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The map label for this gene is truD [H]

Identifier: 126175324

GI number: 126175324

Start: 3658418

End: 3659551

Strand: Reverse

Name: truD [H]

Synonym: Sbal_3123

Alternate gene names: 126175324

Gene position: 3659551-3658418 (Counterclockwise)

Preceding gene: 126175325

Following gene: 126175323

Centisome position: 71.37

GC content: 47.18

Gene sequence:

>1134_bases
ATGAGCGAATTACATTATCTGTATGGCAAGCCTTTAGGTACAGCAGATCTAAGAACCCAAAATAGCGATTTTATCGTGAA
AGAGATTTTGCCTTTTAGCCCAACGGGTGAAGGTGAACATCACATGCTGCATGTACGCAAAGAAGGGCTAAATACAGTAC
AAGTCGCTGAAATGATCGCTAAGTTCGCTAAGGTGCATCCAAAGGAAGTCACCTACGCTGGGCAAAAAGATAAAAATGCC
ATCACAGAACAATGGTTCGGTGTGCGTATTCCGGGTAAAGAAACGCCGCTTTGGAAGGATCTCAATAGCGAGCGTTTGAC
TATATTGTCGAGCAGCCGCCACAGCAAAAAACTGAGAATCGGTGCTTTAGTCGGTAACCGTTTTATTCTTACCCTGAGAA
ATGTCACCCAAGTCGAAGACATTATCAGCCGTATCGAAAAGGTCGCTCAAGTGGGCGTGCCCAATTACTTTGGTGAGCAA
CGCTTTGGTCACGATGGTAAAAACTTAGTTTTTGGTCGCCAAATGATGGCGGGTAAAAAAGTCAAAGATAGAAATAAGCG
CAGCATGTATTTGTCTGCGGTGCGCTCAAATCTGTTTAACACTGTGGTGTCGTACCGTTTAGCCAACCATGGCACTAAGC
CGCTAGCGGGTGATTGTGTGATGTTGGCGGGCAGCAAAAGCTTCTTCGTTACGCCTGAATGGGATCTCATCGTATTGAAA
CGTCTGATTGAAAAAGACATTCAACTATCAGCACCCTTATGGGGCCGTGGCATGATGCTGCCTCAAGGTGAAGCTGCTGC
GGTGGAAACCTTGGCCATGAGTGAGTTGGCCGACGATTGCTATGGCCTTGAACATGCGGGATTAGAGCAAGAGCGTCGTC
CGTTACTGCTTGAGCCCCAAGCCCTTAAATACGAACAAACCGCCGATGGTCTTGTGCTGGAGTTTGTGTTGCCTGCGGGC
AGTTTTGCCACATCCTTGTTGAGAGAGTTAGTCAATTATCAAGATGTTAAAGAGCAGCAATGGCAAGCGACACTGGTGCA
GGAGCCTGCACCCGAGGCAACGAATGCTAATGTTGCGGCTGATACAATTGAAACTAAGACTGAAGCTGCGCCTAACATTG
AAGCAGAGTCATGA

Upstream 100 bases:

>100_bases
CTGAACAGCTAGGATTTACCGGGCGTAAAGAAGGTATTGCGGTAGAAGCCGTGGTATTGATGACCCGTAAACACGATTAA
CTTAAGAGTAAAAAAGTCCC

Downstream 100 bases:

>100_bases
TCCGCATCTTAGTGAGCAACGATGACGGGGTTAATGCACCGGGGATCAAAGCCTTAACTGAGGCCTTGACCGAAATTGCG
ACTGTGCTGACAGTGGGGCC

Product: tRNA pseudouridine synthase D TruD

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHMLHVRKEGLNTVQVAEMIAKFAKVHPKEVTYAGQKDKNA
ITEQWFGVRIPGKETPLWKDLNSERLTILSSSRHSKKLRIGALVGNRFILTLRNVTQVEDIISRIEKVAQVGVPNYFGEQ
RFGHDGKNLVFGRQMMAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLIVLK
RLIEKDIQLSAPLWGRGMMLPQGEAAAVETLAMSELADDCYGLEHAGLEQERRPLLLEPQALKYEQTADGLVLEFVLPAG
SFATSLLRELVNYQDVKEQQWQATLVQEPAPEATNANVAADTIETKTEAAPNIEAES

Sequences:

>Translated_377_residues
MSELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHMLHVRKEGLNTVQVAEMIAKFAKVHPKEVTYAGQKDKNA
ITEQWFGVRIPGKETPLWKDLNSERLTILSSSRHSKKLRIGALVGNRFILTLRNVTQVEDIISRIEKVAQVGVPNYFGEQ
RFGHDGKNLVFGRQMMAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLIVLK
RLIEKDIQLSAPLWGRGMMLPQGEAAAVETLAMSELADDCYGLEHAGLEQERRPLLLEPQALKYEQTADGLVLEFVLPAG
SFATSLLRELVNYQDVKEQQWQATLVQEPAPEATNANVAADTIETKTEAAPNIEAES
>Mature_376_residues
SELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHMLHVRKEGLNTVQVAEMIAKFAKVHPKEVTYAGQKDKNAI
TEQWFGVRIPGKETPLWKDLNSERLTILSSSRHSKKLRIGALVGNRFILTLRNVTQVEDIISRIEKVAQVGVPNYFGEQR
FGHDGKNLVFGRQMMAGKKVKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLIVLKR
LIEKDIQLSAPLWGRGMMLPQGEAAAVETLAMSELADDCYGLEHAGLEQERRPLLLEPQALKYEQTADGLVLEFVLPAGS
FATSLLRELVNYQDVKEQQWQATLVQEPAPEATNANVAADTIETKTEAAPNIEAES

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=145, Percent_Identity=33.7931034482759, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI148762961, Length=145, Percent_Identity=33.7931034482759, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI148762948, Length=145, Percent_Identity=33.7931034482759, Blast_Score=72, Evalue=8e-13,
Organism=Homo sapiens, GI50727002, Length=133, Percent_Identity=32.3308270676692, Blast_Score=67, Evalue=2e-11,
Organism=Escherichia coli, GI1789102, Length=336, Percent_Identity=44.6428571428571, Blast_Score=262, Evalue=3e-71,
Organism=Drosophila melanogaster, GI21355799, Length=122, Percent_Identity=34.4262295081967, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 42086; Mature: 41955

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHMLHVRKEGLNTVQVAEMIA
CCCCCCHHCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
KFAKVHPKEVTYAGQKDKNAITEQWFGVRIPGKETPLWKDLNSERLTILSSSRHSKKLRI
HHHHCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHCCCCCEEEEEECCCCCCEEEE
GALVGNRFILTLRNVTQVEDIISRIEKVAQVGVPNYFGEQRFGHDGKNLVFGRQMMAGKK
EEEECCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEHHHHHCCCC
VKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLIVLK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCCHHHHHH
RLIEKDIQLSAPLWGRGMMLPQGEAAAVETLAMSELADDCYGLEHAGLEQERRPLLLEPQ
HHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCH
ALKYEQTADGLVLEFVLPAGSFATSLLRELVNYQDVKEQQWQATLVQEPAPEATNANVAA
HHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCH
DTIETKTEAAPNIEAES
HHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SELHYLYGKPLGTADLRTQNSDFIVKEILPFSPTGEGEHHMLHVRKEGLNTVQVAEMIA
CCCCCHHCCCCCCCCCCCCCCCEEHHHHCCCCCCCCCCCEEEEEECCCCCHHHHHHHHH
KFAKVHPKEVTYAGQKDKNAITEQWFGVRIPGKETPLWKDLNSERLTILSSSRHSKKLRI
HHHHCCCHHEEECCCCCCHHHHHHHCEEECCCCCCCCHHCCCCCEEEEEECCCCCCEEEE
GALVGNRFILTLRNVTQVEDIISRIEKVAQVGVPNYFGEQRFGHDGKNLVFGRQMMAGKK
EEEECCEEEEEECHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCEEEHHHHHCCCC
VKDRNKRSMYLSAVRSNLFNTVVSYRLANHGTKPLAGDCVMLAGSKSFFVTPEWDLIVLK
HHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCEEEECCCCHHHHHH
RLIEKDIQLSAPLWGRGMMLPQGEAAAVETLAMSELADDCYGLEHAGLEQERRPLLLEPQ
HHHHHHHEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCEEECCH
ALKYEQTADGLVLEFVLPAGSFATSLLRELVNYQDVKEQQWQATLVQEPAPEATNANVAA
HHCCCCCCCCEEEEEEECCCHHHHHHHHHHHCHHHHHHHHHHHHHHCCCCCCCCCCCCCH
DTIETKTEAAPNIEAES
HHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12368813 [H]