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The map label for this gene is asnB-2 [H]

Identifier: 126446544

GI number: 126446544

Start: 2136524

End: 2138392

Strand: Reverse

Name: asnB-2 [H]

Synonym: BMA10247_A2198

Alternate gene names: 126446544

Gene position: 2138392-2136524 (Counterclockwise)

Preceding gene: 126445883

Following gene: 126445973

Centisome position: 90.89

GC content: 69.45

Gene sequence:

>1869_bases
ATGTGCGGCATCACCGGATGGATCGATTTCGCCCGCGATCTGCGCAACGAAGCGCCGATCGTCGACACGATGACCGATAC
GCTCGCGCGCCGCGGGCCCGATGCGCGCGGCGTGTGGCTCGACACGCACGCCGCGCTGGGGCACCGGCGGCTGTCGATCA
TCGATCCCGAGCGCGGCGCGCAGCCGATGCTCACGCCCGAGCGCGGGCCGCGCGGCCTGCCGCGCGCGGTCATCTCGTAT
GCGGGCGAAACCTACAACTTCCGCGAATTGCGGGCCGAGCTGGGCGCGCTCGGCCACCGGTTCGACACGCATTGCGATAC
CGAGGTGGTGCTGCGCGCGTATCTCGAATGGGGCGCGGCGTTCGTCGACCGTCTCAACGGGATGTATTCGATCGCGATCT
GGGATACCGCGCGCGACGAACTGCTGCTCGTGCGCGACCGGCTCGGCGTGAAGCCGCTGTTCTACTATCCGACGGCGGAC
GGCGTGATCTTCGGCTCGGAGCCGAAGGCGATCCTCGCGCATCCCGACGTGCGCGCGCGCACGTCGTCGGAGGGGCTTTG
CGATGCGCTGCTGTTCCTGCGCACGCCGGGGCAGGTGCCGTTCTCGGGGATGCGCGAGGTGAAGCCCGGCCATGTGCTGC
GCGTGCGGCGCGGCGGCCTCGCCGAGCAACGCTACTGGGCGCTCGAAGCGCGCCCGCACACCGACGATCTGCCGACGACG
ATCGCGACGATCCGCGCGCTGCTCGACGACATCGTGTCGCGCCAGATGATCTCGGACGTGCCGCTGTGCGCGCTGCTGTC
GGGCGGCGTCGATTCGAGCACCATCGCCGCGCTCGCGCAGAAGCGGCGGCGCGCGAACGGCGGCGGCGCGTTGTCGACAT
TCTGCGTCGATTTCACCGGCCACACGCGCAACTTTCGCGCCGATCCGATTCGCCCGACGGCCGATGCGCCGTTCGCGCTC
GAAGTCGCGCGGCACATCGGCAGCGATCACCATACGATCGAGCTGGACCAGGCCGGGCTGCTCGATCCGCAGGTGCGCGA
AGCGGTGCTGCGCGCGTGGGACTTGCCGTTCAACTTCGCCGATCTCGACGTGTCGCTGCACCGGCTGTTCGCCGAGGTCC
GCAAGCACGCCACCGTCGCGCTATCCGGCGAGGCGGCCGACGAGATCTTCGGCGGCTACCTGTGGTTCTCCGATCCGGCC
GCGCGGCGTGCACACACGTTCCCGTGGCTGAAGCTGGGCGCGCATCGCGGGCTCGATCCGCGCGCGCTGTTTCATCGGTC
GTTCATCGATGCGCTGCGACTCGGCGAATACGAAGCACAGCTCTACCGCGCGGCGCTCGCCGAAGTGCCGCGCCTCGACG
GCGAGAACGCGGAGGACCGCCGCACGCGCGAGCTGCATTACCTGACGCTCACGCGCTGGCTGCCGGTGCTGCTCGACAAG
AAGGACCGGATGGGGATGGCAAGCGGCCTCGAGGGGCGCGTGCCGTTCTGCGATCACCGGCTCGTCGAGTATGTGTTCAA
CATCCCGTGGGCGATGAAGACGTTCAGCGGCCAGGAGAAGGCGCTGCTGCGCGCGGCGGCGGCCGATCTGCTGCCCGAAT
CGGTGCTGAACCGCAAGAAGGCCGCGTATCCGTCGATTCAGGCGCCGGGCTATGACCGCGGCCTGATCGAGCGCCTCAAT
CGCGCGATCGCGGCCGGCCGCGCGCCGCTCGAACCGTTCATCGACGCCGGCGCGCTGCGCGGCCTGACCGAGAAGACCGC
CGCCGGCAGCCTTTCGGAATTCGAACGAATCCTGCTGGAGTCGAGCAGCCGGCTCAACGACTGGCTCGATCTCTATCGTG
TCGAGCTCGACGGCGTGCCGGCGGGCTGA

Upstream 100 bases:

>100_bases
CATGGGCGACTACGACGCGTCGGGCTGGCTGCGCGGCCCCTGGCTGCACGGCTGAAGCGCACGCTCGAACCCCTGTCTTT
TCAACTCAAAAGGAGCAAGC

Downstream 100 bases:

