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The map label for this gene is thiH [H]

Identifier: 126174249

GI number: 126174249

Start: 2346834

End: 2347949

Strand: Direct

Name: thiH [H]

Synonym: Sbal_2027

Alternate gene names: 126174249

Gene position: 2346834-2347949 (Clockwise)

Preceding gene: 126174248

Following gene: 126174250

Centisome position: 45.77

GC content: 47.04

Gene sequence:

>1116_bases
ATGAGTTTTGTCGCGGAATTTGCCAAGATCCCACGGGATAAACTGTTACTCGATTTGTATTCTTGCACAGCCCAAGATGT
CGAGCGGGCGTTAGTGAGTCCTGCAGGGGATTTACGTAGCTTACTGGCCTTATTATCACCTGCGGCGGAACCTTATATCG
AAACCATGGCGCAGCACTCTGCTGCGCTTACGCGGCAGCGATTTGGCGCGAATCTGGGCATGTATTTACCGCTATACGTC
TCGAATCTGTGCGCTAATGAGTGTGATTACTGTGGCTTTAGCATGAGTAACAAACTCAAACGCAAGACCTTGAATGAGCT
GGAGTTGATGGCCGAAATGGCCATTATCAAAGATAGGGGCTTCGATTCTATTTTACTGGTGTCGGGTGAGCATGAAACTA
AGGTTGGTATCGATTATTTCAAGCAAATGTTGCCGCTGGTAAAGCAGCAATTTAGCCATTTGGCGATGGAGGTCCAGCCC
ATGAGTGAGGACCATTATTGCCAGTTAGTCGCGCTAGGTTTAGATGCCGTGATGATTTATCAAGAGACCTATCAGCCCGA
GACTTATGCTCGTCACCATTCCCGAGGTAAAAAAATGGATTTTGCTTATCGCTTAGCAACACCTGACAGAGTTGCGGCGG
CGGGCGTTGATAAAATTGGTCTTGGGGTATTACTGGGGCTGGATGATTGGCGTTTAGATGCGTTAATGATGGGATATCAT
CTCGACTATTTAGAGCGACGCTATTGGCGTACCCGCTTTAGTATTTCGCTACCAAGGTTAAGGCCTTGTACTGGCGGGAT
CACCCCAAAAGTCATGCTATCGGATCTAGGTTTAGTGCAAATGATTTGTGCATTTAGACTTTTTAATCAACAGCTTGACA
TCAGCATGTCGACAAGGGAAAGCCCTGAGCTGAGGGATAATCTTTTACCACTTGGGATCACTCAAATCAGTGCGGGCAGT
TCGACACAACCGGGCGGCTATCAAGCGCCTGACAGTCAACTCGATCAATTTGAGATAAGCGATGATCGCAGTGTCGAGCA
AGTTATTGAACAGATGCAACGGCAGGGTTTTAATCCCGTATTTAAGGATTGGGAAGCCAATTGGATCACAGGATAA

Upstream 100 bases:

>100_bases
AAGAGGCGGTGCAATGCGGCAGACGGGCCTTTGAAGCTGGGCTTGGCCGAGTACAAACGGGCGCGGTACATACCAGTCCT
CTAACGGGATTTTTAAATCA

Downstream 100 bases:

>100_bases
ATAAGACCGTAAATAAATGCTGAGAATGCGGATAAAACCTGTACTTTTGCAGGTGATTGGTCAATAATTGACCCATTAAT
CACAGCGTGCAGGAATACCG

Product: thiamine biosynthesis protein ThiH

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: Dehydroglycine synthase; Tyrosine lyase [H]

Number of amino acids: Translated: 371; Mature: 370

Protein sequence:

>371_residues
MSFVAEFAKIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHSAALTRQRFGANLGMYLPLYV
SNLCANECDYCGFSMSNKLKRKTLNELELMAEMAIIKDRGFDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQP
MSEDHYCQLVALGLDAVMIYQETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLDISMSTRESPELRDNLLPLGITQISAGS
STQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPVFKDWEANWITG

Sequences:

>Translated_371_residues
MSFVAEFAKIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHSAALTRQRFGANLGMYLPLYV
SNLCANECDYCGFSMSNKLKRKTLNELELMAEMAIIKDRGFDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQP
MSEDHYCQLVALGLDAVMIYQETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLDISMSTRESPELRDNLLPLGITQISAGS
STQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPVFKDWEANWITG
>Mature_370_residues
SFVAEFAKIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHSAALTRQRFGANLGMYLPLYVS
NLCANECDYCGFSMSNKLKRKTLNELELMAEMAIIKDRGFDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQPM
SEDHYCQLVALGLDAVMIYQETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYHL
DYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLDISMSTRESPELRDNLLPLGITQISAGSS
TQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPVFKDWEANWITG

Specific function: Catalyzes the radical-mediated cleavage of tyrosine to 2-iminoacetate and 4-cresol [H]

COG id: COG1060

COG function: function code HR; Thiamine biosynthesis enzyme ThiH and related uncharacterized enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. ThiH family [H]

Homologues:

Organism=Escherichia coli, GI1790423, Length=366, Percent_Identity=52.7322404371585, Blast_Score=415, Evalue=1e-117,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR007197
- InterPro:   IPR012726 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: NA

Molecular weight: Translated: 41950; Mature: 41819

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFVAEFAKIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHS
CCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
AALTRQRFGANLGMYLPLYVSNLCANECDYCGFSMSNKLKRKTLNELELMAEMAIIKDRG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
FDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQPMSEDHYCQLVALGLDAVMIY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHEEE
QETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
HHHCCCHHHHHHHCCCCCCCEEEEECCCHHHHHCCCCHHCCEEEECCCCHHHHHHHHHHH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLDISMSTRE
HHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEECCCC
SPELRDNLLPLGITQISAGSSTQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPV
CCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCHH
FKDWEANWITG
HHCCCCCCCCC
>Mature Secondary Structure 
SFVAEFAKIPRDKLLLDLYSCTAQDVERALVSPAGDLRSLLALLSPAAEPYIETMAQHS
CHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHHHHHHHHH
AALTRQRFGANLGMYLPLYVSNLCANECDYCGFSMSNKLKRKTLNELELMAEMAIIKDRG
HHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
FDSILLVSGEHETKVGIDYFKQMLPLVKQQFSHLAMEVQPMSEDHYCQLVALGLDAVMIY
CCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHEEE
QETYQPETYARHHSRGKKMDFAYRLATPDRVAAAGVDKIGLGVLLGLDDWRLDALMMGYH
HHHCCCHHHHHHHCCCCCCCEEEEECCCHHHHHCCCCHHCCEEEECCCCHHHHHHHHHHH
LDYLERRYWRTRFSISLPRLRPCTGGITPKVMLSDLGLVQMICAFRLFNQQLDISMSTRE
HHHHHHHHHHHHHEEECCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHCCEECCCC
SPELRDNLLPLGITQISAGSSTQPGGYQAPDSQLDQFEISDDRSVEQVIEQMQRQGFNPV
CCHHHHCCCCCCEEEECCCCCCCCCCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCHH
FKDWEANWITG
HHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8432721; 8265357; 9278503; 10082377 [H]