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The map label for this gene is insI1 [C]

Identifier: 126174370

GI number: 126174370

Start: 2497321

End: 2498262

Strand: Direct

Name: insI1 [C]

Synonym: Sbal_2153

Alternate gene names: 126174370

Gene position: 2497321-2498262 (Clockwise)

Preceding gene: 126174368

Following gene: 126174372

Centisome position: 48.71

GC content: 45.97

Gene sequence:

>942_bases
ATGAATTATCAACAGTTGACCGAAGCAAGACGATACCAGATTTCCGTGCTGTTAGAACAGGGCTTTTCGATAGCACACAT
AGCTAAATGCATTGATTGTCATCGCTCTAGTGTCTATAGGGAAGTTAAACGATGCCAAGGGCAATCACGCTATCAGCCTG
ATATTGCACACAAGCAGGCTGTCACAATGAGGCGTCAGTCGAGTAAATATGCGATACCCTCACAGCGAATAGAGAGCATC
ATCCTTTTGCTGCAATTGGATTGGAGCCCGGAGCAAATTTCGCAGGTGTTGCGTCTGGTGAAGGAGCCCGTCAGTCACGA
GTGGATTTATCGCTATATTGCACGCGACAAACGCCGTGGTGGCAAACTTTATCGTCATTTACGTCAAGGACATAAGCGTT
ATCGGCGCGGTAAAGTAGAGCAAGCTCCTACCATAAAAAACGCCGTATCCATTGACGACAGACCAGCCATTGTTGATAGC
CGAGAGCGATTTGGTGACTGGGAAATCGACACCGTATTGGGGCTGCATGGAACGGGTTCGATTGTAACCCTATTAGAGCG
TAAGACTCGGTTTTATTTGATAAAGAAAGTCAATTCGAAGTCAGCGGCAGATGTAACGAAAGCGACGATTGAGTTACTGA
TGCCCTATAAGGCGCATGTGCTGACAATCACGGCAGATAATGGACGCGAATTTGCTCACCACCAAGAGATAGCAGAGGCG
CTAGATACACAGGTGTATTTTGCGCACCCTTATCGTTCTTGTGAACGAGGAGCGAATGAAAACGCCAATGGATTACTGCG
GCAGTACGTCAAAAAAGGGGTTGATTTGAGGCTAGTGAGTGATGAATTAATCGAATTTGCTCAGAATAGAATTAACTATC
GACCCAAAAAGTGTTTGAAGTTTAAGCAGCCTGCGGTGGTATTTCACCAATTAGCTGCTTAA

Upstream 100 bases:

>100_bases
AGAATTCAGATAATAACTGCGACACTCTAATCGAGGTGTAGAAGAAGCCAACTGCTGAAAAGTGGTACGCTTTATCTCGC
CAAAGTAAAAGAGTATCACC

Downstream 100 bases:

>100_bases
TTGTCGAGTGTCGCACTTCGGAGTTGAATTCGGGAGCTACCTAAAAGCTATGCTAGTAATGAAACTGGAGAGGTGAACTC
TAAATTCAGGGCGTGAGCCA

Product: integrase catalytic subunit

Products: NA

Alternate protein names: Transposon TN4551 [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNYQQLTEARRYQISVLLEQGFSIAHIAKCIDCHRSSVYREVKRCQGQSRYQPDIAHKQAVTMRRQSSKYAIPSQRIESI
ILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGKLYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDS
RERFGDWEIDTVLGLHGTGSIVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEA
LDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFKQPAVVFHQLAA

Sequences:

>Translated_313_residues
MNYQQLTEARRYQISVLLEQGFSIAHIAKCIDCHRSSVYREVKRCQGQSRYQPDIAHKQAVTMRRQSSKYAIPSQRIESI
ILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGKLYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDS
RERFGDWEIDTVLGLHGTGSIVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEA
LDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFKQPAVVFHQLAA
>Mature_313_residues
MNYQQLTEARRYQISVLLEQGFSIAHIAKCIDCHRSSVYREVKRCQGQSRYQPDIAHKQAVTMRRQSSKYAIPSQRIESI
ILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRGGKLYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDS
RERFGDWEIDTVLGLHGTGSIVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEA
LDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLKFKQPAVVFHQLAA

Specific function: Required for the transposition of the insertion element (Potential) [H]

COG id: COG2826

COG function: function code L; Transposase and inactivated derivatives, IS30 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

Organism=Escherichia coli, GI1786450, Length=327, Percent_Identity=30.2752293577982, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1790736, Length=327, Percent_Identity=30.2752293577982, Blast_Score=133, Evalue=1e-32,
Organism=Escherichia coli, GI1787671, Length=327, Percent_Identity=30.2752293577982, Blast_Score=133, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR001584
- InterPro:   IPR012337
- InterPro:   IPR001598 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 36355; Mature: 36355

Theoretical pI: Translated: 10.27; Mature: 10.27

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYQQLTEARRYQISVLLEQGFSIAHIAKCIDCHRSSVYREVKRCQGQSRYQPDIAHKQA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VTMRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRG
HHHHHHCCCCCCCHHHHHHEEEHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GKLYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHCCCCCHHHEEECCCCCH
IVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEA
HHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
LDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLK
HCCCEEEECCHHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHH
FKQPAVVFHQLAA
CCCHHHHHHHHCC
>Mature Secondary Structure
MNYQQLTEARRYQISVLLEQGFSIAHIAKCIDCHRSSVYREVKRCQGQSRYQPDIAHKQA
CCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
VTMRRQSSKYAIPSQRIESIILLLQLDWSPEQISQVLRLVKEPVSHEWIYRYIARDKRRG
HHHHHHCCCCCCCHHHHHHEEEHEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
GKLYRHLRQGHKRYRRGKVEQAPTIKNAVSIDDRPAIVDSRERFGDWEIDTVLGLHGTGS
HHHHHHHHHHHHHHHCCCCCCCCCHHHHCCCCCCCCCCCCHHHCCCCCHHHEEECCCCCH
IVTLLERKTRFYLIKKVNSKSAADVTKATIELLMPYKAHVLTITADNGREFAHHQEIAEA
HHHHHHHHHHEEEEEECCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHH
LDTQVYFAHPYRSCERGANENANGLLRQYVKKGVDLRLVSDELIEFAQNRINYRPKKCLK
HCCCEEEECCHHHHHCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHHHHCCCCHHHHHH
FKQPAVVFHQLAA
CCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2820936 [H]