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The map label for this gene is emrB [H]

Identifier: 126446405

GI number: 126446405

Start: 1920514

End: 1922100

Strand: Reverse

Name: emrB [H]

Synonym: BMA10247_A1992

Alternate gene names: 126446405

Gene position: 1922100-1920514 (Counterclockwise)

Preceding gene: 126446149

Following gene: 126446952

Centisome position: 81.7

GC content: 70.07

Gene sequence:

>1587_bases
ATGAGCGCGCCGGCGAGACCGGCGGCAGCGGCCGCGAACGGCGCGGGGGCGCACGAGCCGGCCGCGGCGCGCCCGCTGCG
CGGCGCGAAGCTCGCGCTGCTGACGTTCGCGCTGTCGCTCGCGACGTTCATCGAAGTGCTGGACTCGACGGTGGCGAACG
TCGCGGTGCCGGCGATCTCGGGCAGCCTCGGGGTATCGAACAGCCAGGGCACGTGGGTGATCAGCTCGTACTCGGTGGCC
GCGGCGATCGCGGTGCCGCTGACGGGCTGGCTCGCGCGGCGGGTGGGCGAGCAGCGGCTGTTCGTCGCGTCGGTGATTCT
GTTCACGCTGACGTCGCTGCTGTGCGGGCTCGCGCGGGACCTGGAGGTGCTGGTGGCGTGCCGGGCGCTGCAGGGGCTGT
TCTCGGGGCCGATGGTGCCGCTGTCGCAGACGATCCTGATGCGCGCGTTTCCGCCGGCGAAGCGCACGTTCGCGCTGGCG
CTGTGGGGGATGACGGTGCTGCTCGCGCCGATCTTCGGGCCGGTGGTGGGCGGCTGGCTGATCGACAACTTCTCGTGGCC
GTGGATCTTCCTGATCAACCTGCCGATCGGGCTGTTCTCGTTCGCGGTGTGCACGCTGATGCTGCGGCCGCGGGCCTCGC
GCGGCGAGGCGAGCCCGATCGACGTGCCGGGGATCGTGCTGCTGGTGATCGGCGTGGGCTCGCTGCAGGCGATGCTGGAC
CTGGGGCATGACCGGGGGTGGTTCGATTCGTCGCTGATCACGGCGCTGGCGATCGCGGCGGGGGTGTCGCTCGTGTCGCT
GCTGATCTGGGAGCTGGGCGAGGCGCACCCGGTGGTGGAGCTGAGCCTGTTCCGGGAGCGGACCTTCACGTTCTGCGTGG
TGATCATCTCGCTGGGGATGATGAGCTTCTCGGTGGTGGGGGTGGTGTTTCCGCTGTGGCTGCAGGCGGTGATGGGATAC
ACGGCGTACCAGGCGGGGCTGGCGACGGCGTCGATGGGGCTGCTGGCGCTGGTGTTCTCGATCCTGGTGGGGGTGTATGC
GAGCCGGGTGGACGCACGGGTGCTGGTGACGTTCGGGTTCGGGGTGTTCGCGGCGGTGATGGGGTGGAGCACGCACTTCA
CGCTGTCGATGACGTTCGCGCAGGTGGTGACGCCGCGGCTGATCCAGGGGATGGGGCTGCCGTGCTTCTTCATTCCGCTG
ACGGCGGCGACGCTGTCGCGGGTGGCGGACGACAAGCTGGCGGCGGCGTCGAGCCTGTCGAATTTCCTGAGGACGTTGTC
GGCGGCGTTCGGCACGGCGCTGAGCGTGACGTGGTGGGACAACCGGGCGACGTATCACTACGCGGTGGTGTCGCAGGCGG
TGACGCGGGCCTCGGAGAACACGCAGCGGTATGTGGACGCGCTGCACGCGATGGGGCTGCACGGCGCGCGCGAGCTGAGC
TCGCTGCACCAGGTGGTGCGGCAGCAGGCGTACATGATGGCGACGAACGACATGTTCTACATGGCGAGCGTGACGTGCGT
GCTGCTGGCGGGGCTGATGTGGCTGACGCGGCCGAAGCGGGGCGCGGCGGCGACGATGGGGCATTGA

