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The map label for this gene is plcB [H]

Identifier: 126446190

GI number: 126446190

Start: 1335677

End: 1337068

Strand: Direct

Name: plcB [H]

Synonym: BMA10247_A1368

Alternate gene names: 126446190

Gene position: 1335677-1337068 (Clockwise)

Preceding gene: 126445834

Following gene: 126446038

Centisome position: 56.77

GC content: 68.32

Gene sequence:

>1392_bases
GTGGCGAAGATCCTCGAAAACGTCGAGCACATCGTCGTCGTGATGTTCGAGAACCGCTCGTTCGACACGATGCTCGGCTG
GCTCTATTCGCCCGACGCGCCGCCCGCGCACGTGCTGCCGCCGCAGAGCTCGCCGCAATTCGACGGCCTGAGGCCCGAGA
TGTCGAATCCGTCGAAGGCGGGCAAGACCATCGCCGTCACGCGCGAGGCGCTCAACAGCGTGATCCCCGATCCGCAGGAG
ACATTCGTCAACGTGACGAGCCAGATCCTCGGCCCGAGCGATGCAGGCAACCCGTGGCCGCCGATGCAGGGCTTCGTCGT
CAATTACGAAACGACGCAGACGACCGATCCGGGCCACGTGATGCAATGCCACAGCGTCGCGCAACTGCCGACGCTCACGG
CGCTCGCGCGCGCGTACGCGGTGTCCGACGCGTGGTTCGCGTCGGTGCCGAGCCAGACCTGGCCGAACCGCGCGTTCGCG
CACGCCGGCACGTCGAACGGGCACGTGAACAACGGCGCGATCCCCGATCCGCTCGAATGGGACGTGCCGACGATCTTCGA
CGTGCTGTCGTCGATCGGCGCGAGCTGGGCGGTGTACAGCGACACGACCGTCACGCCGTCGCTCACGCACACGATGTTCC
CGTCGCTGTGGAAGGATGCATACGCCGAGCGCTTCAGGCGCTTCCCGGATTTCGTCGCCGCGTGCGGAAACGACGCGCTG
CCGCAATACGCGTTCATCGAGCCGAGCTTCCTGTTCGAGCCGAACGACCAGCATCCGCCGCACGACGTGCGCGCGGGCGA
CAAGTTCCTGCACGACATCTGGACGGCGATCCGCACGTCGCCCGGCTGGCCACGCACGCTGCTCGTCGTGACCTATGACG
AGCACGGCGGCTGCTTCGACCACGTGCTGCCGCCCGCGAACGCGACCCCGCCGGACAACGCGAGCAACCCCGGCGATGAG
GCGTTCGGCTTCTATCGCTTCGGCGTGCGGGTGCCGGCCGTCGTCGTGTCGCCGTACATCGAGGCGGGCACCGTGTTCCG
CTCGCCGACGAACGTCCCGTACGATCACACGTCGATCCTCGCGACGCTGCGCGACTGGCTCGATATTCCGCCCGACCGGA
TGCTCGGCAGCGCGCGGATCGCGAACGCGCCGACGCTCGCGCAACTCGTCACACGCGACACGCCGCGCACCGATCTGCCG
ACGATCGCCGCGCCGTCGACCGACTTCGTCCATCCGCCGCCGGATGCGCCGCTCAACGACGTGCAAAAGAGCCTCGTGAC
GGGCACGTCGCGGCGCGACGGGCACGATCCGGCCGCCGGCGTCGCACCGATCGCGACGCGCCAGCACGCGCTCGATTTCT
TCAAGCGCCGGGCGGCGGCAAAAACGGCTTAG

Upstream 100 bases:

>100_bases
ATCCATCCGATTGCGCGAGGACGCGGGGGGCATGCCGCGCCGCCCGGGCGCCCGGACGGGCGACGCAGCCACTCGCGCAC
CGCATGAAAAGGAGAACGCC

Downstream 100 bases:

