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The map label for this gene is pqqE [H]

Identifier: 126446386

GI number: 126446386

Start: 2064118

End: 2065593

Strand: Reverse

Name: pqqE [H]

Synonym: BMA10247_A2134

Alternate gene names: 126446386

Gene position: 2065593-2064118 (Counterclockwise)

Preceding gene: 126445885

Following gene: 126447647

Centisome position: 87.8

GC content: 69.38

Gene sequence:

>1476_bases
ATGAGCACAACGGACGCGCGCCCGGCCCGCTACCTGTTCGACAGCGACTATCAACGCTTCGTCCCGGTTCATGCCGTGTG
GGAAATCACGCTCGCCTGCGATCTCAAGTGCCTGCACTGCGGCTCGCGGGCCGGCCACCGGCGGACCAACGAACTGAGCA
CGGCCGAATGCCTCGAAGTGATCGACGCGCTCGCGCGGCTCGGCACGCGCGAAGTCTCGCTGATCGGCGGCGAGGCGTAT
CTGCGCAAGGACTGGACGCAACTGATCCGCGCGATCCGCTCGCACGGCATGTATTGCGCGATCCAGACGGGCGGCCGCAA
TCTCACGCCGAAGCGCCTCGCGCAGGCGGTCGACGCGGGCCTGAACGGCGTCGGCGTGTCGCTCGACGGGCTCGCGCCGC
TGCACGACAAGGTGCGCAACGTGCCGGGCGCGTTCGAGCGTGCGCTCGACACGCTGCGCCGCGCGCGCGACGCGGGTATC
GCGGTGAGCGTCAACACGCAGATCGGCGCGCAGACGATGGAGGATCTGCCCGCGCTGATGGACACGATCATCGAGCTCGG
CGCGACGCACTGGCAGATTCAGCTGACGGTCGCGATGGGCAACGCGGTCGACAACGACGAACTGCTGCTGCAGCCGTACC
GGCTCGCCGAGCTGATGCCGCTGCTCGCGAAGCTGTACAAGGACGGCGTGAGCCGCGGGCTGCTGATGACGGTCGGCAAC
AACATCGGCTATTACGGGCCCTACGAGCACCTCTGGCGCGGCTTCGGCGACGAGCGCGTGCACTGGAGCGGCTGCGCGGC
CGGCCAGAACGTGATCGCGCTCGAAGCGGACGGCACCGTGAAGGGCTGCCCGTCGCTCGCGACGGTCGGCTTCTCCGGCG
GCAACGTGCGCGACATGTCGCTCGAGGACATCTGGCGCACGAGCGAGGGCATCCATTTCGGCCGGCTGCGCTCGGTCGAC
GATCTGTGGGGCTTTTGCCGCACCTGCTACTACGCGGACGTGTGCCGCGGCGGCTGCACGTGGACGTCGCATTCGCTGCT
CGGCAAGCCGGGCAACAATCCGTATTGCCACTATCGCGTGCTCGAGCTGCAGAAGCAGGGCCTGCGCGAGCGGATCGCGA
AGGTGCAGGATGCGGGCCCGGCGTCGTTCGCGGTGGGCCGCTTCGATCTCGTGACCGAGCGCATCGCCGACGGCGAGCCG
GTTGCGAGCGTCGTCCGCTCGGGGCAGGTGATCGAGCTCGCGTGGAAGAACCGGGGCAAGCGCTCGCCGGAGGTCGGACG
CGTGCCGCCGAAGCTGAAGATGTGCCGCAACTGCGACGGCTACGTGCATGCGGGCGAACAGACCTGCCCGCACTGCGGCG
GCGACATCGACGCGGCCGCGCGCGCTCACGAGCTCGACGCGCAGCGCCGGCATGCGCTGATGAACGACCTGGAGCGCCTG
CTCGGCCTGCCGGCATCGACGTTCGGCGGCGGGTGA

Upstream 100 bases:

