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The map label for this gene is thrS

Identifier: 126174365

GI number: 126174365

Start: 2492548

End: 2494476

Strand: Direct

Name: thrS

Synonym: Sbal_2148

Alternate gene names: 126174365

Gene position: 2492548-2494476 (Clockwise)

Preceding gene: 126174362

Following gene: 126174366

Centisome position: 48.61

GC content: 46.24

Gene sequence:

>1929_bases
ATGCCTGTAATTACACTTCCCGATGGTAGCAAACGCGAGTTTGCTCATGCTGTATCGACCCTCGATGTTGCCGCCGATAT
TGGCCCAGGTTTAGCGAAAGCTTGTATCGCTGGCCGTGTTAATGGCGAATTAAAAGATGCTTGCGATCTCATTGAAACCG
ATGCAGAGCTATCAATCATTACCGCCAAAGACGAAGAAGGTGTTGAAATTCTTCGCCATTCTTGCGCACATTTATTAGGT
CACGCGATTAAGCAAATGTGGCCAGAAACCAAGATGGCGATTGGTCCAGTTATCGATAACGGCTTCTACTATGATATCGA
CCTTGAGCACAAGTTGACTCAAGATGACATCGACGCCTTAGAAAAGCGCATGCTTCAACTGGCAAAAACTAACTATGACG
TTGTAAAACGTGTGGTTAGCTGGCAAGAAGCGCGCGATACATTCGCTGCGCGCGGCGAAGATTATAAGATTGCCATTCTG
GATGAGAACATCAGCAAAGACGCGACGCCTGCGCTGTATCATCACGAAGAATACACAGACATGTGTCGTGGACCGCACGT
GCCTAACATGCGTTTCTGCCAACATTTCAAATTAATGAGCATTGCTGGCGCTTACTGGCGTGGCAACTCAGAAAACAAGA
TGTTGCAACGTATCTATGGTACTGCTTGGGCTGACAAAAAAGCCTTGAGCACACACTTAACCCGTCTTGAAGAAGCGGCT
AAGCGTGACCACCGTAAAATCGGTAAGCAGCTCGACTTGTATCACATGCAAGAAGAAGCACCGGGTATGGTGTTCTGGCA
CAACGACGGTTGGAGCATCTTCCTCGAATTAGAAAGATTCATTCGCCGTAAGTTAAACCAATACACTTACCAAGAAGTGA
AAGGCCCATTAATGATGGACCGCGTGTTGTGGGAACGTTCTGGTCACTGGGATAAATACTCAGAAGCCATGTTCACGACC
AGCAGTGAAAACCGCGAATATGCTATCAAGCCAATGAACTGCCCAGGCCACGTGCAGATCTTCAACCAAGGGTTGAAATC
TTACCGCGACCTGCCATTACGTATGGCTGAGTTTGGTTGCTGTCACCGTAATGAACCTTCTGGTTCGTTACACGGATTAA
TGCGCGTACGTGGCTTTACCCAAGACGACGCCCATATTTTCTGTACTGACAGCCAAGTTCAAGAGGAAGTGAGTGCTTGT
ATCCAGATGGTTTACGATACATACGCCACCTTCGGCTTTGAAAATATCGTGGTAAAACTGTCGACTCGCCCAGAAAAACG
TATCGGTGACGATGCGATGTGGGACAGAGCGGAAGAAGCGCTTAAGCAAGCTCTGCGTGACAATAATATTGAATTCACCA
TTTTGCCAGGTGAAGGCGCGTTCTACGGACCAAAAATCGAATTTACTTTACACGATTGTTTGGACCGTGCGTGGCAATGT
GGTACTGTGCAGCTCGATTATGCGTTGCCAAGCCGTCTTGGTGCAACTTACGTTGCCGAAGATAATAGCCGTCAGACCCC
AGTGATGATCCATCGTGCCATTTTAGGCTCTTTGGAACGATTCTTGGGTATCTTGATAGAAGAATATGCAGGACGTTTCC
CAACTTGGTTAGCCCCAATGCAAGTTGTCGTGATGAATATCACTGATAAACAGGCTGATTATGTTGAAGAAGTCGTAAAA
TTCTTCAAAGAACAAGGTATTCGAGCGTCTTTTGACTTGAGGAATGAGAAAATAGGCTTTAAAATACGCGAACACACCTT
AAGGCGTGTCCCTTATTTGTTGGTCGTTGGCGATCAAGAAATGGAAAATAAGGAAGTAGCGGTGCGTACCCGTGATGGTA
TCGATTTAGGTAAGATGCGACTTGAAGATTTCGCCACTAAAATCCATCAACAGATTTCGCTCCGTAGTCTCAAATTGTTG
GAGGAATAG