>100_bases
CGGCTTAGCGGCTTAGCAGCTTGATCGGGCAACGTGGCGGCTGAGCGGTGCAGCGACTCGACGATGTGTCGACTTGCCGC
CCCGACGACCCGACGACCCG

Product: phosphoesterase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNPSKAGKTIAVTREALNSVIPDPQE
TFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNRAFA
HAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLWKDAYAERFRRFPDFVAACGNDAL
PQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGGCFDHVLPPANATPPDNASNPGDE
AFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRTDLP
TIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRRAAAKTA

Sequences:

>Translated_463_residues
MAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNPSKAGKTIAVTREALNSVIPDPQE
TFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNRAFA
HAGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLWKDAYAERFRRFPDFVAACGNDAL
PQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGGCFDHVLPPANATPPDNASNPGDE
AFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRTDLP
TIAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRRAAAKTA
>Mature_462_residues
AKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNPSKAGKTIAVTREALNSVIPDPQET
FVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHVMQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNRAFAH
AGTSNGHVNNGAIPDPLEWDVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLWKDAYAERFRRFPDFVAACGNDALP
QYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGGCFDHVLPPANATPPDNASNPGDEA
FGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSILATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRTDLPT
IAAPSTDFVHPPPDAPLNDVQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRRAAAKTA

Specific function: Hydrolyzes sphingomyelin in addition to phosphatidylcholine. Probable virulence factor implicated in the pathogenesis of M.tuberculosis at the level of intracellular survival, by the alteration of cell signaling events or by direct cytotoxicity [H]

COG id: COG3511

COG function: function code M; Phospholipase C

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial phospholipase C family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007312
- InterPro:   IPR006311 [H]

Pfam domain/function: PF04185 Phosphoesterase [H]

EC number: =3.1.4.3 [H]

Molecular weight: Translated: 50539; Mature: 50408

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNPSKA
CCHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
GKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHV
CCEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHH
MQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNRAFAHAGTSNGHVNNGAIPDPLEW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
DVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLWKDAYAERFRRFPDFVAACGNDAL
CHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGGCFD
CCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHH
HVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSIL
HCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCHHCCCEECCCCCCCCCHHHHH
ATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRTDLPTIAAPSTDFVHPPPDAPLND
HHHHHHHCCCHHHHHCCHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRRAAAKTA
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AKILENVEHIVVVMFENRSFDTMLGWLYSPDAPPAHVLPPQSSPQFDGLRPEMSNPSKA
CHHHHCCCEEEEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHC
GKTIAVTREALNSVIPDPQETFVNVTSQILGPSDAGNPWPPMQGFVVNYETTQTTDPGHV
CCEEEEEHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCHH
MQCHSVAQLPTLTALARAYAVSDAWFASVPSQTWPNRAFAHAGTSNGHVNNGAIPDPLEW
HHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCEECCCCCCCCCCCCCCCCCCCC
DVPTIFDVLSSIGASWAVYSDTTVTPSLTHTMFPSLWKDAYAERFRRFPDFVAACGNDAL
CHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PQYAFIEPSFLFEPNDQHPPHDVRAGDKFLHDIWTAIRTSPGWPRTLLVVTYDEHGGCFD
CCEEEECCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCHH
HVLPPANATPPDNASNPGDEAFGFYRFGVRVPAVVVSPYIEAGTVFRSPTNVPYDHTSIL
HCCCCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHCCCCHHCCCEECCCCCCCCCHHHHH
ATLRDWLDIPPDRMLGSARIANAPTLAQLVTRDTPRTDLPTIAAPSTDFVHPPPDAPLND
HHHHHHHCCCHHHHHCCHHHCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHH
VQKSLVTGTSRRDGHDPAAGVAPIATRQHALDFFKRRAAAKTA
HHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8757862; 9634230; 12218036 [H]