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The map label for this gene is grpE

Identifier: 126207851

GI number: 126207851

Start: 412560

End: 413156

Strand: Reverse

Name: grpE

Synonym: APL_0367

Alternate gene names: 126207851

Gene position: 413156-412560 (Counterclockwise)

Preceding gene: 126207855

Following gene: 126207850

Centisome position: 18.16

GC content: 46.23

Gene sequence:

>597_bases
ATGACAGCTCAAAACGAAAATGCACAAGCTCAAGCCGAACAAGTTGAAGTTGCAAACGAAGCGCAATTAGAACAAACAGC
CGAAATACAACAAGAACAACCTGTCGAAGCTGAATTAGCAGCGGCTTATGCACGTATTAATGAGTTAGAAACTTATGTTG
CGGAAGCGGACAACCGTGAAAAAGATATTCAATTACGTGCGCAGGCGGAAATCCAAAATATCCGCCGCCGTGCCGAACAA
GATGTTGAAAAAGCGCATAAATTCGCACTCGAAAAATTCTCCAAAGAATTACTAACCGTGGTGGATAATCTTGAACGCGG
CTTAAATGCGCTTGATACGGCGGTCACCGATGAAAAAACACAAGCGTTGGTGGACGGAGTGGAAATGACCCATAAAGAAT
TTATCTCGACTCTGGCTAAATTCGGTGTGGAAGCGGTCGGTGTCGTCGGCGAAGCGTTCAATCCGGAAGTACATGAAGCG
ATTTCAATGCAGCCGGCGGAAGGTATCGAAGCAAATCATATCAGCGTGGTTTTACAAAAAGGTTACACCCTACAAGGCCG
CGTACTTCGCCCGGCAATGGTCATGGTTGCCGGTTAA

Upstream 100 bases:

>100_bases
TAATTTATTCACATTACCGCTTTTACCTTGAAATCAGCAAATTTAACGCTATATTAGTAATGATTTTTCATTTATATATT
TGAAACGAAGAGGATTTAAA

Downstream 100 bases:

>100_bases
ATTTTACTCTCGCCCGCTTGCGGGAGAGAGACAGCCGGTGAAGCGTTCAGCGAACCGGCAGAGAGAGGGATAGCGGTCTA
AATTCGATAAAATCAGCAAA

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MTAQNENAQAQAEQVEVANEAQLEQTAEIQQEQPVEAELAAAYARINELETYVAEADNREKDIQLRAQAEIQNIRRRAEQ
DVEKAHKFALEKFSKELLTVVDNLERGLNALDTAVTDEKTQALVDGVEMTHKEFISTLAKFGVEAVGVVGEAFNPEVHEA
ISMQPAEGIEANHISVVLQKGYTLQGRVLRPAMVMVAG

Sequences:

>Translated_198_residues
MTAQNENAQAQAEQVEVANEAQLEQTAEIQQEQPVEAELAAAYARINELETYVAEADNREKDIQLRAQAEIQNIRRRAEQ
DVEKAHKFALEKFSKELLTVVDNLERGLNALDTAVTDEKTQALVDGVEMTHKEFISTLAKFGVEAVGVVGEAFNPEVHEA
ISMQPAEGIEANHISVVLQKGYTLQGRVLRPAMVMVAG
>Mature_197_residues
TAQNENAQAQAEQVEVANEAQLEQTAEIQQEQPVEAELAAAYARINELETYVAEADNREKDIQLRAQAEIQNIRRRAEQD
VEKAHKFALEKFSKELLTVVDNLERGLNALDTAVTDEKTQALVDGVEMTHKEFISTLAKFGVEAVGVVGEAFNPEVHEAI
SMQPAEGIEANHISVVLQKGYTLQGRVLRPAMVMVAG

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=134, Percent_Identity=35.0746268656716, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI40255109, Length=140, Percent_Identity=35, Blast_Score=80, Evalue=1e-15,
Organism=Escherichia coli, GI1788967, Length=153, Percent_Identity=46.4052287581699, Blast_Score=142, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17552458, Length=141, Percent_Identity=35.4609929078014, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=191, Percent_Identity=35.6020942408377, Blast_Score=100, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24653432, Length=134, Percent_Identity=29.1044776119403, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_ACTP2 (A3MZ85)

Other databases:

- EMBL:   CP000569
- RefSeq:   YP_001053076.1
- ProteinModelPortal:   A3MZ85
- SMR:   A3MZ85
- STRING:   A3MZ85
- GeneID:   4849732
- GenomeReviews:   CP000569_GR
- KEGG:   apl:APL_0367
- eggNOG:   COG0576
- HOGENOM:   HBG732630
- OMA:   NVRRRME
- PhylomeDB:   A3MZ85
- ProtClustDB:   PRK14150
- BioCyc:   APLE416269:APL_0367-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 21840; Mature: 21709

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQNENAQAQAEQVEVANEAQLEQTAEIQQEQPVEAELAAAYARINELETYVAEADNRE
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVDNLERGLNALDTAVTDEKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QALVDGVEMTHKEFISTLAKFGVEAVGVVGEAFNPEVHEAISMQPAEGIEANHISVVLQK
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHC
GYTLQGRVLRPAMVMVAG
CCCCCCCCHHHHHHEECC
>Mature Secondary Structure 
TAQNENAQAQAEQVEVANEAQLEQTAEIQQEQPVEAELAAAYARINELETYVAEADNRE
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
KDIQLRAQAEIQNIRRRAEQDVEKAHKFALEKFSKELLTVVDNLERGLNALDTAVTDEKT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
QALVDGVEMTHKEFISTLAKFGVEAVGVVGEAFNPEVHEAISMQPAEGIEANHISVVLQK
HHHHHHHHHHHHHHHHHHHHHCHHHHHHHCCCCCHHHHHHHCCCCCCCCCCHHHHHHHHC
GYTLQGRVLRPAMVMVAG
CCCCCCCCHHHHHHEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA