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The map label for this gene is recJ [H]

Identifier: 126207943

GI number: 126207943

Start: 530220

End: 531977

Strand: Direct

Name: recJ [H]

Synonym: APL_0459

Alternate gene names: 126207943

Gene position: 530220-531977 (Clockwise)

Preceding gene: 126207942

Following gene: 126207944

Centisome position: 23.31

GC content: 40.67

Gene sequence:

>1758_bases
ATGATACCGCTTGTTTTATTTATAACGAAAAGAAGAAAAATAGTGCAAAAGCTAATCAAACAACGACCGCCATTAACTTC
GGTCACTCTATCTGAAAATCCTTTATTAAATCGCTTATACCAAAGCAGAGGTATTAATTCCGTTCAGGAATTGGAACGTT
CTTTACAATATTTATATCGTCCGCAGCAACTTGCGAATATAGAACAGGCGGTCAGCCTTTTGATTGATGCATTTAAACAA
CAATCCCGCATTGTAATTGTCGGAGATTTTGATGCGGACGGAGCAACCAGTACGGCATTGACGATTTTGGCATTGCGCCA
ATTAGGGTTTAAAAATGTAGATTATTTAATTCCGGATCGTTTCTCTCAGGGATACGGCTTAAGTGTCGCCGTAGCCGAGA
TGGTATTGGCAAAAGGTGCGGATTTGGTCATTACCGTGGATAACGGTATTTCTTCTTTTGAAGGAATTGCATTATTAAAG
TTGCATCATATAAAAGTATTGGTTACTGATCATCATTTACCGGCGGAAACGTTACCGAATGCGGATGCGATGGTCAATCC
GAATTTAGCCGATTGTTCTTTTCCTTCCAAATCACTGGCCGGCGTGGGGGTTGCTTTTTATGTCATGTTGGCATTACGCA
GTCAAATGCGTGAAGCACAATTATTTTCTTTAAAAGAGCCGAACTTAGCCGAGCTTCTGGATCTTGTCGCATTAGGAACG
GTCGCGGATGTGGTTCCGCTTGATCACAATAACCGAATTCTAGTACATCAAGGATTAACTCGAATTCGTTCGGGACATTG
CCGAGCGGGTATTCGTGCATTAGCGGAAGTATCTAAACGAGAGTTGTCTTCTTTACAAGCCGGCGATTTAGGTTTTGCTA
TCGCACCTCGTTTAAATGCGGCGGGGCGTTTGGAAAATATGTCGCTTGGAGTGGAATTATTAATTGCGGAAAATATGGAG
ATTGCCCGTCAGCTGGCTTTTGAGTTGGACAGCTTAAATCAGACACGTAAAGAATTTGAGCAAGAGATGAAAACCGAGGC
GTTGGCGATTTGCGCAAATTTACCTTCGTTAATGCAAAAAGATCAAGCACACGGTATCGCATTATATCAGGCCGATTGGC
ATCAGGGCGTCATTGGTATTCTAGCTTCGCGTATTAAAGATCAATTTCATCGACCGGTCATTGCATTTGCGCAAGAGAGC
GAAAATAGCGAATATTTAAAAGGATCGGCGCGTTCGATTGCCGGCTTGCATATGCGTGATTTATTAGAACGTATTGATGT
GAAACATCCGAATTTAATCGTGAAATTCGGCGGTCATGCGATGGCGGCGGGGCTAACGATTCATCAAAATAACTTTCTTA
AATTTCAGCATATATTTGATGAAGTGATTGACGAAATAATTGCGCCGGAACAATTACAGGGCGTAATTTATACCGACGGT
GAATTGTCTTCGAACGAATTGAATATTCATATTGCGGAATTATTACAAAAAGCCGGACCTTGGGGACAAAGTTTTCCCGA
ACCGCTCTTTGAAGGAGAGTTTAAAATACTGCAACAAAGAATATTAGGCGGCAAACATTTGAAACTGATGGTAGAGCAGC
CTTTCGGCGGCTTATTTGATGCGATTTGGTTTAATGCGGATTTGCGTATTTTTCCGGATCTATCAATTAAATCGGCTCGA
CTTATTTATAAACTGGATATTAATGAGTTTAAAGGAAATAAAAATTTACAATTAAGAATAGAAAATATCGTTATTTAA

Upstream 100 bases:

