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The map label for this gene is hsdS [H]

Identifier: 126207776

GI number: 126207776

Start: 320427

End: 321521

Strand: Direct

Name: hsdS [H]

Synonym: APL_0290

Alternate gene names: 126207776

Gene position: 320427-321521 (Clockwise)

Preceding gene: 126207775

Following gene: 126207777

Centisome position: 14.09

GC content: 31.14

Gene sequence:

>1095_bases
ATGAACATTTTAGAGCTGATTAAAGATTGCGAAGTGGAATGGAAAAGTTTGGGGGAAGTAGCTAAATACGAACAGCCAAC
AAAATATTTGGTAAAGTCCACAAATTACAATGATGATTTTAACACTCCTGTTTTAACAGCAGGTAAAACATTTATTTTGG
GTTATACAGATGAAATTGATGGAATTTACCCAGCGAAGTCAAACCCTATAATTATTTTTGATGATTTCACTACGGCTAAT
AAATGGGTGGATTTTGATTTTAAAGTAAAATCATCAGCAATGAAAATGATCACATCAAGTGATGAAAATAAGTTCTCATT
AAAATATATTTATTATTGGTTAAATACATTGCCAATGGAAGATAACACAGATCATAAACGTCAATGGATTAGTAATTTTG
CAAATAAGAAAATCCCAATCCCCCCTCTAGAAATTCAAGAAAAAATCGTAAAAACACTTGACATTTTTACGAAGCTGGAA
GCTGAATTATCGCTGCGTGTCAAGCAATATGATTATTATCGTAATGAGTTATTAACTTTTGACGATGATGTGGAGTTTAT
AACACTGGATAAAATTTCCGAAAATTTAAACTCAATGAGAAAACCTATAAAAAGTGGTTTGCGTGAGAAGGGTCGTATTC
CATATTATGGAGCATCTGGAATTGTAGATTATGTAGAAGATTATATTTTTGATGATGAAATATTATTAATTTCAGAAGAT
GGTGCAAATTTGATTGCAAGAAATACACCTATTGCATTTAGTGTGCTTGGTAAATGTTGGGTTAATAATCATGCTCACGT
TTTAAAATTTAAAACAGATGTTGAACGTAAATTTGTTGAGTTTTATTTAAACAACTTAGATTTAAGTCCTTTTATTTCAG
GTGCAGCCCAGCCTAAGCTAAATAAACAAAATCTCAATAAAATACCTATTCCTAATATTACATTTGCCACTCAACAAAAA
ATCGTGGATATTTTAGATAAATTTGACCGCTTGCCGAACTCTATTTCAGACGGTTTACCAAAAGAAATTGAACTTAGACG
TAAGCAGTATGAGTATTATCGTGAGCGGTTGCTTAATTTTCCTAAAAGTGAGTAA

Upstream 100 bases:

>100_bases
ATTGGTTTGTGTGGGTAGGCGAATGATGGGCTAAAGTCCATCCTACAAGAGATAAAGAGCGGTCAAATTTTAACGAATTT
TTGCAAATGGAAAATAAACA

Downstream 100 bases:

>100_bases
TTAAAATGAGTAAGACTTTATTAGATATCGCCAAAGAACTTAAAAATAATGATAAGAAAGTTCAGTTGATTTATGCATTT
AATGGTACGGGTAAAACTCG

Product: putative Type I restriction enzyme EcoR124II specificity protein

Products: NA

Alternate protein names: S.EcoR124II; Type I restriction enzyme EcoR124II specificity protein; S protein [H]

Number of amino acids: Translated: 364; Mature: 364

Protein sequence:

>364_residues
MNILELIKDCEVEWKSLGEVAKYEQPTKYLVKSTNYNDDFNTPVLTAGKTFILGYTDEIDGIYPAKSNPIIIFDDFTTAN
KWVDFDFKVKSSAMKMITSSDENKFSLKYIYYWLNTLPMEDNTDHKRQWISNFANKKIPIPPLEIQEKIVKTLDIFTKLE
AELSLRVKQYDYYRNELLTFDDDVEFITLDKISENLNSMRKPIKSGLREKGRIPYYGASGIVDYVEDYIFDDEILLISED
GANLIARNTPIAFSVLGKCWVNNHAHVLKFKTDVERKFVEFYLNNLDLSPFISGAAQPKLNKQNLNKIPIPNITFATQQK
IVDILDKFDRLPNSISDGLPKEIELRRKQYEYYRERLLNFPKSE