Upstream 100 bases:

>100_bases
TGCGCGCGCGCATGGCGGCGCAGCCCGACGGCTGCGACGAGGCGGCGCGCTTCATCGAGCGCTTCGCGCCGGGCGTCGCG
GCGCGGCAGGCGCAGCCGGC

Downstream 100 bases:

>100_bases
CGGGCGACGTGGCGGTGCGGGGCGCGCGCATGCCGGGTGACGCGCGGCGCCCGCCGCTGAGCGGATGAACAGAGGGCGGC
GGGATCGGCCATGCGCGATG

Product: multidrug resistance protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 528; Mature: 527

Protein sequence:

>528_residues
MSAPARPAAAAANGAGAHEPAAARPLRGAKLALLTFALSLATFIEVLDSTVANVAVPAISGSLGVSNSQGTWVISSYSVA
AAIAVPLTGWLARRVGEQRLFVASVILFTLTSLLCGLARDLEVLVACRALQGLFSGPMVPLSQTILMRAFPPAKRTFALA
LWGMTVLLAPIFGPVVGGWLIDNFSWPWIFLINLPIGLFSFAVCTLMLRPRASRGEASPIDVPGIVLLVIGVGSLQAMLD
LGHDRGWFDSSLITALAIAAGVSLVSLLIWELGEAHPVVELSLFRERTFTFCVVIISLGMMSFSVVGVVFPLWLQAVMGY
TAYQAGLATASMGLLALVFSILVGVYASRVDARVLVTFGFGVFAAVMGWSTHFTLSMTFAQVVTPRLIQGMGLPCFFIPL
TAATLSRVADDKLAAASSLSNFLRTLSAAFGTALSVTWWDNRATYHYAVVSQAVTRASENTQRYVDALHAMGLHGARELS
SLHQVVRQQAYMMATNDMFYMASVTCVLLAGLMWLTRPKRGAAATMGH

Sequences:

>Translated_528_residues
MSAPARPAAAAANGAGAHEPAAARPLRGAKLALLTFALSLATFIEVLDSTVANVAVPAISGSLGVSNSQGTWVISSYSVA
AAIAVPLTGWLARRVGEQRLFVASVILFTLTSLLCGLARDLEVLVACRALQGLFSGPMVPLSQTILMRAFPPAKRTFALA
LWGMTVLLAPIFGPVVGGWLIDNFSWPWIFLINLPIGLFSFAVCTLMLRPRASRGEASPIDVPGIVLLVIGVGSLQAMLD
LGHDRGWFDSSLITALAIAAGVSLVSLLIWELGEAHPVVELSLFRERTFTFCVVIISLGMMSFSVVGVVFPLWLQAVMGY
TAYQAGLATASMGLLALVFSILVGVYASRVDARVLVTFGFGVFAAVMGWSTHFTLSMTFAQVVTPRLIQGMGLPCFFIPL
TAATLSRVADDKLAAASSLSNFLRTLSAAFGTALSVTWWDNRATYHYAVVSQAVTRASENTQRYVDALHAMGLHGARELS
SLHQVVRQQAYMMATNDMFYMASVTCVLLAGLMWLTRPKRGAAATMGH
>Mature_527_residues
SAPARPAAAAANGAGAHEPAAARPLRGAKLALLTFALSLATFIEVLDSTVANVAVPAISGSLGVSNSQGTWVISSYSVAA
AIAVPLTGWLARRVGEQRLFVASVILFTLTSLLCGLARDLEVLVACRALQGLFSGPMVPLSQTILMRAFPPAKRTFALAL
WGMTVLLAPIFGPVVGGWLIDNFSWPWIFLINLPIGLFSFAVCTLMLRPRASRGEASPIDVPGIVLLVIGVGSLQAMLDL
GHDRGWFDSSLITALAIAAGVSLVSLLIWELGEAHPVVELSLFRERTFTFCVVIISLGMMSFSVVGVVFPLWLQAVMGYT
AYQAGLATASMGLLALVFSILVGVYASRVDARVLVTFGFGVFAAVMGWSTHFTLSMTFAQVVTPRLIQGMGLPCFFIPLT
AATLSRVADDKLAAASSLSNFLRTLSAAFGTALSVTWWDNRATYHYAVVSQAVTRASENTQRYVDALHAMGLHGARELSS
LHQVVRQQAYMMATNDMFYMASVTCVLLAGLMWLTRPKRGAAATMGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=496, Percent_Identity=45.5645161290323, Blast_Score=439, Evalue=1e-124,
Organism=Escherichia coli, GI1788710, Length=498, Percent_Identity=44.7791164658635, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1790195, Length=404, Percent_Identity=30.6930693069307, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1788392, Length=401, Percent_Identity=30.6733167082294, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=24.7572815533981, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6322958, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=143, Percent_Identity=27.972027972028, Blast_Score=67, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 56223; Mature: 56092

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPARPAAAAANGAGAHEPAAARPLRGAKLALLTFALSLATFIEVLDSTVANVAVPAIS
CCCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVGEQRLFVASVILFTLTSLLCGLARD
CCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LEVLVACRALQGLFSGPMVPLSQTILMRAFPPAKRTFALALWGMTVLLAPIFGPVVGGWL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNFSWPWIFLINLPIGLFSFAVCTLMLRPRASRGEASPIDVPGIVLLVIGVGSLQAMLD
HCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGHDRGWFDSSLITALAIAAGVSLVSLLIWELGEAHPVVELSLFRERTFTFCVVIISLGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MSFSVVGVVFPLWLQAVMGYTAYQAGLATASMGLLALVFSILVGVYASRVDARVLVTFGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
GVFAAVMGWSTHFTLSMTFAQVVTPRLIQGMGLPCFFIPLTAATLSRVADDKLAAASSLS
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLRTLSAAFGTALSVTWWDNRATYHYAVVSQAVTRASENTQRYVDALHAMGLHGARELS
HHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SLHQVVRQQAYMMATNDMFYMASVTCVLLAGLMWLTRPKRGAAATMGH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SAPARPAAAAANGAGAHEPAAARPLRGAKLALLTFALSLATFIEVLDSTVANVAVPAIS
CCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GSLGVSNSQGTWVISSYSVAAAIAVPLTGWLARRVGEQRLFVASVILFTLTSLLCGLARD
CCCCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHH
LEVLVACRALQGLFSGPMVPLSQTILMRAFPPAKRTFALALWGMTVLLAPIFGPVVGGWL
HHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDNFSWPWIFLINLPIGLFSFAVCTLMLRPRASRGEASPIDVPGIVLLVIGVGSLQAMLD
HCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LGHDRGWFDSSLITALAIAAGVSLVSLLIWELGEAHPVVELSLFRERTFTFCVVIISLGM
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
MSFSVVGVVFPLWLQAVMGYTAYQAGLATASMGLLALVFSILVGVYASRVDARVLVTFGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHH
GVFAAVMGWSTHFTLSMTFAQVVTPRLIQGMGLPCFFIPLTAATLSRVADDKLAAASSLS
HHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
NFLRTLSAAFGTALSVTWWDNRATYHYAVVSQAVTRASENTQRYVDALHAMGLHGARELS
HHHHHHHHHHCCEEEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
SLHQVVRQQAYMMATNDMFYMASVTCVLLAGLMWLTRPKRGAAATMGH
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]