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The map label for this gene is rsmE [H]

Identifier: 126207721

GI number: 126207721

Start: 251716

End: 252447

Strand: Reverse

Name: rsmE [H]

Synonym: APL_0233

Alternate gene names: 126207721

Gene position: 252447-251716 (Counterclockwise)

Preceding gene: 126207722

Following gene: 126207720

Centisome position: 11.1

GC content: 43.99

Gene sequence:

>732_bases
ATGCGTATTCCAAGAATCTATCATCCCGAATTATTAGCAAACCGAACCGATTGTATTTTAAGTGAAGATGCGACCAATCA
CGTCGGTCGAGTATTACGAATGACGGAAGGTGAGCAAATTATTTTATTTGACGGCTCAAACCATATTTTCCACGCCACAT
TACAAGCGGTCGGGAAAAAGCAAATTATTGCAAAAATTGATGGTAGCGAATTGGATAATCGCGAATCCAATTTACCGATT
CACTTAGGGCAGGTGATTTCTCGCGGCGATCGGATGGAATTTACCATTCAAAAATCGGTTGAGCTAGGCGTAAAAACCAT
TACTCCGCTTTGGTCTGAACGTTGCGGGGTGAAACTGAACGATGAACGCCAAGACAAAAAACTACAGCAATGGCAAAAAA
TTGCGATTGCCGCTTGCGAACAATGCGGACGTAATGAGATTCCTGAAATTCGCCCGATTATGAAACTGACCGACTGGTGT
AAAGAACAAGACGATATGCTGAAATTAAATCTGCATCCTCGAGCGAAATATACTATTCGCCAGTTACCGAATGTGCCTGA
AGCCGGTGTGCGTTTATTAATCGGTTCGGAAGGCGGCTTATCCGCTGAAGAAATCGCGATGACCGAAACCCAAGGCTTTA
CCGAAGTTTTACTCGGCAAACGAGTCTTGCGTACGGAAACCGCCTCACTCGCAGCAATTACAGCCCTACAGGTTTGTTTT
GGAGATATTTAA

Upstream 100 bases:

>100_bases
AGAGATAATTCTCTTCTCAATTTAAATTGTTATCCAGCGTTTATTTCACAAGTCATTTTCATAATAATAGTCACAATGAT
TGTTAATTAAAGAACATCTT

Downstream 100 bases:

>100_bases
TGTTAGAAAATTTACAAGGCAAATTTTTGATTGCAACGCCGGAAATTGATGACGATTACTTTGATCGCACTGTGATTTAT
ATTTGCGAACATAACAGTAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 243; Mature: 243

Protein sequence:

>243_residues
MRIPRIYHPELLANRTDCILSEDATNHVGRVLRMTEGEQIILFDGSNHIFHATLQAVGKKQIIAKIDGSELDNRESNLPI
HLGQVISRGDRMEFTIQKSVELGVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
KEQDDMLKLNLHPRAKYTIRQLPNVPEAGVRLLIGSEGGLSAEEIAMTETQGFTEVLLGKRVLRTETASLAAITALQVCF
GDI

Sequences:

>Translated_243_residues
MRIPRIYHPELLANRTDCILSEDATNHVGRVLRMTEGEQIILFDGSNHIFHATLQAVGKKQIIAKIDGSELDNRESNLPI
HLGQVISRGDRMEFTIQKSVELGVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
KEQDDMLKLNLHPRAKYTIRQLPNVPEAGVRLLIGSEGGLSAEEIAMTETQGFTEVLLGKRVLRTETASLAAITALQVCF
GDI
>Mature_243_residues
MRIPRIYHPELLANRTDCILSEDATNHVGRVLRMTEGEQIILFDGSNHIFHATLQAVGKKQIIAKIDGSELDNRESNLPI
HLGQVISRGDRMEFTIQKSVELGVKTITPLWSERCGVKLNDERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWC
KEQDDMLKLNLHPRAKYTIRQLPNVPEAGVRLLIGSEGGLSAEEIAMTETQGFTEVLLGKRVLRTETASLAAITALQVCF
GDI

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=243, Percent_Identity=65.843621399177, Blast_Score=325, Evalue=2e-90,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015947
- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 27334; Mature: 27334

Theoretical pI: Translated: 6.78; Mature: 6.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPRIYHPELLANRTDCILSEDATNHVGRVLRMTEGEQIILFDGSNHIFHATLQAVGKK
CCCCCCCCHHHHCCCCCEEECCCHHHHHHHHHEECCCCEEEEEECCCCEEHHHHHHCCHH
QIIAKIDGSELDNRESNLPIHLGQVISRGDRMEFTIQKSVELGVKTITPLWSERCGVKLN
HEEEEECCCCCCCCCCCCCEEHHHHHCCCCCEEEEEEHHHHHCHHHHCHHHHHHCCCEEC
DERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWCKEQDDMLKLNLHPRAKYTIR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
QLPNVPEAGVRLLIGSEGGLSAEEIAMTETQGFTEVLLGKRVLRTETASLAAITALQVCF
HCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDI
CCC
>Mature Secondary Structure
MRIPRIYHPELLANRTDCILSEDATNHVGRVLRMTEGEQIILFDGSNHIFHATLQAVGKK
CCCCCCCCHHHHCCCCCEEECCCHHHHHHHHHEECCCCEEEEEECCCCEEHHHHHHCCHH
QIIAKIDGSELDNRESNLPIHLGQVISRGDRMEFTIQKSVELGVKTITPLWSERCGVKLN
HEEEEECCCCCCCCCCCCCEEHHHHHCCCCCEEEEEEHHHHHCHHHHCHHHHHHCCCEEC
DERQDKKLQQWQKIAIAACEQCGRNEIPEIRPIMKLTDWCKEQDDMLKLNLHPRAKYTIR
CCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHH
QLPNVPEAGVRLLIGSEGGLSAEEIAMTETQGFTEVLLGKRVLRTETASLAAITALQVCF
HCCCCCCCCEEEEEECCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDI
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]