>100_bases
GCGGCTCGAAGCGCGCCGCTGAGCCCGCGCGCGCCGTTTGCCGCACGCGCGGGCCCGTTTCGCGCGTGCGGCCGCCATCG
CCCCATCAGGAGACATCGAC

Downstream 100 bases:

>100_bases
CGCACGCCGTGCGCACCCGCGCACTGGGGCGCGCGCCGCCGGCCGCGCCGGCCGAACGCCGAACGCCGAACGGCCGCAGA
GCCCCTACGCCGCGTGCGTG

Product: radical SAM domain-containing protein

Products: NA

Alternate protein names: Pyrroloquinoline quinone biosynthesis protein E [H]

Number of amino acids: Translated: 491; Mature: 490

Protein sequence:

>491_residues
MSTTDARPARYLFDSDYQRFVPVHAVWEITLACDLKCLHCGSRAGHRRTNELSTAECLEVIDALARLGTREVSLIGGEAY
LRKDWTQLIRAIRSHGMYCAIQTGGRNLTPKRLAQAVDAGLNGVGVSLDGLAPLHDKVRNVPGAFERALDTLRRARDAGI
AVSVNTQIGAQTMEDLPALMDTIIELGATHWQIQLTVAMGNAVDNDELLLQPYRLAELMPLLAKLYKDGVSRGLLMTVGN
NIGYYGPYEHLWRGFGDERVHWSGCAAGQNVIALEADGTVKGCPSLATVGFSGGNVRDMSLEDIWRTSEGIHFGRLRSVD
DLWGFCRTCYYADVCRGGCTWTSHSLLGKPGNNPYCHYRVLELQKQGLRERIAKVQDAGPASFAVGRFDLVTERIADGEP
VASVVRSGQVIELAWKNRGKRSPEVGRVPPKLKMCRNCDGYVHAGEQTCPHCGGDIDAAARAHELDAQRRHALMNDLERL
LGLPASTFGGG

Sequences:

>Translated_491_residues
MSTTDARPARYLFDSDYQRFVPVHAVWEITLACDLKCLHCGSRAGHRRTNELSTAECLEVIDALARLGTREVSLIGGEAY
LRKDWTQLIRAIRSHGMYCAIQTGGRNLTPKRLAQAVDAGLNGVGVSLDGLAPLHDKVRNVPGAFERALDTLRRARDAGI
AVSVNTQIGAQTMEDLPALMDTIIELGATHWQIQLTVAMGNAVDNDELLLQPYRLAELMPLLAKLYKDGVSRGLLMTVGN
NIGYYGPYEHLWRGFGDERVHWSGCAAGQNVIALEADGTVKGCPSLATVGFSGGNVRDMSLEDIWRTSEGIHFGRLRSVD
DLWGFCRTCYYADVCRGGCTWTSHSLLGKPGNNPYCHYRVLELQKQGLRERIAKVQDAGPASFAVGRFDLVTERIADGEP
VASVVRSGQVIELAWKNRGKRSPEVGRVPPKLKMCRNCDGYVHAGEQTCPHCGGDIDAAARAHELDAQRRHALMNDLERL
LGLPASTFGGG
>Mature_490_residues
STTDARPARYLFDSDYQRFVPVHAVWEITLACDLKCLHCGSRAGHRRTNELSTAECLEVIDALARLGTREVSLIGGEAYL
RKDWTQLIRAIRSHGMYCAIQTGGRNLTPKRLAQAVDAGLNGVGVSLDGLAPLHDKVRNVPGAFERALDTLRRARDAGIA
VSVNTQIGAQTMEDLPALMDTIIELGATHWQIQLTVAMGNAVDNDELLLQPYRLAELMPLLAKLYKDGVSRGLLMTVGNN
IGYYGPYEHLWRGFGDERVHWSGCAAGQNVIALEADGTVKGCPSLATVGFSGGNVRDMSLEDIWRTSEGIHFGRLRSVDD
LWGFCRTCYYADVCRGGCTWTSHSLLGKPGNNPYCHYRVLELQKQGLRERIAKVQDAGPASFAVGRFDLVTERIADGEPV
ASVVRSGQVIELAWKNRGKRSPEVGRVPPKLKMCRNCDGYVHAGEQTCPHCGGDIDAAARAHELDAQRRHALMNDLERLL
GLPASTFGGG