>100_bases
ATAGCCGGTTATGTAGAACCTAAAGAATTACTTAAAATGTTAAGCGAATAGCATTAAGTCCTTGAATACAAGCGGTCTGA
TTTTGTAAAATTTTTGCAAA

Downstream 100 bases:

>100_bases
TTAGTTTCGTCAATTTAGTGCTAGAATAAAACATTATTGTATTGTAGAATTAATTATAATCTATATGCCGTATTCAGGTA
TTTAGCATTATATAAAATGA

Product: single-stranded-DNA-specific exonuclease RecJ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MIPLVLFITKRRKIVQKLIKQRPPLTSVTLSENPLLNRLYQSRGINSVQELERSLQYLYRPQQLANIEQAVSLLIDAFKQ
QSRIVIVGDFDADGATSTALTILALRQLGFKNVDYLIPDRFSQGYGLSVAVAEMVLAKGADLVITVDNGISSFEGIALLK
LHHIKVLVTDHHLPAETLPNADAMVNPNLADCSFPSKSLAGVGVAFYVMLALRSQMREAQLFSLKEPNLAELLDLVALGT
VADVVPLDHNNRILVHQGLTRIRSGHCRAGIRALAEVSKRELSSLQAGDLGFAIAPRLNAAGRLENMSLGVELLIAENME
IARQLAFELDSLNQTRKEFEQEMKTEALAICANLPSLMQKDQAHGIALYQADWHQGVIGILASRIKDQFHRPVIAFAQES
ENSEYLKGSARSIAGLHMRDLLERIDVKHPNLIVKFGGHAMAAGLTIHQNNFLKFQHIFDEVIDEIIAPEQLQGVIYTDG
ELSSNELNIHIAELLQKAGPWGQSFPEPLFEGEFKILQQRILGGKHLKLMVEQPFGGLFDAIWFNADLRIFPDLSIKSAR
LIYKLDINEFKGNKNLQLRIENIVI

Sequences:

>Translated_585_residues
MIPLVLFITKRRKIVQKLIKQRPPLTSVTLSENPLLNRLYQSRGINSVQELERSLQYLYRPQQLANIEQAVSLLIDAFKQ
QSRIVIVGDFDADGATSTALTILALRQLGFKNVDYLIPDRFSQGYGLSVAVAEMVLAKGADLVITVDNGISSFEGIALLK
LHHIKVLVTDHHLPAETLPNADAMVNPNLADCSFPSKSLAGVGVAFYVMLALRSQMREAQLFSLKEPNLAELLDLVALGT
VADVVPLDHNNRILVHQGLTRIRSGHCRAGIRALAEVSKRELSSLQAGDLGFAIAPRLNAAGRLENMSLGVELLIAENME
IARQLAFELDSLNQTRKEFEQEMKTEALAICANLPSLMQKDQAHGIALYQADWHQGVIGILASRIKDQFHRPVIAFAQES
ENSEYLKGSARSIAGLHMRDLLERIDVKHPNLIVKFGGHAMAAGLTIHQNNFLKFQHIFDEVIDEIIAPEQLQGVIYTDG
ELSSNELNIHIAELLQKAGPWGQSFPEPLFEGEFKILQQRILGGKHLKLMVEQPFGGLFDAIWFNADLRIFPDLSIKSAR
LIYKLDINEFKGNKNLQLRIENIVI
>Mature_585_residues
MIPLVLFITKRRKIVQKLIKQRPPLTSVTLSENPLLNRLYQSRGINSVQELERSLQYLYRPQQLANIEQAVSLLIDAFKQ
QSRIVIVGDFDADGATSTALTILALRQLGFKNVDYLIPDRFSQGYGLSVAVAEMVLAKGADLVITVDNGISSFEGIALLK
LHHIKVLVTDHHLPAETLPNADAMVNPNLADCSFPSKSLAGVGVAFYVMLALRSQMREAQLFSLKEPNLAELLDLVALGT
VADVVPLDHNNRILVHQGLTRIRSGHCRAGIRALAEVSKRELSSLQAGDLGFAIAPRLNAAGRLENMSLGVELLIAENME
IARQLAFELDSLNQTRKEFEQEMKTEALAICANLPSLMQKDQAHGIALYQADWHQGVIGILASRIKDQFHRPVIAFAQES
ENSEYLKGSARSIAGLHMRDLLERIDVKHPNLIVKFGGHAMAAGLTIHQNNFLKFQHIFDEVIDEIIAPEQLQGVIYTDG
ELSSNELNIHIAELLQKAGPWGQSFPEPLFEGEFKILQQRILGGKHLKLMVEQPFGGLFDAIWFNADLRIFPDLSIKSAR
LIYKLDINEFKGNKNLQLRIENIVI

Specific function: Single-stranded-DNA-specific exonuclease. Required for many types of recombinational events, although the stringency of the requirement for recJ appears to vary with the type of recombinational event monitored and the other recombination gene products whi

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recJ family [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=555, Percent_Identity=58.3783783783784, Blast_Score=663, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1 [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 65118; Mature: 65118

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPLVLFITKRRKIVQKLIKQRPPLTSVTLSENPLLNRLYQSRGINSVQELERSLQYLYR
CCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
PQQLANIEQAVSLLIDAFKQQSRIVIVGDFDADGATSTALTILALRQLGFKNVDYLIPDR
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEECCCC
FSQGYGLSVAVAEMVLAKGADLVITVDNGISSFEGIALLKLHHIKVLVTDHHLPAETLPN
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEEEEEECCCCCHHHCCC
ADAMVNPNLADCSFPSKSLAGVGVAFYVMLALRSQMREAQLFSLKEPNLAELLDLVALGT
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VADVVPLDHNNRILVHQGLTRIRSGHCRAGIRALAEVSKRELSSLQAGDLGFAIAPRLNA
HHHEEECCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
AGRLENMSLGVELLIAENMEIARQLAFELDSLNQTRKEFEQEMKTEALAICANLPSLMQK
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
DQAHGIALYQADWHQGVIGILASRIKDQFHRPVIAFAQESENSEYLKGSARSIAGLHMRD
HCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCHHHHHHHHHHH
LLERIDVKHPNLIVKFGGHAMAAGLTIHQNNFLKFQHIFDEVIDEIIAPEQLQGVIYTDG
HHHHHCCCCCCEEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCEEEEECC
ELSSNELNIHIAELLQKAGPWGQSFPEPLFEGEFKILQQRILGGKHLKLMVEQPFGGLFD
CCCCCCEEEEHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEECCCCHHHH
AIWFNADLRIFPDLSIKSARLIYKLDINEFKGNKNLQLRIENIVI
HHHCCCCEEEECCCCCCCEEEEEEEEHHHHCCCCCEEEEEEEECC
>Mature Secondary Structure
MIPLVLFITKRRKIVQKLIKQRPPLTSVTLSENPLLNRLYQSRGINSVQELERSLQYLYR
CCCEEEEEHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHCCCCHHHHHHHHHHHHHC
PQQLANIEQAVSLLIDAFKQQSRIVIVGDFDADGATSTALTILALRQLGFKNVDYLIPDR
CHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEECCCC
FSQGYGLSVAVAEMVLAKGADLVITVDNGISSFEGIALLKLHHIKVLVTDHHLPAETLPN
CCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEEEEEEEEEEEECCCCCHHHCCC
ADAMVNPNLADCSFPSKSLAGVGVAFYVMLALRSQMREAQLFSLKEPNLAELLDLVALGT
CCCEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
VADVVPLDHNNRILVHQGLTRIRSGHCRAGIRALAEVSKRELSSLQAGDLGFAIAPRLNA
HHHEEECCCCCEEEEECCHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCC
AGRLENMSLGVELLIAENMEIARQLAFELDSLNQTRKEFEQEMKTEALAICANLPSLMQK
CCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
DQAHGIALYQADWHQGVIGILASRIKDQFHRPVIAFAQESENSEYLKGSARSIAGLHMRD
HCCCCEEEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHCCCHHHHHHHHHHH
LLERIDVKHPNLIVKFGGHAMAAGLTIHQNNFLKFQHIFDEVIDEIIAPEQLQGVIYTDG
HHHHHCCCCCCEEEEECCCEEEEEEEEECCCCCHHHHHHHHHHHHHCCHHHHCEEEEECC
ELSSNELNIHIAELLQKAGPWGQSFPEPLFEGEFKILQQRILGGKHLKLMVEQPFGGLFD
CCCCCCEEEEHHHHHHHCCCCCCCCCCHHHCCHHHHHHHHHCCCCEEEEEEECCCCHHHH
AIWFNADLRIFPDLSIKSARLIYKLDINEFKGNKNLQLRIENIVI
HHHCCCCEEEECCCCCCCEEEEEEEEHHHHCCCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]