Upstream 100 bases:

>100_bases
GGCTAAGACTCCTGTACTGTCTTAACCTAGCGAGCATTTCTCTTACATTAACTAAAACCAAGTGGCCCTACGTGTGGGTC
ACCACTGGAAAGGACATTTC

Downstream 100 bases:

>100_bases
GTCATAAAGATCAAAAAAACAGCAGGGCGTCAGCCGGCCCCTAATAGAATCAATGAAGAAATCACAGGTGTACCTGAAGT
ACGCTTAACTGGCATTGATG

Product: threonyl-tRNA synthetase

Products: NA

Alternate protein names: Threonine--tRNA ligase; ThrRS

Number of amino acids: Translated: 642; Mature: 641

Protein sequence:

>642_residues
MPVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSIITAKDEEGVEILRHSCAHLLG
HAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIDALEKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEDYKIAIL
DENISKDATPALYHHEEYTDMCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMDRVLWERSGHWDKYSEAMFTT
SSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCTDSQVQEEVSAC
IQMVYDTYATFGFENIVVKLSTRPEKRIGDDAMWDRAEEALKQALRDNNIEFTILPGEGAFYGPKIEFTLHDCLDRAWQC
GTVQLDYALPSRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPMQVVVMNITDKQADYVEEVVK
FFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQEMENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLL
EE

Sequences:

>Translated_642_residues
MPVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSIITAKDEEGVEILRHSCAHLLG
HAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIDALEKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEDYKIAIL
DENISKDATPALYHHEEYTDMCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMDRVLWERSGHWDKYSEAMFTT
SSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCTDSQVQEEVSAC
IQMVYDTYATFGFENIVVKLSTRPEKRIGDDAMWDRAEEALKQALRDNNIEFTILPGEGAFYGPKIEFTLHDCLDRAWQC
GTVQLDYALPSRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPMQVVVMNITDKQADYVEEVVK
FFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQEMENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLL
EE
>Mature_641_residues
PVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSIITAKDEEGVEILRHSCAHLLGH
AIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIDALEKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEDYKIAILD
ENISKDATPALYHHEEYTDMCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAAK
RDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMDRVLWERSGHWDKYSEAMFTTS
SENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGCCHRNEPSGSLHGLMRVRGFTQDDAHIFCTDSQVQEEVSACI
QMVYDTYATFGFENIVVKLSTRPEKRIGDDAMWDRAEEALKQALRDNNIEFTILPGEGAFYGPKIEFTLHDCLDRAWQCG
TVQLDYALPSRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPMQVVVMNITDKQADYVEEVVKF
FKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQEMENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLLE
E

Specific function: Thrs Is Also A Translational Repressor Protein, It Controls The Translation Of Its Own Gene By Binding To Its mRNA. [C]

COG id: COG0441

COG function: function code J; Threonyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI38570111, Length=618, Percent_Identity=39.9676375404531, Blast_Score=456, Evalue=1e-128,
Organism=Homo sapiens, GI38202255, Length=643, Percent_Identity=37.6360808709176, Blast_Score=438, Evalue=1e-123,
Organism=Homo sapiens, GI20070344, Length=646, Percent_Identity=35.2941176470588, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1788013, Length=642, Percent_Identity=77.4143302180685, Blast_Score=1075, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71984184, Length=629, Percent_Identity=37.8378378378378, Blast_Score=447, Evalue=1e-125,
Organism=Caenorhabditis elegans, GI71984192, Length=631, Percent_Identity=37.7179080824089, Blast_Score=446, Evalue=1e-125,
Organism=Saccharomyces cerevisiae, GI6322112, Length=628, Percent_Identity=37.7388535031847, Blast_Score=433, Evalue=1e-122,
Organism=Saccharomyces cerevisiae, GI6322654, Length=448, Percent_Identity=36.3839285714286, Blast_Score=300, Evalue=3e-82,
Organism=Drosophila melanogaster, GI24583839, Length=630, Percent_Identity=38.5714285714286, Blast_Score=464, Evalue=1e-130,
Organism=Drosophila melanogaster, GI24583842, Length=630, Percent_Identity=38.5714285714286, Blast_Score=464, Evalue=1e-130,
Organism=Drosophila melanogaster, GI28574141, Length=630, Percent_Identity=38.5714285714286, Blast_Score=464, Evalue=1e-130,

Paralogues:

None

Copy number: 700 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYT_SHEB2 (B8EES6)

Other databases:

- EMBL:   CP001252
- RefSeq:   YP_002358107.1
- ProteinModelPortal:   B8EES6
- SMR:   B8EES6
- GeneID:   7085990
- GenomeReviews:   CP001252_GR
- KEGG:   sbp:Sbal223_2184
- HOGENOM:   HBG352811
- OMA:   GFEYEVE
- ProtClustDB:   PRK00413
- GO:   GO:0005737
- HAMAP:   MF_00184_B
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR012675
- InterPro:   IPR004095
- InterPro:   IPR012676
- InterPro:   IPR002320
- InterPro:   IPR018163
- InterPro:   IPR012947
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.10.20.30
- PRINTS:   PR01047
- SMART:   SM00863
- TIGRFAMs:   TIGR00418

Pfam domain/function: PF03129 HGTP_anticodon; PF02824 TGS; PF00587 tRNA-synt_2b; PF07973 tRNA_SAD; SSF52954 Anticodon_bd; SSF81271 TGS-like; SSF55186 Thr/Ala-tRNA-synth_IIc_edit

EC number: =6.1.1.3

Molecular weight: Translated: 73747; Mature: 73616

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSII
CCEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEE
TAKDEEGVEILRHSCAHLLGHAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIDAL
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEECCEECCCEEEEEECCCCCCHHHHHHH
EKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEDYKIAILDENISKDATPALYHHEEYTD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCHHHHH
MCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMD
HHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
RVLWERSGHWDKYSEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGC
HHHHHCCCCCCHHHHHHEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTDSQVQEEVSACIQMVYDTYATFGFENIVVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
STRPEKRIGDDAMWDRAEEALKQALRDNNIEFTILPGEGAFYGPKIEFTLHDCLDRAWQC
CCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCEEEEEHHHHHHHHHCC
GTVQLDYALPSRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPM
CCEEECCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
QVVVMNITDKQADYVEEVVKFFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCC
MENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLLEE
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PVITLPDGSKREFAHAVSTLDVAADIGPGLAKACIAGRVNGELKDACDLIETDAELSII
CEEECCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCHHHHHHHHCCCCCEEEE
TAKDEEGVEILRHSCAHLLGHAIKQMWPETKMAIGPVIDNGFYYDIDLEHKLTQDDIDAL
EECCCCHHHHHHHHHHHHHHHHHHHHCCHHHHEECCEECCCEEEEEECCCCCCHHHHHHH
EKRMLQLAKTNYDVVKRVVSWQEARDTFAARGEDYKIAILDENISKDATPALYHHEEYTD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHCCHHHHH
MCRGPHVPNMRFCQHFKLMSIAGAYWRGNSENKMLQRIYGTAWADKKALSTHLTRLEEAA
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
KRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWSIFLELERFIRRKLNQYTYQEVKGPLMMD
HHHHHHHCCHHHHHHHHCCCCCEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHCCCHHHH
RVLWERSGHWDKYSEAMFTTSSENREYAIKPMNCPGHVQIFNQGLKSYRDLPLRMAEFGC
HHHHHCCCCCCHHHHHHEECCCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCHHHHHHCC
CHRNEPSGSLHGLMRVRGFTQDDAHIFCTDSQVQEEVSACIQMVYDTYATFGFENIVVKL
CCCCCCCHHHHHHHHHCCCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEE
STRPEKRIGDDAMWDRAEEALKQALRDNNIEFTILPGEGAFYGPKIEFTLHDCLDRAWQC
CCCCCHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCEECCEEEEEHHHHHHHHHCC
GTVQLDYALPSRLGATYVAEDNSRQTPVMIHRAILGSLERFLGILIEEYAGRFPTWLAPM
CCEEECCCCHHHCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHCC
QVVVMNITDKQADYVEEVVKFFKEQGIRASFDLRNEKIGFKIREHTLRRVPYLLVVGDQE
EEEEEECCCCHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCEEEEECCCC
MENKEVAVRTRDGIDLGKMRLEDFATKIHQQISLRSLKLLEE
CCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA