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The map label for this gene is artJ [H]

Identifier: 126175589

GI number: 126175589

Start: 3972553

End: 3973287

Strand: Direct

Name: artJ [H]

Synonym: Sbal_3392

Alternate gene names: 126175589

Gene position: 3972553-3973287 (Clockwise)

Preceding gene: 126175587

Following gene: 126175590

Centisome position: 77.48

GC content: 44.08

Gene sequence:

>735_bases
ATGAAAAAATCCATGTTATTAGGCGGCCTGACCTTAACTGCCACCTTGTTGTTAGCTGGATGCGGAAAAAGCGATGACGT
ATTAGTCGTTGGCACCAATGCCGCCTTCCCTCCTTTTGAATATGTTGGCGGCCAAAGTGGTGATGAAATCAAAGGCTTTG
ATATCGATCTGGCCAAGCAAATCGCTAAAGATGCCGGCAAGACGCTCAAAGTCGAAAACATGAAATTCGATTCACTGATC
GTCGCACTCAACTCAGGCAAGATTGATTTTATCGCCTCAGGCATGACGATTACCGCTGAACGTCAGGCAAACGTAAACTT
CTCAGAGCCTTACTACGAAGCGACCCAAGTGGTACTGGTTAACAAAGATAACGATAGCATTCATACGCTTGAAGATATTA
AAGGCAAACACTTTGCGGTGCAGTTAGGCACAACCGCTGACATCATGTCGAAAAAATACACCCAAAATATCACCGCCTTT
AATACGGGTTTCGAAGCCATTATGGAGCTTAAAAATTCAAAGGTAGATTTAGTGTTATTCGACAGCGAACCTGCCGCTAA
TTATCTGGCTAAAAATCCTGAATTAAAGATGATCAAACTGGATTTCCCTGCTGAGTTTTATGGCTTCGCCGTGTCAAAAG
AGCAGCCTGAACTGTTGGCGAGCATCAATAAAACACTGGCTAACATGAAAGCCGACGGCCAATATCAGACGCTCGTTTCT
GCCCATATAAAGTGA

Upstream 100 bases:

>100_bases
AAATGAATTTTTAATCAAATTAACAGCATAATTATTGACACAACATGCTCATCTGCCTATAGTCGCCACACTGATAGTGA
AATCGACAAGAGAGTCTTAA

Downstream 100 bases:

>100_bases
TATCAGCATGATAGACGCAATTAACAGCTCGCTGTTCAGCCCAATTGGCGAAGACGGCATGATAGGTATACTGCTGATAT
TAAATGGTTTGAAGGTGACG

Product: extracellular solute-binding protein

Products: ADP; phosphate; L-arginine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 244

Protein sequence:

>244_residues
MKKSMLLGGLTLTATLLLAGCGKSDDVLVVGTNAAFPPFEYVGGQSGDEIKGFDIDLAKQIAKDAGKTLKVENMKFDSLI
VALNSGKIDFIASGMTITAERQANVNFSEPYYEATQVVLVNKDNDSIHTLEDIKGKHFAVQLGTTADIMSKKYTQNITAF
NTGFEAIMELKNSKVDLVLFDSEPAANYLAKNPELKMIKLDFPAEFYGFAVSKEQPELLASINKTLANMKADGQYQTLVS
AHIK

Sequences:

>Translated_244_residues
MKKSMLLGGLTLTATLLLAGCGKSDDVLVVGTNAAFPPFEYVGGQSGDEIKGFDIDLAKQIAKDAGKTLKVENMKFDSLI
VALNSGKIDFIASGMTITAERQANVNFSEPYYEATQVVLVNKDNDSIHTLEDIKGKHFAVQLGTTADIMSKKYTQNITAF
NTGFEAIMELKNSKVDLVLFDSEPAANYLAKNPELKMIKLDFPAEFYGFAVSKEQPELLASINKTLANMKADGQYQTLVS
AHIK
>Mature_244_residues
MKKSMLLGGLTLTATLLLAGCGKSDDVLVVGTNAAFPPFEYVGGQSGDEIKGFDIDLAKQIAKDAGKTLKVENMKFDSLI
VALNSGKIDFIASGMTITAERQANVNFSEPYYEATQVVLVNKDNDSIHTLEDIKGKHFAVQLGTTADIMSKKYTQNITAF
NTGFEAIMELKNSKVDLVLFDSEPAANYLAKNPELKMIKLDFPAEFYGFAVSKEQPELLASINKTLANMKADGQYQTLVS
AHIK

Specific function: Part of the ABC transporter complex ArtPIQMJ involved in arginine transport. Binds L-arginine with high affinity [H]

COG id: COG0834

COG function: function code ET; ABC-type amino acid transport/signal transduction systems, periplasmic component/domain

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 3 family [H]

Homologues:

Organism=Escherichia coli, GI1787085, Length=219, Percent_Identity=37.8995433789954, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1787088, Length=248, Percent_Identity=30.6451612903226, Blast_Score=113, Evalue=1e-26,
Organism=Escherichia coli, GI1787031, Length=220, Percent_Identity=33.1818181818182, Blast_Score=111, Evalue=3e-26,
Organism=Escherichia coli, GI1788228, Length=220, Percent_Identity=29.5454545454545, Blast_Score=103, Evalue=7e-24,
Organism=Escherichia coli, GI1788648, Length=227, Percent_Identity=29.9559471365639, Blast_Score=98, Evalue=6e-22,
Organism=Escherichia coli, GI1788649, Length=255, Percent_Identity=26.2745098039216, Blast_Score=83, Evalue=2e-17,

Paralogues:

None

Copy number: 1180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015683
- InterPro:   IPR005768
- InterPro:   IPR001638
- InterPro:   IPR018313 [H]

Pfam domain/function: PF00497 SBP_bac_3 [H]

EC number: NA

Molecular weight: Translated: 26547; Mature: 26547

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS01039 SBP_BACTERIAL_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSMLLGGLTLTATLLLAGCGKSDDVLVVGTNAAFPPFEYVGGQSGDEIKGFDIDLAKQ
CCCCEEECCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHH
IAKDAGKTLKVENMKFDSLIVALNSGKIDFIASGMTITAERQANVNFSEPYYEATQVVLV
HHHHCCCEEEEECEEECEEEEEEECCCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEE
NKDNDSIHTLEDIKGKHFAVQLGTTADIMSKKYTQNITAFNTGFEAIMELKNSKVDLVLF
ECCCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHCCCEEECHHHHHHHHHCCCCEEEEEE
DSEPAANYLAKNPELKMIKLDFPAEFYGFAVSKEQPELLASINKTLANMKADGQYQTLVS
CCCCCHHHHHCCCCEEEEEEECCHHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEE
AHIK
ECCC
>Mature Secondary Structure
MKKSMLLGGLTLTATLLLAGCGKSDDVLVVGTNAAFPPFEYVGGQSGDEIKGFDIDLAKQ
CCCCEEECCHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHCCCCCCCCCCCCCHHHHHH
IAKDAGKTLKVENMKFDSLIVALNSGKIDFIASGMTITAERQANVNFSEPYYEATQVVLV
HHHHCCCEEEEECEEECEEEEEEECCCEEEEECCEEEEEECCCCCCCCCCCCCEEEEEEE
NKDNDSIHTLEDIKGKHFAVQLGTTADIMSKKYTQNITAFNTGFEAIMELKNSKVDLVLF
ECCCCCEEEHHHCCCCEEEEEECCHHHHHHHHHHCCCEEECHHHHHHHHHCCCCEEEEEE
DSEPAANYLAKNPELKMIKLDFPAEFYGFAVSKEQPELLASINKTLANMKADGQYQTLVS
CCCCCHHHHHCCCCEEEEEEECCHHHHEEEECCCCHHHHHHHHHHHHHCCCCCCCEEEEE
AHIK
ECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-arginine [Periplasm]; H2O [C]

Specific reaction: ATP + L-arginine [Periplasm] + H2O = ADP + phosphate + L-arginine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8501075; 8905232; 9278503; 9298646; 9600841 [H]