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The map label for this gene is yafJ [C]

Identifier: 126207924

GI number: 126207924

Start: 496824

End: 497621

Strand: Direct

Name: yafJ [C]

Synonym: APL_0440

Alternate gene names: 126207924

Gene position: 496824-497621 (Clockwise)

Preceding gene: 126207923

Following gene: 126207926

Centisome position: 21.84

GC content: 42.23

Gene sequence:

>798_bases
ATGTGTCAATTACTCGGAATGAATTGTAACACCCCAACGGATATTACCTTCTCCTTTGAGGGATTTCGCCGCCGCGCCGG
CGTAACGGATCAACATACGGACGGTTTCGGTATCGCATTTTTTGAAGGTAAAGGCGTACGTATTTTTCGAGATAATCGAC
CGGGCGCATCTTCGCCGATGGCGGATTTAGTCGGACAATACCATATCAAATCATTTAATGTTATTGCACATATTCGTAAA
GCTACCCGAGGCGATATTAATTTAGAAAATACGCATCCGTTTATTCGAGAGTTATGGGGCGAAAACTGGGTGTTCGCACA
TAACGGAACGGTTGAGGGCGTAGGGGTTTGTGAGGAATGTCATTATCAGCCTATCGGAACGACGGACTCCGAAGCCGCAT
TTTGCTGTTTAGTTTCTCAGCTGAGAGAACGTTTCTTAAAAAAACCGAGCGAGAAAGAAATTTTTGATGCGGTTGTAGAA
ATTACTTCCGAAATTGCCAAACACGGCGTATTTAATTTTATTTTATCGAACGGACATTGGATGATTGCTCGTTGTTCGAC
CAATCTACATTATGTAACACGTAAAGCACCGTTTAGCACGGCATTACGTGATGATGACGTAGAAATTGATTTTAGCAAAT
ACACCACCGCAAATGATAAAGTCACCATTATTACCACGCAGCCGCTTACCAAAAACGAAAAATGGGTCAAAATGAACAAC
GGCGGTTATGCGTTTTTTAAAAACGGTGATTTAATCGAAGAAATTGAAGGCACATTACCTGAACAAACACACGTGTAG

Upstream 100 bases:

>100_bases
TCTATTGAATTATTTTTAGGATTTGCAACAATATATCCGTTAGGCCAGCTAGCCAGCTGGTCTTTCTTTTTTATCACCCT
ACCTAACGAAGAATAAATTT

Downstream 100 bases:

>100_bases
TGTAAAAATTCCATAAAAAAATGACCGCTTGTTATTTATTGCAAGCGGTCTTTTTTTATCTGTTTTTAGCAAACGGAAAT
TAGGTTTCCGTTTGTAATCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 265

Protein sequence:

>265_residues
MCQLLGMNCNTPTDITFSFEGFRRRAGVTDQHTDGFGIAFFEGKGVRIFRDNRPGASSPMADLVGQYHIKSFNVIAHIRK
ATRGDINLENTHPFIRELWGENWVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCCLVSQLRERFLKKPSEKEIFDAVVE
ITSEIAKHGVFNFILSNGHWMIARCSTNLHYVTRKAPFSTALRDDDVEIDFSKYTTANDKVTIITTQPLTKNEKWVKMNN
GGYAFFKNGDLIEEIEGTLPEQTHV

Sequences:

>Translated_265_residues
MCQLLGMNCNTPTDITFSFEGFRRRAGVTDQHTDGFGIAFFEGKGVRIFRDNRPGASSPMADLVGQYHIKSFNVIAHIRK
ATRGDINLENTHPFIRELWGENWVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCCLVSQLRERFLKKPSEKEIFDAVVE
ITSEIAKHGVFNFILSNGHWMIARCSTNLHYVTRKAPFSTALRDDDVEIDFSKYTTANDKVTIITTQPLTKNEKWVKMNN
GGYAFFKNGDLIEEIEGTLPEQTHV
>Mature_265_residues
MCQLLGMNCNTPTDITFSFEGFRRRAGVTDQHTDGFGIAFFEGKGVRIFRDNRPGASSPMADLVGQYHIKSFNVIAHIRK
ATRGDINLENTHPFIRELWGENWVFAHNGTVEGVGVCEECHYQPIGTTDSEAAFCCLVSQLRERFLKKPSEKEIFDAVVE
ITSEIAKHGVFNFILSNGHWMIARCSTNLHYVTRKAPFSTALRDDDVEIDFSKYTTANDKVTIITTQPLTKNEKWVKMNN
GGYAFFKNGDLIEEIEGTLPEQTHV

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=254, Percent_Identity=51.9685039370079, Blast_Score=280, Evalue=8e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 29920; Mature: 29920

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLLGMNCNTPTDITFSFEGFRRRAGVTDQHTDGFGIAFFEGKGVRIFRDNRPGASSPM
CCEEEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCH
ADLVGQYHIKSFNVIAHIRKATRGDINLENTHPFIRELWGENWVFAHNGTVEGVGVCEEC
HHHHHHHHCCHHHHHHHHHHHCCCCCEECCCCHHHHHHCCCCEEEEECCCCCCCCCHHHC
HYQPIGTTDSEAAFCCLVSQLRERFLKKPSEKEIFDAVVEITSEIAKHGVFNFILSNGHW
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
MIARCSTNLHYVTRKAPFSTALRDDDVEIDFSKYTTANDKVTIITTQPLTKNEKWVKMNN
EEEEECCCEEEEEECCCCCCCCCCCCEEEEEHHEECCCCEEEEEECCCCCCCCCEEEECC
GGYAFFKNGDLIEEIEGTLPEQTHV
CCEEEEECCCHHHHHHCCCCCCCCC
>Mature Secondary Structure
MCQLLGMNCNTPTDITFSFEGFRRRAGVTDQHTDGFGIAFFEGKGVRIFRDNRPGASSPM
CCEEEECCCCCCCEEEEEHHHHHHHCCCCCCCCCCEEEEEEECCCEEEEECCCCCCCCCH
ADLVGQYHIKSFNVIAHIRKATRGDINLENTHPFIRELWGENWVFAHNGTVEGVGVCEEC
HHHHHHHHCCHHHHHHHHHHHCCCCCEECCCCHHHHHHCCCCEEEEECCCCCCCCCHHHC
HYQPIGTTDSEAAFCCLVSQLRERFLKKPSEKEIFDAVVEITSEIAKHGVFNFILSNGHW
CCCCCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCE
MIARCSTNLHYVTRKAPFSTALRDDDVEIDFSKYTTANDKVTIITTQPLTKNEKWVKMNN
EEEEECCCEEEEEECCCCCCCCCCCCEEEEEHHEECCCCEEEEEECCCCCCCCCEEEECC
GGYAFFKNGDLIEEIEGTLPEQTHV
CCEEEEECCCHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]