Specific function: Together With Moac, Is Involved In The Conversion Of A Guanosine Derivative (Gxp) Into Molybdopterin Precursor Z. [C]

COG id: COG0535

COG function: function code R; Predicted Fe-S oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. PqqE family [H]

Homologues:

Organism=Escherichia coli, GI1786999, Length=145, Percent_Identity=34.4827586206897, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006638
- InterPro:   IPR017200
- InterPro:   IPR011843
- InterPro:   IPR007197 [H]

Pfam domain/function: PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 53738; Mature: 53607

Theoretical pI: Translated: 7.47; Mature: 7.47

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTDARPARYLFDSDYQRFVPVHAVWEITLACDLKCLHCGSRAGHRRTNELSTAECLEV
CCCCCCCCHHHHHCCCHHHHCCHHHHEEEHHHHCCCCEECCCCCCCCCCCCCCHHHHHHH
IDALARLGTREVSLIGGEAYLRKDWTQLIRAIRSHGMYCAIQTGGRNLTPKRLAQAVDAG
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCC
LNGVGVSLDGLAPLHDKVRNVPGAFERALDTLRRARDAGIAVSVNTQIGAQTMEDLPALM
CCCCCEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
DTIIELGATHWQIQLTVAMGNAVDNDELLLQPYRLAELMPLLAKLYKDGVSRGLLMTVGN
HHHHHHCCCEEEEEEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NIGYYGPYEHLWRGFGDERVHWSGCAAGQNVIALEADGTVKGCPSLATVGFSGGNVRDMS
CCCCCCCHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEECCC
LEDIWRTSEGIHFGRLRSVDDLWGFCRTCYYADVCRGGCTWTSHSLLGKPGNNPYCHYRV
HHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEH
LELQKQGLRERIAKVQDAGPASFAVGRFDLVTERIADGEPVASVVRSGQVIELAWKNRGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCC
RSPEVGRVPPKLKMCRNCDGYVHAGEQTCPHCGGDIDAAARAHELDAQRRHALMNDLERL
CCCCCCCCCHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGLPASTFGGG
HCCCCCCCCCC
>Mature Secondary Structure 
STTDARPARYLFDSDYQRFVPVHAVWEITLACDLKCLHCGSRAGHRRTNELSTAECLEV
CCCCCCCHHHHHCCCHHHHCCHHHHEEEHHHHCCCCEECCCCCCCCCCCCCCHHHHHHH
IDALARLGTREVSLIGGEAYLRKDWTQLIRAIRSHGMYCAIQTGGRNLTPKRLAQAVDAG
HHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCC
LNGVGVSLDGLAPLHDKVRNVPGAFERALDTLRRARDAGIAVSVNTQIGAQTMEDLPALM
CCCCCEECCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHH
DTIIELGATHWQIQLTVAMGNAVDNDELLLQPYRLAELMPLLAKLYKDGVSRGLLMTVGN
HHHHHHCCCEEEEEEEEEECCCCCCCCCEECHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NIGYYGPYEHLWRGFGDERVHWSGCAAGQNVIALEADGTVKGCPSLATVGFSGGNVRDMS
CCCCCCCHHHHHCCCCCCCEEECCCCCCCCEEEEECCCCCCCCCCCEEEECCCCCEECCC
LEDIWRTSEGIHFGRLRSVDDLWGFCRTCYYADVCRGGCTWTSHSLLGKPGNNPYCHYRV
HHHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEH
LELQKQGLRERIAKVQDAGPASFAVGRFDLVTERIADGEPVASVVRSGQVIELAWKNRGK
HHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCC
RSPEVGRVPPKLKMCRNCDGYVHAGEQTCPHCGGDIDAAARAHELDAQRRHALMNDLERL
CCCCCCCCCHHHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LGLPASTFGGG
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA