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The map label for this gene is prfB [H]

Identifier: 126207810

GI number: 126207810

Start: 363351

End: 364256

Strand: Reverse

Name: prfB [H]

Synonym: APL_0326

Alternate gene names: 126207810

Gene position: 364256-363351 (Counterclockwise)

Preceding gene: 126207814

Following gene: 126207809

Centisome position: 16.01

GC content: 44.81

Gene sequence:

>906_bases
ATGGTGGTCAATACGATCCGTACACTAGATCAAGGTGTGGAAGATGTTGAAGGCTTAATCGAATTAGCCGTAGAAGCGGA
AGATGAAGATACGTTTAACGAAGCACAAGCTGAAGCAAACGAATTAGAAGAAAAATTAGCGAAACTTGAATTTCAACGAA
TGTTTAGCGGTCAGCACGATGCGGCAGATTGCTATGTCGATTTACAAGCCGGCTCAGGCGGTACGGAAGCACAAGACTGG
ACGGAAATGCTACTGCGTATGTATTTACGCTGGGCGGAAAGCAAAGGCTTTAAAACCGAATTAATCGAGGTGTCGGACGG
TGATGTTGCCGGATTGAAATCAGCAACCGTGAAAATCACTGGTGAATATGCGTTCGGCTGGTTGCGTACCGAAACCGGTA
TTCACCGTTTGGTGCGTAAATCGCCATTTGATTCGAATAACCGCCGCCATACTTCATTTGCGGCTGCTTTCGTTTATCCG
GAAATTGATGATGATATTGATATCGAAATCAATCCGGCGGATTTACGTATTGACGTGTATCGAGCTTCAGGAGCAGGTGG
TCAGCACGTCAATAAAACCGAATCGGCGGTGCGTATTACTCATATTCCATCCGGTATTGTGGTGCAATGTCAGAACGGTC
GTTCGCAACACCAAAACAAAGATCAATGTATGAAGCAGCTTAAAGCGAAATTATACGAGATGGAAATGATGAAGAAGAAT
GCGGAAAAACAAGCGATGGAAGAAAGTAAATCGGATATCGGCTGGGGCAGCCAAATTCGTTCATACGTATTAGACGACTC
TCGTATCAAAGATTTGCGTACCGGCGTTGAAAATCGCAATACCCAAGCGGTACTTGACGGTGACTTAGACAAATTTATTG
AAGCCAGCCTTAAAGCGGGGCTTTAA

Upstream 100 bases:

>100_bases
CGAACGCTTAGAAGAAGTCAATGCTGAATTAGAACAACCAGAAATTTGGAATACGCCGGAAAAAGCACAAGCGTTAGGTA
AAGAACGCTCTGCGTTAGAA

Downstream 100 bases:

>100_bases
TTACAAGCGGTTGAATTTGTAAAAAAATTTTGCAAATTTGACCGCTTATTTTTTTACTCGGGAGCTAGATTATGTTTTTC
GAATCTTTGCAATTTAGTAT

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVVNTIRTLDQGVEDVEGLIELAVEAEDEDTFNEAQAEANELEEKLAKLEFQRMFSGQHDAADCYVDLQAGSGGTEAQDW
TEMLLRMYLRWAESKGFKTELIEVSDGDVAGLKSATVKITGEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFAAAFVYP
EIDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNGRSQHQNKDQCMKQLKAKLYEMEMMKKN
AEKQAMEESKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKAGL

Sequences:

>Translated_301_residues
MVVNTIRTLDQGVEDVEGLIELAVEAEDEDTFNEAQAEANELEEKLAKLEFQRMFSGQHDAADCYVDLQAGSGGTEAQDW
TEMLLRMYLRWAESKGFKTELIEVSDGDVAGLKSATVKITGEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFAAAFVYP
EIDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNGRSQHQNKDQCMKQLKAKLYEMEMMKKN
AEKQAMEESKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKAGL
>Mature_301_residues
MVVNTIRTLDQGVEDVEGLIELAVEAEDEDTFNEAQAEANELEEKLAKLEFQRMFSGQHDAADCYVDLQAGSGGTEAQDW
TEMLLRMYLRWAESKGFKTELIEVSDGDVAGLKSATVKITGEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFAAAFVYP
EIDDDIDIEINPADLRIDVYRASGAGGQHVNKTESAVRITHIPSGIVVQCQNGRSQHQNKDQCMKQLKAKLYEMEMMKKN
AEKQAMEESKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKAGL

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=314, Percent_Identity=31.8471337579618, Blast_Score=164, Evalue=6e-41,
Organism=Homo sapiens, GI166795303, Length=276, Percent_Identity=34.7826086956522, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI166795305, Length=182, Percent_Identity=36.8131868131868, Blast_Score=116, Evalue=2e-26,
Organism=Escherichia coli, GI2367172, Length=300, Percent_Identity=81, Blast_Score=525, Evalue=1e-150,
Organism=Escherichia coli, GI1787462, Length=243, Percent_Identity=39.917695473251, Blast_Score=184, Evalue=5e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=263, Percent_Identity=32.319391634981, Blast_Score=122, Evalue=3e-28,
Organism=Saccharomyces cerevisiae, GI6321295, Length=249, Percent_Identity=38.5542168674699, Blast_Score=150, Evalue=3e-37,
Organism=Drosophila melanogaster, GI19921226, Length=257, Percent_Identity=36.1867704280156, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 33731; Mature: 33731

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVNTIRTLDQGVEDVEGLIELAVEAEDEDTFNEAQAEANELEEKLAKLEFQRMFSGQHD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTELIEVSDGDVAGLKSATVKIT
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEEE
GEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFAAAFVYPEIDDDIDIEINPADLRIDVY
ECEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEEEEEE
RASGAGGQHVNKTESAVRITHIPSGIVVQCQNGRSQHQNKDQCMKQLKAKLYEMEMMKKN
EECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AEKQAMEESKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKAG
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCC
L
C
>Mature Secondary Structure
MVVNTIRTLDQGVEDVEGLIELAVEAEDEDTFNEAQAEANELEEKLAKLEFQRMFSGQHD
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AADCYVDLQAGSGGTEAQDWTEMLLRMYLRWAESKGFKTELIEVSDGDVAGLKSATVKIT
CCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEEE
GEYAFGWLRTETGIHRLVRKSPFDSNNRRHTSFAAAFVYPEIDDDIDIEINPADLRIDVY
ECEEEEEEEHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCCCCCEEEECCCEEEEEEE
RASGAGGQHVNKTESAVRITHIPSGIVVQCQNGRSQHQNKDQCMKQLKAKLYEMEMMKKN
EECCCCCCCCCCCCCEEEEEECCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
AEKQAMEESKSDIGWGSQIRSYVLDDSRIKDLRTGVENRNTQAVLDGDLDKFIEASLKAG
HHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHHCC
L
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA