAGTGGTGCAGCCCGGACGG
CATCGTGCAGGCGATTCGCCGCGTGATGGCGGGGCGCGTTCACATCGATCCGTCGCTCGACGAGGCGAGGGTGGCGTCGG
CGGCCGGCGGCCGCACGCTCGCCTCGCCCGTCGACGAGCTCACGCGGCGCGAGAAGCAGGTGCTCAAGCTGATCGCCGAC
GGGATGCGCAATCGCGAAATCGCGTCGCTGCTGAACATCAGCCCGAAGACGGTCGATTGTCATCGGCAGCGGCTGATGCA
GAAGCTCGGCGCGCGCAACGTCGCGACCGTGATCCACTGGGCGTATCGATACGGATATGCGAACGCGTGCGCGCCGGCGA
TGTAG

Upstream 100 bases:

>100_bases
GCGCTCCGTTCGGGCGATGCGCCGCGCGGGCGGACGCGCAGGACAGCATGCATCGGAGCAACAAGAATCGATTATGGTCG
TAGAGAGAAAGTGTGAAAGG

Downstream 100 bases:

>100_bases
CGCGCGGCGGGCGTGTAGCGCGCGGCGGGCGCGGACGCGAACGCGGCCGCCGCGCGGTGCGGCATGTCGCGCGTGCGGCC
GTCGCTCGGATTCGTGCCGG

Product: DNA-binding response regulator

Products: NA

Alternate protein names: Global activator [H]

Number of amino acids: Translated: 214; Mature: 214

Protein sequence:

>214_residues
MLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPLPAMGEILRAHPGLRFVVFTA
LGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIAD
GMRNREIASLLNISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM

Sequences:

>Translated_214_residues
MLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPLPAMGEILRAHPGLRFVVFTA
LGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIAD
GMRNREIASLLNISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM
>Mature_214_residues
MLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPLPAMGEILRAHPGLRFVVFTA
LGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRVHIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIAD
GMRNREIASLLNISPKTVDCHRQRLMQKLGARNVATVIHWAYRYGYANACAPAM

Specific function: Positively controls the production of the autoinducer N- butyryl-homoserine lactone and the formation of the virulence factors pyocyanine, cyanide, and lipase [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788222, Length=210, Percent_Identity=33.8095238095238, Blast_Score=113, Evalue=8e-27,
Organism=Escherichia coli, GI1786747, Length=206, Percent_Identity=30.0970873786408, Blast_Score=93, Evalue=1e-20,
Organism=Escherichia coli, GI1788521, Length=212, Percent_Identity=32.0754716981132, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790102, Length=195, Percent_Identity=31.7948717948718, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787473, Length=182, Percent_Identity=32.4175824175824, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788712, Length=209, Percent_Identity=27.7511961722488, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1788546, Length=207, Percent_Identity=26.0869565217391, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23154; Mature: 23154

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00622 HTH_LUXR_1 ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPL
CEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
PAMGEILRAHPGLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRV
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCE
HIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIADGMRNREIASLLNISPKTVDC
EECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHH
HRQRLMQKLGARNVATVIHWAYRYGYANACAPAM
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLVVDSHPIVRDGIEVVLGKAYAGIDVDGAADMAEASDRCGRDRPDLVLLDVMLPGEDPL
CEEECCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCC
PAMGEILRAHPGLRFVVFTALGSEARAADAIRFGASGYLLKWCSPDGIVQAIRRVMAGRV
HHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCE
HIDPSLDEARVASAAGGRTLASPVDELTRREKQVLKLIADGMRNREIASLLNISPKTVDC
EECCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCCHHH
HRQRLMQKLGARNVATVIHWAYRYGYANACAPAM
HHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9159518; 10984043 [H]