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The map label for this gene is anfA [H]

Identifier: 126446253

GI number: 126446253

Start: 2201559

End: 2202527

Strand: Direct

Name: anfA [H]

Synonym: BMA10247_A2270

Alternate gene names: 126446253

Gene position: 2201559-2202527 (Clockwise)

Preceding gene: 126445644

Following gene: 126446903

Centisome position: 93.58

GC content: 66.46

Gene sequence:

>969_bases
ATGTCGCAACACAGCAGTGACGGCAGACGGCTGCACGGCAATTCGTGTCTGATCCAGGCGCTGTTGCACAAGATCGAAAA
AGTGGCCGCGACTCGTGCAAGCGTGTTGATCGTCGGCGAAAGCGGGGTCGGCAAAGATATCGTCGCAAGACTCCTCCATG
ACATGAGCCCACGCCGCCGTGGCCCGTTCGTTCCGGTCAATTGCGGCGCGATTCCGGCCGACATCGCCGAATCGCAGTTG
TTCGGCCACGAGAAGGGCAGCTTCACCGGCGCGGCCGCGCAGCACATCGGCTTCTTCGAGGCCGCGCGCGGCGGCACGAT
CTTCCTCGACGAAGTGGCCGAGATGTCGCGCGATCTGCAGGTGAAGCTGCTGCGCGCGCTCGAATCGAACACCATCACGC
GCGTGGGCGGCAGCGAGCCGATCGCGCTCGACGTGCGCGTGGTCGCGGCCACTCACCACAACCCTGCCGAGGCGGTGCAG
GAAGGGCGCTTTCGCGAAGACCTGTTCTACCGGCTCGCCGTGTTCGCGCTGCGCGTGCCGGCGCTCAGGCATCGCGAGGA
CGATATCGAGGGCATCGCGCAGGAGCTCGTCGATACGCTCAACAGCCGGCACAGCACGCGCAAGCGGCTCTCGGGGCAGG
CGCTGAAGACGCTGCGCACCTATTCGTGGCCGGGCAACGTGCGCGAGCTGCGCAATGCGATCGAGCGCGCGTACATCCTC
TCCGACGAGCAAATCGAGCAGTTGCCGGTGCGACGGCTCGCGCCGCGCGACGACGTGCGCCGGAACGCGATGACGCTGCC
GCTCGGCATGACGCTCGCGCATTCGCAGCAGCGCTTCATCGCCGCGTCGCTCAAGTACTTCGACGGCGACAAGCCGCGCA
CCGCGAAGGCGCTCGGCATCAGCCTGAAGACGCTGTACAACCGGCTCGCGCTGATGCGCGAGCACGACGGGGAAACCGCG
CAGCACTAA

Upstream 100 bases:

>100_bases
AACGCCGTTCGTTCGCACGCTTGTTTGCTCGCTCATTCGCTCGTTCGCTTCGGAGCCAGTCCGCGCACCTCCCCGGGGAA
CCCCACTCAGCGAGATACCC

Downstream 100 bases:

>100_bases
GGGGGGCGGGCGCGCGGCGGGCGAGCCGCGTCCGGCCGGCGCGCCTCGCGCGGTGAACGGCCGGCGCTCGTCGTGGCTGT
CGCGGCTGTCGCGGCTGTCG

Product: sigma-54 interaction domain/Fis family transcriptional regulator domain-containing protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MSQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFVPVNCGAIPADIAESQL
FGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALDVRVVAATHHNPAEAVQ
EGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWPGNVRELRNAIERAYIL
SDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKTLYNRLALMREHDGETA
QH

Sequences:

>Translated_322_residues
MSQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFVPVNCGAIPADIAESQL
FGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALDVRVVAATHHNPAEAVQ
EGRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWPGNVRELRNAIERAYIL
SDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKTLYNRLALMREHDGETA
QH
>Mature_321_residues
SQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRRGPFVPVNCGAIPADIAESQLF
GHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQVKLLRALESNTITRVGGSEPIALDVRVVAATHHNPAEAVQE
GRFREDLFYRLAVFALRVPALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWPGNVRELRNAIERAYILS
DEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGISLKTLYNRLALMREHDGETAQ
H

Specific function: AnfA is essential for nitrogen fixation under Mo- and V- deficient conditions. It is required for the regulation of nitrogenase 3 transcription. Interacts with sigma-54 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790437, Length=309, Percent_Identity=42.7184466019417, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87082117, Length=226, Percent_Identity=48.2300884955752, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI1788550, Length=305, Percent_Identity=41.3114754098361, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1788905, Length=240, Percent_Identity=45.4166666666667, Blast_Score=213, Evalue=1e-56,
Organism=Escherichia coli, GI1789233, Length=316, Percent_Identity=39.2405063291139, Blast_Score=208, Evalue=4e-55,
Organism=Escherichia coli, GI1786524, Length=318, Percent_Identity=40.8805031446541, Blast_Score=207, Evalue=5e-55,
Organism=Escherichia coli, GI87082152, Length=222, Percent_Identity=50, Blast_Score=204, Evalue=5e-54,
Organism=Escherichia coli, GI1789087, Length=304, Percent_Identity=39.1447368421053, Blast_Score=202, Evalue=3e-53,
Organism=Escherichia coli, GI1790299, Length=241, Percent_Identity=43.9834024896266, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI87081872, Length=322, Percent_Identity=37.888198757764, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1787583, Length=303, Percent_Identity=37.953795379538, Blast_Score=184, Evalue=7e-48,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=37.1681415929204, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081858, Length=307, Percent_Identity=30.2931596091205, Blast_Score=134, Evalue=7e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 35771; Mature: 35640

Theoretical pI: Translated: 10.04; Mature: 10.04

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRR
CCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCC
GPFVPVNCGAIPADIAESQLFGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQ
CCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
VKLLRALESNTITRVGGSEPIALDVRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVP
HHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
ALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWPGNVRELRNAIERAYIL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
SDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGI
CHHHHHHCCHHHCCCHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SLKTLYNRLALMREHDGETAQH
HHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SQHSSDGRRLHGNSCLIQALLHKIEKVAATRASVLIVGESGVGKDIVARLLHDMSPRRR
CCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCEEEEEECCCCCHHHHHHHHHHCCCCCC
GPFVPVNCGAIPADIAESQLFGHEKGSFTGAAAQHIGFFEAARGGTIFLDEVAEMSRDLQ
CCEEEECCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHH
VKLLRALESNTITRVGGSEPIALDVRVVAATHHNPAEAVQEGRFREDLFYRLAVFALRVP
HHHHHHHHCCCEEECCCCCCEEEEEEEEEECCCCHHHHHHCCCHHHHHHHHHHHHHHHHH
ALRHREDDIEGIAQELVDTLNSRHSTRKRLSGQALKTLRTYSWPGNVRELRNAIERAYIL
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHC
SDEQIEQLPVRRLAPRDDVRRNAMTLPLGMTLAHSQQRFIAASLKYFDGDKPRTAKALGI
CHHHHHHCCHHHCCCHHHHHHCCEECCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
SLKTLYNRLALMREHDGETAQH
HHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]