>100_bases
TCCCTTGACGAACATTTGGAGTGAACTGATGCAAGCCATCGAAATCCGCGAGACGGGCGGCCCCGACGTTCTCGAGTACG
TGACGCGCGCCGCGCGCGAG

Product: asparagine synthase (glutamine-hydrolyzing)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 622; Mature: 622

Protein sequence:

>622_residues
MCGITGWIDFARDLRNEAPIVDTMTDTLARRGPDARGVWLDTHAALGHRRLSIIDPERGAQPMLTPERGPRGLPRAVISY
AGETYNFRELRAELGALGHRFDTHCDTEVVLRAYLEWGAAFVDRLNGMYSIAIWDTARDELLLVRDRLGVKPLFYYPTAD
GVIFGSEPKAILAHPDVRARTSSEGLCDALLFLRTPGQVPFSGMREVKPGHVLRVRRGGLAEQRYWALEARPHTDDLPTT
IATIRALLDDIVSRQMISDVPLCALLSGGVDSSTIAALAQKRRRANGGGALSTFCVDFTGHTRNFRADPIRPTADAPFAL
EVARHIGSDHHTIELDQAGLLDPQVREAVLRAWDLPFNFADLDVSLHRLFAEVRKHATVALSGEAADEIFGGYLWFSDPA
ARRAHTFPWLKLGAHRGLDPRALFHRSFIDALRLGEYEAQLYRAALAEVPRLDGENAEDRRTRELHYLTLTRWLPVLLDK
KDRMGMASGLEGRVPFCDHRLVEYVFNIPWAMKTFSGQEKALLRAAAADLLPESVLNRKKAAYPSIQAPGYDRGLIERLN
RAIAAGRAPLEPFIDAGALRGLTEKTAAGSLSEFERILLESSSRLNDWLDLYRVELDGVPAG

Sequences:

>Translated_622_residues
MCGITGWIDFARDLRNEAPIVDTMTDTLARRGPDARGVWLDTHAALGHRRLSIIDPERGAQPMLTPERGPRGLPRAVISY
AGETYNFRELRAELGALGHRFDTHCDTEVVLRAYLEWGAAFVDRLNGMYSIAIWDTARDELLLVRDRLGVKPLFYYPTAD
GVIFGSEPKAILAHPDVRARTSSEGLCDALLFLRTPGQVPFSGMREVKPGHVLRVRRGGLAEQRYWALEARPHTDDLPTT
IATIRALLDDIVSRQMISDVPLCALLSGGVDSSTIAALAQKRRRANGGGALSTFCVDFTGHTRNFRADPIRPTADAPFAL
EVARHIGSDHHTIELDQAGLLDPQVREAVLRAWDLPFNFADLDVSLHRLFAEVRKHATVALSGEAADEIFGGYLWFSDPA
ARRAHTFPWLKLGAHRGLDPRALFHRSFIDALRLGEYEAQLYRAALAEVPRLDGENAEDRRTRELHYLTLTRWLPVLLDK
KDRMGMASGLEGRVPFCDHRLVEYVFNIPWAMKTFSGQEKALLRAAAADLLPESVLNRKKAAYPSIQAPGYDRGLIERLN
RAIAAGRAPLEPFIDAGALRGLTEKTAAGSLSEFERILLESSSRLNDWLDLYRVELDGVPAG
>Mature_622_residues
MCGITGWIDFARDLRNEAPIVDTMTDTLARRGPDARGVWLDTHAALGHRRLSIIDPERGAQPMLTPERGPRGLPRAVISY
AGETYNFRELRAELGALGHRFDTHCDTEVVLRAYLEWGAAFVDRLNGMYSIAIWDTARDELLLVRDRLGVKPLFYYPTAD
GVIFGSEPKAILAHPDVRARTSSEGLCDALLFLRTPGQVPFSGMREVKPGHVLRVRRGGLAEQRYWALEARPHTDDLPTT
IATIRALLDDIVSRQMISDVPLCALLSGGVDSSTIAALAQKRRRANGGGALSTFCVDFTGHTRNFRADPIRPTADAPFAL
EVARHIGSDHHTIELDQAGLLDPQVREAVLRAWDLPFNFADLDVSLHRLFAEVRKHATVALSGEAADEIFGGYLWFSDPA
ARRAHTFPWLKLGAHRGLDPRALFHRSFIDALRLGEYEAQLYRAALAEVPRLDGENAEDRRTRELHYLTLTRWLPVLLDK
KDRMGMASGLEGRVPFCDHRLVEYVFNIPWAMKTFSGQEKALLRAAAADLLPESVLNRKKAAYPSIQAPGYDRGLIERLN
RAIAAGRAPLEPFIDAGALRGLTEKTAAGSLSEFERILLESSSRLNDWLDLYRVELDGVPAG

