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The map label for this gene is nirD-2 [H]

Identifier: 126446527

GI number: 126446527

Start: 1214199

End: 1214549

Strand: Reverse

Name: nirD-2 [H]

Synonym: BMA10247_A1248

Alternate gene names: 126446527

Gene position: 1214549-1214199 (Counterclockwise)

Preceding gene: 126447674

Following gene: 126447247

Centisome position: 51.62

GC content: 69.23

Gene sequence:

>351_bases
ATGAACCGCCAACCTCAAGCATCCGCGTGGACGCCCGTCTGCGCGCTCGACGAGATCGTGCCGAACACCGGCGTGTGCGC
GCTCGTCAACGGCGAGCAGATCGCCGTGTTCCACGTCGACGACGGCGGCGGCGGCCGCGCGTTCGCGATCGGCAACGTCG
ATCCGCATTCGCAGGCCGCCGTGCTCTCGCGCGGGCTGATCGGCAGCCTCGGCGAGCGGATCGTCGTCGCATCGCCGCTC
TACAAGCATCATTTCGACCTGCGCACGGGCGAGTGCGTCGAGGCGCCGGAGAAATCGGTGAGCGCGTATCCGTCGCGCGT
CGAGGACGGCTACGTGTGGGTCGCGGCGTGA

Upstream 100 bases:

>100_bases
ACGCCGGACGAGCGCGACGCCGAAGGGCAAGCCGACAGGCAACCCGAAACCGAGACGGCCTGAGCGCGCCGTCTTCGTCA
GTCCAGGCAAGGAGATCGCC

Downstream 100 bases:

>100_bases
CGCGCCGCATCGGCCGATAGCGACGACGCGAAGCGAGGCGAACGTGACGAGCGCGAGCGTGAAGACGGTGTGCCCATACT
GCGGCGTCGGCTGCGGGATG

Product: nitrite reductase [NAD(P)H], small subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 116; Mature: 116

Protein sequence:

>116_residues
MNRQPQASAWTPVCALDEIVPNTGVCALVNGEQIAVFHVDDGGGGRAFAIGNVDPHSQAAVLSRGLIGSLGERIVVASPL
YKHHFDLRTGECVEAPEKSVSAYPSRVEDGYVWVAA

Sequences:

>Translated_116_residues
MNRQPQASAWTPVCALDEIVPNTGVCALVNGEQIAVFHVDDGGGGRAFAIGNVDPHSQAAVLSRGLIGSLGERIVVASPL
YKHHFDLRTGECVEAPEKSVSAYPSRVEDGYVWVAA
>Mature_116_residues
MNRQPQASAWTPVCALDEIVPNTGVCALVNGEQIAVFHVDDGGGGRAFAIGNVDPHSQAAVLSRGLIGSLGERIVVASPL
YKHHFDLRTGECVEAPEKSVSAYPSRVEDGYVWVAA

Specific function: Required for activity of the reductase [H]

COG id: COG2146

COG function: function code PR; Ferredoxin subunits of nitrite reductase and ring-hydroxylating dioxygenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To B.subtilis nasE [H]

Homologues:

Organism=Escherichia coli, GI1789766, Length=108, Percent_Identity=46.2962962962963, Blast_Score=97, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012748
- InterPro:   IPR017881
- InterPro:   IPR017941 [H]

Pfam domain/function: NA

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 12272; Mature: 12272

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRQPQASAWTPVCALDEIVPNTGVCALVNGEQIAVFHVDDGGGGRAFAIGNVDPHSQAA
CCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCEEEEECCCCCCHHHH
VLSRGLIGSLGERIVVASPLYKHHFDLRTGECVEAPEKSVSAYPSRVEDGYVWVAA
HHHCCCHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEC
>Mature Secondary Structure
MNRQPQASAWTPVCALDEIVPNTGVCALVNGEQIAVFHVDDGGGGRAFAIGNVDPHSQAA
CCCCCCCCCCCCHHHHHHHCCCCCEEEEECCCEEEEEEEECCCCCEEEEECCCCCCHHHH
VLSRGLIGSLGERIVVASPLYKHHFDLRTGECVEAPEKSVSAYPSRVEDGYVWVAA
HHHCCCHHCCCCEEEEECCHHHHCCCCCCCCCCCCCCCHHHCCCCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]