Sequences:

>Translated_364_residues
MNILELIKDCEVEWKSLGEVAKYEQPTKYLVKSTNYNDDFNTPVLTAGKTFILGYTDEIDGIYPAKSNPIIIFDDFTTAN
KWVDFDFKVKSSAMKMITSSDENKFSLKYIYYWLNTLPMEDNTDHKRQWISNFANKKIPIPPLEIQEKIVKTLDIFTKLE
AELSLRVKQYDYYRNELLTFDDDVEFITLDKISENLNSMRKPIKSGLREKGRIPYYGASGIVDYVEDYIFDDEILLISED
GANLIARNTPIAFSVLGKCWVNNHAHVLKFKTDVERKFVEFYLNNLDLSPFISGAAQPKLNKQNLNKIPIPNITFATQQK
IVDILDKFDRLPNSISDGLPKEIELRRKQYEYYRERLLNFPKSE
>Mature_364_residues
MNILELIKDCEVEWKSLGEVAKYEQPTKYLVKSTNYNDDFNTPVLTAGKTFILGYTDEIDGIYPAKSNPIIIFDDFTTAN
KWVDFDFKVKSSAMKMITSSDENKFSLKYIYYWLNTLPMEDNTDHKRQWISNFANKKIPIPPLEIQEKIVKTLDIFTKLE
AELSLRVKQYDYYRNELLTFDDDVEFITLDKISENLNSMRKPIKSGLREKGRIPYYGASGIVDYVEDYIFDDEILLISED
GANLIARNTPIAFSVLGKCWVNNHAHVLKFKTDVERKFVEFYLNNLDLSPFISGAAQPKLNKQNLNKIPIPNITFATQQK
IVDILDKFDRLPNSISDGLPKEIELRRKQYEYYRERLLNFPKSE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 42468; Mature: 42468

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILELIKDCEVEWKSLGEVAKYEQPTKYLVKSTNYNDDFNTPVLTAGKTFILGYTDEID
CCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCC
GIYPAKSNPIIIFDDFTTANKWVDFDFKVKSSAMKMITSSDENKFSLKYIYYWLNTLPME
CCCCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHHHHCCCCCCEEEEEEEEEHHHCCCC
DNTDHKRQWISNFANKKIPIPPLEIQEKIVKTLDIFTKLEAELSLRVKQYDYYRNELLTF
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCEEE
DDDVEFITLDKISENLNSMRKPIKSGLREKGRIPYYGASGIVDYVEDYIFDDEILLISED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCCEEEEECC
GANLIARNTPIAFSVLGKCWVNNHAHVLKFKTDVERKFVEFYLNNLDLSPFISGAAQPKL
CCCEEECCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
NKQNLNKIPIPNITFATQQKIVDILDKFDRLPNSISDGLPKEIELRRKQYEYYRERLLNF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCC
PKSE
CCCC
>Mature Secondary Structure
MNILELIKDCEVEWKSLGEVAKYEQPTKYLVKSTNYNDDFNTPVLTAGKTFILGYTDEID
CCHHHHHHHCCHHHHHHHHHHHCCCCHHHHHCCCCCCCCCCCCEEECCCEEEEEECCCCC
GIYPAKSNPIIIFDDFTTANKWVDFDFKVKSSAMKMITSSDENKFSLKYIYYWLNTLPME
CCCCCCCCCEEEEECCCCCCCEEEEEEEEHHHHHHHHHCCCCCCEEEEEEEEEHHHCCCC
DNTDHKRQWISNFANKKIPIPPLEIQEKIVKTLDIFTKLEAELSLRVKQYDYYRNELLTF
CCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHCCEEE
DDDVEFITLDKISENLNSMRKPIKSGLREKGRIPYYGASGIVDYVEDYIFDDEILLISED
CCCCCEEEHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCHHHHHHHHHHCCCCEEEEECC
GANLIARNTPIAFSVLGKCWVNNHAHVLKFKTDVERKFVEFYLNNLDLSPFISGAAQPKL
CCCEEECCCCHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHCCCCCCCC
NKQNLNKIPIPNITFATQQKIVDILDKFDRLPNSISDGLPKEIELRRKQYEYYRERLLNF
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHCC
PKSE
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]