Specific function: Asparagine synthetase involved in sporulation [H]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=419, Percent_Identity=27.6849642004773, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI168229252, Length=419, Percent_Identity=27.6849642004773, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI168229250, Length=419, Percent_Identity=27.6849642004773, Blast_Score=118, Evalue=2e-26,
Organism=Homo sapiens, GI296010848, Length=392, Percent_Identity=27.5510204081633, Blast_Score=114, Evalue=4e-25,
Organism=Homo sapiens, GI296010852, Length=317, Percent_Identity=27.1293375394322, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI296010850, Length=317, Percent_Identity=27.1293375394322, Blast_Score=85, Evalue=2e-16,
Organism=Escherichia coli, GI1786889, Length=427, Percent_Identity=30.4449648711944, Blast_Score=154, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6321563, Length=438, Percent_Identity=27.6255707762557, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6325403, Length=444, Percent_Identity=28.3783783783784, Blast_Score=110, Evalue=7e-25,
Organism=Drosophila melanogaster, GI45553209, Length=545, Percent_Identity=27.8899082568807, Blast_Score=147, Evalue=3e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 69181; Mature: 69181

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCGITGWIDFARDLRNEAPIVDTMTDTLARRGPDARGVWLDTHAALGHRRLSIIDPERGA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHCCCEEEEECCCCCC
QPMLTPERGPRGLPRAVISYAGETYNFRELRAELGALGHRFDTHCDTEVVLRAYLEWGAA
CCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
FVDRLNGMYSIAIWDTARDELLLVRDRLGVKPLFYYPTADGVIFGSEPKAILAHPDVRAR
HHHHCCCEEEEEEECCCCCCEEEEHHHCCCCEEEEECCCCCEEECCCCCEEEECCCCCCC
TSSEGLCDALLFLRTPGQVPFSGMREVKPGHVLRVRRGGLAEQRYWALEARPHTDDLPTT
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCHHH
IATIRALLDDIVSRQMISDVPLCALLSGGVDSSTIAALAQKRRRANGGGALSTFCVDFTG
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECC
HTRNFRADPIRPTADAPFALEVARHIGSDHHTIELDQAGLLDPQVREAVLRAWDLPFNFA
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHH
DLDVSLHRLFAEVRKHATVALSGEAADEIFGGYLWFSDPAARRAHTFPWLKLGAHRGLDP
HHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCEEEECCHHHHHHCCCCHHHHCCCCCCCH
RALFHRSFIDALRLGEYEAQLYRAALAEVPRLDGENAEDRRTRELHYLTLTRWLPVLLDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
KDRMGMASGLEGRVPFCDHRLVEYVFNIPWAMKTFSGQEKALLRAAAADLLPESVLNRKK
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AAYPSIQAPGYDRGLIERLNRAIAAGRAPLEPFIDAGALRGLTEKTAAGSLSEFERILLE
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
SSSRLNDWLDLYRVELDGVPAG
CCHHHHHHHHHHEEEECCCCCC
>Mature Secondary Structure
MCGITGWIDFARDLRNEAPIVDTMTDTLARRGPDARGVWLDTHAALGHRRLSIIDPERGA
CCCCHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEEEHHHHHCCCEEEEECCCCCC
QPMLTPERGPRGLPRAVISYAGETYNFRELRAELGALGHRFDTHCDTEVVLRAYLEWGAA
CCCCCCCCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
FVDRLNGMYSIAIWDTARDELLLVRDRLGVKPLFYYPTADGVIFGSEPKAILAHPDVRAR
HHHHCCCEEEEEEECCCCCCEEEEHHHCCCCEEEEECCCCCEEECCCCCEEEECCCCCCC
TSSEGLCDALLFLRTPGQVPFSGMREVKPGHVLRVRRGGLAEQRYWALEARPHTDDLPTT
CCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEECCCCCCCCEEEEECCCCCCCCHHH
IATIRALLDDIVSRQMISDVPLCALLSGGVDSSTIAALAQKRRRANGGGALSTFCVDFTG
HHHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECC
HTRNFRADPIRPTADAPFALEVARHIGSDHHTIELDQAGLLDPQVREAVLRAWDLPFNFA
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEECCCCCCCHHHHHHHHHHHCCCCCHH
DLDVSLHRLFAEVRKHATVALSGEAADEIFGGYLWFSDPAARRAHTFPWLKLGAHRGLDP
HHHHHHHHHHHHHHHCCEEEECCCHHHHHHCCEEEECCHHHHHHCCCCHHHHCCCCCCCH
RALFHRSFIDALRLGEYEAQLYRAALAEVPRLDGENAEDRRTRELHYLTLTRWLPVLLDK
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCC
KDRMGMASGLEGRVPFCDHRLVEYVFNIPWAMKTFSGQEKALLRAAAADLLPESVLNRKK
CCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
AAYPSIQAPGYDRGLIERLNRAIAAGRAPLEPFIDAGALRGLTEKTAAGSLSEFERILLE
HCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHCCCHHHHHHHHHH
SSSRLNDWLDLYRVELDGVPAG
CCHHHHHHHHHHEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10498721 [H]